Restriction map for
pFN21AB7970
(Linear) (Six-base) MAPSORT of: KIBB7970.seq Check: 9757 from: 1 to: 1230
KIBB7970.orf
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
Acc65I G'GTAC_C
Cuts at: 0 438 1230
Size: 438 792
AclI AA'CG_TT
Cuts at: 0 813 1230
Size: 813 417
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 907 1230
Size: 737 170 323
Fragments arranged by size:
737 323 170
AflIII A'CryG_T
Cuts at: 0 426 1230
Size: 426 804
AlwNI CAG_nnn'CTG
Cuts at: 0 838 1230
Size: 838 392
ApaI G_GGCC'C
Cuts at: 0 1179 1230
Size: 1179 51
ApaLI G'TGCA_C
Cuts at: 0 135 1230
Size: 135 1095
ApoI r'AATT_y
Cuts at: 0 361 878 1230
Size: 361 517 352
Fragments arranged by size:
517 361 352
BamHI G'GATC_C
Cuts at: 0 600 1230
Size: 600 630
BanI G'GyrC_C
Cuts at: 0 438 1230
Size: 438 792
BanII G_rGCy'C
Cuts at: 0 593 611 1179 1230
Size: 593 18 568 51
Fragments arranged by size:
593 568 51 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 768 1230
Size: 768 462
BfrBI ATG'CAT
Cuts at: 0 1043 1230
Size: 1043 187
Bme1580I G_kGCm'C
Cuts at: 0 139 1179 1230
Size: 139 1040 51
Fragments arranged by size:
1040 139 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 1230
Size: 490 740
Bpu10I CC'TnA_GC
Cuts at: 0 605 1230
Size: 605 625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1230
Size: 428 802
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 690 1230
Size: 690 540
BsiHKAI G_wGCw'C
Cuts at: 0 139 611 1230
Size: 139 472 619
Fragments arranged by size:
619 472 139
Bsp1286I G_dGCh'C
Cuts at: 0 139 593 611 1179 1230
Size: 139 454 18 568 51
Fragments arranged by size:
568 454 139 51 18
BsrDI GCAATG_nn'
Cuts at: 0 154 714 1230
Size: 154 560 516
Fragments arranged by size:
560 516 154
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1197 1230
Size: 1197 33
BstYI r'GATC_y
Cuts at: 0 600 1230
Size: 600 630
BtgI C'CryG_G
Cuts at: 0 1015 1230
Size: 1015 215
BtsI GCAGTG_nn'
Cuts at: 0 168 933 1027 1230
Size: 168 765 94 203
Fragments arranged by size:
765 203 168 94
DraI TTT'AAA
Cuts at: 0 45 1230
Size: 45 1185
EaeI y'GGCC_r
Cuts at: 0 957 1230
Size: 957 273
EarI CTCTTCn'nnn_
Cuts at: 0 396 1230
Size: 396 834
EcoICRI GAG'CTC
Cuts at: 0 609 1230
Size: 609 621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 907 1230
Size: 737 170 323
Fragments arranged by size:
737 323 170
EcoNI CCTnn'n_nnAGG
Cuts at: 0 45 1230
Size: 45 1185
EcoO109I rG'GnC_Cy
Cuts at: 0 1176 1230
Size: 1176 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 422 1230
Size: 422 808
FspI TGC'GCA
Cuts at: 0 160 1230
Size: 160 1070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 1230
Size: 685 32 513
Fragments arranged by size:
685 513 32
HincII GTy'rAC
Cuts at: 0 247 1230
Size: 247 983
KpnI G_GTAC'C
Cuts at: 0 442 1230
Size: 442 788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1230
Size: 926 304
MscI TGG'CCA
Cuts at: 0 959 1230
Size: 959 271
MslI CAynn'nnrTG
Cuts at: 0 1020 1230
Size: 1020 210
MspA1I CmG'CkG
Cuts at: 0 1173 1230
Size: 1173 57
NcoI C'CATG_G
Cuts at: 0 1015 1230
Size: 1015 215
NsiI A_TGCA'T
Cuts at: 0 1045 1230
Size: 1045 185
NspI r_CATG'y
Cuts at: 0 211 430 1043 1230
Size: 211 219 613 187
Fragments arranged by size:
613 219 211 187
PciI A'CATG_T
Cuts at: 0 426 1230
Size: 426 804
PfoI T'CCnGG_A
Cuts at: 0 521 1230
Size: 521 709
PshAI GACnn'nnGTC
Cuts at: 0 1166 1230
Size: 1166 64
PsiI TTA'TAA
Cuts at: 0 266 533 1230
Size: 266 267 697
Fragments arranged by size:
697 267 266
PspOMI G'GGCC_C
Cuts at: 0 1175 1230
Size: 1175 55
SacI G_AGCT'C
Cuts at: 0 611 1230
Size: 611 619
SapI GCTCTTCn'nnn_
Cuts at: 0 396 1230
Size: 396 834
SmlI C'TyrA_G
Cuts at: 0 443 1230
Size: 443 787
SspI AAT'ATT
Cuts at: 0 25 529 1230
Size: 25 504 701
Fragments arranged by size:
701 504 25
StyI C'CwwG_G
Cuts at: 0 1015 1128 1230
Size: 1015 113 102
Fragments arranged by size:
1015 113 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 1203 1230
Size: 19 1184 27
Fragments arranged by size:
1184 27 19
TatI w'GTAC_w
Cuts at: 0 452 819 1230
Size: 452 367 411
Fragments arranged by size:
452 411 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 1230
Size: 416 32 782
Fragments arranged by size:
782 416 32
XbaI T'CTAG_A
Cuts at: 0 618 1230
Size: 618 612
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1148 1230
Size: 1148 82
Enzymes that do cut:
Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI ApoI
BamHI BanI BanII BbsI BfrBI Bme1580I BmrI Bpu10I
BpuEI BseRI BsiHKAI Bsp1286I BsrDI BstXI BstYI BtgI
BtsI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI Hin4I HincII KpnI MmeI MscI MslI
MspA1I NcoI NsiI NspI PciI PfoI PshAI PsiI
PspOMI SacI SapI SmlI SspI StyI TaqII TatI
TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AhdI AleI
AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
BmgBI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoRI EcoRV FseI FspAI
HaeII HindIII HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XhoI XmaI XmnI
ZraI