Restriction map for
pFN21AB5817
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BanI BbeI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstNI BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspA1I MunI MvaI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I StuI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AciI 79 GCGG CCGC(-3/-1)
AcsI 353 GAATTT [GA]^AATT[CT]
AfaI 465 GTAC GT^AC
AflIII 413 ACACGT A^C[GA][CT]GT
ApoI 353 GAATTT [GA]^AATT[CT]
AspHI 629 GAGCTC G[AT]GC[AT]^C
BalI 497 TGGCCA TGG^CCA
BamHI 522 GGATCC G^GATCC
BanII 629 GAGCTC G[GA]GC[CT]^C
BbrPI 414 CACGTG CAC^GTG
BbsI 336 GTCTTC GAAGAC(2/6)
BcnI 518 CCGGG CC^[GC]GG
BmyI 629 GAGCTC G[AGT]GC[ACT]^C
BpuAI 336 GTCTTC GAAGAC(2/6)
BsaAI 414 CACGTG [CT]AC^GT[GA]
BseRI 385 GAGGAG GAGGAG(10/8)
BsgI 559 CTGCAC GTGCAG(16/14)
BsiHKAI 629 GAGCTC G[AT]GC[AT]^C
BsmAI 616 GAGAC GTCTC(1/5)
Bsp1286I 629 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 464 TGTACA T^GTACA
BspMI 127 GCAGGT ACCTGC(4/8)
BsrGI 464 TGTACA T^GTACA
BsrI 261 ACTGG ACTGG(1/-1)
BssKI 518 CCGGG ^CC[ACGT]GG
BstF5I 297 GGATG GGATG(2/0)
BstYI 522 GGATCC [GA]^GATC[CT]
CfoI 489 GCGC GCG^C
DdeI 538 CTGAG C^T[ACGT]AG
DpnII 523 GATC ^GATC
EaeI 497 TGGCCA [CT]^GGCC[GA]
FokI 297 GGATG GGATG(9/13)
HapII 518 CCGG C^CGG
HhaI 489 GCGC GCG^C
HinP1I 489 GCGC G^CGC
HincII 303 GTTGAC GT[CT]^[GA]AC
HindII 303 GTTGAC GT[CT]^[GA]AC
HinfI 12 GACTC G^A[ACGT]TC
HpaII 518 CCGG C^CGG
MaeII 415 ACGT A^CGT
MaeIII 25 GTAAC ^GT[ACGT]AC
MboI 523 GATC ^GATC
MflI 522 GGATCC [GA]^GATC[CT]
MluNI 497 TGGCCA TGG^CCA
MscI 497 TGGCCA TGG^CCA
MslI 430 CATTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 518 CCGG C^CGG
NciI 518 CCGGG CC^[GC]GG
NdeII 523 GATC ^GATC
NlaIV 522 GGATCC GG[ACGT]^[ACGT]CC
NspI 605 GCATGC [GA]CATG^[CT]
PleI 12 GACTC GAGTC(4/5)
PmaCI 414 CACGTG CAC^GTG
PmlI 414 CACGTG CAC^GTG
PstI 283 CTGCAG CTGCA^G
RsaI 465 GTAC GT^AC
SacI 629 GAGCTC GAGCT^C
Sau3AI 523 GATC ^GATC
ScrFI 518 CCGGG CC^[ACGT]GG
SfcI 283 CTGCAG C^T[GA][CT]AG
SphI 605 GCATGC GCATG^C
SspBI 464 TGTACA T^GTACA
SspI 148 AATATT AAT^ATT
TaqI 217 TCGA T^CGA
TthHB8I 217 TCGA T^CGA
XhoII 522 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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AlwI 523 GATCC GGATC(4/5)
522 GGATC
BfaI 196 CTAG C^TAG
364 CTAG
HphI 318 GGTGA GGTGA(8/7)
533 TCACC
MaeI 196 CTAG C^TAG
364 CTAG
MboII 229 GAAGA GAAGA(8/7)
337 TCTTC
MseI 68 TTAA T^TAA
97 TTAA
MwoI 480 GCTCCTGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
558 GCTGCACCAGC
NlaIII 552 CATG CATG^
606 CATG
SfaNI 124 GATGC GCATC(5/9)
594 GCATC
Tru9I 68 TTAA T^TAA
97 TTAA
TspRI 54 CAGTG CA[GC]TG[ACGT][ACGT]^
138 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BpmI BsaJI BsiYI
BslI Cac8I EcoT14I Fnu4HI HaeIII
ItaI MnlI StyI TseI Tsp509I