(Circular) (Six-base) MAPSORT of: pFN21AB6128.seq Check: 1039 from: 1 to: 6586 pFN21AB6128 6586 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2354 2354 Size: 6586 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4396 278 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3929 1937 Size: 1992 4594 Acc65I G'GTAC_C Cuts at: 3907 3907 Size: 6586 AclI AA'CG_TT Cuts at: 1563 4713 5086 1563 Size: 3150 373 3063 Fragments arranged by size: 3150 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3179 3417 3551 4649 6033 6301 1632 Size: 1547 238 134 1098 1384 268 1917 Fragments arranged by size: 1917 1547 1384 1098 268 238 134 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6389 AflIII A'CryG_T Cuts at: 1203 5485 6462 1203 Size: 4282 977 1327 Fragments arranged by size: 4282 1327 977 AgeI A'CCGG_T Cuts at: 5417 5417 Size: 6586 AhdI GACnn_n'nnGTC Cuts at: 5315 5315 Size: 6586 AleI CACnn'nnGTG Cuts at: 1166 2936 3378 1166 Size: 1770 442 4374 Fragments arranged by size: 4374 1770 442 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3761 1184 Size: 2577 4009 AlwNI CAG_nnn'CTG Cuts at: 2746 3104 3151 4329 5901 2746 Size: 358 47 1178 1572 3431 Fragments arranged by size: 3431 1572 1178 358 47 ApaI G_GGCC'C Cuts at: 2800 2800 Size: 6586 ApaLI G'TGCA_C Cuts at: 4643 5799 4643 Size: 1156 5430 ApoI r'AATT_y Cuts at: 1493 2403 3895 4121 4273 6382 1493 Size: 910 1492 226 152 2109 1697 Fragments arranged by size: 2109 1697 1492 910 226 152 AseI AT'TA_AT Cuts at: 160 1032 5140 160 Size: 872 4108 1606 Fragments arranged by size: 4108 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 AvaI C'yCGr_G Cuts at: 1870 1943 2811 3005 3764 3911 1870 Size: 73 868 194 759 147 4545 Fragments arranged by size: 4545 868 759 194 147 73 AvrII C'CTAG_G Cuts at: 3865 3865 Size: 6586 BamHI G'GATC_C Cuts at: 3916 4283 3916 Size: 367 6219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2590 3737 3907 5362 618 Size: 325 217 627 803 1147 170 1455 1842 Fragments arranged by size: 1842 1455 1147 803 627 325 217 170 BanII G_rGCy'C Cuts at: 1391 2156 2477 2734 2800 2876 2919 3006 3841 Size: 765 321 257 66 76 43 87 835 Cuts at: 3841 3905 1391 Size: 64 4072 Fragments arranged by size: 4072 835 765 321 257 87 76 66 64 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3077 3803 928 Size: 971 1178 726 3711 Fragments arranged by size: 3711 1178 971 726 BbvCI CC'TCA_GC Cuts at: 2162 2595 3803 2162 Size: 433 1208 4945 Fragments arranged by size: 4945 1208 433 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2494 2528 2548 2582 2615 2649 3176 Size: 34 557 34 20 34 33 34 527 Cuts at: 3176 3210 4777 4811 1903 Size: 34 1567 34 3678 Fragments arranged by size: 3678 1567 557 527 34 34 34 34 34 34 33 20 BciVI GTATCCnnnnn_n' Cuts at: 4478 5688 4478 Size: 1210 5376 BclI T'GATC_A Cuts at: 1548 2136 1548 Size: 588 5998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2054 3867 Size: 107 122 71 631 605 377 5 1813 Cuts at: 3867 5197 136 Size: 1330 1525 Fragments arranged by size: 1813 1525 1330 631 605 377 122 107 71 5 BglII A'GATC_T Cuts at: 3543 6581 3543 Size: 3038 3548 BlpI GC'TnA_GC Cuts at: 2319 2733 4003 2319 Size: 414 1270 4902 Fragments arranged by size: 4902 1270 414 Bme1580I G_kGCm'C Cuts at: 1790 2800 3238 4647 5803 1790 Size: 1010 438 1409 1156 2573 Fragments arranged by size: 2573 1409 1156 1010 438 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6586 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2274 2695 4317 Size: 416 53 83 399 24 857 421 1622 Cuts at: 4317 5275 442 Size: 958 1753 Fragments arranged by size: 1753 1622 958 857 421 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6586 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2235 2235 Size: 6586 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2049 2453 2468 2933 3467 3518 3833 5246 Size: 810 404 15 465 534 51 315 1413 Cuts at: 5246 1239 Size: 2579 Fragments arranged by size: 2579 1413 810 534 465 404 315 51 15 Bpu10I CC'TnA_GC Cuts at: 2162 2220 2595 2991 3803 2162 Size: 58 375 396 812 4945 Fragments arranged by size: 4945 812 396 375 58 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4711 5576 5874 4711 Size: 865 298 5423 Fragments arranged by size: 5423 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2292 3753 5249 882 Size: 617 793 1461 1496 2219 Fragments arranged by size: 2219 1496 1461 793 617 BsaAI yAC'GTr Cuts at: 493 1204 2982 4377 493 Size: 711 1778 1395 2702 Fragments arranged by size: 2702 1778 1395 711 BsaBI GATnn'nnATC Cuts at: 6580 6580 Size: 6586 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3081 4393 4775 5410 Size: 53 83 186 936 1548 1312 382 635 Cuts at: 5410 275 Size: 1451 Fragments arranged by size: 1548 1451 1312 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2694 3516 3603 5019 5417 5691 5838 6249 2694 Size: 822 87 1416 398 274 147 411 3031 Fragments arranged by size: 3031 1416 822 411 398 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2848 2878 2993 3023 1178 Size: 30 165 30 1445 30 115 30 4741 Fragments arranged by size: 4741 1445 165 115 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2372 2612 3065 3278 3431 3782 1186 Size: 838 348 240 453 213 153 351 3990 Fragments arranged by size: 3990 838 453 351 348 240 213 153 BseYI C'CCAG_C Cuts at: 2781 2846 3237 3486 3787 5789 6285 2781 Size: 65 391 249 301 2002 496 3082 Fragments arranged by size: 3082 2002 496 391 301 249 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2093 3113 3356 3677 1163 Size: 573 357 1020 243 321 4072 Fragments arranged by size: 4072 1020 573 357 321 243 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4036 4797 5825 1540 Size: 275 186 2035 761 1028 2301 Fragments arranged by size: 2301 2035 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2866 3006 4647 4732 5803 2866 Size: 140 1641 85 1071 3649 Fragments arranged by size: 3649 1641 1071 140 85 BsmI GAATG_Cn' Cuts at: 4097 4190 4097 Size: 93 6493 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2477 2734 2800 2866 2876 2919 Size: 399 366 321 257 66 66 10 43 Cuts at: 2919 3006 3238 3841 3905 4647 4732 5803 1391 Size: 87 232 603 64 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 399 366 321 257 232 87 85 66 66 64 43 10 BspEI T'CCGG_A Cuts at: 3516 6249 3516 Size: 2733 3853 BspHI T'CATG_A Cuts at: 4475 6205 4475 Size: 1730 4856 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 2354 3941 844 Size: 1466 44 1587 3489 Fragments arranged by size: 3489 1587 1466 44 BsrBI CCG'CTC Cuts at: 1394 1823 4473 1394 Size: 429 2650 3507 Fragments arranged by size: 3507 2650 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5081 5255 65 Size: 1183 3833 174 1396 Fragments arranged by size: 3833 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2048 2667 3111 5230 5417 1700 Size: 110 238 619 444 2119 187 2869 Fragments arranged by size: 2869 2119 619 444 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6586 BssHII G'CGCG_C Cuts at: 1925 2083 1925 Size: 158 6428 BssSI C'ACGA_G Cuts at: 3695 4646 5658 3695 Size: 951 1012 4623 Fragments arranged by size: 4623 1012 951 BstAPI GCAn_nnn'nTGC Cuts at: 794 2080 2746 3151 794 Size: 1286 666 405 4229 Fragments arranged by size: 4229 1286 666 405 BstBI TT'CG_AA Cuts at: 5390 5390 Size: 6586 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6586 BstXI CCAn_nnnn'nTGG Cuts at: 2101 2215 2509 2101 Size: 114 294 6178 Fragments arranged by size: 6178 294 114 BstYI r'GATC_y Cuts at: 1972 3360 3543 3819 3916 4283 4668 4685 6137 Size: 1388 183 276 97 367 385 17 1452 Cuts at: 6137 6223 6235 6581 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1388 385 367 346 276 183 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2868 2868 Size: 6586 BtgI C'CryG_G Cuts at: 513 1065 2004 2543 513 Size: 552 939 539 4556 Fragments arranged by size: 4556 939 552 539 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5847 Fragments arranged by size: 5847 683 56 BtsI GCAGTG_nn' Cuts at: 2420 4102 4896 4916 2420 Size: 1682 794 20 4090 Fragments arranged by size: 4090 1682 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6586 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6551 DraI TTT'AAA Cuts at: 3890 3890 Size: 6586 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5593 809 Size: 507 4277 1802 Fragments arranged by size: 4277 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2205 2669 4033 Size: 54 1425 50 275 18 375 464 1364 Cuts at: 4033 4922 6551 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1364 889 464 375 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4033 1537 Size: 275 2221 4090 Fragments arranged by size: 4090 2221 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2315 3155 3393 3525 4516 1353 Size: 962 840 238 132 991 3423 Fragments arranged by size: 3423 991 962 840 238 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2030 2189 3833 5173 5547 5693 Size: 1064 138 441 159 1644 1340 374 146 Cuts at: 5693 387 Size: 1280 Fragments arranged by size: 1644 1340 1280 1064 441 374 159 146 138 EcoICRI GAG'CTC Cuts at: 3004 3004 Size: 6586 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2453 2468 2933 3179 3417 3467 Size: 393 417 404 15 465 246 238 50 Cuts at: 3467 3518 3551 3833 4649 5246 6033 6301 1239 Size: 51 33 282 816 597 787 268 1524 Fragments arranged by size: 1524 816 787 597 465 417 404 393 282 268 246 238 51 50 33 15 EcoNI CCTnn'n_nnAGG Cuts at: 2219 3845 2219 Size: 1626 4960 EcoO109I rG'GnC_Cy Cuts at: 2431 2785 2796 2797 2815 3148 3280 2431 Size: 354 11 1 18 333 132 5737 Fragments arranged by size: 5737 354 333 132 18 11 1 EcoRI G'AATT_C Cuts at: 3895 4273 3895 Size: 378 6208 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2920 2920 Size: 6586 FspI TGC'GCA Cuts at: 5092 5092 Size: 6586 HaeII r_GCGC'y Cuts at: 1678 5733 1678 Size: 4055 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2752 2784 2848 2880 2993 Size: 32 135 32 1180 32 64 32 113 Cuts at: 2993 3025 3323 3355 5227 5259 5301 5333 1373 Size: 32 298 32 1872 32 42 32 2626 Fragments arranged by size: 2626 1872 1180 298 135 113 64 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3930 4176 669 Size: 988 281 1992 246 3079 Fragments arranged by size: 3079 1992 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6586 HpaI GTT'AAC Cuts at: 4176 4176 Size: 6586 KpnI G_GTAC'C Cuts at: 3911 3911 Size: 6586 MfeI C'AATT_G Cuts at: 4185 4185 Size: 6586 MluI A'CGCG_T Cuts at: 6462 6462 Size: 6586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2584 5700 5884 1305 Size: 1279 3116 184 2007 Fragments arranged by size: 3116 2007 1279 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2207 10 Size: 54 1425 343 375 4389 Fragments arranged by size: 4389 1425 375 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 3378 3743 4544 4903 5062 518 Size: 648 1770 442 365 801 359 159 2042 Fragments arranged by size: 2042 1770 801 648 442 365 359 159 MspA1I CmG'CkG Cuts at: 1606 2124 2416 2737 2743 3025 3241 3433 4002 Size: 518 292 321 6 282 216 192 569 Cuts at: 4002 4679 5827 6072 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 569 518 321 292 282 245 216 192 6 NaeI GCC'GGC Cuts at: 2050 3113 2050 Size: 1063 5523 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5095 Fragments arranged by size: 5095 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6586 NgoMIV G'CCGG_C Cuts at: 2048 3111 2048 Size: 1063 5523 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6586 NotI GC'GGCC_GC Cuts at: 4033 4033 Size: 6586 NspI r_CATG'y Cuts at: 1132 1229 3944 5489 1132 Size: 97 2715 1545 2229 Fragments arranged by size: 2715 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3076 1348 Size: 1728 4858 PciI A'CATG_T Cuts at: 5485 5485 Size: 6586 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6586 PfoI T'CCnGG_A Cuts at: 3495 3822 3876 3495 Size: 327 54 6205 Fragments arranged by size: 6205 327 54 PmeI GTTT'AAAC Cuts at: 3890 3890 Size: 6586 PmlI CAC'GTG Cuts at: 2982 2982 Size: 6586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2988 3020 3617 3649 4655 4687 6168 6200 2988 Size: 32 597 32 1006 32 1481 32 3374 Fragments arranged by size: 3374 1481 1006 597 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2815 3280 2431 Size: 384 465 5737 Fragments arranged by size: 5737 465 384 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6586 PsiI TTA'TAA Cuts at: 4156 4156 Size: 6586 PspOMI G'GGCC_C Cuts at: 2796 2796 Size: 6586 PspXI vC'TCGA_Gb Cuts at: 3005 3764 3005 Size: 759 5827 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2258 2290 2258 Size: 32 6554 PstI C_TGCA'G Cuts at: 830 2365 2463 2643 2751 2835 3216 3321 3707 Size: 1535 98 180 108 84 381 105 386 Cuts at: 3707 3938 830 Size: 231 3478 Fragments arranged by size: 3478 1535 386 381 231 180 108 105 98 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6586 PvuII CAG'CTG Cuts at: 2416 3025 3433 2416 Size: 609 408 5569 Fragments arranged by size: 5569 609 408 RsrII CG'GwC_CG Cuts at: 2017 2017 Size: 6586 SacI G_AGCT'C Cuts at: 3006 3006 Size: 6586 SalI G'TCGA_C Cuts at: 1936 3928 1936 Size: 1992 4594 SanDI GG'GwC_CC Cuts at: 2431 3280 2431 Size: 849 5737 SapI GCTCTTCn'nnn_ Cuts at: 3155 3155 Size: 6586 SbfI CC_TGCA'GG Cuts at: 2365 3938 2365 Size: 1573 5013 ScaI AGT'ACT Cuts at: 4834 6546 4834 Size: 1712 4874 SfcI C'TryA_G Cuts at: 826 1045 2361 2459 2639 2747 2831 3212 3317 Size: 219 1316 98 180 108 84 381 105 Cuts at: 3317 3703 3934 5069 5750 5941 826 Size: 386 231 1135 681 191 1471 Fragments arranged by size: 1471 1316 1135 681 386 381 231 219 191 180 108 105 98 84 SfiI GGCCn_nnn'nGGCC Cuts at: 3867 3867 Size: 6586 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6586 SmaI CCC'GGG Cuts at: 1872 2813 3913 1872 Size: 941 1100 4545 Fragments arranged by size: 4545 1100 941 SmlI C'TyrA_G Cuts at: 820 1017 1943 3005 3764 4690 5591 5853 820 Size: 197 926 1062 759 926 901 262 1553 Fragments arranged by size: 1553 1062 926 926 901 759 262 197 SnaBI TAC'GTA Cuts at: 493 4377 493 Size: 3884 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6586 SphI G_CATG'C Cuts at: 1132 3944 1132 Size: 2812 3774 SspI AAT'ATT Cuts at: 5 52 4510 5 Size: 47 4458 2081 Fragments arranged by size: 4458 2081 47 StuI AGG'CCT Cuts at: 1517 2225 2837 3671 1517 Size: 708 612 834 4432 Fragments arranged by size: 4432 834 708 612 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 2426 3865 4025 513 Size: 552 939 243 179 1439 160 3074 Fragments arranged by size: 3074 1439 939 552 243 179 160 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2034 3500 4613 4630 4783 4968 5413 Size: 1255 454 1466 1113 17 153 185 445 Cuts at: 5413 325 Size: 1498 Fragments arranged by size: 1498 1466 1255 1113 454 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3128 4832 6544 Size: 274 80 33 51 1443 1151 1704 1712 Cuts at: 6544 96 Size: 138 Fragments arranged by size: 1712 1704 1443 1151 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2044 2076 2535 2567 2684 2716 6546 6578 2044 Size: 32 459 32 117 32 3830 32 2052 Fragments arranged by size: 3830 2052 459 117 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2687 1316 Size: 1371 5215 XbaI T'CTAG_A Cuts at: 3922 3922 Size: 6586 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3854 3854 Size: 6586 XhoI C'TCGA_G Cuts at: 1943 3005 3764 1943 Size: 1062 759 4765 Fragments arranged by size: 4765 1062 759 XmaI C'CCGG_G Cuts at: 1870 2811 3911 1870 Size: 941 1100 4545 Fragments arranged by size: 4545 1100 941 XmnI GAAnn'nnTTC Cuts at: 2492 4715 2492 Size: 2223 4363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4394 276 Size: 53 83 186 936 2860 2468 Fragments arranged by size: 2860 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI SacII SexAI SfoI SrfI SwaI