(Circular) (Six-base) MAPSORT of: pFN21AB7910.seq Check: 2991 from: 1 to: 5695 pFN21AB7910 5695 bp With 182 enzymes: SgfI * April 15, 2010 09:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2727 2727 Size: 5695 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2443 2842 2899 3758 5142 5410 1632 Size: 811 399 57 859 1384 268 1917 Fragments arranged by size: 1917 1384 859 811 399 268 57 AfeI AGC'GCT Cuts at: 2696 2696 Size: 5695 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 2817 1166 Size: 1651 4044 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 2167 3438 5010 2167 Size: 1271 1572 2852 Fragments arranged by size: 2852 1572 1271 ApaI G_GGCC'C Cuts at: 2249 2249 Size: 5695 ApaLI G'TGCA_C Cuts at: 2929 3752 4908 2929 Size: 823 1156 3716 Fragments arranged by size: 3716 1156 823 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 3020 1870 Size: 73 1077 4545 Fragments arranged by size: 4545 1077 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2104 2236 2967 3016 4471 Size: 325 217 627 317 132 731 49 1455 Cuts at: 4471 618 Size: 1842 Fragments arranged by size: 1842 1455 731 627 325 317 217 132 49 BanII G_rGCy'C Cuts at: 1391 2249 2448 2538 2870 3014 1391 Size: 858 199 90 332 144 4072 Fragments arranged by size: 4072 858 332 199 144 90 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2509 928 Size: 971 610 4114 Fragments arranged by size: 4114 971 610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2587 2621 3886 3920 1903 Size: 34 50 34 566 34 1265 34 3678 Fragments arranged by size: 3678 1265 566 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2228 2418 4306 Size: 107 122 71 631 605 556 190 1888 Cuts at: 4306 136 Size: 1525 Fragments arranged by size: 1888 1525 631 605 556 190 122 107 71 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2109 2249 2436 2933 3756 4912 1790 Size: 319 140 187 497 823 1156 2573 Fragments arranged by size: 2573 1156 823 497 319 187 140 BmgBI CAC'GTC Cuts at: 1318 2641 1318 Size: 1323 4372 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2417 2645 4355 1239 Size: 983 195 228 1710 2579 Fragments arranged by size: 2579 1710 983 228 195 Bpu10I CC'TnA_GC Cuts at: 2141 2141 Size: 5695 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2975 3820 4685 4983 2975 Size: 845 865 298 3687 Fragments arranged by size: 3687 865 845 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2106 4358 882 Size: 617 607 2252 2219 Fragments arranged by size: 2252 2219 617 607 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 3884 4519 275 Size: 53 83 186 936 1969 382 635 1451 Fragments arranged by size: 1969 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4128 4526 4800 4947 5358 4128 Size: 398 274 147 411 4465 Fragments arranged by size: 4465 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2630 2660 1178 Size: 30 165 30 1227 30 4213 Fragments arranged by size: 4213 1227 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5695 BseYI C'CCAG_C Cuts at: 2435 4898 5394 2435 Size: 2463 496 2736 Fragments arranged by size: 2736 2463 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 1163 Size: 573 348 4774 Fragments arranged by size: 4774 573 348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3145 3906 4934 1540 Size: 275 186 1144 761 1028 2301 Fragments arranged by size: 2301 1144 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2403 2870 2933 3756 3841 4912 2403 Size: 467 63 823 85 1071 3186 Fragments arranged by size: 3186 1071 823 467 85 63 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2249 2403 2436 2448 2538 2870 Size: 399 319 140 154 33 12 90 332 Cuts at: 2870 2933 3014 3756 3841 4912 1391 Size: 63 81 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 332 319 154 140 90 85 81 63 33 12 BspEI T'CCGG_A Cuts at: 5358 5358 Size: 5695 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2727 2967 3050 844 Size: 1883 240 83 3489 Fragments arranged by size: 3489 1883 240 83 BsrBI CCG'CTC Cuts at: 1394 1823 2080 3582 1394 Size: 429 257 1502 3507 Fragments arranged by size: 3507 1502 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2361 4339 4526 1700 Size: 110 551 1978 187 2869 Fragments arranged by size: 2869 1978 551 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstYI r'GATC_y Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690 Size: 1053 367 385 17 1452 86 12 346 Cuts at: 5690 1972 Size: 1977 Fragments arranged by size: 1977 1452 1053 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 3211 4005 4025 3211 Size: 794 20 4881 Fragments arranged by size: 4881 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DraIII CAC_nnn'GTG Cuts at: 2500 2617 2500 Size: 117 5578 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2220 2337 2350 Size: 54 1425 50 275 18 390 117 13 Cuts at: 2350 2608 3142 4031 5660 8 Size: 258 534 889 1629 43 Fragments arranged by size: 1629 1425 889 534 390 275 258 117 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 3625 1353 Size: 668 1604 3423 Fragments arranged by size: 3423 1604 668 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4282 4656 4802 387 Size: 1064 138 2693 374 146 1280 Fragments arranged by size: 2693 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2868 2868 Size: 5695 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2417 2443 2645 2842 2899 3758 Size: 393 590 195 26 202 197 57 859 Cuts at: 3758 4355 5142 5410 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 859 787 597 590 393 268 202 197 195 57 26 EcoO109I rG'GnC_Cy Cuts at: 2245 2246 2368 2568 2245 Size: 1 122 200 5372 Fragments arranged by size: 5372 200 122 1 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2335 2335 Size: 5695 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2698 4842 1678 Size: 1020 2144 2531 Fragments arranged by size: 2531 2144 1020 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 4336 4368 4410 Size: 32 135 32 692 32 2040 32 42 Cuts at: 4410 4442 1373 Size: 32 2626 Fragments arranged by size: 2626 2040 692 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 2811 2895 4809 4993 1305 Size: 1011 495 84 1914 184 2007 Fragments arranged by size: 2007 1914 1011 495 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 2222 2339 2352 10 Size: 54 1425 343 390 117 13 3353 Fragments arranged by size: 3353 1425 390 343 117 54 13 MslI CAynn'nnrTG Cuts at: 518 1166 2817 3653 4012 4171 518 Size: 648 1651 836 359 159 2042 Fragments arranged by size: 2042 1651 836 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2919 3111 3788 4936 5181 1606 Size: 1313 192 677 1148 245 2120 Fragments arranged by size: 2120 1313 1148 677 245 192 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5143 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 2549 2677 3053 4598 1132 Size: 97 1320 128 376 1545 2229 Fragments arranged by size: 2229 1545 1320 376 128 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 2167 2218 1108 Size: 1059 51 4585 Fragments arranged by size: 4585 1059 51 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 5695 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2568 2568 Size: 5695 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2245 2245 Size: 5695 PstI C_TGCA'G Cuts at: 830 3047 830 Size: 2217 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2919 2919 Size: 5695 SacI G_AGCT'C Cuts at: 2870 2870 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SanDI GG'GwC_CC Cuts at: 2568 2568 Size: 5695 SapI GCTCTTCn'nnn_ Cuts at: 2021 2021 Size: 5695 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 3043 4178 4859 5050 826 Size: 219 1998 1135 681 191 1471 Fragments arranged by size: 1998 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2228 2228 Size: 5695 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 3022 1872 Size: 1150 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2990 3799 4700 4962 820 Size: 197 926 1047 809 901 262 1553 Fragments arranged by size: 1553 1047 926 901 809 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2687 2855 1517 Size: 1170 168 4357 Fragments arranged by size: 4357 1170 168 StyI C'CwwG_G Cuts at: 513 1065 2334 2451 3134 513 Size: 552 1269 117 683 3074 Fragments arranged by size: 3074 1269 683 552 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2729 2897 3722 3739 3892 4077 4522 Size: 1255 1149 168 825 17 153 185 445 Cuts at: 4522 325 Size: 1498 Fragments arranged by size: 1498 1255 1149 825 445 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3941 5653 96 Size: 274 80 33 51 1443 1964 1712 138 Fragments arranged by size: 1964 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 2630 2662 2768 2800 5655 5687 2001 Size: 32 597 32 106 32 2855 32 2009 Fragments arranged by size: 2855 2009 597 106 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 3020 1870 Size: 1150 4545 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI XcmI