(Circular) (Six-base) MAPSORT of: pFN21AB7650.seq Check: 6958 from: 1 to: 5881 pFN21AB7650 5881 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3101 3101 Size: 5881 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2740 3224 1937 Size: 803 484 4594 Fragments arranged by size: 4594 803 484 Acc65I G'GTAC_C Cuts at: 3202 3202 Size: 5881 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2815 3195 3944 5328 5596 1632 Size: 1183 380 749 1384 268 1917 Fragments arranged by size: 1917 1384 1183 749 380 268 AfeI AGC'GCT Cuts at: 2063 2063 Size: 5881 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5881 AlwNI CAG_nnn'CTG Cuts at: 2971 3168 3624 5196 2971 Size: 197 456 1572 3656 Fragments arranged by size: 3656 1572 456 197 ApaLI G'TGCA_C Cuts at: 2220 3938 5094 2220 Size: 1718 1156 3007 Fragments arranged by size: 3007 1718 1156 ApoI r'AATT_y Cuts at: 1493 2423 3190 3416 3568 5677 1493 Size: 930 767 226 152 2109 1697 Fragments arranged by size: 2109 1697 930 767 226 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 2075 3206 1870 Size: 73 132 1131 4545 Fragments arranged by size: 4545 1131 132 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3131 3202 4657 618 Size: 325 217 627 1344 71 1455 1842 Fragments arranged by size: 1842 1455 1344 627 325 217 71 BanII G_rGCy'C Cuts at: 1391 3200 1391 Size: 1809 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4910 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2161 2195 4072 4106 1903 Size: 34 224 34 1877 34 3678 Fragments arranged by size: 3678 1877 224 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2319 3773 4983 2319 Size: 1454 1210 3217 Fragments arranged by size: 3217 1454 1210 BclI T'GATC_A Cuts at: 1548 2649 2730 3021 1548 Size: 1101 81 291 4408 Fragments arranged by size: 4408 1101 291 81 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2780 4492 136 Size: 107 122 71 631 605 1108 1712 1525 Fragments arranged by size: 1712 1525 1108 631 605 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 2224 3942 5098 1790 Size: 434 1718 1156 2573 Fragments arranged by size: 2573 1718 1156 434 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3178 3612 4570 Size: 416 53 83 399 24 1761 434 958 Cuts at: 4570 442 Size: 1753 Fragments arranged by size: 1761 1753 958 434 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3085 3085 Size: 5881 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2348 2786 3106 4541 1239 Size: 881 228 438 320 1435 2579 Fragments arranged by size: 2579 1435 881 438 320 228 Bpu10I CC'TnA_GC Cuts at: 2030 2030 Size: 5881 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4006 4871 5169 4006 Size: 865 298 4718 Fragments arranged by size: 4718 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4544 882 Size: 617 3045 2219 Fragments arranged by size: 3045 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3672 493 Size: 711 2468 2702 Fragments arranged by size: 2702 2468 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4314 4712 4986 5133 5544 4314 Size: 398 274 147 411 4651 Fragments arranged by size: 4651 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2351 3104 3134 1178 Size: 30 165 30 918 30 753 30 3925 Fragments arranged by size: 3925 918 753 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2930 1186 Size: 1744 4137 BseYI C'CCAG_C Cuts at: 2088 5084 5580 2088 Size: 2996 496 2389 Fragments arranged by size: 2996 2389 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3079 1163 Size: 573 1343 3965 Fragments arranged by size: 3965 1343 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 2137 3331 4092 5120 1540 Size: 275 186 111 25 1194 761 1028 2301 Fragments arranged by size: 2301 1194 1028 761 275 186 111 25 BsiHKAI G_wGCw'C Cuts at: 2224 3942 4027 5098 2224 Size: 1718 85 1071 3007 Fragments arranged by size: 3007 1718 1071 85 BsmI GAATG_Cn' Cuts at: 3392 3485 3392 Size: 93 5788 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2224 3200 3942 4027 5098 1391 Size: 399 434 976 742 85 1071 2174 Fragments arranged by size: 2174 1071 976 742 434 399 85 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3101 3236 844 Size: 2257 135 3489 Fragments arranged by size: 3489 2257 135 BsrBI CCG'CTC Cuts at: 1394 1823 3768 1394 Size: 429 1945 3507 Fragments arranged by size: 3507 1945 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3133 4376 4550 65 Size: 1183 1885 1243 174 1396 Fragments arranged by size: 1885 1396 1243 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2107 2136 4525 4712 1700 Size: 110 297 29 2389 187 2869 Fragments arranged by size: 2869 2389 297 187 110 29 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 3941 4953 3941 Size: 1012 4869 BstAPI GCAn_nnn'nTGC Cuts at: 794 3005 794 Size: 2211 3670 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 2123 2671 1184 Size: 939 548 4394 Fragments arranged by size: 4394 