Restriction map for
pFN21AB5822
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BamHI
BbeI BcgI BclI BfrI BglII
BlnI BsaBI BsiEI BsiWI BspDI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NarI
NdeI NheI NotI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 1055 TCCGGA T^CCGGA
Alw44I 1174 GTGCAC G^TGCAC
ApaLI 1174 GTGCAC G^TGCAC
AspI 703 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 221 TGCGCA TGC^GCA
BbsI 67 GTCTTC GAAGAC(2/6)
BbvCI 1291 GCTGAGG CCTCAGC(-5/-2)
BciVI 1145 GTATCC GTATCC(6/5)
BfaI 1253 CTAG C^TAG
BlpI 1216 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1216 GCTGAGC GC^T[ACGT]AGC
BpuAI 67 GTCTTC GAAGAC(2/6)
BsaI 493 GAGACC GGTCTC(1/5)
BsaWI 1055 TCCGGA [AT]^CCGG[AT]
BseAI 1055 TCCGGA T^CCGGA
BsmBI 936 GAGACG CGTCTC(1/5)
BsmI 977 GAATGC GAATGC(1/-1)
Bsp1407I 779 TGTACA T^GTACA
BspEI 1055 TCCGGA T^CCGGA
BsrBI 898 GAGCGG CCGCTC(-3/-3)
BsrFI 189 GCCGGC [GA]^CCGG[CT]
BsrGI 779 TGTACA T^GTACA
BstYI 1494 GGATCT [GA]^GATC[CT]
CelII 1216 GCTGAGC GC^T[ACGT]AGC
Cfr10I 189 GCCGGC [GA]^CCGG[CT]
DrdI 790 GACCCCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 888 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 292 ATGCAT ATGCA^T
FspI 221 TGCGCA TGC^GCA
HaeII 186 AGCGCC [GA]GCGC^[CT]
MaeI 1253 CTAG C^TAG
MflI 1494 GGATCT [GA]^GATC[CT]
MroI 1055 TCCGGA T^CCGGA
MseI 925 TTAA T^TAA
NaeI 189 GCCGGC GCC^GGC
NgoMIV 189 GCCGGC G^CCGGC
NsiI 292 ATGCAT ATGCA^T
PaeR7I 1169 CTCGAG C^TCGAG
PflFI 703 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1519 GACTC GAGTC(4/5)
PshAI 1420 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 451 CTGCAG CTGCA^G
PvuII 7 CAGCTG CAG^CTG
SacI 862 GAGCTC GAGCT^C
SfcI 451 CTGCAG C^T[GA][CT]AG
SfiI 1572 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1431 CCCGGG CCC^GGG
SspBI 779 TGTACA T^GTACA
TaqI 1170 TCGA T^CGA
TfiI 432 GAATC G^A[AT]TC
Tru9I 925 TTAA T^TAA
Tsp509I 927 AATT ^AATT
Tth111I 703 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1170 TCGA T^CGA
XhoI 1169 CTCGAG C^TCGAG
XhoII 1494 GGATCT [GA]^GATC[CT]
XmaCI 1431 CCCGGG C^CCGGG
XmaI 1431 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 204 GGCGTC G[GA]^CG[CT]C
817 GGCGTC
AfaI 483 GTAC GT^AC
780 GTAC
AflIII 140 ACACGT A^C[GA][CT]GT
585 ACACGT
ApaI 884 GGGCCC GGGCC^C
1263 GGGCCC
BalI 632 TGGCCA TGG^CCA
890 TGGCCA
BanI 1024 GGCACC G^G[CT][GA]CC
1452 GGCACC
BbrPI 141 CACGTG CAC^GTG
586 CACGTG
BglI 196 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1573 GCCTCCAAGGC
BsaAI 141 CACGTG [CT]AC^GT[GA]
586 CACGTG
BsaHI 204 GGCGTC G[GA]^CG[CT]C
817 GGCGTC
BsgI 640 CTGCAC GTGCAG(16/14)
415 GTGCAG
BsrI 94 CCAGT ACTGG(1/-1)
378 CCAGT
BstXI 995 CCAATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAGGGAGCTGG
DsaI 200 CCATGG C^C[GA][CT]GG
629 CCATGG
EarI 44 CTCTTC CTCTTC(1/4)
1258 GAAGAG
Hin1I 204 GGCGTC G[GA]^CG[CT]C
817 GGCGTC
HinfI 432 GAATC G^A[ACGT]TC
1519 GACTC
Ksp632I 44 CTCTTC CTCTTC(1/4)
1258 GAAGAG
MluNI 632 TGGCCA TGG^CCA
890 TGGCCA
MscI 632 TGGCCA TGG^CCA
890 TGGCCA
NcoI 200 CCATGG C^CATGG
629 CCATGG
PmaCI 141 CACGTG CAC^GTG
586 CACGTG
PmlI 141 CACGTG CAC^GTG
586 CACGTG
RsaI 483 GTAC GT^AC
780 GTAC
SmlI 1033 CTCAAG C^T[CT][GA]AG
1169 CTCGAG
SphI 224 GCATGC GCATG^C
272 GCATGC
StuI 1037 AGGCCT AGG^CCT
1571 AGGCCT
XcmI 620 CCAATAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1487 CCAAGAAGGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PpuMI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
Tsp45I TspRI Van91I