(Circular) (Six-base) MAPSORT of: pFN21AB5842.seq Check: 8656 from: 1 to: 6154 pFN21AB5842 6154 bp With 182 enzymes: SgfI * August 27, 2010 15:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3964 278 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3497 1937 Size: 1560 4594 Acc65I G'GTAC_C Cuts at: 3475 3475 Size: 6154 AclI AA'CG_TT Cuts at: 1563 4281 4654 1563 Size: 2718 373 3063 Fragments arranged by size: 3063 2718 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 2942 2984 4217 5601 5869 1632 Size: 872 438 42 1233 1384 268 1917 Fragments arranged by size: 1917 1384 1233 872 438 268 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5957 AflIII A'CryG_T Cuts at: 1203 5053 6030 1203 Size: 3850 977 1327 Fragments arranged by size: 3850 1327 977 AgeI A'CCGG_T Cuts at: 4985 4985 Size: 6154 AhdI GACnn_n'nnGTC Cuts at: 4883 4883 Size: 6154 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6154 AlwNI CAG_nnn'CTG Cuts at: 3897 5469 3897 Size: 1572 4582 ApaLI G'TGCA_C Cuts at: 2651 4211 5367 2651 Size: 1560 1156 3438 Fragments arranged by size: 3438 1560 1156 ApoI r'AATT_y Cuts at: 1493 2334 2550 2869 3463 3689 3841 5950 1493 Size: 841 216 319 594 226 152 2109 1697 Fragments arranged by size: 2109 1697 841 594 319 226 216 152 AseI AT'TA_AT Cuts at: 160 1032 4708 160 Size: 872 3676 1606 Fragments arranged by size: 3676 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 AvaI C'yCGr_G Cuts at: 1870 1943 3189 3479 1870 Size: 73 1246 290 4545 Fragments arranged by size: 4545 1246 290 73 BamHI G'GATC_C Cuts at: 3484 3851 3484 Size: 367 5787 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2447 3475 4930 618 Size: 325 217 627 660 1028 1455 1842 Fragments arranged by size: 1842 1455 1028 660 627 325 217 BanII G_rGCy'C Cuts at: 1391 3473 1391 Size: 2082 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5183 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3101 3135 4345 4379 1903 Size: 34 1164 34 1210 34 3678 Fragments arranged by size: 3678 1210 1164 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4046 5256 4046 Size: 1210 4944 BclI T'GATC_A Cuts at: 1548 2716 3315 1548 Size: 1168 599 4387 Fragments arranged by size: 4387 1168 599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4765 136 Size: 107 122 71 631 605 3093 1525 Fragments arranged by size: 3093 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6149 6149 Size: 6154 BlpI GC'TnA_GC Cuts at: 3301 3571 3301 Size: 270 5884 Bme1580I G_kGCm'C Cuts at: 1790 2111 2655 4215 5371 1790 Size: 321 544 1560 1156 2573 Fragments arranged by size: 2573 1560 1156 544 321 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6154 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2929 3083 3885 Size: 416 53 83 399 24 1512 154 802 Cuts at: 3885 4843 442 Size: 958 1753 Fragments arranged by size: 1753 1512 958 802 416 399 154 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3056 1056 Size: 2000 4154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2118 2977 4814 1239 Size: 879 859 1837 2579 Fragments arranged by size: 2579 1837 879 859 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4279 5144 5442 4279 Size: 865 298 4991 Fragments arranged by size: 4991 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3245 4817 882 Size: 617 1746 1572 2219 Fragments arranged by size: 2219 1746 1572 617 BsaAI yAC'GTr Cuts at: 493 1204 2656 3945 493 Size: 711 1452 1289 2702 Fragments arranged by size: 2702 1452 1289 711 BsaBI GATnn'nnATC Cuts at: 6148 6148 Size: 6154 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3961 4343 4978 275 Size: 53 83 186 936 2428 382 635 1451 Fragments arranged by size: 2428 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4587 4985 5259 5406 5817 4587 Size: 398 274 147 411 4924 Fragments arranged by size: 4924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5929 Fragments arranged by size: 5929 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2115 2390 3022 3368 1186 Size: 929 275 632 346 3972 Fragments arranged by size: 3972 929 632 346 275 BseYI C'CCAG_C Cuts at: 2349 3361 5357 5853 2349 Size: 1012 1996 496 2650 Fragments arranged by size: 2650 1996 1012 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2795 1163 Size: 573 1059 4522 Fragments arranged by size: 4522 1059 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3604 4365 5393 1540 Size: 275 186 1603 761 1028 2301 Fragments arranged by size: 2301 1603 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2655 4215 4300 5371 2012 Size: 643 1560 85 1071 2795 Fragments arranged by size: 2795 1560 1071 643 85 BsmI GAATG_Cn' Cuts at: 2382 3665 3758 2382 Size: 1283 93 4778 Fragments arranged by size: 4778 1283 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2111 2655 3473 4215 4300 5371 Size: 399 222 99 544 818 742 85 1071 Cuts at: 5371 1391 Size: 2174 Fragments arranged by size: 2174 1071 818 742 544 399 222 99 85 BspEI T'CCGG_A Cuts at: 5817 5817 Size: 6154 BspHI T'CATG_A Cuts at: 2865 3136 4043 5773 2865 Size: 271 907 1730 3246 Fragments arranged by size: 3246 1730 907 271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3509 844 Size: 2665 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2445 4041 1394 Size: 429 622 1596 3507 Fragments arranged by size: 3507 1596 622 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2388 2511 4649 4823 65 Size: 1183 1140 123 2138 174 1396 Fragments arranged by size: 2138 1396 1183 1140 174 123 BsrFI r'CCGG_y Cuts at: 1700 1810 4798 4985 1700 Size: 110 2988 187 2869 Fragments arranged by size: 2988 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2854 96 Size: 2758 3396 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6154 BssSI C'ACGA_G Cuts at: 4214 5226 4214 Size: 1012 5142 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6154 BstBI TT'CG_AA Cuts at: 4958 4958 Size: 6154 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6154 BstXI CCAn_nnnn'nTGG Cuts at: 2593 3269 2593 Size: 676 5478 BstYI r'GATC_y Cuts at: 1972 2248 2686 3484 3851 4236 4253 5705 5791 Size: 276 438 798 367 385 17 1452 86 Cuts at: 5791 5803 6149 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 798 438 385 367 346 276 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5602 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5415 Fragments arranged by size: 5415 683 56 BtsI GCAGTG_nn' Cuts at: 2971 3670 4464 4484 2971 Size: 699 794 20 4641 Fragments arranged by size: 4641 794 699 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6154 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6119 DraI TTT'AAA Cuts at: 2232 3458 2232 Size: 1226 4928 DraIII CAC_nnn'GTG Cuts at: 2659 2659 Size: 6154 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5161 809 Size: 507 3845 1802 Fragments arranged by size: 3845 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 3601 4490 Size: 54 1425 50 275 18 183 1588 889 Cuts at: 4490 6119 8 Size: 1629 43 Fragments arranged by size: 1629 1588 1425 889 275 183 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3601 1537 Size: 275 1789 4090 Fragments arranged by size: 4090 1789 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4084 1353 Size: 2731 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4741 5115 5261 387 Size: 1064 138 3152 374 146 1280 Fragments arranged by size: 3152 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2118 2504 2942 2977 2984 4217 4814 Size: 393 486 386 438 35 7 1233 597 Cuts at: 4814 5601 5869 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1233 787 597 486 438 393 386 268 35 7 EcoNI CCTnn'n_nnAGG Cuts at: 2100 2486 2906 2100 Size: 386 420 5348 Fragments arranged by size: 5348 420 386 EcoO109I rG'GnC_Cy Cuts at: 2072 2072 Size: 6154 EcoRI G'AATT_C Cuts at: 2869 3463 3841 2869 Size: 594 378 5182 Fragments arranged by size: 5182 594 378 EcoRV GAT'ATC Cuts at: 2410 2410 Size: 6154 FspI TGC'GCA Cuts at: 4660 4660 Size: 6154 HaeII r_GCGC'y Cuts at: 1678 2962 5301 1678 Size: 1284 2339 2531 Fragments arranged by size: 2531 2339 1284 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2261 2293 4795 4827 4869 Size: 32 135 32 689 32 2502 32 42 Cuts at: 4869 4901 1373 Size: 32 2626 Fragments arranged by size: 2626 2502 689 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3498 3744 669 Size: 988 281 1560 246 3079 Fragments arranged by size: 3079 1560 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6154 HpaI GTT'AAC Cuts at: 3744 3744 Size: 6154 KpnI G_GTAC'C Cuts at: 3479 3479 Size: 6154 MfeI C'AATT_G Cuts at: 3753 3753 Size: 6154 MluI A'CGCG_T Cuts at: 6030 6030 Size: 6154 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5268 5452 1305 Size: 3963 184 2007 Fragments arranged by size: 3963 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4332 Fragments arranged by size: 4332 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4112 4471 4630 518 Size: 648 2946 359 159 2042 Fragments arranged by size: 2946 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 2137 3361 3570 4247 5395 5640 1606 Size: 459 72 1224 209 677 1148 245 2120 Fragments arranged by size: 2120 1224 1148 677 459 245 209 72 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5602 NdeI CA'TA_TG Cuts at: 387 2947 3322 387 Size: 2560 375 3219 Fragments arranged by size: 3219 2560 375 NheI G'CTAG_C Cuts at: 1052 3052 1052 Size: 2000 4154 NotI GC'GGCC_GC Cuts at: 3601 3601 Size: 6154 NspI r_CATG'y Cuts at: 1132 1229 3512 5057 1132 Size: 97 2283 1545 2229 Fragments arranged by size: 2283 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2672 1348 Size: 1324 4830 PciI A'CATG_T Cuts at: 5053 5053 Size: 6154 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6154 PfoI T'CCnGG_A Cuts at: 2682 2682 Size: 6154 PmeI GTTT'AAAC Cuts at: 3458 3458 Size: 6154 PmlI CAC'GTG Cuts at: 2656 2656 Size: 6154 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4223 4255 5736 5768 4223 Size: 32 1481 32 4609 Fragments arranged by size: 4609 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6154 PsiI TTA'TAA Cuts at: 2036 3724 2036 Size: 1688 4466 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2520 2552 2520 Size: 32 6122 PstI C_TGCA'G Cuts at: 830 3506 830 Size: 2676 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6154 PvuII CAG'CTG Cuts at: 2065 2137 3361 2065 Size: 72 1224 4858 Fragments arranged by size: 4858 1224 72 SalI G'TCGA_C Cuts at: 1936 3496 1936 Size: 1560 4594 SbfI CC_TGCA'GG Cuts at: 3506 3506 Size: 6154 ScaI AGT'ACT Cuts at: 2939 4402 6114 2939 Size: 1463 1712 2979 Fragments arranged by size: 2979 1712 1463 SexAI A'CCwGG_T Cuts at: 2206 2206 Size: 6154 SfcI C'TryA_G Cuts at: 826 1045 2537 3435 3502 4637 5318 5509 826 Size: 219 1492 898 67 1135 681 191 1471 Fragments arranged by size: 1492 1471 1135 898 681 219 191 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6154 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6154 SmaI CCC'GGG Cuts at: 1872 3481 1872 Size: 1609 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4258 5159 5421 820 Size: 197 926 2315 901 262 1553 Fragments arranged by size: 2315 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3945 493 Size: 3452 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6154 SphI G_CATG'C Cuts at: 1132 3512 1132 Size: 2380 3774 SspI AAT'ATT Cuts at: 5 52 4078 5 Size: 47 4026 2081 Fragments arranged by size: 4026 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6154 StyI C'CwwG_G Cuts at: 513 1065 3593 513 Size: 552 2528 3074 Fragments arranged by size: 3074 2528 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4181 4198 4351 4536 4981 325 Size: 1255 2601 17 153 185 445 1498 Fragments arranged by size: 2601 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2854 2937 2968 Size: 274 80 33 51 1443 877 83 31 Cuts at: 2968 4400 6112 96 Size: 1432 1712 138 Fragments arranged by size: 1712 1443 1432 877 274 138 83 80 51 33 31 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6114 6146 6114 Size: 32 6122 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6154 XbaI T'CTAG_A Cuts at: 3490 3490 Size: 6154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6154 XmaI C'CCGG_G Cuts at: 1870 3479 1870 Size: 1609 4545 XmnI GAAnn'nnTTC Cuts at: 4283 4283 Size: 6154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3962 276 Size: 53 83 186 936 2428 2468 Fragments arranged by size: 2468 2428 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI