(Circular) (Six-base) MAPSORT of: pFN21AF0386.seq Check: 6408 from: 1 to: 6727 pFN21AF0386 6727 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2251 2974 2251 Size: 723 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4537 278 Size: 53 83 186 936 3001 2468 Fragments arranged by size: 3001 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2801 4070 1937 Size: 864 1269 4594 Fragments arranged by size: 4594 1269 864 Acc65I G'GTAC_C Cuts at: 4048 4048 Size: 6727 AclI AA'CG_TT Cuts at: 1563 4854 5227 1563 Size: 3291 373 3063 Fragments arranged by size: 3291 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2165 3148 3290 3428 4790 6174 6442 1632 Size: 533 983 142 138 1362 1384 268 1917 Fragments arranged by size: 1917 1384 1362 983 533 268 142 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6530 AflIII A'CryG_T Cuts at: 1203 5626 6603 1203 Size: 4423 977 1327 Fragments arranged by size: 4423 1327 977 AgeI A'CCGG_T Cuts at: 5558 5558 Size: 6727 AhdI GACnn_n'nnGTC Cuts at: 5456 5456 Size: 6727 AleI CACnn'nnGTG Cuts at: 1166 3197 1166 Size: 2031 4696 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6727 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3710 3742 3710 Size: 32 6695 AlwNI CAG_nnn'CTG Cuts at: 2059 2494 2638 2650 3259 4470 6042 2059 Size: 435 144 12 609 1211 1572 2744 Fragments arranged by size: 2744 1572 1211 609 435 144 12 ApaI G_GGCC'C Cuts at: 3994 3994 Size: 6727 ApaLI G'TGCA_C Cuts at: 3640 3917 4784 5940 3640 Size: 277 867 1156 4427 Fragments arranged by size: 4427 1156 867 277 ApoI r'AATT_y Cuts at: 1493 2819 4036 4262 4414 6523 1493 Size: 1326 1217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1326 1217 226 152 AseI AT'TA_AT Cuts at: 160 1032 5281 160 Size: 872 4249 1606 Fragments arranged by size: 4249 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 AvaI C'yCGr_G Cuts at: 1870 1943 2157 4052 1870 Size: 73 214 1895 4545 Fragments arranged by size: 4545 1895 214 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3491 3524 3491 Size: 33 6694 BamHI G'GATC_C Cuts at: 4057 4424 4057 Size: 367 6360 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3463 3487 4048 5503 618 Size: 325 217 627 1676 24 561 1455 1842 Fragments arranged by size: 1842 1676 1455 627 561 325 217 24 BanII G_rGCy'C Cuts at: 1391 2833 3137 3224 3297 3831 3994 4046 1391 Size: 1442 304 87 73 534 163 52 4072 Fragments arranged by size: 4072 1442 534 304 163 87 73 52 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3339 3416 3553 928 Size: 971 1440 77 137 4102 Fragments arranged by size: 4102 1440 971 137 77 BbvCI CC'TCA_GC Cuts at: 3243 3243 Size: 6727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2824 2858 4918 4952 1903 Size: 34 887 34 2060 34 3678 Fragments arranged by size: 3678 2060 887 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4619 5829 4619 Size: 1210 5517 BclI T'GATC_A Cuts at: 1548 3309 1548 Size: 1761 4966 BfrBI ATG'CAT Cuts at: 2662 2662 Size: 6727 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3228 3539 5338 Size: 107 122 71 631 605 1556 311 1799 Cuts at: 5338 136 Size: 1525 Fragments arranged by size: 1799 1556 1525 631 605 311 122 107 71 BglII A'GATC_T Cuts at: 6722 6722 Size: 6727 BlpI GC'TnA_GC Cuts at: 2358 4144 2358 Size: 1786 4941 Bme1580I G_kGCm'C Cuts at: 1790 2245 3468 3644 3840 3921 3994 4788 5944 Size: 455 1223 176 196 81 73 794 1156 Cuts at: 5944 1790 Size: 2573 Fragments arranged by size: 2573 1223 1156 794 455 196 176 81 73 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6727 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2849 3087 3131 Size: 416 53 83 399 24 1432 238 44 Cuts at: 3131 3626 3681 4458 5416 442 Size: 495 55 777 958 1753 Fragments arranged by size: 1753 1432 958 777 495 416 399 238 83 55 53 44 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6727 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2240 2399 2691 3217 3458 3680 3845 3893 Size: 1001 159 292 526 241 222 165 48 Cuts at: 3893 3919 5387 1239 Size: 26 1468 2579 Fragments arranged by size: 2579 1468 1001 526 292 241 222 165 159 48 26 Bpu10I CC'TnA_GC Cuts at: 2901 3243 2901 Size: 342 6385 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2211 3431 3680 4852 5717 6015 2211 Size: 1220 249 1172 865 298 2923 Fragments arranged by size: 2923 1220 1172 865 298 249 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 5390 882 Size: 617 519 3372 2219 Fragments arranged by size: 3372 2219 617 519 BsaAI yAC'GTr Cuts at: 493 1204 4518 493 Size: 711 3314 2702 Fragments arranged by size: 3314 2702 711 BsaBI GATnn'nnATC Cuts at: 6721 6721 Size: 6727 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3894 4534 4916 5551 Size: 53 83 186 936 2361 640 382 635 Cuts at: 5551 275 Size: 1451 Fragments arranged by size: 2361 1451 936 640 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5160 5558 5832 5979 6390 5160 Size: 398 274 147 411 5497 Fragments arranged by size: 5497 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2919 2949 3239 3269 1178 Size: 30 165 30 1516 30 290 30 4636 Fragments arranged by size: 4636 1516 290 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3260 1186 Size: 2074 4653 BseYI C'CCAG_C Cuts at: 2244 2580 2643 2697 2754 3018 3044 3107 3206 Size: 336 63 54 57 264 26 63 99 Cuts at: 3206 3223 5930 6426 2244 Size: 17 2707 496 2545 Fragments arranged by size: 2707 2545 496 336 264 99 63 63 57 54 26 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2417 2864 2879 2891 3008 1163 Size: 573 681 447 15 12 117 4882 Fragments arranged by size: 4882 681 573 447 117 15 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4177 4938 5966 1540 Size: 275 186 2176 761 1028 2301 Fragments arranged by size: 2301 2176 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2042 2833 3644 3696 3831 3921 4788 4873 5944 Size: 791 811 52 135 90 867 85 1071 Cuts at: 5944 2042 Size: 2825 Fragments arranged by size: 2825 1071 867 811 791 135 90 85 52 BsmI GAATG_Cn' Cuts at: 4238 4331 4238 Size: 93 6634 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2245 2833 3137 3224 3297 3468 Size: 399 252 203 588 304 87 73 171 Cuts at: 3468 3644 3696 3831 3840 3921 3994 4046 4788 Size: 176 52 135 9 81 73 52 742 Cuts at: 4788 4873 5944 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 399 304 252 203 176 171 135 87 85 81 73 73 52 52 9 BspEI T'CCGG_A Cuts at: 6390 6390 Size: 6727 BspHI T'CATG_A Cuts at: 3216 4616 6346 3216 Size: 1400 1730 3597 Fragments arranged by size: 3597 1730 1400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2251 2974 4082 844 Size: 1407 723 1108 3489 Fragments arranged by size: 3489 1407 1108 723 BsrBI CCG'CTC Cuts at: 1394 1823 3523 3552 3673 4614 1394 Size: 429 1700 29 121 941 3507 Fragments arranged by size: 3507 1700 941 429 121 29 BsrDI GCAATG_nn' Cuts at: 65 1248 5222 5396 65 Size: 1183 3974 174 1396 Fragments arranged by size: 3974 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3876 5371 5558 1700 Size: 110 2066 1495 187 2869 Fragments arranged by size: 2869 2066 1495 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6727 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6727 BssSI C'ACGA_G Cuts at: 2177 4787 5799 2177 Size: 2610 1012 3105 Fragments arranged by size: 3105 2610 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2572 2659 3004 794 Size: 1778 87 345 4517 Fragments arranged by size: 4517 1778 345 87 BstBI TT'CG_AA Cuts at: 5531 5531 Size: 6727 BstEII G'GTnAC_C Cuts at: 1184 3967 1184 Size: 2783 3944 BstXI CCAn_nnnn'nTGG Cuts at: 3730 3730 Size: 6727 BstYI r'GATC_y Cuts at: 1972 4057 4424 4809 4826 6278 6364 6376 6722 Size: 2085 367 385 17 1452 86 12 346 Cuts at: 6722 1972 Size: 1977 Fragments arranged by size: 2085 1977 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2802 2802 Size: 6727 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5236 Fragments arranged by size: 5236 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5988 Fragments arranged by size: 5988 683 56 BtsI GCAGTG_nn' Cuts at: 3751 3887 4243 5037 5057 3751 Size: 136 356 794 20 5421 Fragments arranged by size: 5421 794 356 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6727 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6692 DraI TTT'AAA Cuts at: 3189 4031 3189 Size: 842 5885 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2998 5734 809 Size: 507 1682 2736 1802 Fragments arranged by size: 2736 1802 1682 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 3037 3540 Size: 54 1425 50 275 18 351 856 503 Cuts at: 3540 3708 4174 5063 6692 8 Size: 168 466 889 1629 43 Fragments arranged by size: 1629 1425 889 856 503 466 351 275 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4174 1537 Size: 275 2362 4090 Fragments arranged by size: 4090 2362 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3356 4657 1353 Size: 2003 1301 3423 Fragments arranged by size: 3423 2003 1301 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2050 2613 5314 5688 5834 387 Size: 1064 138 461 563 2701 374 146 1280 Fragments arranged by size: 2701 1280 1064 563 461 374 146 138 EcoICRI GAG'CTC Cuts at: 2831 3829 2831 Size: 998 5729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2240 2399 2691 3148 3217 3290 Size: 393 533 75 159 292 457 69 73 Cuts at: 3290 3428 3458 3680 3845 3893 3919 4790 5387 Size: 138 30 222 165 48 26 871 597 Cuts at: 5387 6174 6442 1239 Size: 787 268 1524 Fragments arranged by size: 1524 871 787 597 533 457 393 292 268 222 165 159 138 75 73 69 48 30 26 EcoO109I rG'GnC_Cy Cuts at: 3346 3802 3346 Size: 456 6271 EcoRI G'AATT_C Cuts at: 4036 4414 4036 Size: 378 6349 FspI TGC'GCA Cuts at: 5233 5233 Size: 6727 HaeII r_GCGC'y Cuts at: 1678 2270 2321 5874 1678 Size: 592 51 3553 2531 Fragments arranged by size: 3553 2531 592 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2905 2919 2937 2951 2995 Size: 32 135 32 1333 14 18 14 44 Cuts at: 2995 3027 3947 3979 5368 5400 5442 5474 1373 Size: 32 920 32 1389 32 42 32 2626 Fragments arranged by size: 2626 1389 1333 920 135 44 42 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 4071 4317 669 Size: 988 281 2133 246 3079 Fragments arranged by size: 3079 2133 988 281 246 HindIII A'AGCT_T Cuts at: 748 2229 748 Size: 1481 5246 HpaI GTT'AAC Cuts at: 4317 4317 Size: 6727 KpnI G_GTAC'C Cuts at: 4052 4052 Size: 6727 MfeI C'AATT_G Cuts at: 4326 4326 Size: 6727 MluI A'CGCG_T Cuts at: 6603 6603 Size: 6727 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2545 3087 5841 6025 1305 Size: 1240 542 2754 184 2007 Fragments arranged by size: 2754 2007 1240 542 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2183 3542 3710 10 Size: 54 1425 343 351 1359 168 3027 Fragments arranged by size: 3027 1425 1359 351 343 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 3197 3461 4685 5044 5203 518 Size: 648 2031 264 1224 359 159 2042 Fragments arranged by size: 2042 2031 1224 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 2248 2494 2512 2650 3227 3277 3842 3865 Size: 642 246 18 138 577 50 565 23 Cuts at: 3865 3924 4143 4820 5968 6213 1606 Size: 59 219 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 577 565 246 245 219 138 59 50 23 18 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5236 Fragments arranged by size: 5236 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6727 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6727 NotI GC'GGCC_GC Cuts at: 4174 4174 Size: 6727 NsiI A_TGCA'T Cuts at: 2664 2664 Size: 6727 NspI r_CATG'y Cuts at: 1132 1229 4085 5630 1132 Size: 97 2856 1545 2229 Fragments arranged by size: 2856 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6727 PciI A'CATG_T Cuts at: 5626 5626 Size: 6727 PflMI CCAn_nnn'nTGG Cuts at: 1108 2263 2761 2917 1108 Size: 1155 498 156 4918 Fragments arranged by size: 4918 1155 498 156 PfoI T'CCnGG_A Cuts at: 2923 3525 3951 2923 Size: 602 426 5699 Fragments arranged by size: 5699 602 426 PmeI GTTT'AAAC Cuts at: 4031 4031 Size: 6727 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3194 3226 4796 4828 6309 6341 3194 Size: 32 1570 32 1481 32 3580 Fragments arranged by size: 3580 1570 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3346 3802 3346 Size: 456 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6727 PsiI TTA'TAA Cuts at: 4297 4297 Size: 6727 PspOMI G'GGCC_C Cuts at: 3990 3990 Size: 6727 PstI C_TGCA'G Cuts at: 830 2253 2274 2499 2517 2655 3758 3948 4079 Size: 1423 21 225 18 138 1103 190 131 Cuts at: 4079 830 Size: 3478 Fragments arranged by size: 3478 1423 1103 225 190 138 131 21 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6727 PvuII CAG'CTG Cuts at: 2248 2494 2512 2650 3227 3277 2248 Size: 246 18 138 577 50 5698 Fragments arranged by size: 5698 577 246 138 50 18 SacI G_AGCT'C Cuts at: 2833 3831 2833 Size: 998 5729 SalI G'TCGA_C Cuts at: 1936 4069 1936 Size: 2133 4594 SapI GCTCTTCn'nnn_ Cuts at: 3356 3356 Size: 6727 SbfI CC_TGCA'GG Cuts at: 4079 4079 Size: 6727 ScaI AGT'ACT Cuts at: 4975 6687 4975 Size: 1712 5015 SfcI C'TryA_G Cuts at: 826 1045 2249 2270 2321 2495 2513 2651 3754 Size: 219 1204 21 51 174 18 138 1103 Cuts at: 3754 3944 4075 5210 5891 6082 826 Size: 190 131 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 1103 681 219 191 190 174 138 131 51 21 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6727 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6727 SmaI CCC'GGG Cuts at: 1872 2159 4054 1872 Size: 287 1895 4545 Fragments arranged by size: 4545 1895 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 2226 3446 3659 4831 5732 5994 Size: 197 926 283 1220 213 1172 901 262 Cuts at: 5994 820 Size: 1553 Fragments arranged by size: 1553 1220 1172 926 901 283 262 213 197 SnaBI TAC'GTA Cuts at: 493 4518 493 Size: 4025 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6727 SphI G_CATG'C Cuts at: 1132 4085 1132 Size: 2953 3774 SspI AAT'ATT Cuts at: 5 52 4651 5 Size: 47 4599 2081 Fragments arranged by size: 4599 2081 47 StuI AGG'CCT Cuts at: 1517 4010 1517 Size: 2493 4234 StyI C'CwwG_G Cuts at: 513 1065 2004 4166 513 Size: 552 939 2162 3074 Fragments arranged by size: 3074 2162 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2042 3634 3955 4754 4771 4924 5109 Size: 1255 462 1592 321 799 17 153 185 Cuts at: 5109 5554 325 Size: 445 1498 Fragments arranged by size: 1592 1498 1255 799 462 445 321 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2890 3285 4973 Size: 274 80 33 51 1443 913 395 1688 Cuts at: 4973 6685 96 Size: 1712 138 Fragments arranged by size: 1712 1688 1443 913 395 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2080 2096 2112 2128 3574 3606 6687 6719 2080 Size: 16 16 16 1446 32 3081 32 2088 Fragments arranged by size: 3081 2088 1446 32 32 16 16 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6727 XbaI T'CTAG_A Cuts at: 4063 4063 Size: 6727 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2122 2122 Size: 6727 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6727 XmaI C'CCGG_G Cuts at: 1870 2157 4052 1870 Size: 287 1895 4545 Fragments arranged by size: 4545 1895 287 XmnI GAAnn'nnTTC Cuts at: 4856 4856 Size: 6727 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4535 276 Size: 53 83 186 936 3001 2468 Fragments arranged by size: 3001 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI