(Circular) (Six-base) MAPSORT of: pFN21AF0343.seq Check: 5552 from: 1 to: 12622 pFN21AF0343 12622 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5737 5737 Size: 12622 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4416 4731 9192 10432 Size: 53 83 186 936 2880 315 4461 1240 Cuts at: 10432 278 Size: 2468 Fragments arranged by size: 4461 2880 2468 1240 936 315 186 83 53 AccI GT'mk_AC Cuts at: 1937 2067 4326 6504 6815 9585 9720 9965 1937 Size: 130 2259 2178 311 2770 135 245 4594 Fragments arranged by size: 4594 2770 2259 2178 311 245 135 130 Acc65I G'GTAC_C Cuts at: 6592 9943 6592 Size: 3351 9271 AclI AA'CG_TT Cuts at: 1563 8445 10749 11122 1563 Size: 6882 2304 373 3063 Fragments arranged by size: 6882 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2195 2426 2551 2831 3569 4264 7997 8418 Size: 563 231 125 280 738 695 3733 421 Cuts at: 8418 8754 10685 12069 12337 1632 Size: 336 1931 1384 268 1917 Fragments arranged by size: 3733 1931 1917 1384 738 695 563 421 336 280 268 231 125 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12425 AflIII A'CryG_T Cuts at: 1203 2322 5366 11521 12498 1203 Size: 1119 3044 6155 977 1327 Fragments arranged by size: 6155 3044 1327 1119 977 AgeI A'CCGG_T Cuts at: 11453 11453 Size: 12622 AhdI GACnn_n'nnGTC Cuts at: 11351 11351 Size: 12622 AleI CACnn'nnGTG Cuts at: 1166 6680 9863 1166 Size: 5514 3183 3925 Fragments arranged by size: 5514 3925 3183 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 6038 9761 1184 Size: 4854 3723 4045 Fragments arranged by size: 4854 4045 3723 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4718 4750 7791 7823 4718 Size: 32 3041 32 9517 Fragments arranged by size: 9517 3041 32 32 AlwNI CAG_nnn'CTG Cuts at: 3236 6953 7249 8763 10365 11937 3236 Size: 3717 296 1514 1602 1572 3921 Fragments arranged by size: 3921 3717 1602 1572 1514 296 ApaI G_GGCC'C Cuts at: 2596 3569 3755 4643 5036 5124 5324 6188 6372 Size: 973 186 888 393 88 200 864 184 Cuts at: 6372 6474 6558 7297 7694 8240 8676 8717 8813 Size: 102 84 739 397 546 436 41 96 Cuts at: 8813 9628 2596 Size: 815 5590 Fragments arranged by size: 5590 973 888 864 815 739 546 436 397 393 200 186 184 102 96 88 84 41 ApaLI G'TGCA_C Cuts at: 3371 6937 7036 7045 9083 10679 11835 3371 Size: 3566 99 9 2038 1596 1156 4158 Fragments arranged by size: 4158 3566 2038 1596 1156 99 9 ApoI r'AATT_y Cuts at: 1493 8753 9931 10157 10309 12418 1493 Size: 7260 1178 226 152 2109 1697 Fragments arranged by size: 7260 2109 1697 1178 226 152 AseI AT'TA_AT Cuts at: 160 1032 11176 160 Size: 872 10144 1606 Fragments arranged by size: 10144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12622 AvaI C'yCGr_G Cuts at: 1870 1943 2022 2336 2623 2735 3047 3312 3442 Size: 73 79 314 287 112 312 265 130 Cuts at: 3442 4524 5038 5339 5396 5507 5560 5681 5850 Size: 1082 514 301 57 111 53 121 169 Cuts at: 5850 6072 6662 7830 7951 8769 9162 9628 9947 Size: 222 590 1168 121 818 393 466 319 Cuts at: 9947 1870 Size: 4545 Fragments arranged by size: 4545 1168 1082 818 590 514 466 393 319 314 312 301 287 265 222 169 130 121 121 112 111 79 73 57 53 AvrII C'CTAG_G Cuts at: 8677 8677 Size: 12622 BamHI G'GATC_C Cuts at: 2529 4864 6760 9952 10319 2529 Size: 2335 1896 3192 367 4832 Fragments arranged by size: 4832 3192 2335 1896 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2600 3716 5050 5285 6043 Size: 325 217 627 813 1116 1334 235 758 Cuts at: 6043 6107 6263 6518 6592 7457 8947 9203 9359 Size: 64 156 255 74 865 1490 256 156 Cuts at: 9359 9451 9916 9943 11398 618 Size: 92 465 27 1455 1842 Fragments arranged by size: 1842 1490 1455 1334 1116 865 813 758 627 465 325 256 255 235 217 156 156 92 74 64 27 BanII G_rGCy'C Cuts at: 1391 2596 2824 2883 3110 3183 3569 3755 4356 Size: 1205 228 59 227 73 386 186 601 Cuts at: 4356 4371 4608 4643 4883 5036 5124 5255 5324 Size: 15 237 35 240 153 88 131 69 Cuts at: 5324 5397 5857 6188 6372 6383 6474 6558 6605 Size: 73 460 331 184 11 91 84 47 Cuts at: 6605 6669 7297 7694 7968 8220 8240 8431 8654 Size: 64 628 397 274 252 20 191 223 Cuts at: 8654 8676 8695 8717 8813 9103 9628 9941 1391 Size: 22 19 22 96 290 525 313 4072 Fragments arranged by size: 4072 1205 628 601 525 460 397 386 331 313 290 274 252 240 237 228 227 223 191 186 184 153 131 96 91 88 84 73 73 69 64 59 47 35 22 22 20 19 15 11 BbeI G_GCGC'C Cuts at: 2604 5289 2604 Size: 2685 9937 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3966 4061 5806 6788 928 Size: 971 2067 95 1745 982 6762 Fragments arranged by size: 6762 2067 1745 982 971 95 BbvCI CC'TCA_GC Cuts at: 3300 3971 4916 8465 3300 Size: 671 945 3549 7457 Fragments arranged by size: 7457 3549 945 671 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2176 2210 2371 2405 3682 3716 4645 Size: 34 239 34 161 34 1277 34 929 Cuts at: 4645 4679 10813 10847 1903 Size: 34 6134 34 3678 Fragments arranged by size: 6134 3678 1277 929 239 161 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10514 11724 10514 Size: 1210 11412 BclI T'GATC_A Cuts at: 1548 2727 6993 8094 1548 Size: 1179 4266 1101 6076 Fragments arranged by size: 6076 4266 1179 1101 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3455 5894 8526 Size: 107 122 71 631 605 1783 2439 2632 Cuts at: 8526 9065 9423 11233 136 Size: 539 358 1810 1525 Fragments arranged by size: 2632 2439 1810 1783 1525 631 605 539 358 122 107 71 BglII A'GATC_T Cuts at: 3219 5592 8826 12617 3219 Size: 2373 3234 3791 3224 Fragments arranged by size: 3791 3234 3224 2373 BlpI GC'TnA_GC Cuts at: 2255 8219 9680 10039 2255 Size: 5964 1461 359 4838 Fragments arranged by size: 5964 4838 1461 359 Bme1580I G_kGCm'C Cuts at: 1790 2596 2623 3290 3375 3396 3569 3719 3755 Size: 806 27 667 85 21 173 150 36 Cuts at: 3755 3827 4215 4438 4643 5036 5055 5124 5157 Size: 72 388 223 205 393 19 69 33 Cuts at: 5157 5324 5388 5727 6027 6048 6188 6372 6474 Size: 167 64 339 300 21 140 184 102 Cuts at: 6474 6543 6558 6918 6941 7040 7049 7176 7297 Size: 69 15 360 23 99 9 127 121 Cuts at: 7297 7694 8019 8240 8606 8676 8717 8813 9087 Size: 397 325 221 366 70 41 96 274 Cuts at: 9087 9364 9419 9613 9628 9921 10683 11839 1790 Size: 277 55 194 15 293 762 1156 2573 Fragments arranged by size: 2573 1156 806 762 667 397 393 388 366 360 339 325 300 293 277 274 223 221 205 194 184 173 167 150 140 127 121 102 99 96 85 72 70 69 69 64 55 41 36 33 27 23 21 21 19 15 15 9 BmgBI CAC'GTC Cuts at: 1318 3376 3820 5881 6001 6499 6838 7035 7078 Size: 2058 444 2061 120 498 339 197 43 Cuts at: 7078 7807 8283 1318 Size: 729 476 5657 Fragments arranged by size: 5657 2061 2058 729 498 476 444 339 197 120 43 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2650 2785 3177 Size: 416 53 83 399 24 1233 135 392 Cuts at: 3177 10353 11311 442 Size: 7176 958 1753 Fragments arranged by size: 7176 1753 1233 958 416 399 392 135 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5614 6205 5614 Size: 591 12031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3197 3326 3458 3782 4461 5534 8291 8744 Size: 1958 129 132 324 679 1073 2757 453 Cuts at: 8744 8760 8805 9125 11282 1239 Size: 16 45 320 2157 2579 Fragments arranged by size: 2757 2579 2157 1958 1073 679 453 324 320 132 129 45 16 Bpu10I CC'TnA_GC Cuts at: 2216 3300 3971 4916 5500 6377 8465 2216 Size: 1084 671 945 584 877 2088 6373 Fragments arranged by size: 6373 2088 1084 945 877 671 584 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2336 4340 9824 10747 11612 11910 2336 Size: 2004 5484 923 865 298 3048 Fragments arranged by size: 5484 3048 2004 923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2918 5915 6134 7245 8140 11285 882 Size: 617 1419 2997 219 1111 895 3145 2219 Fragments arranged by size: 3145 2997 2219 1419 1111 895 617 219 BsaAI yAC'GTr Cuts at: 493 1204 2962 3124 3669 3943 4037 4210 5383 Size: 711 1758 162 545 274 94 173 1173 Cuts at: 5383 5464 6813 6829 7171 7999 8068 8353 8641 Size: 81 1349 16 342 828 69 285 288 Cuts at: 8641 9694 9805 10413 493 Size: 1053 111 608 2702 Fragments arranged by size: 2702 1758 1349 1173 1053 828 711 608 545 342 288 285 274 173 162 111 94 81 69 16 BsaBI GATnn'nnATC Cuts at: 7508 12616 7508 Size: 5108 7514 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2079 2601 2655 Size: 53 83 186 936 531 15 522 54 Cuts at: 2655 3144 3360 3771 4257 4413 4617 4728 4842 Size: 489 216 411 486 156 204 111 114 Cuts at: 4842 5286 5847 6726 6927 8422 8973 9189 9648 Size: 444 561 879 201 1495 551 216 459 Cuts at: 9648 10429 10811 11446 275 Size: 781 382 635 1451 Fragments arranged by size: 1495 1451 936 879 781 635 561 551 531 522 489 486 459 444 411 382 216 216 204 201 186 156 114 111 83 54 53 15 BsaWI w'CCGG_w Cuts at: 5771 5867 11055 11453 11727 11874 12285 5771 Size: 96 5188 398 274 147 411 6108 Fragments arranged by size: 6108 5188 411 398 274 147 96 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2438 2468 4187 4217 4935 Size: 30 165 30 1035 30 1719 30 718 Cuts at: 4935 4965 9371 9401 9455 9485 1178 Size: 30 4406 30 54 30 4315 Fragments arranged by size: 4406 4315 1719 1035 718 165 54 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2489 2492 2501 2753 3002 3593 5993 6998 Size: 1303 3 9 252 249 591 2400 1005 Cuts at: 6998 7976 9122 9800 1186 Size: 978 1146 678 4008 Fragments arranged by size: 4008 2400 1303 1146 1005 978 678 591 252 249 9 3 BseYI C'CCAG_C Cuts at: 2710 3829 5528 5735 6026 6542 7125 7205 7481 Size: 1119 1699 207 291 516 583 80 276 Cuts at: 7481 8240 8708 8813 9319 9615 11825 12321 2710 Size: 759 468 105 506 296 2210 496 3011 Fragments arranged by size: 3011 2210 1699 1119 759 583 516 506 496 468 296 291 276 207 105 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 5064 5972 7244 7481 7699 8305 Size: 573 690 2638 908 1272 237 218 606 Cuts at: 8305 1163 Size: 5480 Fragments arranged by size: 5480 2638 1272 908 690 606 573 237 218 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2590 2976 5619 6240 9072 10072 Size: 275 186 589 386 2643 621 2832 1000 Cuts at: 10072 10833 11861 1540 Size: 761 1028 2301 Fragments arranged by size: 2832 2643 2301 1028 1000 761 621 589 386 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2256 2769 3081 3375 5501 6365 6941 7040 Size: 84 513 312 294 2126 864 576 99 Cuts at: 7040 7049 7170 8037 9087 9888 10683 10768 11839 Size: 9 121 867 1050 801 795 85 1071 Cuts at: 11839 2172 Size: 2955 Fragments arranged by size: 2955 2126 1071 1050 867 864 801 795 576 513 312 294 121 99 85 84 9 BsmI GAATG_Cn' Cuts at: 7779 10133 10226 7779 Size: 2354 93 10175 Fragments arranged by size: 10175 2354 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6980 7904 8018 9545 6980 Size: 924 114 1527 10057 Fragments arranged by size: 10057 1527 924 114 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2256 2596 2623 2769 2824 2883 Size: 399 382 84 340 27 146 55 59 Cuts at: 2883 3081 3110 3183 3290 3375 3396 3569 3719 Size: 198 29 73 107 85 21 173 150 Cuts at: 3719 3755 3827 4215 4356 4371 4438 4608 4643 Size: 36 72 388 141 15 67 170 35 Cuts at: 4643 4883 5036 5055 5124 5157 5255 5324 5388 Size: 240 153 19 69 33 98 69 64 Cuts at: 5388 5397 5501 5727 5857 6027 6048 6188 6365 Size: 9 104 226 130 170 21 140 177 Cuts at: 6365 6372 6383 6474 6543 6558 6605 6669 6918 Size: 7 11 91 69 15 47 64 249 Cuts at: 6918 6941 7040 7049 7170 7176 7297 7694 7968 Size: 23 99 9 121 6 121 397 274 Cuts at: 7968 8019 8037 8220 8240 8431 8606 8654 8676 Size: 51 18 183 20 191 175 48 22 Cuts at: 8676 8695 8717 8813 9087 9103 9364 9419 9613 Size: 19 22 96 274 16 261 55 194 Cuts at: 9613 9628 9888 9921 9941 10683 10768 11839 1391 Size: 15 260 33 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 397 388 382 340 274 274 261 260 249 240 226 198 194 191 183 177 175 173 170 170 153 150 146 141 140 130 121 121 107 104 99 98 96 91 85 85 84 73 72 69 69 69 67 64 64 59 55 55 51 48 47 36 35 33 33 29 27 23 22 22 21 21 20 20 19 19 18 16 15 15 15 11 9 9 7 6 BspEI T'CCGG_A Cuts at: 12285 12285 Size: 12622 BspHI T'CATG_A Cuts at: 2775 4612 6631 10511 12241 2775 Size: 1837 2019 3880 1730 3156 Fragments arranged by size: 3880 3156 2019 1837 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2211 5737 5764 6445 7761 9281 9843 9977 Size: 1367 3526 27 681 1316 1520 562 134 Cuts at: 9977 844 Size: 3489 Fragments arranged by size: 3526 3489 1520 1367 1316 681 562 134 27 BsrBI CCG'CTC Cuts at: 1394 1823 2586 5518 10509 1394 Size: 429 763 2932 4991 3507 Fragments arranged by size: 4991 3507 2932 763 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4192 4495 6449 8796 9352 11117 11291 Size: 1183 2944 303 1954 2347 556 1765 174 Cuts at: 11291 65 Size: 1396 Fragments arranged by size: 2944 2347 1954 1765 1396 1183 556 303 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 3386 4222 5288 9813 11266 11453 Size: 110 279 1297 836 1066 4525 1453 187 Cuts at: 11453 1700 Size: 2869 Fragments arranged by size: 4525 2869 1453 1297 1066 836 279 187 110 BsrGI T'GTAC_A Cuts at: 96 3519 5625 5952 96 Size: 3423 2106 327 6766 Fragments arranged by size: 6766 3423 2106 327 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12622 BssSI C'ACGA_G Cuts at: 4364 7499 9340 9563 10682 11694 4364 Size: 3135 1841 223 1119 1012 5292 Fragments arranged by size: 5292 3135 1841 1119 1012 223 BstAPI GCAn_nnn'nTGC Cuts at: 794 7044 8041 794 Size: 6250 997 5375 Fragments arranged by size: 6250 5375 997 BstBI TT'CG_AA Cuts at: 3702 11426 3702 Size: 7724 4898 BstEII G'GTnAC_C Cuts at: 1184 3553 5137 8290 8512 9370 1184 Size: 2369 1584 3153 222 858 4436 Fragments arranged by size: 4436 3153 2369 1584 858 222 BstXI CCAn_nnnn'nTGG Cuts at: 5029 5441 5645 5786 6481 8089 8248 9215 9658 Size: 412 204 141 695 1608 159 967 443 Cuts at: 9658 9673 5029 Size: 15 7978 Fragments arranged by size: 7978 1608 967 695 443 412 204 159 141 15 BstYI r'GATC_y Cuts at: 1972 2133 2529 3219 4864 5592 6760 6882 7915 Size: 161 396 690 1645 728 1168 122 1033 Cuts at: 7915 8826 9231 9789 9952 10319 10704 10721 12173 Size: 911 405 558 163 367 385 17 1452 Cuts at: 12173 12259 12271 12617 