(Circular) (Six-base) MAPSORT of: pFN21AB7948.seq Check: 1296 from: 1 to: 5830 pFN21AB7948 5830 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2545 2545 Size: 5830 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2967 3173 1937 Size: 1030 206 4594 Fragments arranged by size: 4594 1030 206 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 3893 5277 5545 1632 Size: 434 1827 1384 268 1917 Fragments arranged by size: 1917 1827 1384 434 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 2806 4729 5706 1203 Size: 1603 1923 977 1327 Fragments arranged by size: 1923 1603 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 2192 3573 5145 2192 Size: 1381 1572 2877 Fragments arranged by size: 2877 1572 1381 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2165 2220 3139 3365 3517 5626 1493 Size: 672 55 919 226 152 2109 1697 Fragments arranged by size: 2109 1697 919 672 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2382 3155 1870 Size: 73 439 773 4545 Fragments arranged by size: 4545 773 439 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3151 4606 618 Size: 325 217 627 1364 1455 1842 Fragments arranged by size: 1842 1455 1364 627 325 217 BanII G_rGCy'C Cuts at: 1391 3149 1391 Size: 1758 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2019 928 Size: 971 120 4739 Fragments arranged by size: 4739 971 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 3045 3247 3045 Size: 202 5628 Bme1580I G_kGCm'C Cuts at: 1790 3891 5047 1790 Size: 2101 1156 2573 Fragments arranged by size: 2573 2101 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2257 3561 4519 Size: 416 53 83 399 24 840 1304 958 Cuts at: 4519 442 Size: 1753 Fragments arranged by size: 1753 1304 958 840 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4490 1239 Size: 3251 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3955 4820 5118 3955 Size: 865 298 4667 Fragments arranged by size: 4667 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 3621 493 Size: 711 837 1580 2702 Fragments arranged by size: 2702 1580 837 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5605 Fragments arranged by size: 5605 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3057 3075 1186 Size: 1871 18 3941 Fragments arranged by size: 3941 1871 18 BseYI C'CCAG_C Cuts at: 5033 5529 5033 Size: 496 5334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2523 2781 1163 Size: 573 787 258 4212 Fragments arranged by size: 4212 787 573 258 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3046 3891 3976 5047 3046 Size: 845 85 1071 3829 Fragments arranged by size: 3829 1071 845 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3046 3149 3891 3976 5047 1391 Size: 399 1256 103 742 85 1071 2174 Fragments arranged by size: 2174 1256 1071 742 399 103 85 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2545 3185 844 Size: 1701 640 3489 Fragments arranged by size: 3489 1701 640 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2149 4325 4499 65 Size: 1183 901 2176 174 1396 Fragments arranged by size: 2176 1396 1183 901 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 2192 2201 2462 2768 794 Size: 1398 9 261 306 3856 Fragments arranged by size: 3856 1398 306 261 9 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 2045 1184 Size: 861 4969 BstXI CCAn_nnnn'nTGG Cuts at: 2903 2903 Size: 5830 BstYI r'GATC_y Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825 Size: 1188 367 385 17 1452 86 12 346 Cuts at: 5825 1972 Size: 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2968 2968 Size: 5830 BtgI C'CryG_G Cuts at: 513 1065 2978 513 Size: 552 1913 3365 Fragments arranged by size: 3365 1913 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4140 4160 3346 Size: 794 20 5016 Fragments arranged by size: 5016 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795 Size: 54 1425 50 275 18 1447 889 1629 Cuts at: 5795 8 Size: 43 Fragments arranged by size: 1629 1447 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3760 1353 Size: 2407 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 3893 4490 5277 5545 1239 Size: 393 434 1827 597 787 268 1524 Fragments arranged by size: 1827 1524 787 597 434 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3054 3054 Size: 5830 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 EcoRV GAT'ATC Cuts at: 2341 2341 Size: 5830 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 4977 1678 Size: 3299 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 4471 4503 4545 Size: 32 135 32 758 32 2109 32 42 Cuts at: 4545 4577 1373 Size: 32 2626 Fragments arranged by size: 2626 2109 758 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2652 2869 3174 3420 669 Size: 988 281 714 217 305 246 3079 Fragments arranged by size: 3079 988 714 305 281 246 217 HindIII A'AGCT_T Cuts at: 748 748 Size: 5830 HpaI GTT'AAC Cuts at: 2652 3420 2652 Size: 768 5062 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 2515 3429 2515 Size: 914 4916 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2884 4944 5128 1305 Size: 1579 2060 184 2007 Fragments arranged by size: 2060 2007 1579 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2181 2295 2757 3788 4147 4306 518 Size: 648 1015 114 462 1031 359 159 2042 Fragments arranged by size: 2042 1031 1015 648 462 359 159 114 MspA1I CmG'CkG Cuts at: 1606 2189 3246 3923 5071 5316 1606 Size: 583 1057 677 1148 245 2120 Fragments arranged by size: 2120 1148 1057 677 583 245 NcoI C'CATG_G Cuts at: 513 1065 2978 513 Size: 552 1913 3365 Fragments arranged by size: 3365 1913 552 NdeI CA'TA_TG Cuts at: 387 2475 387 Size: 2088 3742 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2810 3188 4733 1132 Size: 97 1581 378 1545 2229 Fragments arranged by size: 2229 1581 1545 378 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 2806 4729 2806 Size: 1923 3907 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2069 2874 1613 Size: 456 805 4569 Fragments arranged by size: 4569 805 456 PsiI TTA'TAA Cuts at: 2442 3400 2442 Size: 958 4872 PstI C_TGCA'G Cuts at: 830 3182 830 Size: 2352 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2189 2189 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2872 3178 4313 4994 5185 826 Size: 219 1827 306 1135 681 191 1471 Fragments arranged by size: 1827 1471 1135 681 306 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 2384 3157 1872 Size: 512 773 4545 Fragments arranged by size: 4545 773 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 3934 4835 5097 820 Size: 197 926 1991 901 262 1553 Fragments arranged by size: 1991 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2041 3621 493 Size: 1548 1580 2702 Fragments arranged by size: 2702 1580 1548 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2978 3055 3269 513 Size: 552 1913 77 214 3074 Fragments arranged by size: 3074 1913 552 214 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2483 2931 3020 3857 3874 4027 4212 Size: 1255 903 448 89 837 17 153 185 Cuts at: 4212 4657 325 Size: 445 1498 Fragments arranged by size: 1498 1255 903 837 448 445 185 153 89 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2549 2618 2625 Size: 274 80 33 51 1443 572 69 7 Cuts at: 2625 4076 5788 96 Size: 1451 1712 138 Fragments arranged by size: 1712 1451 1443 572 274 138 80 69 51 33 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2303 3101 2303 Size: 798 5032 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 2382 3155 1870 Size: 512 773 4545 Fragments arranged by size: 4545 773 512 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI