Restriction map for
pFN21AB7641
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
AlwI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BbsI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DrdI Eam1105I EarI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MboI MboII MfeI MflI MluNI
MroI MscI MseI MslI MunI
NcoI NdeI NdeII NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SalI SapI Sau3AI SbfI
ScaI SfcI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflIII 746 ACGCGT A^C[GA][CT]GT
Alw44I 157 GTGCAC G^TGCAC
AlwNI 199 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 157 GTGCAC G^TGCAC
BbrPI 871 CACGTG CAC^GTG
BbvCI 421 GCTGAGG CCTCAGC(-5/-2)
BcgI 848 GCAAGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 514 CTAG C^TAG
BpmI 283 CTGGAG CTGGAG(16/14)
BsaAI 871 CACGTG [CT]AC^GT[GA]
BsgI 240 GTGCAG GTGCAG(16/14)
BsiEI 897 CGGCCG CG[GA][CT]^CG
BsrBI 258 GAGCGG CCGCTC(-3/-3)
DraIII 787 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 897 CGGCCG C^GGCCG
EclXI 897 CGGCCG C^GGCCG
Eco52I 897 CGGCCG C^GGCCG
EcoNI 279 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI 573 TCACC GGTGA(8/7)
KspI 100 CCGCGG CCGC^GG
MaeI 514 CTAG C^TAG
MaeIII 349 GTGAC ^GT[ACGT]AC
MluI 746 ACGCGT A^CGCGT
PmaCI 871 CACGTG CAC^GTG
PmlI 871 CACGTG CAC^GTG
SacI 289 GAGCTC GAGCT^C
SacII 100 CCGCGG CCGC^GG
SexAI 788 ACCTGGT A^CC[AT]GGT
SfaNI 890 GCATC GCATC(5/9)
TfiI 396 GATTC G^A[AT]TC
Tsp45I 349 GTGAC ^GT[GC]AC
TspRI 387 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 340 CCTCAGG CC^T[ACGT]AGG
401 CCTTAGG
BglI 412 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
596 GCCACTCGGGC
BseRI 265 GAGGAG GAGGAG(10/8)
286 GAGGAG
BspMI 575 ACCTGC ACCTGC(4/8)
659 ACCTGC
BsrI 140 ACTGG ACTGG(1/-1)
776 ACTGG
BssHII 209 GCGCGC G^CGCGC
914 GCGCGC
BstAPI 786 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
870 GCACGTGCTGC
Bsu36I 340 CCTCAGG CC^T[ACGT]AGG
401 CCTTAGG
EaeI 897 CGGCCG [CT]^GGCC[GA]
908 CGGCCA
Eco81I 340 CCTCAGG CC^T[ACGT]AGG
401 CCTTAGG
EcoT14I 164 CCAAGG C^C[AT][AT]GG
971 CCTTGG
NaeI 245 GCCGGC GCC^GGC
429 GCCGGC
NgoMIV 245 GCCGGC G^CCGGC
429 GCCGGC
PleI 49 GACTC GAGTC(4/5)
442 GACTC
SmaI 522 CCCGGG CCC^GGG
703 CCCGGG
StuI 230 AGGCCT AGG^CCT
638 AGGCCT
StyI 164 CCAAGG C^C[AT][AT]GG
971 CCTTGG
XmaCI 522 CCCGGG C^CCGGG
703 CCCGGG
XmaI 522 CCCGGG C^CCGGG
703 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaI BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIV Sau96I
ScrFI SfoI TseI