Restriction map for
pFN21AB5822
(Linear) (Six-base) MAPSORT of: KIBB5822.seq Check: 1504 from: 1 to: 1629
KIBB5822.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1221 1629
Size: 1221 408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1371 1587 1629
Size: 1371 216 42
Fragments arranged by size:
1371 216 42
AflIII A'CryG_T
Cuts at: 0 140 585 1629
Size: 140 445 1044
Fragments arranged by size:
1044 445 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 1629
Size: 235 1394
AlwNI CAG_nnn'CTG
Cuts at: 0 123 833 874 1155 1248 1629
Size: 123 710 41 281 93 381
Fragments arranged by size:
710 381 281 123 93 41
ApaI G_GGCC'C
Cuts at: 0 888 1267 1629
Size: 888 379 362
Fragments arranged by size:
888 379 362
ApaLI G'TGCA_C
Cuts at: 0 1174 1629
Size: 1174 455
AvaI C'yCGr_G
Cuts at: 0 1169 1431 1567 1629
Size: 1169 262 136 62
Fragments arranged by size:
1169 262 136 62
BanI G'GyrC_C
Cuts at: 0 1024 1452 1629
Size: 1024 428 177
Fragments arranged by size:
1024 428 177
BanII G_rGCy'C
Cuts at: 0 62 541 866 888 1267 1629
Size: 62 479 325 22 379 362
Fragments arranged by size:
479 379 362 325 62 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 60 1629
Size: 60 1569
BbvCI CC'TCA_GC
Cuts at: 0 1292 1629
Size: 1292 337
BciVI GTATCCnnnnn_n'
Cuts at: 0 1156 1629
Size: 1156 473
BfrBI ATG'CAT
Cuts at: 0 294 1629
Size: 294 1335
BglI GCCn_nnn'nGGC
Cuts at: 0 202 1579 1629
Size: 202 1377 50
Fragments arranged by size:
1377 202 50
BlpI GC'TnA_GC
Cuts at: 0 1217 1629
Size: 1217 412
Bme1580I G_kGCm'C
Cuts at: 0 888 1027 1178 1267 1629
Size: 888 139 151 89 362
Fragments arranged by size:
888 362 151 139 89
BmgBI CAC'GTC
Cuts at: 0 1008 1629
Size: 1008 621
BmrI ACTGGGnnnn_n'
Cuts at: 0 88 1629
Size: 88 1541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1119 1629
Size: 1119 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1114 1135 1177 1629
Size: 448 666 21 42 452
Fragments arranged by size:
666 452 448 42 21
Bpu10I CC'TnA_GC
Cuts at: 0 1292 1307 1399 1629
Size: 1292 15 92 230
Fragments arranged by size:
1292 230 92 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1629
Size: 1018 611
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 1629
Size: 487 1142
BsaAI yAC'GTr
Cuts at: 0 143 588 1629
Size: 143 445 1041
Fragments arranged by size:
1041 445 143
BsaHI Gr'CG_yC
Cuts at: 0 205 818 1629
Size: 205 613 811
Fragments arranged by size:
811 613 205
BsaWI w'CCGG_w
Cuts at: 0 1055 1629
Size: 1055 574
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 127 157 472 502 634 664 1629
Size: 127 30 315 30 132 30 965
Fragments arranged by size:
965 315 132 127 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 63 148 271 588 1627 1629
Size: 63 85 123 317 1039 2
Fragments arranged by size:
1039 317 123 85 63 2
BseYI C'CCAG_C
Cuts at: 0 528 1226 1284 1355 1477 1629
Size: 528 698 58 71 122 152
Fragments arranged by size:
698 528 152 122 71 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 625 1629
Size: 436 189 1004
Fragments arranged by size:
1004 436 189
BsiHKAI G_wGCw'C
Cuts at: 0 618 866 1178 1629
Size: 618 248 312 451
Fragments arranged by size:
618 451 312 248
BsmI GAATG_Cn'
Cuts at: 0 983 1629
Size: 983 646
BsmBI CGTCTCn'nnnn_
Cuts at: 0 930 1629
Size: 930 699
Bsp1286I G_dGCh'C
Cuts at: 0 62 541 618 866 888 1027 1178 1267
Size: 62 479 77 248 22 139 151 89
Cuts at: 1267 1629
Size: 362
Fragments arranged by size:
479 362 248 151 139 89 77 62
22
BspEI T'CCGG_A
Cuts at: 0 1055 1629
Size: 1055 574
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 1082 1221 1629
Size: 164 918 139 408
Fragments arranged by size:
918 408 164 139
BsrBI CCG'CTC
Cuts at: 0 900 1629
Size: 900 729
BsrFI r'CCGG_y
Cuts at: 0 189 1629
Size: 189 1440
BsrGI T'GTAC_A
Cuts at: 0 779 1629
Size: 779 850
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 300 833 1248 1629
Size: 234 66 533 415 381
Fragments arranged by size:
533 415 381 234 66
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1002 1092 1629
Size: 1002 90 537
Fragments arranged by size:
1002 537 90
BstYI r'GATC_y
Cuts at: 0 1494 1629
Size: 1494 135
BtgI C'CryG_G
Cuts at: 0 200 629 1629
Size: 200 429 1000
Fragments arranged by size:
1000 429 200
BtsI GCAGTG_nn'
Cuts at: 0 1312 1522 1629
Size: 1312 210 107
Fragments arranged by size:
1312 210 107
DrdI GACnn_nn'nnGTC
Cuts at: 0 796 1629
Size: 796 833
EaeI y'GGCC_r
Cuts at: 0 353 632 890 1629
Size: 353 279 258 739
Fragments arranged by size:
739 353 279 258
EarI CTCTTCn'nnn_
Cuts at: 0 50 1253 1629
Size: 50 1203 376
Fragments arranged by size:
1203 376 50
EcoICRI GAG'CTC
Cuts at: 0 864 1629
Size: 864 765
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1114 1135 1177 1371 1587 1629
Size: 448 666 21 42 194 216 42
Fragments arranged by size:
666 448 216 194 42 42 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 892 1629
Size: 892 737
EcoO109I rG'GnC_Cy
Cuts at: 0 884 885 1071 1263 1275 1296 1426 1629
Size: 884 1 186 192 12 21 130 203
Fragments arranged by size:
884 203 192 186 130 21 12 1
FspI TGC'GCA
Cuts at: 0 223 1629
Size: 223 1406
HaeII r_GCGC'y
Cuts at: 0 190 1629
Size: 190 1439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 472 504 1152 1184 1629
Size: 472 32 648 32 445
Fragments arranged by size:
648 472 445 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 677 1629
Size: 650 27 952
Fragments arranged by size:
952 650 27
MscI TGG'CCA
Cuts at: 0 634 892 1629
Size: 634 258 737
Fragments arranged by size:
737 634 258
MslI CAynn'nnrTG
Cuts at: 0 634 773 1210 1629
Size: 634 139 437 419
Fragments arranged by size:
634 437 419 139
MspA1I CmG'CkG
Cuts at: 0 9 264 604 1341 1533 1629
Size: 9 255 340 737 192 96
Fragments arranged by size:
737 340 255 192 96 9
NaeI GCC'GGC
Cuts at: 0 191 1629
Size: 191 1438
NcoI C'CATG_G
Cuts at: 0 200 629 1629
Size: 200 429 1000
Fragments arranged by size:
1000 429 200
NgoMIV G'CCGG_C
Cuts at: 0 189 1629
Size: 189 1440
NsiI A_TGCA'T
Cuts at: 0 296 1629
Size: 296 1333
NspI r_CATG'y
Cuts at: 0 228 276 303 1629
Size: 228 48 27 1326
Fragments arranged by size:
1326 228 48 27
PasI CC'CwG_GG
Cuts at: 0 534 568 1267 1629
Size: 534 34 699 362
Fragments arranged by size:
699 534 362 34
PflMI CCAn_nnn'nTGG
Cuts at: 0 1155 1233 1380 1629
Size: 1155 78 147 249
Fragments arranged by size:
1155 249 147 78
PfoI T'CCnGG_A
Cuts at: 0 1148 1511 1629
Size: 1148 363 118
Fragments arranged by size:
1148 363 118
PmlI CAC'GTG
Cuts at: 0 143 588 1629
Size: 143 445 1041
Fragments arranged by size:
1041 445 143
PpuMI rG'GwC_Cy
Cuts at: 0 1071 1296 1426 1629
Size: 1071 225 130 203
Fragments arranged by size:
1071 225 203 130
PshAI GACnn'nnGTC
Cuts at: 0 1424 1629
Size: 1424 205
PspOMI G'GGCC_C
Cuts at: 0 884 1263 1629
Size: 884 379 366
Fragments arranged by size:
884 379 366
PspXI vC'TCGA_Gb
Cuts at: 0 1169 1629
Size: 1169 460
PstI C_TGCA'G
Cuts at: 0 455 1629
Size: 455 1174
PvuII CAG'CTG
Cuts at: 0 9 1629
Size: 9 1620
SacI G_AGCT'C
Cuts at: 0 866 1629
Size: 866 763
SanDI GG'GwC_CC
Cuts at: 0 1426 1629
Size: 1426 203
SfcI C'TryA_G
Cuts at: 0 451 1629
Size: 451 1178
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1579 1629
Size: 1579 50
SmaI CCC'GGG
Cuts at: 0 1433 1629
Size: 1433 196
SmlI C'TyrA_G
Cuts at: 0 1033 1169 1629
Size: 1033 136 460
Fragments arranged by size:
1033 460 136
SphI G_CATG'C
Cuts at: 0 228 276 1629
Size: 228 48 1353
Fragments arranged by size:
1353 228 48
StuI AGG'CCT
Cuts at: 0 1039 1573 1629
Size: 1039 534 56
Fragments arranged by size:
1039 534 56
StyI C'CwwG_G
Cuts at: 0 32 200 629 711 893 989 1577 1629
Size: 32 168 429 82 182 96 588 52
Fragments arranged by size:
588 429 182 168 96 82 52 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1369 1629
Size: 1369 260
TatI w'GTAC_w
Cuts at: 0 779 1629
Size: 779 850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 577 609 634 666 1629
Size: 127 32 418 32 25 32 963
Fragments arranged by size:
963 418 127 32 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 0 706 1629
Size: 706 923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 627 1494 1629
Size: 627 867 135
Fragments arranged by size:
867 627 135
XhoI C'TCGA_G
Cuts at: 0 1169 1629
Size: 1169 460
XmaI C'CCGG_G
Cuts at: 0 1431 1629
Size: 1431 198
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbsI BbvCI BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI
BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NsiI NspI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SanDI SfcI SfiI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BclI BglII BmtI BsaBI BsiEI
BsiWI BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI PacI PciI PmeI PpiI PsiI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI
XmnI ZraI