(Circular) (Six-base) MAPSORT of: pFN21AB7970.seq Check: 3430 from: 1 to: 5935 pFN21AB7970 5935 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3745 278 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3278 1937 Size: 1341 4594 Acc65I G'GTAC_C Cuts at: 2443 3256 2443 Size: 813 5122 AclI AA'CG_TT Cuts at: 1563 2818 4062 4435 1563 Size: 1255 1244 373 3063 Fragments arranged by size: 3063 1255 1244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2742 2912 3998 5382 5650 1632 Size: 1110 170 1086 1384 268 1917 Fragments arranged by size: 1917 1384 1110 1086 268 170 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5738 AflIII A'CryG_T Cuts at: 1203 2431 4834 5811 1203 Size: 1228 2403 977 1327 Fragments arranged by size: 2403 1327 1228 977 AgeI A'CCGG_T Cuts at: 4766 4766 Size: 5935 AhdI GACnn_n'nnGTC Cuts at: 4664 4664 Size: 5935 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5935 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5935 AlwNI CAG_nnn'CTG Cuts at: 2843 3678 5250 2843 Size: 835 1572 3528 Fragments arranged by size: 3528 1572 835 ApaI G_GGCC'C Cuts at: 3184 3184 Size: 5935 ApaLI G'TGCA_C Cuts at: 2140 3992 5148 2140 Size: 1852 1156 2927 Fragments arranged by size: 2927 1852 1156 ApoI r'AATT_y Cuts at: 1493 2366 2883 3244 3470 3622 5731 1493 Size: 873 517 361 226 152 2109 1697 Fragments arranged by size: 2109 1697 873 517 361 226 152 AseI AT'TA_AT Cuts at: 160 1032 4489 160 Size: 872 3457 1606 Fragments arranged by size: 3457 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 AvaI C'yCGr_G Cuts at: 1870 1943 3260 1870 Size: 73 1317 4545 Fragments arranged by size: 4545 1317 73 BamHI G'GATC_C Cuts at: 2605 3265 3632 2605 Size: 660 367 4908 Fragments arranged by size: 4908 660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2443 3256 4711 618 Size: 325 217 627 656 813 1455 1842 Fragments arranged by size: 1842 1455 813 656 627 325 217 BanII G_rGCy'C Cuts at: 1391 2598 2616 3184 3254 1391 Size: 1207 18 568 70 4072 Fragments arranged by size: 4072 1207 568 70 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2773 928 Size: 971 874 4090 Fragments arranged by size: 4090 971 874 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4126 4160 1903 Size: 34 50 34 2105 34 3678 Fragments arranged by size: 3678 2105 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3827 5037 3827 Size: 1210 4725 BclI T'GATC_A Cuts at: 1548 1548 Size: 5935 BfrBI ATG'CAT Cuts at: 3048 3048 Size: 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4546 136 Size: 107 122 71 631 605 2874 1525 Fragments arranged by size: 2874 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5930 5930 Size: 5935 BlpI GC'TnA_GC Cuts at: 3352 3352 Size: 5935 Bme1580I G_kGCm'C Cuts at: 1790 2144 3184 3996 5152 1790 Size: 354 1040 812 1156 2573 Fragments arranged by size: 2573 1156 1040 812 354 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5935 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 3666 4624 Size: 416 53 83 399 24 1078 1171 958 Cuts at: 4624 442 Size: 1753 Fragments arranged by size: 1753 1171 1078 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5935 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4595 1239 Size: 3356 2579 Bpu10I CC'TnA_GC Cuts at: 2610 2610 Size: 5935 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2433 4060 4925 5223 2433 Size: 1627 865 298 3145 Fragments arranged by size: 3145 1627 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4598 882 Size: 617 3099 2219 Fragments arranged by size: 3099 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3726 493 Size: 711 2522 2702 Fragments arranged by size: 2702 2522 711 BsaBI GATnn'nnATC Cuts at: 5929 5929 Size: 5935 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3742 4124 4759 275 Size: 53 83 186 936 2209 382 635 1451 Fragments arranged by size: 2209 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4368 4766 5040 5187 5598 4368 Size: 398 274 147 411 4705 Fragments arranged by size: 4705 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5710 Fragments arranged by size: 5710 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2695 1186 Size: 1509 4426 BseYI C'CCAG_C Cuts at: 5138 5634 5138 Size: 496 5439 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5362 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3385 4146 5174 1540 Size: 275 186 1384 761 1028 2301 Fragments arranged by size: 2301 1384 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 2616 3996 4081 5152 2144 Size: 472 1380 85 1071 2927 Fragments arranged by size: 2927 1380 1071 472 85 BsmI GAATG_Cn' Cuts at: 3446 3539 3446 Size: 93 5842 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2598 2616 3184 3254 3996 4081 Size: 399 354 454 18 568 70 742 85 Cuts at: 4081 5152 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 568 454 399 354 85 70 18 BspEI T'CCGG_A Cuts at: 5598 5598 Size: 5935 BspHI T'CATG_A Cuts at: 3824 5554 3824 Size: 1730 4205 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3290 844 Size: 2446 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3822 1394 Size: 429 1999 3507 Fragments arranged by size: 3507 1999 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2159 2719 4430 4604 65 Size: 1183 911 560 1711 174 1396 Fragments arranged by size: 1711 1396 1183 911 560 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4579 4766 1700 Size: 110 2769 187 2869 Fragments arranged by size: 2869 2769 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5935 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5935 BssSI C'ACGA_G Cuts at: 3995 5007 3995 Size: 1012 4923 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5935 BstBI TT'CG_AA Cuts at: 4739 4739 Size: 5935 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5935 BstXI CCAn_nnnn'nTGG Cuts at: 3202 3202 Size: 5935 BstYI r'GATC_y Cuts at: 1972 2605 3265 3632 4017 4034 5486 5572 5584 Size: 633 660 367 385 17 1452 86 12 Cuts at: 5584 5930 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 660 633 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3020 513 Size: 552 1955 3428 Fragments arranged by size: 3428 1955 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5196 Fragments arranged by size: 5196 683 56 BtsI GCAGTG_nn' Cuts at: 2173 2938 3032 3451 4245 4265 2173 Size: 765 94 419 794 20 3843 Fragments arranged by size: 3843 794 765 419 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5935 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5900 DraI TTT'AAA Cuts at: 2050 3239 2050 Size: 1189 4746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4942 809 Size: 507 3626 1802 Fragments arranged by size: 3626 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2962 3382 4271 Size: 54 1425 50 275 18 1132 420 889 Cuts at: 4271 5900 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1132 889 420 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3382 1537 Size: 275 1570 4090 Fragments arranged by size: 4090 1570 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2401 3865 1353 Size: 1048 1464 3423 Fragments arranged by size: 3423 1464 1048 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4522 4896 5042 387 Size: 1064 138 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2614 2614 Size: 5935 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2742 2912 3998 4595 5382 5650 1239 Size: 393 1110 170 1086 597 787 268 1524 Fragments arranged by size: 1524 1110 1086 787 597 393 268 170 EcoNI CCTnn'n_nnAGG Cuts at: 2050 2050 Size: 5935 EcoO109I rG'GnC_Cy Cuts at: 3181 3181 Size: 5935 EcoRI G'AATT_C Cuts at: 3244 3622 3244 Size: 378 5557 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2427 2427 Size: 5935 FspI TGC'GCA Cuts at: 2165 4441 2165 Size: 2276 3659 HaeII r_GCGC'y Cuts at: 1678 5082 1678 Size: 3404 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2690 2722 4576 4608 4650 Size: 32 135 32 1118 32 1854 32 42 Cuts at: 4650 4682 1373 Size: 32 2626 Fragments arranged by size: 2626 1854 1118 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2252 3279 3525 669 Size: 988 281 314 1027 246 3079 Fragments arranged by size: 3079 1027 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5935 HpaI GTT'AAC Cuts at: 3525 3525 Size: 5935 KpnI G_GTAC'C Cuts at: 2447 3260 