(Circular) (Six-base) MAPSORT of: pFN21AB7979.seq Check: 9847 from: 1 to: 6001 pFN21AB7979 6001 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2424 3344 1937 Size: 487 920 4594 Fragments arranged by size: 4594 920 487 Acc65I G'GTAC_C Cuts at: 2613 3322 2613 Size: 709 5292 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2048 4064 5448 5716 1632 Size: 416 2016 1384 268 1917 Fragments arranged by size: 2016 1917 1384 416 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 3099 4900 5877 1203 Size: 1896 1801 977 1327 Fragments arranged by size: 1896 1801 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AlwNI CAG_nnn'CTG Cuts at: 3052 3055 3244 3744 5316 3052 Size: 3 189 500 1572 3737 Fragments arranged by size: 3737 1572 500 189 3 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 2396 3310 3536 3688 5797 1493 Size: 903 914 226 152 2109 1697 Fragments arranged by size: 2109 1697 914 903 226 152 AseI AT'TA_AT Cuts at: 160 1032 2630 4555 160 Size: 872 1598 1925 1606 Fragments arranged by size: 1925 1606 1598 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2613 2804 3322 4777 618 Size: 325 217 627 826 191 518 1455 1842 Fragments arranged by size: 1842 1455 826 627 518 325 217 191 BanII G_rGCy'C Cuts at: 1391 3320 1391 Size: 1929 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2118 928 Size: 971 219 4811 Fragments arranged by size: 4811 971 219 BbvCI CC'TCA_GC Cuts at: 3152 3152 Size: 6001 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2258 2292 2775 2809 4192 4226 1903 Size: 34 321 34 483 34 1383 34 3678 Fragments arranged by size: 3678 1383 483 321 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 2857 1548 Size: 1309 4692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2798 3156 4612 Size: 107 122 71 631 605 1126 358 1456 Cuts at: 4612 136 Size: 1525 Fragments arranged by size: 1525 1456 1126 631 605 358 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 2807 4062 5218 1790 Size: 1017 1255 1156 2573 Fragments arranged by size: 2573 1255 1156 1017 BmgBI CAC'GTC Cuts at: 1318 2923 1318 Size: 1605 4396 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 3732 4690 Size: 416 53 83 399 24 969 1346 958 Cuts at: 4690 442 Size: 1753 Fragments arranged by size: 1753 1346 969 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 3221 4661 1239 Size: 1121 861 1440 2579 Fragments arranged by size: 2579 1440 1121 861 Bpu10I CC'TnA_GC Cuts at: 3152 3152 Size: 6001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4126 4991 5289 4126 Size: 865 298 4838 Fragments arranged by size: 4838 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4664 882 Size: 617 3165 2219 Fragments arranged by size: 3165 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2071 3792 493 Size: 711 867 1721 2702 Fragments arranged by size: 2702 1721 867 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2736 3808 4190 4825 Size: 53 83 186 936 1203 1072 382 635 Cuts at: 4825 275 Size: 1451 Fragments arranged by size: 1451 1203 1072 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4434 4832 5106 5253 5664 4434 Size: 398 274 147 411 4771 Fragments arranged by size: 4771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5776 Fragments arranged by size: 5776 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 1186 Size: 1255 4746 BseYI C'CCAG_C Cuts at: 2324 5204 5700 2324 Size: 2880 496 2625 Fragments arranged by size: 2880 2625 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 1163 Size: 573 498 4930 Fragments arranged by size: 4930 573 498 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3451 4212 5240 1540 Size: 275 186 1159 291 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 291 275 186 BsiHKAI G_wGCw'C Cuts at: 4062 4147 5218 4062 Size: 85 1071 4845 Fragments arranged by size: 4845 1071 85 BsmI GAATG_Cn' Cuts at: 2872 3512 3605 2872 Size: 640 93 5268 Fragments arranged by size: 5268 640 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2807 3320 4062 4147 5218 1391 Size: 399 1017 513 742 85 1071 2174 Fragments arranged by size: 2174 1071 1017 742 513 399 85 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2395 2601 3356 844 Size: 1551 206 755 3489 Fragments arranged by size: 3489 1551 755 206 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4496 4670 65 Size: 1183 3248 174 1396 Fragments arranged by size: 3248 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4645 4832 1700 Size: 110 2835 187 2869 Fragments arranged by size: 2869 2835 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6001 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 2689 1184 Size: 1505 4496 BstXI CCAn_nnnn'nTGG Cuts at: 2830 2830 Size: 6001 BstYI r'GATC_y Cuts at: 1972 3331 3698 4083 4100 5552 5638 5650 5996 Size: 1359 367 385 17 1452 86 12 346 Cuts at: 5996 1972 Size: 1977 Fragments arranged by size: 1977 1452 1359 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2425 2425 Size: 6001 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 2401 2536 513 Size: 552 939 12 385 135 3978 Fragments arranged by size: 3978 939 552 385 135 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 2097 2335 3261 3517 4311 4331 2097 Size: 238 926 256 794 20 3767 Fragments arranged by size: 3767 926 794 256 238 20 ClaI AT'CG_AT Cuts at: 1554 2268 1554 Size: 714 5287 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2492 5008 809 Size: 507 1176 2516 1802 Fragments arranged by size: 2516 1802 1176 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2623 3157 Size: 54 1425 50 275 18 219 574 534 Cuts at: 3157 3448 4337 5966 8 Size: 291 889 1629 43 Fragments arranged by size: 1629 1425 889 574 534 291 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 3448 1537 Size: 275 1345 291 4090 Fragments arranged by size: 4090 1345 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 3134 3267 3931 1353 Size: 987 794 133 664 3423 Fragments arranged by size: 3423 987 794 664 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2360 3221 4064 4661 5448 5716 Size: 393 416 312 861 843 597 787 268 Cuts at: 5716 1239 Size: 1524 Fragments arranged by size: 1524 861 843 787 597 416 393 312 268 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2030 Size: 6001 EcoO109I rG'GnC_Cy Cuts at: 3148 3163 3208 3241 3148 Size: 15 45 33 5908 Fragments arranged by size: 5908 45 33 15 EcoRI G'AATT_C Cuts at: 2396 3310 3688 2396 Size: 914 378 4709 Fragments arranged by size: 4709 914 378 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 2039 5148 1678 Size: 361 3109 2531 Fragments arranged by size: 3109 2531 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3010 3042 4642 4674 4716 Size: 32 135 32 1438 32 1600 32 42 Cuts at: 4716 4748 1373 Size: 32 2626 Fragments arranged by size: 2626 1600 1438 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2497 3345 3591 669 Size: 988 281 559 848 246 3079 Fragments arranged by size: 3079 988 848 559 281 246 HindIII A'AGCT_T Cuts at: 748 3284 748 Size: 2536 3465 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KpnI G_GTAC'C Cuts at: 2617 3326 2617 Size: 709 5292 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2625 10 Size: 54 1425 343 793 3386 Fragments arranged by size: 3386 1425 793 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2566 2828 2877 3959 4318 4477 518 Size: 648 1400 262 49 1082 359 159 2042 Fragments arranged by size: 2042 1400 1082 648 359 262 159 49 MspA1I CmG'CkG Cuts at: 1606 2308 2393 3052 3156 3417 4094 5242 5487 Size: 702 85 659 104 261 677 1148 245 Cuts at: 5487 1606 Size: 2120 Fragments arranged by size: 2120 1148 702 677 659 261 245 104 85 NcoI C'CATG_G Cuts at: 513 1065 2004 2401 513 Size: 552 939 397 4113 Fragments arranged by size: 4113 939 552 397 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 2283 3359 4904 1132 Size: 97 1054 1076 1545 2229 Fragments arranged by size: 2229 1545 1076 1054 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6001 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PmlI CAC'GTG Cuts at: 2071 2071 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3163 3163 Size: 6001 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PstI C_TGCA'G Cuts at: 830 2392 2798 2977 3353 830 Size: 1562 406 179 376 3478 Fragments arranged by size: 3478 1562 406 376 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2308 2393 3052 2308 Size: 85 659 5257 Fragments arranged by size: 5257 659 85 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SapI GCTCTTCn'nnn_ Cuts at: 3134 3134 Size: 6001 SbfI CC_TGCA'GG Cuts at: 2798 3353 2798 Size: 555 5446 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SfcI C'TryA_G Cuts at: 826 1045 2388 2794 2973 3349 4484 5165 5356 Size: 219 1343 406 179 376 1135 681 191 Cuts at: 5356 826 Size: 1471 Fragments arranged by size: 1471 1343 1135 681 406 376 219 191 179 SfiI GGCCn_nnn'nGGCC Cuts at: 3156 3156 Size: 6001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4105 5006 5268 820 Size: 197 926 2162 901 262 1553 Fragments arranged by size: 2162 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 2283 3359 1132 Size: 1151 1076 3774 Fragments arranged by size: 3774 1151 1076 SspI AAT'ATT Cuts at: 5 52 2155 3925 5 Size: 47 2103 1770 2081 Fragments arranged by size: 2103 2081 1770 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2004 2401 3440 513 Size: 552 939 397 1039 3074 Fragments arranged by size: 3074 1039 939 552 397 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4028 4045 4198 4383 4828 325 Size: 1255 2448 17 153 185 445 1498 Fragments arranged by size: 2448 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2955 4247 5959 Size: 274 80 33 51 1443 978 1292 1712 Cuts at: 5959 96 Size: 138 Fragments arranged by size: 1712 1443 1292 978 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5961 5993 5961 Size: 32 5969 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2543 2543 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 4130 4130 Size: 6001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI