Restriction map for
pFN21AB5836
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# NO CUT ENZYMES ( 142 enzymes) #
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Acc65I AccI AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BclI BcnI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRV EcoT22I FbaI
FseI FspI HapII HgaI HindIII
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 15 GACGTC GACGT^C
AcyI 15 GACGTC G[GA]^CG[CT]C
AflII 291 CTTAAG C^TTAAG
AluI 42 AGCT AG^CT
AlwI 184 GATCC GGATC(4/5)
AseI 280 ATTAAT AT^TAAT
AsnI 280 ATTAAT AT^TAAT
AvaII 541 GGTCC G^G[AT]CC
BalI 932 TGGCCA TGG^CCA
BanII 1055 GGGCTC G[GA]GC[CT]^C
BbvCI 43 GCTGAGG CCTCAGC(-5/-2)
BciVI 1210 GGATAC GTATCC(6/5)
BfrI 291 CTTAAG C^TTAAG
BglII 816 AGATCT A^GATCT
BmyI 1055 GGGCTC G[AGT]GC[ACT]^C
BpmI 134 CTGGAG CTGGAG(16/14)
BsaHI 15 GACGTC G[GA]^CG[CT]C
BsmAI 1026 GAGAC GTCTC(1/5)
Bsp1286I 1055 GGGCTC G[AGT]GC[ACT]^C
BsrBI 569 GAGCGG CCGCTC(-3/-3)
BstEII 651 GGTGACC G^GT[ACGT]ACC
BstPI 651 GGTGACC G^GT[ACGT]ACC
BstXI 961 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 541 GGTCC G^G[ACGT]CC
DraI 528 TTTAAA TTT^AAA
DsaI 140 CCATGG C^C[GA][CT]GG
EcoNI 775 CCTGTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 651 GGTGACC G^GT[ACGT]ACC
FauI 571 GCGGG CCCGC(4/6)
HaeII 1231 GGCGCT [GA]GCGC^[CT]
Hin1I 15 GACGTC G[GA]^CG[CT]C
HincII 1398 GTTAAC GT[CT]^[GA]AC
HindII 1398 GTTAAC GT[CT]^[GA]AC
HpaI 1398 GTTAAC GTT^AAC
MaeII 16 ACGT A^CGT
MluNI 932 TGGCCA TGG^CCA
MscI 932 TGGCCA TGG^CCA
MspA1I 41 CAGCTG C[AC]G^C[GT]G
NcoI 140 CCATGG C^CATGG
PacI 364 TTAATTAA TTAAT^TAA
PshAI 1306 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 280 ATTAAT AT^TAAT
PstI 87 CTGCAG CTGCA^G
PvuII 41 CAGCTG CAG^CTG
Sau96I 541 GGTCC G^G[ACGT]CC
ScaI 1358 AGTACT AGT^ACT
SfcI 87 CTGCAG C^T[GA][CT]AG
SspI 1046 AATATT AAT^ATT
StuI 1062 AGGCCT AGG^CCT
TaqI 1314 TCGA T^CGA
TthHB8I 1314 TCGA T^CGA
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccII 12 CGCG CG^CG
27 CGCG
AfaI 1154 GTAC GT^AC
1359 GTAC
BbvI 1329 GCAGC GCAGC(8/12)
850 GCTGC
BsmFI 426 GTCCC GGGAC(10/14)
958 GTCCC
BsrI 854 CCAGT ACTGG(1/-1)
1320 CCAGT
BssKI 31 CCTGG ^CC[ACGT]GG
1246 CCTGG
BstNI 31 CCTGG CC^[AT]GG
1246 CCTGG
BstUI 12 CGCG CG^CG
27 CGCG
BstYI 183 AGATCC [GA]^GATC[CT]
816 AGATCT
CfoI 28 GCGC GCG^C
1232 GCGC
EaeI 101 TGGCCG [CT]^GGCC[GA]
932 TGGCCA
EarI 346 GAAGAG CTCTTC(1/4)
1192 GAAGAG
EcoRI 399 GAATTC G^AATTC
1078 GAATTC
EcoRII 31 CCTGG ^CC[AT]GG
1246 CCTGG
HhaI 28 GCGC GCG^C
1232 GCGC
HinP1I 28 GCGC G^CGC
1232 GCGC
Ksp632I 346 GAAGAG CTCTTC(1/4)
1192 GAAGAG
MflI 183 AGATCC [GA]^GATC[CT]
816 AGATCT
MvaI 31 CCTGG CC^[AT]GG
1246 CCTGG
MvnI 12 CGCG CG^CG
27 CGCG
MwoI 4 GCGACCTCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
21 GCCGCACGCGC
NlaIII 141 CATG CATG^
1198 CATG
NlaIV 136 GGAGCC GG[ACGT]^[ACGT]CC
971 GGAACC
RsaI 1154 GTAC GT^AC
1359 GTAC
SapI 346 GAAGAGC GCTCTTC(1/4)
1192 GAAGAGC
ScrFI 31 CCTGG CC^[ACGT]GG
1246 CCTGG
SmlI 240 CTCAAG C^T[CT][GA]AG
291 CTTAAG
TseI 850 GCTGC G^C[AT]GC
1329 GCAGC
Tsp45I 652 GTGAC ^GT[GC]AC
1007 GTCAC
TspRI 698 CAGTG CA[GC]TG[ACGT][ACGT]^
1073 CAGTG
XhoII 183 AGATCC [GA]^GATC[CT]
816 AGATCT
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AcsI ApoI Asp700I BfaI
BsaJI BsiYI BslI BstF5I Cac8I
DdeI DpnII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI NdeII Sau3AI SfaNI StyI
TfiI Tru9I Tsp509I XmnI