2447 Size: 813 5122 MfeI C'AATT_G Cuts at: 3534 3534 Size: 5935 MluI A'CGCG_T Cuts at: 5811 5811 Size: 5935 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2931 5049 5233 1305 Size: 1626 2118 184 2007 Fragments arranged by size: 2118 2007 1626 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2964 10 Size: 54 1425 343 1132 2981 Fragments arranged by size: 2981 1425 1132 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3025 3893 4252 4411 518 Size: 648 1859 868 359 159 2042 Fragments arranged by size: 2042 1859 868 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3178 3351 4028 5176 5421 1606 Size: 1572 173 677 1148 245 2120 Fragments arranged by size: 2120 1572 1148 677 245 173 NcoI C'CATG_G Cuts at: 513 1065 3020 513 Size: 552 1955 3428 Fragments arranged by size: 3428 1955 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5935 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5935 NotI GC'GGCC_GC Cuts at: 3382 3382 Size: 5935 NsiI A_TGCA'T Cuts at: 3050 3050 Size: 5935 NspI r_CATG'y Cuts at: 1132 1229 2216 2435 3048 3293 4838 1132 Size: 97 987 219 613 245 1545 2229 Fragments arranged by size: 2229 1545 987 613 245 219 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5935 PciI A'CATG_T Cuts at: 2431 4834 2431 Size: 2403 3532 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5935 PfoI T'CCnGG_A Cuts at: 2526 2526 Size: 5935 PmeI GTTT'AAAC Cuts at: 3239 3239 Size: 5935 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4004 4036 5517 5549 4004 Size: 32 1481 32 4390 Fragments arranged by size: 4390 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3171 1613 Size: 1558 4377 PsiI TTA'TAA Cuts at: 2271 2538 3505 2271 Size: 267 967 4701 Fragments arranged by size: 4701 967 267 PspOMI G'GGCC_C Cuts at: 3180 3180 Size: 5935 PstI C_TGCA'G Cuts at: 830 3287 830 Size: 2457 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5935 SacI G_AGCT'C Cuts at: 2616 2616 Size: 5935 SalI G'TCGA_C Cuts at: 1936 3277 1936 Size: 1341 4594 SapI GCTCTTCn'nnn_ Cuts at: 2401 2401 Size: 5935 SbfI CC_TGCA'GG Cuts at: 3287 3287 Size: 5935 ScaI AGT'ACT Cuts at: 4183 5895 4183 Size: 1712 4223 SfcI C'TryA_G Cuts at: 826 1045 3283 4418 5099 5290 826 Size: 219 2238 1135 681 191 1471 Fragments arranged by size: 2238 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5935 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5935 SmaI CCC'GGG Cuts at: 1872 3262 1872 Size: 1390 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2448 4039 4940 5202 820 Size: 197 926 505 1591 901 262 1553 Fragments arranged by size: 1591 1553 926 901 505 262 197 SnaBI TAC'GTA Cuts at: 493 3726 493 Size: 3233 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5935 SphI G_CATG'C Cuts at: 1132 3293 1132 Size: 2161 3774 SspI AAT'ATT Cuts at: 5 52 2030 2534 3859 5 Size: 47 1978 504 1325 2081 Fragments arranged by size: 2081 1978 1325 504 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5935 StyI C'CwwG_G Cuts at: 513 1065 3020 3133 3374 513 Size: 552 1955 113 241 3074 Fragments arranged by size: 3074 1955 552 241 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 3208 3962 3979 4132 4317 4762 Size: 1255 444 1184 754 17 153 185 445 Cuts at: 4762 325 Size: 1498 Fragments arranged by size: 1498 1255 1184 754 445 444 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2457 2824 4181 Size: 274 80 33 51 1443 480 367 1357 Cuts at: 4181 5893 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1357 480 367 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 5895 5927 2421 Size: 32 3442 32 2429 Fragments arranged by size: 3442 2429 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5935 XbaI T'CTAG_A Cuts at: 2623 3271 2623 Size: 648 5287 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3153 3153 Size: 5935 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5935 XmaI C'CCGG_G Cuts at: 1870 3260 1870 Size: 1390 4545 XmnI GAAnn'nnTTC Cuts at: 4064 4064 Size: 5935 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3743 276 Size: 53 83 186 936 2209 2468 Fragments arranged by size: 2468 2209 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI