(Circular) (Six-base) MAPSORT of: pFN21AB7662.seq Check: 4036 from: 1 to: 5893 pFN21AB7662 5893 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2506 2506 Size: 5893 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2284 3236 1937 Size: 347 952 4594 Fragments arranged by size: 4594 952 347 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 3956 5340 5608 1632 Size: 477 1847 1384 268 1917 Fragments arranged by size: 1917 1847 1384 477 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AlwNI CAG_nnn'CTG Cuts at: 2321 3636 5208 2321 Size: 1315 1572 3006 Fragments arranged by size: 3006 1572 1315 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 2527 2660 2702 2920 3202 3428 3580 5689 Size: 1034 133 42 218 282 226 152 2109 Cuts at: 5689 1493 Size: 1697 Fragments arranged by size: 2109 1697 1034 282 226 218 152 133 42 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 2511 3218 1870 Size: 73 568 707 4545 Fragments arranged by size: 4545 707 568 73 BamHI G'GATC_C Cuts at: 2010 3223 3590 2010 Size: 1213 367 4313 Fragments arranged by size: 4313 1213 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 3214 4669 618 Size: 325 217 627 567 860 1455 1842 Fragments arranged by size: 1842 1455 860 627 567 325 217 BanII G_rGCy'C Cuts at: 1391 2136 2520 3212 1391 Size: 745 384 692 4072 Fragments arranged by size: 4072 745 692 384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2499 928 Size: 971 600 4322 Fragments arranged by size: 4322 971 600 BbvCI CC'TCA_GC Cuts at: 2039 2039 Size: 5893 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4084 4118 1903 Size: 34 2147 34 3678 Fragments arranged by size: 3678 2147 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 3310 3310 Size: 5893 Bme1580I G_kGCm'C Cuts at: 1790 2614 3954 5110 1790 Size: 824 1340 1156 2573 Fragments arranged by size: 2573 1340 1156 824 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2009 3624 4582 Size: 416 53 83 399 24 592 1615 958 Cuts at: 4582 442 Size: 1753 Fragments arranged by size: 1753 1615 958 592 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 4553 1239 Size: 1085 2229 2579 Fragments arranged by size: 2579 2229 1085 Bpu10I CC'TnA_GC Cuts at: 2039 2039 Size: 5893 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3090 4018 4883 5181 3090 Size: 928 865 298 3802 Fragments arranged by size: 3802 928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2243 4556 882 Size: 617 744 2313 2219 Fragments arranged by size: 2313 2219 744 617 BsaAI yAC'GTr Cuts at: 493 1204 2227 3684 493 Size: 711 1023 1457 2702 Fragments arranged by size: 2702 1457 1023 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2195 3700 4082 4717 Size: 53 83 186 936 662 1505 382 635 Cuts at: 4717 275 Size: 1451 Fragments arranged by size: 1505 1451 936 662 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2167 4326 4724 4998 5145 5556 2167 Size: 2159 398 274 147 411 2504 Fragments arranged by size: 2504 2159 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2792 2822 1178 Size: 30 165 30 1389 30 4249 Fragments arranged by size: 4249 1389 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2134 1186 Size: 948 4945 BseYI C'CCAG_C Cuts at: 5096 5592 5096 Size: 496 5397 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2777 1163 Size: 573 1041 4279 Fragments arranged by size: 4279 1041 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2236 3343 4104 5132 1540 Size: 275 186 235 1107 761 1028 2301 Fragments arranged by size: 2301 1107 1028 761 275 235 186 BsiHKAI G_wGCw'C Cuts at: 3954 4039 5110 3954 Size: 85 1071 4737 Fragments arranged by size: 4737 1071 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2520 2614 3212 3954 4039 5110 Size: 399 346 384 94 598 742 85 1071 Cuts at: 5110 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 598 399 384 346 94 85 BspEI T'CCGG_A Cuts at: 5556 5556 Size: 5893 BspHI T'CATG_A Cuts at: 3782 5512 3782 Size: 1730 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2506 3248 844 Size: 1662 742 3489 Fragments arranged by size: 3489 1662 742 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4388 4562 65 Size: 1183 3140 174 1396 Fragments arranged by size: 3140 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2113 4537 4724 1700 Size: 110 303 2424 187 2869 Fragments arranged by size: 2869 2424 303 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstYI r'GATC_y Cuts at: 1972 2010 2467 2722 3223 3590 3975 3992 5444 Size: 38 457 255 501 367 385 17 1452 Cuts at: 5444 5530 5542 5888 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 501 457 385 367 346 255 86 38 17 12 Bsu36I CC'TnA_GG Cuts at: 2410 2410 Size: 5893 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 3409 4203 4223 3409 Size: 794 20 5079 Fragments arranged by size: 5079 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 2461 2571 3197 2461 Size: 110 626 5157 Fragments arranged by size: 5157 626 110 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858 Size: 54 1425 50 275 18 1510 889 1629 Cuts at: 5858 8 Size: 43 Fragments arranged by size: 1629 1510 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3823 1353 Size: 2470 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2038 2172 4480 4854 5000 387 Size: 1064 138 449 134 2308 374 146 1280 Fragments arranged by size: 2308 1280 1064 449 374 146 138 134 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2324 3956 4553 5340 5608 1239 Size: 393 477 215 1632 597 787 268 1524 Fragments arranged by size: 1632 1524 787 597 477 393 268 215 EcoNI CCTnn'n_nnAGG Cuts at: 2333 2333 Size: 5893 EcoO109I rG'GnC_Cy Cuts at: 2318 2318 Size: 5893 EcoRI G'AATT_C Cuts at: 2702 3202 3580 2702 Size: 500 378 5015 Fragments arranged by size: 5015 500 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2224 2224 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 2064 3003 5040 1678 Size: 386 939 2037 2531 Fragments arranged by size: 2531 2037 939 386 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2792 2824 2899 2931 4534 Size: 32 135 32 1220 32 75 32 1603 Cuts at: 4534 4566 4608 4640 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1603 1220 135 75 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3094 3237 3483 669 Size: 988 281 1156 143 246 3079 Fragments arranged by size: 3079 1156 988 281 246 143 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3094 3483 3094 Size: 389 5504 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2560 5007 5191 1305 Size: 1255 2447 184 2007 Fragments arranged by size: 2447 2007 1255 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3851 4210 4369 518 Size: 648 2685 359 159 2042 Fragments arranged by size: 2685 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2155 2842 3309 3986 5134 5379 1606 Size: 549 687 467 677 1148 245 2120 Fragments arranged by size: 2120 1148 687 677 549 467 245 NaeI GCC'GGC Cuts at: 2115 2115 Size: 5893 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 2629 387 Size: 2242 3651 NgoMIV G'CCGG_C Cuts at: 2113 2113 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 3100 3251 4796 1132 Size: 97 1871 151 1545 2229 Fragments arranged by size: 2229 1871 1545 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 2321 1108 Size: 1213 4680 PfoI T'CCnGG_A Cuts at: 2320 2725 2320 Size: 405 5488 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2318 2318 Size: 5893 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3159 3463 3159 Size: 304 5589 PstI C_TGCA'G Cuts at: 830 3245 830 Size: 2415 3478 PvuI CG_AT'CG Cuts at: 2001 2236 2001 Size: 235 5658 PvuII CAG'CTG Cuts at: 2155 2155 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 3132 3241 4376 5057 5248 826 Size: 219 2087 109 1135 681 191 1471 Fragments arranged by size: 2087 1471 1135 681 219 191 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2511 3105 3997 4898 5160 820 Size: 197 926 568 594 892 901 262 1553 Fragments arranged by size: 1553 926 901 892 594 568 262 197 SnaBI TAC'GTA Cuts at: 493 2227 3684 493 Size: 1734 1457 2702 Fragments arranged by size: 2702 1734 1457 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5893 StyI C'CwwG_G Cuts at: 513 1065 2052 2186 2549 2804 3332 513 Size: 552 987 134 363 255 528 3074 Fragments arranged by size: 3074 987 552 528 363 255 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3920 3937 4090 4275 4720 325 Size: 1255 2340 17 153 185 445 1498 Fragments arranged by size: 2340 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2645 4139 5851 Size: 274 80 33 51 1443 668 1494 1712 Cuts at: 5851 96 Size: 138 Fragments arranged by size: 1712 1494 1443 668 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2113 2145 5853 5885 2113 Size: 32 3708 32 2121 Fragments arranged by size: 3708 2121 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 2511 1943 Size: 568 5325 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 2544 4022 2544 Size: 1478 4415 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI