(Circular) (Six-base) MAPSORT of: pFN21AB5811.seq Check: 6786 from: 1 to: 6127 pFN21AB5811 6127 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2353 3137 3937 278 Size: 53 83 186 936 817 784 800 2468 Fragments arranged by size: 2468 936 817 800 784 186 83 53 AccI GT'mk_AC Cuts at: 1937 2306 3470 1937 Size: 369 1164 4594 Fragments arranged by size: 4594 1164 369 Acc65I G'GTAC_C Cuts at: 3448 3448 Size: 6127 AclI AA'CG_TT Cuts at: 1563 4254 4627 1563 Size: 2691 373 3063 Fragments arranged by size: 3063 2691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2557 3035 3163 3282 4190 5574 5842 1632 Size: 925 478 128 119 908 1384 268 1917 Fragments arranged by size: 1917 1384 925 908 478 268 128 119 AfeI AGC'GCT Cuts at: 2780 2780 Size: 6127 AflII C'TTAA_G Cuts at: 820 1017 3142 820 Size: 197 2125 3805 Fragments arranged by size: 3805 2125 197 AflIII A'CryG_T Cuts at: 1203 5026 6003 1203 Size: 3823 977 1327 Fragments arranged by size: 3823 1327 977 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 3400 4856 3400 Size: 1456 4671 AleI CACnn'nnGTG Cuts at: 1166 3353 1166 Size: 2187 3940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 3870 5442 3870 Size: 1572 4555 ApaLI G'TGCA_C Cuts at: 4184 5340 4184 Size: 1156 4971 ApoI r'AATT_y Cuts at: 1493 2148 3436 3662 3814 5923 1493 Size: 655 1288 226 152 2109 1697 Fragments arranged by size: 2109 1697 1288 655 226 152 AseI AT'TA_AT Cuts at: 160 1032 4681 160 Size: 872 3649 1606 Fragments arranged by size: 3649 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3381 3452 1870 Size: 73 1438 71 4545 Fragments arranged by size: 4545 1438 73 71 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3448 4903 618 Size: 325 217 627 1661 1455 1842 Fragments arranged by size: 1842 1661 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2418 3446 1391 Size: 1027 1028 4072 Fragments arranged by size: 4072 1028 1027 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2284 3137 928 Size: 971 385 853 3918 Fragments arranged by size: 3918 971 853 385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4318 4352 1903 Size: 34 2381 34 3678 Fragments arranged by size: 3678 2381 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4019 5229 4019 Size: 1210 4917 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2700 6122 2700 Size: 3422 2705 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 2925 3152 4188 5344 1790 Size: 1135 227 1036 1156 2573 Fragments arranged by size: 2573 1156 1135 1036 227 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2940 3858 4816 Size: 416 53 83 399 24 1523 918 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1753 1523 958 918 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6127 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2825 3343 3437 4787 1239 Size: 1586 518 94 1350 2579 Fragments arranged by size: 2579 1586 1350 518 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2360 4252 5117 5415 2360 Size: 1892 865 298 3072 Fragments arranged by size: 3072 1892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3305 4790 882 Size: 617 1806 1485 2219 Fragments arranged by size: 2219 1806 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 6121 6121 Size: 6127 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2350 3134 3934 4316 Size: 53 83 186 936 817 784 800 382 Cuts at: 4316 4951 275 Size: 635 1451 Fragments arranged by size: 1451 936 817 800 784 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2474 2504 2528 2558 1178 Size: 30 165 30 1071 30 24 30 4747 Fragments arranged by size: 4747 1071 165 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2549 2789 2830 1186 Size: 1363 240 41 4483 Fragments arranged by size: 4483 1363 240 41 BseYI C'CCAG_C Cuts at: 2354 2557 2968 5330 5826 2354 Size: 203 411 2362 496 2655 Fragments arranged by size: 2655 2362 496 411 203 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2092 2552 2579 1163 Size: 573 356 460 27 4711 Fragments arranged by size: 4711 573 460 356 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2436 4188 4273 5344 2436 Size: 1752 85 1071 3219 Fragments arranged by size: 3219 1752 1071 85 BsmI GAATG_Cn' Cuts at: 3638 3731 3638 Size: 93 6034 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2418 2436 2925 3152 3446 4188 4273 Size: 399 628 18 489 227 294 742 85 Cuts at: 4273 5344 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 628 489 399 294 227 85 18 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2090 2598 2643 3482 844 Size: 1246 508 45 839 3489 Fragments arranged by size: 3489 1246 839 508 45 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3265 4622 4796 65 Size: 1183 2017 1357 174 1396 Fragments arranged by size: 2017 1396 1357 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 4771 4958 1700 Size: 110 275 2686 187 2869 Fragments arranged by size: 2869 2686 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 2475 4931 2475 Size: 2456 3671 BstEII G'GTnAC_C Cuts at: 1184 3421 1184 Size: 2237 3890 BstYI r'GATC_y Cuts at: 1972 2538 2700 2793 3457 3824 4209 4226 5678 Size: 566 162 93 664 367 385 17 1452 Cuts at: 5678 5764 5776 6122 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 664 566 385 367 346 162 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3374 3374 Size: 6127 BtgI C'CryG_G Cuts at: 513 1065 2004 2255 2835 3354 513 Size: 552 939 251 580 519 3286 Fragments arranged by size: 3286 939 580 552 519 251 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5388 Fragments arranged by size: 5388 683 56 BtsI GCAGTG_nn' Cuts at: 2089 2209 3368 3643 4437 4457 2089 Size: 120 1159 275 794 20 3759 Fragments arranged by size: 3759 1159 794 275 120 20 ClaI AT'CG_AT Cuts at: 1554 2370 1554 Size: 816 5311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 