Restriction map for
pFN21AF0386
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BciVI BfaI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BstBI BstYI
Bsu36I ClaI CpoI DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT14I
FseI FspI HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TfiI
Tth111I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 795 GTATAC GT^[AC][GT]AC
AccII 1142 CGCG CG^CG
AcsI 814 AAATTT [GA]^AATT[CT]
AcyI 1888 GACGCC G[GA]^CG[CT]C
ApaI 1985 GGGCCC GGGCC^C
ApoI 814 AAATTT [GA]^AATT[CT]
AvaI 152 CCCGGG C^[CT]CG[GA]G
BbvCI 1237 GCTGAGG CCTCAGC(-5/-2)
BcgI 830 CGATGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1304 TGATCA T^GATCA
BlpI 352 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 352 GCTCAGC GC^T[ACGT]AGC
BsaHI 1888 GACGCC G[GA]^CG[CT]C
BsaI 19 GAGACC GGTCTC(1/5)
BseRI 1240 GAGGAG GAGGAG(10/8)
BsoBI 152 CCCGGG C^[CT]CG[GA]G
BspHI 1211 TCATGA T^CATGA
BsrFI 1871 ACCGGC [GA]^CCGG[CT]
BssSI 172 CTCGTG CACGAG(-5/-1)
Bst1107I 795 GTATAC GTA^TAC
BstEII 1962 GGTCACC G^GT[ACGT]ACC
BstPI 1962 GGTCACC G^GT[ACGT]ACC
BstUI 1142 CGCG CG^CG
BstXI 1718 CCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 795 GTATAC GTA^TAC
CelII 352 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1871 ACCGGC [GA]^CCGG[CT]
DraI 1182 TTTAAA TTT^AAA
DrdI 987 GACCCCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1356 GAAGAG CTCTTC(1/4)
EcoO65I 1962 GGTCACC G^GT[ACGT]ACC
EcoT22I 655 ATGCAT ATGCA^T
FbaI 1304 TGATCA T^GATCA
Hin1I 1888 GACGCC G[GA]^CG[CT]C
HindIII 224 AAGCTT A^AGCTT
HinfI 923 GAGTC G^A[ACGT]TC
Ksp632I 1356 GAAGAG CTCTTC(1/4)
MvnI 1142 CGCG CG^CG
NsiI 655 ATGCAT ATGCA^T
PleI 923 GAGTC GAGTC(4/5)
RcaI 1211 TCATGA T^CATGA
SapI 1356 GAAGAGC GCTCTTC(1/4)
SmaI 152 CCCGGG CCC^GGG
StuI 2003 AGGCCT AGG^CCT
XcmI 110 CCAGCAAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 152 CCCGGG C^CCGGG
XmaI 152 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 1635 GTGCAC G^TGCAC
1912 GTGCAC
ApaLI 1635 GTGCAC G^TGCAC
1912 GTGCAC
BanI 1458 GGTGCC G^G[CT][GA]CC
1482 GGTGCC
BglI 1217 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1528 GCCGTCCTGGC
BsmAI 19 GAGAC GTCTC(1/5)
200 GAGAC
BspMI 255 GCAGGT ACCTGC(4/8)
978 GCAGGT
DraII 1340 AGGACCT [GA]G^G[ACGT]CC[CT]
1796 AGGACCT
EcoO109I 1340 AGGACCT [GA]G^G[ACGT]CC[CT]
1796 AGGACCT
HaeII 261 GGCGCT [GA]GCGC^[CT]
312 GGCGCT
HgaI 1888 GACGC GACGC(5/10)
1994 GCGTC
MaeII 5 ACGT A^CGT
1061 ACGT
MseI 440 TTAA T^TAA
1183 TTAA
MslI 1188 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1452 CATGGCGGTG
PpuMI 1340 AGGACCT [GA]G^G[AT]CC[CT]
1796 AGGACCT
SacI 824 GAGCTC GAGCT^C
1822 GAGCTC
Tru9I 440 TTAA T^TAA
1183 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BalI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrBI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I