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1645 1452 1168 1033 911 728 690 558 405 396 385 367 346 163 161 122 86 17 12 BstZ17I GTA'TAC Cuts at: 4327 6816 4327 Size: 2489 10133 Bsu36I CC'TnA_GG Cuts at: 5905 7343 7414 9094 9420 5905 Size: 1438 71 1680 326 9107 Fragments arranged by size: 9107 1680 1438 326 71 BtgI C'CryG_G Cuts at: 513 1065 2123 3273 3351 3636 4239 5638 5938 Size: 552 1058 1150 78 285 603 1399 300 Cuts at: 5938 5994 6085 6699 7609 8667 9447 513 Size: 56 91 614 910 1058 780 3688 Fragments arranged by size: 3688 1399 1150 1058 1058 910 780 614 603 552 300 285 91 78 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2179 3082 3178 3837 5958 8214 Size: 683 56 953 903 96 659 2121 2256 Cuts at: 8214 487 Size: 4895 Fragments arranged by size: 4895 2256 2121 953 903 683 659 96 56 BtsI GCAGTG_nn' Cuts at: 3544 4767 5251 7584 7824 10138 10932 10952 3544 Size: 1223 484 2333 240 2314 794 20 5214 Fragments arranged by size: 5214 2333 2314 1223 794 484 240 20 ClaI AT'CG_AT Cuts at: 1554 6714 9072 1554 Size: 5160 2358 5104 Fragments arranged by size: 5160 5104 2358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3196 3231 4090 4125 6853 6888 529 Size: 35 2632 35 859 35 2728 35 6263 Fragments arranged by size: 6263 2728 2632 859 35 35 35 35 DraI TTT'AAA Cuts at: 9926 9926 Size: 12622 DraIII CAC_nnn'GTG Cuts at: 3140 3370 3723 5995 7044 8041 3140 Size: 230 353 2272 1049 997 7721 Fragments arranged by size: 7721 2272 1049 997 353 230 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2785 6346 8897 11629 809 Size: 507 1469 3561 2551 2732 1802 Fragments arranged by size: 3561 2732 2551 1802 1469 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2587 2938 Size: 54 1425 50 275 18 261 496 351 Cuts at: 2938 2973 3088 3427 3463 3911 4065 4402 5616 Size: 35 115 339 36 448 154 337 1214 Cuts at: 5616 5713 5895 6292 7279 7576 7680 7932 7993 Size: 97 182 397 987 297 104 252 61 Cuts at: 7993 8059 8070 8439 8793 9663 10069 10958 12587 Size: 66 11 369 354 870 406 889 1629 Cuts at: 12587 8 Size: 43 Fragments arranged by size: 1629 1425 1214 987 889 870 496 448 406 397 369 354 351 339 337 297 275 261 252 182 154 115 104 97 66 61 54 50 43 36 35 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2587 2973 5616 10069 1537 Size: 275 775 386 2643 4453 4090 Fragments arranged by size: 4453 4090 2643 775 386 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5426 6582 9698 10552 1353 Size: 4073 1156 3116 854 3423 Fragments arranged by size: 4073 3423 3116 1156 854 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2126 2291 2714 3956 11209 11583 Size: 1064 138 537 165 423 1242 7253 374 Cuts at: 11583 11729 387 Size: 146 1280 Fragments arranged by size: 7253 1280 1242 1064 537 423 374 165 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2426 2551 2831 3197 3326 3458 Size: 393 563 231 125 280 366 129 132 Cuts at: 3458 3569 3782 4264 4461 5534 7997 8291 8418 Size: 111 213 482 197 1073 2463 294 127 Cuts at: 8418 8744 8754 8760 8805 9125 10685 11282 12069 Size: 326 10 6 45 320 1560 597 787 Cuts at: 12069 12337 1239 Size: 268 1524 Fragments arranged by size: 2463 1560 1524 1073 787 597 563 482 393 366 326 320 294 280 268 231 213 197 132 129 127 125 111 45 10 6 EcoNI CCTnn'n_nnAGG Cuts at: 2111 9839 2111 Size: 7728 4894 EcoO109I rG'GnC_Cy Cuts at: 2104 2593 2968 3167 3268 3469 3565 3566 3598 Size: 489 375 199 101 201 96 1 32 Cuts at: 3598 3751 3752 4117 4451 4639 4640 5033 5120 Size: 153 1 365 334 188 1 393 87 Cuts at: 5120 5121 5320 5321 5504 5685 6184 6185 6322 Size: 1 199 1 183 181 499 1 137 Cuts at: 6322 6470 6554 6555 6736 7294 7305 7347 7669 Size: 148 84 1 181 558 11 42 322 Cuts at: 7669 7690 8146 8236 8237 8266 8672 8673 8713 Size: 21 456 90 1 29 406 1 40 Cuts at: 8713 8714 8809 8810 9262 9533 9624 9625 9632 Size: 1 95 1 452 271 91 1 7 Cuts at: 9632 9670 9774 2104 Size: 38 104 4952 Fragments arranged by size: 4952 558 499 489 456 452 406 393 375 365 334 322 271 201 199 199 188 183 181 181 153 148 137 104 101 96 95 91 90 87 84 42 40 38 32 29 21 11 7 1 1 1 1 1 1 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 8753 9931 10309 8753 Size: 1178 378 11066 Fragments arranged by size: 11066 1178 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3127 9727 3127 Size: 6600 6022 FspI TGC'GCA Cuts at: 11128 11128 Size: 12622 HaeII r_GCGC'y Cuts at: 1678 2336 2604 3534 5289 11769 1678 Size: 658 268 930 1755 6480 2531 Fragments arranged by size: 6480 2531 1755 930 658 268 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2309 2341 2630 2662 2753 Size: 32 135 32 737 32 289 32 91 Cuts at: 2753 2785 3046 3078 6745 6777 7202 7234 7679 Size: 32 261 32 3667 32 425 32 445 Cuts at: 7679 7711 9371 9403 9557 9589 11263 11295 11337 Size: 32 1660 32 154 32 1674 32 42 Cuts at: 11337 11369 1373 Size: 32 2626 Fragments arranged by size: 3667 2626 1674 1660 737 445 425 289 261 154 135 91 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2068 4072 7570 7645 9022 9966 Size: 988 281 130 2004 3498 75 1377 944 Cuts at: 9966 10212 669 Size: 246 3079 Fragments arranged by size: 3498 3079 2004 1377 988 944 281 246 130 75 HindIII A'AGCT_T Cuts at: 748 748 Size: 12622 HpaI GTT'AAC Cuts at: 10212 10212 Size: 12622 KasI G'GCGC_C Cuts at: 2600 5285 2600 Size: 2685 9937 KpnI G_GTAC'C Cuts at: 6596 9947 6596 Size: 3351 9271 MfeI C'AATT_G Cuts at: 10221 10221 Size: 12622 MluI A'CGCG_T Cuts at: 12498 12498 Size: 12622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 8085 11736 11920 1305 Size: 921 5859 3651 184 2007 Fragments arranged by size: 5859 3651 2007 921 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2940 3090 3429 4404 5897 Size: 54 1425 343 1108 150 339 975 1493 Cuts at: 5897 6294 7578 7682 7995 8061 8072 8441 8795 Size: 397 1284 104 313 66 11 369 354 Cuts at: 8795 9665 10 Size: 870 2967 Fragments arranged by size: 2967 1493 1425 1284 1108 975 870 397 369 354 343 339 313 150 104 66 54 11 MslI CAynn'nnrTG Cuts at: 518 1166 2456 2462 4238 4547 5643 6680 7148 Size: 648 1290 6 1776 309 1096 1037 468 Cuts at: 7148 7856 8087 9863 10580 10939 11098 518 Size: 708 231 1776 717 359 159 2042 Fragments arranged by size: 2042 1776 1776 1290 1096 1037 717 708 648 468 359 309 231 159 6 MspA1I CmG'CkG Cuts at: 1606 2290 2551 2616 3304 3333 3506 4200 5048 Size: 684 261 65 688 29 173 694 848 Cuts at: 5048 5528 6231 7249 7713 8594 9323 10038 10715 Size: 480 703 1018 464 881 729 715 677 Cuts at: 10715 11863 12108 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1018 881 848 729 715 703 694 688 684 677 480 464 261 245 173 65 29 NaeI GCC'GGC Cuts at: 2091 3388 5290 9815 2091 Size: 1297 1902 4525 4898 Fragments arranged by size: 4898 4525 1902 1297 NarI GG'CG_CC Cuts at: 2601 5286 2601 Size: 2685 9937 NcoI C'CATG_G Cuts at: 513 1065 3273 3351 3636 4239 7609 9447 513 Size: 552 2208 78 285 603 3370 1838 3688 Fragments arranged by size: 3688 3370 2208 1838 603 552 285 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 12622 NgoMIV G'CCGG_C Cuts at: 2089 3386 5288 9813 2089 Size: 1297 1902 4525 4898 Fragments arranged by size: 4898 4525 1902 1297 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12622 NotI GC'GGCC_GC Cuts at: 10069 10069 Size: 12622 NspI r_CATG'y Cuts at: 1132 1229 5370 5953 7528 7740 8048 9748 9980 Size: 97 4141 583 1575 212 308 1700 232 Cuts at: 9980 11525 1132 Size: 1545 2229 Fragments arranged by size: 4141 2229 1700 1575 1545 583 308 232 212 97 PasI CC'CwG_GG Cuts at: 1348 2596 3755 7273 1348 Size: 1248 1159 3518 6697 Fragments arranged by size: 6697 3518 1248 1159 PciI A'CATG_T Cuts at: 5366 11521 5366 Size: 6155 6467 PflMI CCAn_nnn'nTGG Cuts at: 1108 3669 8068 1108 Size: 2561 4399 5662 Fragments arranged by size: 5662 4399 2561 PfoI T'CCnGG_A Cuts at: 2343 3264 4137 5631 8493 8877 2343 Size: 921 873 1494 2862 384 6088 Fragments arranged by size: 6088 2862 1494 921 873 384 PmeI GTTT'AAAC Cuts at: 9926 9926 Size: 12622 PmlI CAC'GTG Cuts at: 3669 3943 4037 5464 7171 7999 8068 8353 8641 Size: 274 94 1427 1707 828 69 285 288 Cuts at: 8641 9805 3669 Size: 1164 6486 Fragments arranged by size: 6486 1707 1427 1164 828 288 285 274 94 69 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 10691 10723 12204 12236 10691 Size: 32 1481 32 11077 Fragments arranged by size: 11077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2968 3167 3268 3598 6322 8266 9262 9632 Size: 864 199 101 330 2724 1944 996 370 Cuts at: 9632 9774 2104 Size: 142 4952 Fragments arranged by size: 4952 2724 1944 996 864 370 330 199 142 101 PshAI GACnn'nnGTC Cuts at: 1613 6015 1613 Size: 4402 8220 PsiI TTA'TAA Cuts at: 10192 10192 Size: 12622 PspOMI G'GGCC_C Cuts at: 2592 3565 3751 4639 5032 5120 5320 6184 6368 Size: 973 186 888 393 88 200 864 184 Cuts at: 6368 6470 6554 7293 7690 8236 8672 8713 8809 Size: 102 84 739 397 546 436 41 96 Cuts at: 8809 9624 2592 Size: 815 5590 Fragments arranged by size: 5590 973 888 864 815 739 546 436 397 393 200 186 184 102 96 88 84 41 PspXI vC'TCGA_Gb Cuts at: 2336 5339 5560 2336 Size: 3003 221 9398 Fragments arranged by size: 9398 3003 221 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 8555 8587 8555 Size: 32 12590 PstI C_TGCA'G Cuts at: 830 2208 3338 5238 5748 8179 9728 9974 830 Size: 1378 1130 1900 510 2431 1549 246 3478 Fragments arranged by size: 3478 2431 1900 1549 1378 1130 510 246 PvuI CG_AT'CG Cuts at: 2001 9072 2001 Size: 7071 5551 PvuII CAG'CTG Cuts at: 2551 2616 3304 3506 4200 6231 7249 7713 9323 Size: 65 688 202 694 2031 1018 464 1610 Cuts at: 9323 2551 Size: 5850 Fragments arranged by size: 5850 2031 1610 1018 694 688 464 202 65 SalI G'TCGA_C Cuts at: 1936 2066 9964 1936 Size: 130 7898 4594 Fragments arranged by size: 7898 4594 130 SanDI GG'GwC_CC Cuts at: 9262 9262 Size: 12622 SapI GCTCTTCn'nnn_ Cuts at: 9698 9698 Size: 12622 SbfI CC_TGCA'GG Cuts at: 9974 9974 Size: 12622 ScaI AGT'ACT Cuts at: 6573 10870 12582 6573 Size: 4297 1712 6613 Fragments arranged by size: 6613 4297 1712 SexAI A'CCwGG_T Cuts at: 8037 8037 Size: 12622 SfcI C'TryA_G Cuts at: 826 1045 2204 3334 5234 5744 6177 6268 6559 Size: 219 1159 1130 1900 510 433 91 291 Cuts at: 6559 7774 8175 9724 9820 9970 11105 11786 11977 Size: 1215 401 1549 96 150 1135 681 191 Cuts at: 11977 826 Size: 1471 Fragments arranged by size: 1900 1549 1471 1215 1159 1135 1130 681 510 433 401 291 219 191 150 96 91 SfiI GGCCn_nnn'nGGCC Cuts at: 5894 5894 Size: 12622 SfoI GGC'GCC Cuts at: 2602 5287 2602 Size: 2685 9937 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12622 SmaI CCC'GGG Cuts at: 1872 2625 3314 4526 5398 5509 5683 5852 6074 Size: 753 689 1212 872 111 174 169 222 Cuts at: 6074 6664 8771 9630 9949 1872 Size: 590 2107 859 319 4545 Fragments arranged by size: 4545 2107 1212 872 859 753 689 590 319 222 174 169 111 SmlI C'TyrA_G Cuts at: 820 1017 1943 2315 2336 4355 5339 5560 9839 Size: 197 926 372 21 2019 984 221 4279 Cuts at: 9839 10726 11627 11889 820 Size: 887 901 262 1553 Fragments arranged by size: 4279 2019 1553 984 926 901 887 372 262 221 197 21 SnaBI TAC'GTA Cuts at: 493 9694 10413 493 Size: 9201 719 2702 Fragments arranged by size: 9201 2702 719 SpeI A'CTAG_T Cuts at: 152 152 Size: 12622 SphI G_CATG'C Cuts at: 1132 9980 1132 Size: 8848 3774 SrfI GCCC'GGGC Cuts at: 5852 5852 Size: 12622 SspI AAT'ATT Cuts at: 5 52 10546 5 Size: 47 10494 2081 Fragments arranged by size: 10494 2081 47 StuI AGG'CCT Cuts at: 1517 5073 6480 8790 9689 1517 Size: 3556 1407 2310 899 4450 Fragments arranged by size: 4450 3556 2310 1407 899 StyI C'CwwG_G Cuts at: 513 1065 2799 3163 3273 3351 3480 3570 3636 Size: 552 1734 364 110 78 129 90 66 Cuts at: 3636 4239 4749 5280 6351 7609 7650 8019 8677 Size: 603 510 531 1071 1258 41 369 658 Cuts at: 8677 9060 9078 9390 9447 9597 9666 9770 10061 Size: 383 18 312 57 150 69 104 291 Cuts at: 10061 513 Size: 3074 Fragments arranged by size: 3074 1734 1258 1071 658 603 552 531 510 383 369 364 312 291 150 129 110 104 90 78 69 66 57 41 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4032 4697 5447 6068 6226 6536 7304 Size: 1255 2452 665 750 621 158 310 768 Cuts at: 7304 7495 7622 9221 10649 10666 10819 11004 11449 Size: 191 127 1599 1428 17 153 185 445 Cuts at: 11449 325 Size: 1498 Fragments arranged by size: 2452 1599 1498 1428 1255 768 750 665 621 445 310 191 185 158 153 127 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3519 4515 4812 Size: 274 80 33 51 1443 1542 996 297 Cuts at: 4812 5625 5952 5964 6243 6571 7260 7791 8625 Size: 813 327 12 279 328 689 531 834 Cuts at: 8625 9855 10868 12580 96 Size: 1230 1013 1712 138 Fragments arranged by size: 1712 1542 1443 1230 1013 996 834 813 689 531 328 327 297 279 274 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2108 2140 4113 4145 4935 4967 8267 8299 12582 Size: 32 1973 32 790 32 3300 32 4283 Cuts at: 12582 12614 2108 Size: 32 2116 Fragments arranged by size: 4283 3300 2116 1973 790 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 7358 1316 Size: 6042 6580 XbaI T'CTAG_A Cuts at: 9958 9958 Size: 12622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2890 6481 6697 8108 8354 8642 9397 9658 2890 Size: 3591 216 1411 246 288 755 261 5854 Fragments arranged by size: 5854 3591 1411 755 288 261 246 216 XhoI C'TCGA_G Cuts at: 1943 2336 5339 5560 1943 Size: 393 3003 221 9005 Fragments arranged by size: 9005 3003 393 221 XmaI C'CCGG_G Cuts at: 1870 2623 3312 4524 5396 5507 5681 5850 6072 Size: 753 689 1212 872 111 174 169 222 Cuts at: 6072 6662 8769 9628 9947 1870 Size: 590 2107 859 319 4545 Fragments arranged by size: 4545 2107 1212 872 859 753 689 590 319 222 174 169 111 XmnI GAAnn'nnTTC Cuts at: 2147 8925 10751 2147 Size: 6778 1826 4018 Fragments arranged by size: 6778 4018 1826 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4414 4729 9190 10430 Size: 53 83 186 936 2880 315 4461 1240 Cuts at: 10430 276 Size: 2468 Fragments arranged by size: 4461 2880 2468 1240 936 315 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI EcoICRI EcoRV FseI FspAI NruI NsiI PacI RsrII SacI SacII SwaI