Restriction map for
pFN21AB7970
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AflII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AvrII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI PstI
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 438 GGTACC G^GTACC
AclI 812 AACGTT AA^CGTT
AflIII 426 ACATGT A^C[GA][CT]GT
Alw44I 135 GTGCAC G^TGCAC
AlwNI 833 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1175 GGGCCC GGGCC^C
ApaLI 135 GTGCAC G^TGCAC
Asp718I 438 GGTACC G^GTACC
AvaII 805 GGTCC G^G[AT]CC
AviII 158 TGCGCA TGC^GCA
BalI 957 TGGCCA TGG^CCA
BamHI 600 GGATCC G^GATCC
BanI 438 GGTACC G^G[CT][GA]CC
BbsI 761 GAAGAC GAAGAC(2/6)
BpuAI 761 GAAGAC GAAGAC(2/6)
BseRI 699 CTCCTC GAGGAG(10/8)
BsmAI 81 GTCTC GTCTC(1/5)
BsmFI 806 GTCCC GGGAC(10/14)
BspLU11I 426 ACATGT A^CATGT
BstXI 1190 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 600 GGATCC [GA]^GATC[CT]
Cac8I 1173 GCGGGC GC[ACGT]^[ACGT]GC
CfoI 159 GCGC GCG^C
DpnII 601 GATC ^GATC
DraI 43 TTTAAA TTT^AAA
DraII 1175 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1015 CCATGG C^C[GA][CT]GG
EaeI 957 TGGCCA [CT]^GGCC[GA]
EarI 401 GAAGAG CTCTTC(1/4)
EcoNI 41 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1175 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1041 ATGCAT ATGCA^T
FauI 1173 GCGGG CCCGC(4/6)
FspI 158 TGCGCA TGC^GCA
HhaI 159 GCGC GCG^C
HinP1I 159 GCGC G^CGC
HincII 245 GTCAAC GT[CT]^[GA]AC
HindII 245 GTCAAC GT[CT]^[GA]AC
KpnI 438 GGTACC GGTAC^C
Ksp632I 401 GAAGAG CTCTTC(1/4)
MboI 601 GATC ^GATC
MflI 600 GGATCC [GA]^GATC[CT]
MluNI 957 TGGCCA TGG^CCA
MscI 957 TGGCCA TGG^CCA
MslI 1016 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1171 CAGCGG C[AC]G^C[GT]G
NcoI 1015 CCATGG C^CATGG
NdeII 601 GATC ^GATC
NsiI 1041 ATGCAT ATGCA^T
PshAI 1162 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 812 AACGTT AA^CGTT
SacI 607 GAGCTC GAGCT^C
SapI 401 GAAGAGC GCTCTTC(1/4)
Sau3AI 601 GATC ^GATC
SmlI 443 CTCAAG C^T[CT][GA]AG
TaqI 657 TCGA T^CGA
TthHB8I 657 TCGA T^CGA
XbaI 618 TCTAGA T^CTAGA
XcmI 1141 CCATCAGGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 600 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 558 GCGG CCGC(-3/-1)
1173 GCGG
AcsI 361 GAATTT [GA]^AATT[CT]
878 GAATTT
AlwI 601 GATCC GGATC(4/5)
600 GGATC
ApoI 361 GAATTT [GA]^AATT[CT]
878 GAATTT
AspHI 135 GTGCAC G[AT]GC[AT]^C
607 GAGCTC
BcnI 381 CCGGG CC^[GC]GG
511 CCCGG
BfaI 142 CTAG C^TAG
619 CTAG
BsiHKAI 135 GTGCAC G[AT]GC[AT]^C
607 GAGCTC
BsiYI 41 CCTTTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1010 CCTACCCATGG
BslI 41 CCTTTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1010 CCTACCCATGG
BsrDI 155 CATTGC GCAATG(2/0)
707 GCAATG
BsrI 314 CCAGT ACTGG(1/-1)
496 CCAGT
BstF5I 507 CATCC GGATG(2/0)
961 CATCC
EcoT14I 1015 CCATGG C^C[AT][AT]GG
1128 CCTTGG
FokI 507 CATCC GGATG(9/13)
961 CATCC
HapII 381 CCGG C^CGG
512 CCGG
HpaII 381 CCGG C^CGG
512 CCGG
MaeI 142 CTAG C^TAG
619 CTAG
MaeII 243 ACGT A^CGT
813 ACGT
MspI 381 CCGG C^CGG
512 CCGG
NciI 381 CCGGG CC^[GC]GG
511 CCCGG
PleI 748 GACTC GAGTC(4/5)
898 GAGTC
SspI 23 AATATT AAT^ATT
527 AATATT
StyI 1015 CCATGG C^C[AT][AT]GG
1128 CCTTGG
TfiI 634 GATTC G^A[AT]TC
922 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BanII BbvI BmyI
BsaJI Bsp1286I BssKI BstNI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MseI MvaI MwoI NlaIII NlaIV
NspI RsaI Sau96I ScrFI Tru9I
TseI Tsp45I Tsp509I TspRI