Restriction map for
pFN21AF0386
(Linear) (Six-base) MAPSORT of: FLJ00386.seq Check: 4997 from: 1 to: 2022
FLJ00386.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 246 969 2022
Size: 246 723 1053
Fragments arranged by size:
1053 723 246
AccI GT'mk_AC
Cuts at: 0 796 2022
Size: 796 1226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 1143 1285 1423 2022
Size: 160 983 142 138 599
Fragments arranged by size:
983 599 160 142 138
AleI CACnn'nnGTG
Cuts at: 0 1192 2022
Size: 1192 830
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1705 1737 2022
Size: 1705 32 285
Fragments arranged by size:
1705 285 32
AlwNI CAG_nnn'CTG
Cuts at: 0 54 489 633 645 1254 2022
Size: 54 435 144 12 609 768
Fragments arranged by size:
768 609 435 144 54 12
ApaI G_GGCC'C
Cuts at: 0 1989 2022
Size: 1989 33
ApaLI G'TGCA_C
Cuts at: 0 1635 1912 2022
Size: 1635 277 110
Fragments arranged by size:
1635 277 110
ApoI r'AATT_y
Cuts at: 0 814 2022
Size: 814 1208
AvaI C'yCGr_G
Cuts at: 0 152 2022
Size: 152 1870
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1486 1519 2022
Size: 1486 33 503
Fragments arranged by size:
1486 503 33
BanI G'GyrC_C
Cuts at: 0 1458 1482 2022
Size: 1458 24 540
Fragments arranged by size:
1458 540 24
BanII G_rGCy'C
Cuts at: 0 828 1132 1219 1292 1826 1989 2022
Size: 828 304 87 73 534 163 33
Fragments arranged by size:
828 534 304 163 87 73 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1334 1411 1548 2022
Size: 1334 77 137 474
Fragments arranged by size:
1334 474 137 77
BbvCI CC'TCA_GC
Cuts at: 0 1238 2022
Size: 1238 784
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 819 853 2022
Size: 819 34 1169
Fragments arranged by size:
1169 819 34
BclI T'GATC_A
Cuts at: 0 1304 2022
Size: 1304 718
BfrBI ATG'CAT
Cuts at: 0 657 2022
Size: 657 1365
BglI GCCn_nnn'nGGC
Cuts at: 0 1223 1534 2022
Size: 1223 311 488
Fragments arranged by size:
1223 488 311
BlpI GC'TnA_GC
Cuts at: 0 353 2022
Size: 353 1669
Bme1580I G_kGCm'C
Cuts at: 0 240 1463 1639 1835 1916 1989 2022
Size: 240 1223 176 196 81 73 33
Fragments arranged by size:
1223 240 196 176 81 73 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 844 1082 1126 1621 1676 2022
Size: 844 238 44 495 55 346
Fragments arranged by size:
844 495 346 238 55 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 394 686 1212 1453 1675 1840 1888
Size: 235 159 292 526 241 222 165 48
Cuts at: 1888 1914 2022
Size: 26 108
Fragments arranged by size:
526 292 241 235 222 165 159 108
48 26
Bpu10I CC'TnA_GC
Cuts at: 0 896 1238 2022
Size: 896 342 784
Fragments arranged by size:
896 784 342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1426 1675 2022
Size: 206 1220 249 347
Fragments arranged by size:
1220 347 249 206
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 2022
Size: 13 2009
BsaHI Gr'CG_yC
Cuts at: 0 1889 2022
Size: 1889 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 914 944 1234 1264 2022
Size: 914 30 290 30 758
Fragments arranged by size:
914 758 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1255 2022
Size: 1255 767
BseYI C'CCAG_C
Cuts at: 0 239 575 638 692 749 1013 1039 1102
Size: 239 336 63 54 57 264 26 63
Cuts at: 1102 1201 1218 2022
Size: 99 17 804
Fragments arranged by size:
804 336 264 239 99 63 63 57
54 26 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 859 874 886 1003 2022
Size: 412 447 15 12 117 1019
Fragments arranged by size:
1019 447 412 117 15 12
BsiHKAI G_wGCw'C
Cuts at: 0 37 828 1639 1691 1826 1916 2022
Size: 37 791 811 52 135 90 106
Fragments arranged by size:
811 791 135 106 90 52 37
Bsp1286I G_dGCh'C
Cuts at: 0 37 240 828 1132 1219 1292 1463 1639
Size: 37 203 588 304 87 73 171 176
Cuts at: 1639 1691 1826 1835 1916 1989 2022
Size: 52 135 9 81 73 33
Fragments arranged by size:
588 304 203 176 171 135 87 81
73 73 52 37 33 9
BspHI T'CATG_A
Cuts at: 0 1211 2022
Size: 1211 811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 246 969 2022
Size: 246 723 1053
Fragments arranged by size:
1053 723 246
BsrBI CCG'CTC
Cuts at: 0 1518 1547 1668 2022
Size: 1518 29 121 354
Fragments arranged by size:
1518 354 121 29
BsrFI r'CCGG_y
Cuts at: 0 1871 2022
Size: 1871 151
BssSI C'ACGA_G
Cuts at: 0 172 2022
Size: 172 1850
BstAPI GCAn_nnn'nTGC
Cuts at: 0 567 654 999 2022
Size: 567 87 345 1023
Fragments arranged by size:
1023 567 345 87
BstEII G'GTnAC_C
Cuts at: 0 1962 2022
Size: 1962 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1725 2022
Size: 1725 297
BstZ17I GTA'TAC
Cuts at: 0 797 2022
Size: 797 1225
BtsI GCAGTG_nn'
Cuts at: 0 1746 1882 2022
Size: 1746 136 140
Fragments arranged by size:
1746 140 136
DraI TTT'AAA
Cuts at: 0 1184 2022
Size: 1184 838
DrdI GACnn_nn'nnGTC
Cuts at: 0 993 2022
Size: 993 1029
EaeI y'GGCC_r
Cuts at: 0 176 1032 1535 1703 2022
Size: 176 856 503 168 319
Fragments arranged by size:
856 503 319 176 168
EarI CTCTTCn'nnn_
Cuts at: 0 1351 2022
Size: 1351 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 45 608 2022
Size: 45 563 1414
Fragments arranged by size:
1414 563 45
EcoICRI GAG'CTC
Cuts at: 0 826 1824 2022
Size: 826 998 198
Fragments arranged by size:
998 826 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 235 394 686 1143 1212 1285 1423
Size: 160 75 159 292 457 69 73 138
Cuts at: 1423 1453 1675 1840 1888 1914 2022
Size: 30 222 165 48 26 108
Fragments arranged by size:
457 292 222 165 160 159 138 108
75 73 69 48 30 26
EcoO109I rG'GnC_Cy
Cuts at: 0 1341 1797 2022
Size: 1341 456 225
Fragments arranged by size:
1341 456 225
HaeII r_GCGC'y
Cuts at: 0 265 316 2022
Size: 265 51 1706
Fragments arranged by size:
1706 265 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 900 914 932 946 990 1022 1942 1974
Size: 900 14 18 14 44 32 920 32
Cuts at: 1974 2022
Size: 48
Fragments arranged by size:
920 900 48 44 32 32 18 14
14
HindIII A'AGCT_T
Cuts at: 0 224 2022
Size: 224 1798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1082 2022
Size: 540 542 940
Fragments arranged by size:
940 542 540
MscI TGG'CCA
Cuts at: 0 178 1537 1705 2022
Size: 178 1359 168 317
Fragments arranged by size:
1359 317 178 168
MslI CAynn'nnrTG
Cuts at: 0 1192 1456 2022
Size: 1192 264 566
Fragments arranged by size:
1192 566 264
MspA1I CmG'CkG
Cuts at: 0 243 489 507 645 1222 1272 1837 1860
Size: 243 246 18 138 577 50 565 23
Cuts at: 1860 1919 2022
Size: 59 103
Fragments arranged by size:
577 565 246 243 138 103 59 50
23 18
NsiI A_TGCA'T
Cuts at: 0 659 2022
Size: 659 1363
PflMI CCAn_nnn'nTGG
Cuts at: 0 258 756 912 2022
Size: 258 498 156 1110
Fragments arranged by size:
1110 498 258 156
PfoI T'CCnGG_A
Cuts at: 0 918 1520 1946 2022
Size: 918 602 426 76
Fragments arranged by size:
918 602 426 76
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 2022
Size: 1189 32 801
Fragments arranged by size:
1189 801 32
PpuMI rG'GwC_Cy
Cuts at: 0 1341 1797 2022
Size: 1341 456 225
Fragments arranged by size:
1341 456 225
PspOMI G'GGCC_C
Cuts at: 0 1985 2022
Size: 1985 37
PstI C_TGCA'G
Cuts at: 0 248 269 494 512 650 1753 1943 2022
Size: 248 21 225 18 138 1103 190 79
Fragments arranged by size:
1103 248 225 190 138 79 21 18
PvuII CAG'CTG
Cuts at: 0 243 489 507 645 1222 1272 2022
Size: 243 246 18 138 577 50 750
Fragments arranged by size:
750 577 246 243 138 50 18
SacI G_AGCT'C
Cuts at: 0 828 1826 2022
Size: 828 998 196
Fragments arranged by size:
998 828 196
SapI GCTCTTCn'nnn_
Cuts at: 0 1351 2022
Size: 1351 671
SfcI C'TryA_G
Cuts at: 0 244 265 316 490 508 646 1749 1939
Size: 244 21 51 174 18 138 1103 190
Cuts at: 1939 2022
Size: 83
Fragments arranged by size:
1103 244 190 174 138 83 51 21
18
SmaI CCC'GGG
Cuts at: 0 154 2022
Size: 154 1868
SmlI C'TyrA_G
Cuts at: 0 221 1441 1654 2022
Size: 221 1220 213 368
Fragments arranged by size:
1220 368 221 213
StuI AGG'CCT
Cuts at: 0 2005 2022
Size: 2005 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 1629 1950 2022
Size: 37 1592 321 72
Fragments arranged by size:
1592 321 72 37
TatI w'GTAC_w
Cuts at: 0 885 1280 2022
Size: 885 395 742
Fragments arranged by size:
885 742 395
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 75 91 107 123 1569 1601 2022
Size: 75 16 16 16 1446 32 421
Fragments arranged by size:
1446 421 75 32 16 16 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 117 2022
Size: 117 1905
XmaI C'CCGG_G
Cuts at: 0 152 2022
Size: 152 1870
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI
BclI BfrBI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI BstZ17I BtsI DraI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HindIII MmeI MscI
MslI MspA1I NsiI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SapI SfcI SmaI SmlI StuI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AscI AseI AsiSI AvrII BamHI BbeI BciVI
BglII BmgBI BmtI BplI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssHII BstBI
BstYI Bsu36I BtgI BtgZI ClaI CspCI DraIII EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StyI SwaI Tth111I XbaI XhoI XmnI ZraI