939 548 BstYI r'GATC_y Cuts at: 1972 3211 3578 3963 3980 5432 5518 5530 5876 Size: 1239 367 385 17 1452 86 12 346 Cuts at: 5876 1972 Size: 1977 Fragments arranged by size: 1977 1452 1239 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2131 513 Size: 552 939 127 4263 Fragments arranged by size: 4263 939 552 127 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 3058 3397 4191 4211 3058 Size: 339 794 20 4728 Fragments arranged by size: 4728 794 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4888 809 Size: 507 3572 1802 Fragments arranged by size: 3572 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2134 2781 Size: 54 1425 50 275 18 279 25 647 Cuts at: 2781 3328 4217 5846 8 Size: 547 889 1629 43 Fragments arranged by size: 1629 1425 889 647 547 279 275 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 2134 3328 1537 Size: 275 297 25 1194 4090 Fragments arranged by size: 4090 1194 297 275 25 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 3171 3811 1353 Size: 845 973 640 3423 Fragments arranged by size: 3423 973 845 640 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4468 4842 4988 387 Size: 1064 138 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 2348 2786 2815 3106 3195 3944 Size: 393 488 228 438 29 291 89 749 Cuts at: 3944 4541 5328 5596 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 749 597 488 438 393 291 268 228 89 29 EcoNI CCTnn'n_nnAGG Cuts at: 2282 3022 2282 Size: 740 5141 EcoO109I rG'GnC_Cy Cuts at: 2024 2332 2024 Size: 308 5573 EcoRI G'AATT_C Cuts at: 3190 3568 3190 Size: 378 5503 FseI GG_CCGG'CC Cuts at: 2111 2111 Size: 5881 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 2065 5028 1678 Size: 387 2963 2531 Fragments arranged by size: 2963 2531 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 2722 2754 2847 Size: 32 135 32 749 32 369 32 93 Cuts at: 2847 2879 3104 3136 4522 4554 4596 4628 1373 Size: 32 225 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1386 749 369 225 135 93 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 2032 748 Size: 1284 4597 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 3206 3206 Size: 5881 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2542 4995 5179 1305 Size: 1237 2453 184 2007 Fragments arranged by size: 2453 2007 1237 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2783 10 Size: 54 1425 343 951 3108 Fragments arranged by size: 3108 1425 951 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2657 3839 4198 4357 518 Size: 648 1491 1182 359 159 2042 Fragments arranged by size: 2042 1491 1182 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2133 2161 2347 2803 3297 3974 5122 5367 Size: 527 28 186 456 494 677 1148 245 Cuts at: 5367 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 527 494 456 245 186 28 NaeI GCC'GGC Cuts at: 2109 2138 2109 Size: 29 5852 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4390 Fragments arranged by size: 4390 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NgoMIV G'CCGG_C Cuts at: 2107 2136 2107 Size: 29 5852 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 2440 2978 3239 4784 1132 Size: 97 1211 538 261 1545 2229 Fragments arranged by size: 2229 1545 1211 538 261 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2024 Size: 5881 PshAI GACnn'nnGTC Cuts at: 1613 2247 1613 Size: 634 5247 PsiI TTA'TAA Cuts at: 3451 3451 Size: 5881 PstI C_TGCA'G Cuts at: 830 3233 830 Size: 2403 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2347 2347 Size: 5881 SacII CC_GC'GG Cuts at: 2134 2134 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SanDI GG'GwC_CC Cuts at: 2024 2024 Size: 5881 SapI GCTCTTCn'nnn_ Cuts at: 2198 2198 Size: 5881 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2963 3229 4364 5045 5236 826 Size: 219 1918 266 1135 681 191 1471 Fragments arranged by size: 1918 1471 1135 681 266 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3985 4886 5148 820 Size: 197 926 2042 901 262 1553 Fragments arranged by size: 2042 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3805 5 Size: 47 3753 2081 Fragments arranged by size: 3753 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2004 2501 3320 513 Size: 552 939 497 819 3074 Fragments arranged by size: 3074 939 819 552 497 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3908 3925 4078 4263 4708 325 Size: 1255 2328 17 153 185 445 1498 Fragments arranged by size: 2328 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4127 5839 96 Size: 274 80 33 51 1443 2150 1712 138 Fragments arranged by size: 2150 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3088 3120 5841 5873 3088 Size: 32 2721 32 3096 Fragments arranged by size: 3096 2721 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5881 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 4010 4010 Size: 5881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI XcmI