(Circular) (Six-base) MAPSORT of: pFN21AB7657.seq Check: 8252 from: 1 to: 5707 pFN21AB7657 5707 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2365 2365 Size: 5707 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2246 2619 3770 5154 5422 1632 Size: 614 373 1151 1384 268 1917 Fragments arranged by size: 1917 1384 1151 614 373 268 AfeI AGC'GCT Cuts at: 2080 2080 Size: 5707 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 2060 2401 4538 2060 Size: 341 2137 3229 Fragments arranged by size: 3229 2137 341 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2687 3450 5022 2687 Size: 763 1572 3372 Fragments arranged by size: 3372 1572 763 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 3016 3242 3394 5503 1493 Size: 1523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2157 2226 2672 3032 1870 Size: 73 214 69 446 360 4545 Fragments arranged by size: 4545 446 360 214 73 69 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2274 2866 2965 3028 4483 Size: 325 217 627 487 592 99 63 1455 Cuts at: 4483 618 Size: 1842 Fragments arranged by size: 1842 1455 627 592 487 325 217 99 63 BanII G_rGCy'C Cuts at: 1391 2679 3026 1391 Size: 1288 347 4072 Fragments arranged by size: 4072 1288 347 BbeI G_GCGC'C Cuts at: 2278 2278 Size: 5707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2188 2493 2610 928 Size: 971 289 305 117 4025 Fragments arranged by size: 4025 971 305 289 117 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2231 4318 136 Size: 107 122 71 631 605 559 2087 1525 Fragments arranged by size: 2087 1525 631 605 559 122 107 71 BglII A'GATC_T Cuts at: 2628 5702 2628 Size: 3074 2633 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 2970 3768 4924 1790 Size: 1180 798 1156 2573 Fragments arranged by size: 2573 1180 1156 798 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2186 2496 2838 2993 4367 1239 Size: 856 91 310 342 155 1374 2579 Fragments arranged by size: 2579 1374 856 342 310 155 91 Bpu10I CC'TnA_GC Cuts at: 2465 2465 Size: 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2195 3832 4697 4995 2195 Size: 1637 865 298 2907 Fragments arranged by size: 2907 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2870 4370 882 Size: 617 1371 1500 2219 Fragments arranged by size: 2219 1500 1371 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2275 3514 3896 Size: 53 83 186 936 609 133 1239 382 Cuts at: 3896 4531 275 Size: 635 1451 Fragments arranged by size: 1451 1239 936 635 609 382 186 133 83 53 BsaWI w'CCGG_w Cuts at: 2060 2401 4140 4538 4812 4959 5370 2060 Size: 341 1739 398 274 147 411 2397 Fragments arranged by size: 2397 1739 411 398 341 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2744 2888 1186 Size: 1558 144 4005 Fragments arranged by size: 4005 1558 144 BseYI C'CCAG_C Cuts at: 2293 2802 2884 2936 4910 5406 2293 Size: 509 82 52 1974 496 2594 Fragments arranged by size: 2594 1974 509 496 82 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2399 2508 2734 1163 Size: 573 663 109 226 4136 Fragments arranged by size: 4136 663 573 226 109 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2382 2679 2907 3768 3853 4924 2382 Size: 297 228 861 85 1071 3165 Fragments arranged by size: 3165 1071 861 297 228 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 BsmBI CGTCTCn'nnnn_ Cuts at: 2771 2771 Size: 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2679 2907 2970 3026 3768 3853 Size: 399 592 297 228 63 56 742 85 Cuts at: 3853 4924 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 592 399 297 228 85 63 56 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2365 2718 3062 844 Size: 1521 353 344 3489 Fragments arranged by size: 3489 1521 353 344 BsrBI CCG'CTC Cuts at: 1394 1823 2030 3594 1394 Size: 429 207 1564 3507 Fragments arranged by size: 3507 1564 429 207 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2401 4351 4538 1700 Size: 110 250 341 1950 187 2869 Fragments arranged by size: 2869 1950 341 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 2039 1925 Size: 114 5593 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 2101 794 Size: 1307 4400 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 2056 2404 1184 Size: 872 348 4487 Fragments arranged by size: 4487 872 348 BstXI CCAn_nnnn'nTGG Cuts at: 2152 2152 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2187 2317 2628 2940 3037 3404 3789 3806 Size: 215 130 311 312 97 367 385 17 Cuts at: 3806 5258 5344 5356 5702 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 312 311 215 130 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2394 2446 513 Size: 552 1329 52 3774 Fragments arranged by size: 3774 1329 552 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2371 487 Size: 683 56 1145 3823 Fragments arranged by size: 3823 1145 683 56 BtsI GCAGTG_nn' Cuts at: 2367 3223 4017 4037 2367 Size: 856 794 20 4037 Fragments arranged by size: 4037 856 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 2662 3154 Size: 54 1425 50 275 18 561 271 492 Cuts at: 3154 4043 5672 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 561 492 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3637 1353 Size: 2284 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2677 2677 Size: 5707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2186 2246 2496 2619 2838 2993 Size: 393 463 91 60 250 123 219 155 Cuts at: 2993 3770 4367 5154 5422 1239 Size: 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 597 463 393 268 250 219 155 123 91 60 EcoO109I rG'GnC_Cy Cuts at: 2831 2831 Size: 5707 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 2082 2278 2472 4854 1678 Size: 404 196 194 2382 2531 Fragments arranged by size: 2531 2382 404 196 194 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373 Size: 32 135 32 2776 32 42 32 2626 Fragments arranged by size: 2776 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KasI G'GCGC_C Cuts at: 2274 2274 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4821 5005 1305 Size: 3516 184 2007 Fragments arranged by size: 3516 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 3665 4024 4183 518 Size: 648 1087 1412 359 159 2042 Fragments arranged by size: 2042 1412 1087 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2687 2696 2816 2936 3123 3800 4948 5193 Size: 1081 9 120 120 187 677 1148 245 Cuts at: 5193 1606 Size: 2120 Fragments arranged by size: 2120 1148 1081 677 245 187 120 120 9 NarI GG'CG_CC Cuts at: 2275 2275 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5155 NdeI CA'TA_TG Cuts at: 387 2769 387 Size: 2382 3325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NruI TCG'CGA Cuts at: 2085 2085 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 2115 2839 3059 830 Size: 1285 724 220 3478 Fragments arranged by size: 3478 1285 724 220 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2687 2936 2687 Size: 249 5458 SacI G_AGCT'C Cuts at: 2679 2679 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 2115 3059 2115 Size: 944 4763 ScaI AGT'ACT Cuts at: 2639 3955 5667 2639 Size: 1316 1712 2679 Fragments arranged by size: 2679 1712 1316 SfcI C'TryA_G Cuts at: 826 1045 2111 2835 3055 4190 4871 5062 826 Size: 219 1066 724 220 1135 681 191 1471 Fragments arranged by size: 1471 1135 1066 724 681 220 219 191 SfoI GGC'GCC Cuts at: 2276 2276 Size: 5707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 2159 2228 3034 1872 Size: 287 69 806 4545 Fragments arranged by size: 4545 806 287 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 3811 4712 4974 820 Size: 197 926 267 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 267 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2270 2862 2924 3146 513 Size: 552 1205 592 62 222 3074 Fragments arranged by size: 3074 1205 592 552 222 62 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2554 3734 3751 3904 4089 4534 325 Size: 1255 974 1180 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1180 974 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 3953 5665 Size: 274 80 33 51 1443 660 1316 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1443 1316 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 2157 2226 3032 1870 Size: 287 69 806 4545 Fragments arranged by size: 4545 806 287 69 XmnI GAAnn'nnTTC Cuts at: 2957 3836 2957 Size: 879 4828 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI