(Circular) (Six-base) MAPSORT of: pFN21AB5822.seq Check: 4832 from: 1 to: 6334 pFN21AB5822 6334 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3226 3226 Size: 6334 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3376 3592 4397 5781 6049 1632 Size: 1744 216 805 1384 268 1917 Fragments arranged by size: 1917 1744 1384 805 268 216 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 2145 2590 5233 6210 1203 Size: 942 445 2643 977 1327 Fragments arranged by size: 2643 1327 977 942 445 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2240 1184 Size: 1056 5278 AlwNI CAG_nnn'CTG Cuts at: 2128 2838 2879 3160 3253 4077 5649 2128 Size: 710 41 281 93 824 1572 2813 Fragments arranged by size: 2813 1572 824 710 281 93 41 ApaI G_GGCC'C Cuts at: 2893 3272 2893 Size: 379 5955 ApaLI G'TGCA_C Cuts at: 3179 4391 5547 3179 Size: 1212 1156 3966 Fragments arranged by size: 3966 1212 1156 ApoI r'AATT_y Cuts at: 1493 3643 3869 4021 6130 1493 Size: 2150 226 152 2109 1697 Fragments arranged by size: 2150 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 3174 3436 3572 3659 1870 Size: 73 1231 262 136 87 4545 Fragments arranged by size: 4545 1231 262 136 87 73 BamHI G'GATC_C Cuts at: 3664 4031 3664 Size: 367 5967 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3029 3457 3655 5110 618 Size: 325 217 627 1242 428 198 1455 1842 Fragments arranged by size: 1842 1455 1242 627 428 325 217 198 BanII G_rGCy'C Cuts at: 1391 2067 2546 2871 2893 3272 3653 1391 Size: 676 479 325 22 379 381 4072 Fragments arranged by size: 4072 676 479 381 379 325 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2065 928 Size: 971 166 5197 Fragments arranged by size: 5197 971 166 BbvCI CC'TCA_GC Cuts at: 3297 3297 Size: 6334 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4525 4559 1903 Size: 34 2588 34 3678 Fragments arranged by size: 3678 2588 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3161 4226 5436 3161 Size: 1065 1210 4059 Fragments arranged by size: 4059 1210 1065 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BfrBI ATG'CAT Cuts at: 2299 2299 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2207 3584 4945 Size: 107 122 71 631 605 535 1377 1361 Cuts at: 4945 136 Size: 1525 Fragments arranged by size: 1525 1377 1361 631 605 535 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 3222 3751 3222 Size: 529 5805 Bme1580I G_kGCm'C Cuts at: 1790 2893 3032 3183 3272 4395 5551 1790 Size: 1103 139 151 89 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 1103 151 139 89 BmgBI CAC'GTC Cuts at: 1318 3013 1318 Size: 1695 4639 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2093 4065 5023 Size: 416 53 83 399 24 676 1972 958 Cuts at: 5023 442 Size: 1753 Fragments arranged by size: 1972 1753 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3124 3124 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 3119 3140 3182 4994 1239 Size: 1214 666 21 42 1812 2579 Fragments arranged by size: 2579 1812 1214 666 42 21 Bpu10I CC'TnA_GC Cuts at: 3297 3312 3404 3297 Size: 15 92 6227 Fragments arranged by size: 6227 92 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3023 4459 5324 5622 3023 Size: 1436 865 298 3735 Fragments arranged by size: 3735 1436 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2492 4997 882 Size: 617 993 2505 2219 Fragments arranged by size: 2505 2219 993 617 BsaAI yAC'GTr Cuts at: 493 1204 2148 2593 4125 493 Size: 711 944 445 1532 2702 Fragments arranged by size: 2702 1532 944 711 445 BsaBI GATnn'nnATC Cuts at: 6328 6328 Size: 6334 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2210 2823 4141 4523 Size: 53 83 186 936 677 613 1318 382 Cuts at: 4523 5158 275 Size: 635 1451 Fragments arranged by size: 1451 1318 936 677 635 613 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3060 4767 5165 5439 5586 5997 3060 Size: 1707 398 274 147 411 3397 Fragments arranged by size: 3397 1707 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 2477 2507 2639 Size: 30 165 30 729 30 315 30 132 Cuts at: 2639 2669 1178 Size: 30 4843 Fragments arranged by size: 4843 729 315 165 132 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2068 2153 2276 2593 3632 1186 Size: 882 85 123 317 1039 3888 Fragments arranged by size: 3888 1039 882 317 123 85 BseYI C'CCAG_C Cuts at: 2533 3231 3289 3360 3482 5537 6033 2533 Size: 698 58 71 122 2055 496 2834 Fragments arranged by size: 2834 2055 698 496 122 71 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2441 2630 1163 Size: 573 705 189 4867 Fragments arranged by size: 4867 705 573 189 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3784 4545 5573 1540 Size: 275 186 1783 761 1028 2301 Fragments arranged by size: 2301 1783 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2623 2871 3183 4395 4480 5551 2623 Size: 248 312 1212 85 1071 3406 Fragments arranged by size: 3406 1212 1071 312 248 85 BsmI GAATG_Cn' Cuts at: 2988 3845 3938 2988 Size: 857 93 5384 Fragments arranged by size: 5384 857 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2935 2935 Size: 6334 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2546 2623 2871 2893 3032 3183 Size: 399 277 479 77 248 22 139 151 Cuts at: 3183 3272 3653 4395 4480 5551 1391 Size: 89 381 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 479 399 381 277 248 151 139 89 85 77 22 BspEI T'CCGG_A Cuts at: 3060 5997 3060 Size: 2937 3397 BspHI T'CATG_A Cuts at: 4223 5953 4223 Size: 1730 4604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 3087 3226 3689 844 Size: 1325 918 139 463 3489 Fragments arranged by size: 3489 1325 918 463 139 BsrBI CCG'CTC Cuts at: 1394 1823 2905 4221 1394 Size: 429 1082 1316 3507 Fragments arranged by size: 3507 1316 1082 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2194 4978 5165 1700 Size: 110 384 2784 187 2869 Fragments arranged by size: 2869 2784 384 187 110 BsrGI T'GTAC_A Cuts at: 96 2784 96 Size: 2688 3646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6334 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 2305 2838 3253 794 Size: 1445 66 533 415 3875 Fragments arranged by size: 3875 1445 533 415 66 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstXI CCAn_nnnn'nTGG Cuts at: 3007 3097 3007 Size: 90 6244 BstYI r'GATC_y Cuts at: 1972 3499 3664 4031 4416 4433 5885 5971 5983 Size: 1527 165 367 385 17 1452 86 12 Cuts at: 5983 6329 1972 Size: 346 1977 Fragments arranged by size: 1977 1527 1452 385 367 346 165 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2205 2634 513 Size: 552 1140 429 4213 Fragments arranged by size: 4213 1140 552 429 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3639 487 Size: 683 56 2413 3182 Fragments arranged by size: 3182 2413 683 56 BtsI GCAGTG_nn' Cuts at: 3317 3527 3850 4644 4664 3317 Size: 210 323 794 20 4987 Fragments arranged by size: 4987 794 323 210 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2801 5341 809 Size: 507 1485 2540 1802 Fragments arranged by size: 2540 1802 1485 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2358 2637 2895 Size: 54 1425 50 275 18 528 279 258 Cuts at: 2895 3781 4670 6299 8 Size: 886 889 1629 43 Fragments arranged by size: 1629 1425 889 886 528 279 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3781 1537 Size: 275 1969 4090 Fragments arranged by size: 4090 1969 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2055 3258 4264 1353 Size: 702 1203 1006 3423 Fragments arranged by size: 3423 1203 1006 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4921 5295 5441 387 Size: 1064 138 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2869 2869 Size: 6334 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 3119 3140 3182 3376 3592 4397 Size: 393 821 666 21 42 194 216 805 Cuts at: 4397 4994 5781 6049 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 821 805 787 666 597 393 268 216 194 42 21 EcoNI CCTnn'n_nnAGG Cuts at: 2897 2897 Size: 6334 EcoO109I rG'GnC_Cy Cuts at: 2889 2890 3076 3268 3280 3301 3431 2889 Size: 1 186 192 12 21 130 5792 Fragments arranged by size: 5792 192 186 130 21 12 1 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FspI TGC'GCA Cuts at: 2228 4840 2228 Size: 2612 3722 HaeII r_GCGC'y Cuts at: 1678 2195 5481 1678 Size: 517 3286 2531 Fragments arranged by size: 3286 2531 517 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2477 2509 3157 3189 4975 Size: 32 135 32 905 32 648 32 1786 Cuts at: 4975 5007 5049 5081 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1786 905 648 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3678 3924 669 Size: 988 281 1740 246 3079 Fragments arranged by size: 3079 1740 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 2682 5448 5632 1305 Size: 1350 27 2766 184 2007 Fragments arranged by size: 2766 2007 1350 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 2639 2897 10 Size: 54 1425 343 807 258 3447 Fragments arranged by size: 3447 1425 807 343 258 54 MslI CAynn'nnrTG Cuts at: 518 1166 2639 2778 3215 4292 4651 4810 518 Size: 648 1473 139 437 1077 359 159 2042 Fragments arranged by size: 2042 1473 1077 648 437 359 159 139 MspA1I CmG'CkG Cuts at: 1606 2014 2269 2609 3346 3538 3750 4427 5575 Size: 408 255 340 737 192 212 677 1148 Cuts at: 5575 5820 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 737 677 408 340 255 245 212 192 NaeI GCC'GGC Cuts at: 2196 2196 Size: 6334 NcoI C'CATG_G Cuts at: 513 1065 2205 2634 513 Size: 552 1140 429 4213 Fragments arranged by size: 4213 1140 552 429 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NgoMIV G'CCGG_C Cuts at: 2194 2194 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 3781 3781 Size: 6334 NsiI A_TGCA'T Cuts at: 2301 2301 Size: 6334 NspI r_CATG'y Cuts at: 1132 1229 2233 2281 2308 3692 5237 1132 Size: 97 1004 48 27 1384 1545 2229 Fragments arranged by size: 2229 1545 1384 1004 97 48 27 PasI CC'CwG_GG Cuts at: 1348 2539 2573 3272 1348 Size: 1191 34 699 4410 Fragments arranged by size: 4410 1191 699 34 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 3160 3238 3385 1108 Size: 2052 78 147 4057 Fragments arranged by size: 4057 2052 147 78 PfoI T'CCnGG_A Cuts at: 3153 3516 3153 Size: 363 5971 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PmlI CAC'GTG Cuts at: 2148 2593 2148 Size: 445 5889 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3076 3301 3431 3076 Size: 225 130 5979 Fragments arranged by size: 5979 225 130 PshAI GACnn'nnGTC Cuts at: 1613 3429 1613 Size: 1816 4518 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PspOMI G'GGCC_C Cuts at: 2889 3268 2889 Size: 379 5955 PspXI vC'TCGA_Gb Cuts at: 3174 3174 Size: 6334 PstI C_TGCA'G Cuts at: 830 2460 3686 830 Size: 1630 1226 3478 Fragments arranged by size: 3478 1630 1226 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2014 2014 Size: 6334 SacI G_AGCT'C Cuts at: 2871 2871 Size: 6334 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SanDI GG'GwC_CC Cuts at: 3431 3431 Size: 6334 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 4582 6294 4582 Size: 1712 4622 SfcI C'TryA_G Cuts at: 826 1045 2456 3682 4817 5498 5689 826 Size: 219 1411 1226 1135 681 191 1471 Fragments arranged by size: 1471 1411 1226 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3584 3584 Size: 6334 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 3438 3661 1872 Size: 1566 223 4545 Fragments arranged by size: 4545 1566 223 SmlI C'TyrA_G Cuts at: 820 1017 1943 3038 3174 4438 5339 5601 820 Size: 197 926 1095 136 1264 901 262 1553 Fragments arranged by size: 1553 1264 1095 926 901 262 197 136 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 2233 2281 3692 1132 Size: 1101 48 1411 3774 Fragments arranged by size: 3774 1411 1101 48 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 3044 3578 1517 Size: 1527 534 4273 Fragments arranged by size: 4273 1527 534 StyI C'CwwG_G Cuts at: 513 1065 2037 2205 2634 2716 2898 2994 3582 Size: 552 972 168 429 82 182 96 588 Cuts at: 3582 3773 513 Size: 191 3074 Fragments arranged by size: 3074 972 588 552 429 191 182 168 96 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3374 4361 4378 4531 4716 5161 325 Size: 1255 1794 987 17 153 185 445 1498 Fragments arranged by size: 1794 1498 1255 987 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2784 4580 6292 Size: 274 80 33 51 1443 807 1796 1712 Cuts at: 6292 96 Size: 138 Fragments arranged by size: 1796 1712 1443 807 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2132 2164 2582 2614 2639 2671 6294 6326 2132 Size: 32 418 32 25 32 3623 32 2140 Fragments arranged by size: 3623 2140 418 32 32 32 32 25 Tth111I GACn'n_nGTC Cuts at: 1316 2711 1316 Size: 1395 4939 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2632 3499 2632 Size: 867 5467 XhoI C'TCGA_G Cuts at: 1943 3174 1943 Size: 1231 5103 XmaI C'CCGG_G Cuts at: 1870 3436 3659 1870 Size: 1566 223 4545 Fragments arranged by size: 4545 1566 223 XmnI GAAnn'nnTTC Cuts at: 4463 4463 Size: 6334 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI