(Circular) (Six-base) MAPSORT of: pFN21AB7641.seq Check: 2350 from: 1 to: 5683 pFN21AB7641 5683 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2589 2673 2589 Size: 84 5599 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5130 5398 1632 Size: 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2751 4582 5559 1203 Size: 1548 1831 977 1327 Fragments arranged by size: 1831 1548 1327 977 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5683 AlwNI CAG_nnn'CTG Cuts at: 2209 3426 4998 2209 Size: 1217 1572 2894 Fragments arranged by size: 2894 1572 1217 ApaI G_GGCC'C Cuts at: 2105 2366 2715 2945 2105 Size: 261 349 230 4843 Fragments arranged by size: 4843 349 261 230 ApaLI G'TGCA_C Cuts at: 2162 3740 4896 2162 Size: 1578 1156 2949 Fragments arranged by size: 2949 1578 1156 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2156 2527 2546 2605 2708 2818 3008 Size: 73 213 371 19 59 103 110 190 Cuts at: 3008 1870 Size: 4545 Fragments arranged by size: 4545 371 213 190 110 103 73 59 19 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2017 2064 2341 2411 2437 Size: 325 217 627 230 47 277 70 26 Cuts at: 2437 2497 2531 2670 2865 3004 4459 618 Size: 60 34 139 195 139 1455 1842 Fragments arranged by size: 1842 1455 627 325 277 230 217 195 139 139 70 60 47 34 26 BanII G_rGCy'C Cuts at: 1391 2105 2298 2366 2481 2715 2945 3002 1391 Size: 714 193 68 115 234 230 57 4072 Fragments arranged by size: 4072 714 234 230 193 115 68 57 BbeI G_GCGC'C Cuts at: 2441 2501 2674 2869 2441 Size: 60 173 195 5255 Fragments arranged by size: 5255 195 173 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4712 BbvCI CC'TCA_GC Cuts at: 2427 2427 Size: 5683 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2842 2876 3874 3908 1903 Size: 34 905 34 998 34 3678 Fragments arranged by size: 3678 998 905 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2423 2607 4294 Size: 107 122 71 631 605 751 184 1687 Cuts at: 4294 136 Size: 1525 Fragments arranged by size: 1687 1525 751 631 605 184 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 2022 2105 2166 2366 2534 2715 2945 3744 Size: 232 83 61 200 168 181 230 799 Cuts at: 3744 4900 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 799 232 230 200 181 168 83 61 BmgBI CAC'GTC Cuts at: 1318 2538 1318 Size: 1220 4463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3414 4372 442 Size: 416 53 83 399 24 1997 958 1753 Fragments arranged by size: 1997 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2361 2361 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 4343 1239 Size: 1070 2034 2579 Fragments arranged by size: 2579 2034 1070 Bpu10I CC'TnA_GC Cuts at: 2210 2427 2210 Size: 217 5466 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4346 882 Size: 617 2847 2219 Fragments arranged by size: 2847 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2878 3474 493 Size: 711 1674 596 2702 Fragments arranged by size: 2702 1674 711 596 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2438 2498 2671 2866 Size: 53 83 186 936 905 60 173 195 Cuts at: 2866 3490 3872 4507 275 Size: 624 382 635 1451 Fragments arranged by size: 1451 936 905 635 624 382 195 186 173 83 60 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 1178 Size: 30 165 30 762 30 4666 Fragments arranged by size: 4666 762 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 2306 1186 Size: 1099 21 4563 Fragments arranged by size: 4563 1099 21 BseYI C'CCAG_C Cuts at: 2116 2480 2841 4886 5382 2116 Size: 364 361 2045 496 2417 Fragments arranged by size: 2417 2045 496 364 361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2266 1163 Size: 573 530 4580 Fragments arranged by size: 4580 573 530 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2905 3133 3894 4922 1540 Size: 275 186 904 228 761 1028 2301 Fragments arranged by size: 2301 1028 904 761 275 228 186 BsiHKAI G_wGCw'C Cuts at: 2166 2298 2664 2793 2877 3744 3829 4900 2166 Size: 132 366 129 84 867 85 1071 2949 Fragments arranged by size: 2949 1071 867 366 132 129 85 84 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2105 2166 2298 2366 2481 2534 Size: 399 232 83 61 132 68 115 53 Cuts at: 2534 2664 2715 2793 2877 2945 3002 3744 3829 Size: 130 51 78 84 68 57 742 85 Cuts at: 3829 4900 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 232 132 130 115 85 84 83 78 68 68 61 57 53 51 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2589 2673 3038 844 Size: 1745 84 365 3489 Fragments arranged by size: 3489 1745 365 84 BsrBI CCG'CTC Cuts at: 1394 1823 2265 3570 1394 Size: 429 442 1305 3507 Fragments arranged by size: 3507 1305 442 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2250 2434 2592 4327 4514 1700 Size: 110 440 184 158 1735 187 2869 Fragments arranged by size: 2869 1735 440 187 184 158 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 2214 2919 1925 Size: 289 705 4689 Fragments arranged by size: 4689 705 289 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 2797 2881 794 Size: 2003 84 3596 Fragments arranged by size: 3596 2003 84 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5683 BstYI r'GATC_y Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678 Size: 1041 367 385 17 1452 86 12 346 Cuts at: 5678 1972 Size: 1977 Fragments arranged by size: 1977 1452 1041 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2346 2407 2346 Size: 61 5622 BtgI C'CryG_G Cuts at: 513 1065 2004 2060 2105 2899 513 Size: 552 939 56 45 794 3297 Fragments arranged by size: 3297 939 794 552 56 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 3199 3993 4013 3199 Size: 794 20 4869 Fragments arranged by size: 4869 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DraIII CAC_nnn'GTG Cuts at: 2797 2797 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2902 2913 3130 Size: 54 1425 50 275 18 1072 11 217 Cuts at: 3130 4019 5648 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1072 889 275 217 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2902 3130 1537 Size: 275 1090 228 4090 Fragments arranged by size: 4090 1090 275 228 EarI CTCTTCn'nnn_ Cuts at: 1353 3613 1353 Size: 2260 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 4270 4644 4790 387 Size: 1064 138 474 2207 374 146 1280 Fragments arranged by size: 2207 1280 1064 474 374 146 138 EcoICRI GAG'CTC Cuts at: 2296 2296 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2309 3746 4343 5130 5398 1239 Size: 393 677 1437 597 787 268 1524 Fragments arranged by size: 1524 1437 787 677 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2288 2288 Size: 5683 EcoO109I rG'GnC_Cy Cuts at: 2041 2362 2363 2957 2041 Size: 321 1 594 4767 Fragments arranged by size: 4767 594 321 1 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 2051 2117 2441 2501 2674 2869 4830 1678 Size: 373 66 324 60 173 195 1961 2531 Fragments arranged by size: 2531 1961 373 324 195 173 66 60 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 4324 4356 4398 Size: 32 135 32 740 32 1980 32 42 Cuts at: 4398 4430 1373 Size: 32 2626 Fragments arranged by size: 2626 1980 740 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KasI G'GCGC_C Cuts at: 2437 2497 2670 2865 2437 Size: 60 173 195 5255 Fragments arranged by size: 5255 195 173 60 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 2751 5559 2751 Size: 2808 2875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2163 4797 4981 1305 Size: 858 2634 184 2007 Fragments arranged by size: 2634 2007 858 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3641 4000 4159 518 Size: 648 843 1632 359 159 2042 Fragments arranged by size: 2042 1632 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2494 2912 3099 3776 4924 5169 1606 Size: 501 387 418 187 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 501 418 387 245 187 NaeI GCC'GGC Cuts at: 2252 2436 2252 Size: 184 5499 NarI GG'CG_CC Cuts at: 2438 2498 2671 2866 2438 Size: 60 173 195 5255 Fragments arranged by size: 5255 195 173 60 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4192 Fragments arranged by size: 4192 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NgoMIV G'CCGG_C Cuts at: 2250 2434 2250 Size: 184 5499 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 3041 4586 1132 Size: 97 1812 1545 2229 Fragments arranged by size: 2229 1812 1545 97 PasI CC'CwG_GG Cuts at: 1348 2366 2420 1348 Size: 1018 54 4611 Fragments arranged by size: 4611 1018 54 PciI A'CATG_T Cuts at: 4582 4582 Size: 5683 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PmlI CAC'GTG Cuts at: 2878 2878 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5683 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PspOMI G'GGCC_C Cuts at: 2101 2362 2711 2941 2101 Size: 261 349 230 4843 Fragments arranged by size: 4843 349 261 230 PstI C_TGCA'G Cuts at: 830 3035 830 Size: 2205 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 SacI G_AGCT'C Cuts at: 2298 2298 Size: 5683 SacII CC_GC'GG Cuts at: 2108 2108 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SexAI A'CCwGG_T Cuts at: 2793 2793 Size: 5683 SfcI C'TryA_G Cuts at: 826 1045 3031 4166 4847 5038 826 Size: 219 1986 1135 681 191 1471 Fragments arranged by size: 1986 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2439 2499 2672 2867 2439 Size: 60 173 195 5255 Fragments arranged by size: 5255 195 173 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 2529 2710 3010 1872 Size: 657 181 300 4545 Fragments arranged by size: 4545 657 300 181 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2237 2645 1517 Size: 720 408 4555 Fragments arranged by size: 4555 720 408 StyI C'CwwG_G Cuts at: 513 1065 2004 2169 2976 3122 513 Size: 552 939 165 807 146 3074 Fragments arranged by size: 3074 939 807 552 165 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2230 2969 3710 3727 3880 4065 4510 Size: 1255 650 739 741 17 153 185 445 Cuts at: 4510 325 Size: 1498 Fragments arranged by size: 1498 1255 741 739 650 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3929 5641 96 Size: 274 80 33 51 1443 1952 1712 138 Fragments arranged by size: 1952 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2045 2077 5643 5675 2045 Size: 32 3566 32 2053 Fragments arranged by size: 3566 2053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 2527 2708 3008 1870 Size: 657 181 300 4545 Fragments arranged by size: 4545 657 300 181 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI XcmI