3431 3431 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2232 3238 Size: 54 1425 50 275 18 360 42 1006 Cuts at: 3238 3286 3574 4463 6092 8 Size: 48 288 889 1629 43 Fragments arranged by size: 1629 1425 1006 889 360 288 275 54 50 48 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3245 3301 4057 1353 Size: 1892 56 756 3423 Fragments arranged by size: 3423 1892 756 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2557 2825 3035 3163 3282 3343 3437 Size: 393 925 268 210 128 119 61 94 Cuts at: 3437 4190 4787 5574 5842 1239 Size: 753 597 787 268 1524 Fragments arranged by size: 1524 925 787 753 597 393 268 268 210 128 119 94 61 EcoNI CCTnn'n_nnAGG Cuts at: 2912 3142 2912 Size: 230 5897 EcoO109I rG'GnC_Cy Cuts at: 2156 2632 2820 3302 3378 2156 Size: 476 188 482 76 4905 Fragments arranged by size: 4905 482 476 188 76 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3154 3154 Size: 6127 FspI TGC'GCA Cuts at: 4633 4633 Size: 6127 HaeII r_GCGC'y Cuts at: 1678 2406 2782 2939 5274 1678 Size: 728 376 157 2335 2531 Fragments arranged by size: 2531 2335 728 376 157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2204 2236 2320 2352 2504 Size: 32 135 32 632 32 84 32 152 Cuts at: 2504 2528 2536 2560 2840 2872 3386 3418 4768 Size: 24 8 24 280 32 514 32 1350 Cuts at: 4768 4800 4842 4874 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 632 514 280 152 135 84 42 32 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2905 3471 3717 669 Size: 988 281 967 566 246 3079 Fragments arranged by size: 3079 988 967 566 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6127 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 3452 3452 Size: 6127 MfeI C'AATT_G Cuts at: 3153 3726 3153 Size: 573 5554 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 2446 2526 2709 5241 5425 1305 Size: 696 445 80 183 2532 184 2007 Fragments arranged by size: 2532 2007 696 445 184 183 80 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 3288 10 Size: 54 1425 343 360 1096 2849 Fragments arranged by size: 2849 1425 1096 360 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3353 4085 4444 4603 518 Size: 648 2187 732 359 159 2042 Fragments arranged by size: 2187 2042 732 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2248 2557 3097 3543 4220 5368 5613 Size: 512 130 309 540 446 677 1148 245 Cuts at: 5613 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 540 512 446 309 245 130 NaeI GCC'GGC Cuts at: 2087 2087 Size: 6127 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4636 Fragments arranged by size: 4636 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NgoMIV G'CCGG_C Cuts at: 2085 2085 Size: 6127 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6127 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2566 3334 3485 5030 1132 Size: 97 1337 768 151 1545 2229 Fragments arranged by size: 2229 1545 1337 768 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 5026 5026 Size: 6127 PflMI CCAn_nnn'nTGG Cuts at: 1108 2674 3008 3321 1108 Size: 1566 334 313 3914 Fragments arranged by size: 3914 1566 334 313 PfoI T'CCnGG_A Cuts at: 2802 2802 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3055 3087 4196 4228 5709 5741 3055 Size: 32 1109 32 1481 32 3441 Fragments arranged by size: 3441 1481 1109 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2156 2632 2820 3378 2156 Size: 476 188 558 4905 Fragments arranged by size: 4905 558 476 188 PshAI GACnn'nnGTC Cuts at: 1613 2284 2741 1613 Size: 671 457 4999 Fragments arranged by size: 4999 671 457 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PstI C_TGCA'G Cuts at: 830 3479 830 Size: 2649 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2118 2557 3097 2118 Size: 439 540 5148 Fragments arranged by size: 5148 540 439 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SapI GCTCTTCn'nnn_ Cuts at: 3245 3245 Size: 6127 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 3293 4375 6087 3293 Size: 1082 1712 3333 Fragments arranged by size: 3333 1712 1082 SexAI A'CCwGG_T Cuts at: 2907 2907 Size: 6127 SfcI C'TryA_G Cuts at: 826 1045 3475 4610 5291 5482 826 Size: 219 2430 1135 681 191 1471 Fragments arranged by size: 2430 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 3142 4231 5132 5394 820 Size: 197 926 396 803 1089 901 262 1553 Fragments arranged by size: 1553 1089 926 901 803 396 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 2566 3334 3485 1132 Size: 1434 768 151 3774 Fragments arranged by size: 3774 1434 768 151 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2004 3067 3566 513 Size: 552 939 1063 499 3074 Fragments arranged by size: 3074 1063 939 552 499 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4154 4171 4324 4509 4954 325 Size: 1255 2574 17 153 185 445 1498 Fragments arranged by size: 2574 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 3291 4373 Size: 274 80 33 51 1443 510 804 1082 Cuts at: 4373 6085 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1082 804 510 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2474 2506 3142 3174 6087 6119 2474 Size: 32 636 32 2913 32 2482 Fragments arranged by size: 2913 2482 636 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2222 2909 1316 Size: 906 687 4534 Fragments arranged by size: 4534 906 687 XbaI T'CTAG_A Cuts at: 2048 3463 2048 Size: 1415 4712 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2554 2870 3101 2554 Size: 316 231 5580 Fragments arranged by size: 5580 316 231 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6127 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2351 3135 3935 276 Size: 53 83 186 936 817 784 800 2468 Fragments arranged by size: 2468 936 817 800 784 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI