(Circular) (Six-base) MAPSORT of: pFN21AF0293.seq Check: 7699 from: 1 to: 7759 pFN21AF0293 7759 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2300 3999 4373 4374 2300 Size: 1699 374 1 5685 Fragments arranged by size: 5685 1699 374 1 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3576 5569 278 Size: 53 83 186 936 2040 1993 2468 Fragments arranged by size: 2468 2040 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2685 5102 1937 Size: 748 2417 4594 Fragments arranged by size: 4594 2417 748 Acc65I G'GTAC_C Cuts at: 2199 5080 2199 Size: 2881 4878 AclI AA'CG_TT Cuts at: 1563 5886 6259 1563 Size: 4323 373 3063 Fragments arranged by size: 4323 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2779 2823 3308 4407 5822 7206 7474 1632 Size: 1147 44 485 1099 1415 1384 268 1917 Fragments arranged by size: 1917 1415 1384 1147 1099 485 268 44 AfeI AGC'GCT Cuts at: 4021 4021 Size: 7759 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7562 AflIII A'CryG_T Cuts at: 1203 6658 7635 1203 Size: 5455 977 1327 Fragments arranged by size: 5455 1327 977 AgeI A'CCGG_T Cuts at: 2277 6590 2277 Size: 4313 3446 AhdI GACnn_n'nnGTC Cuts at: 4522 6488 4522 Size: 1966 5793 AleI CACnn'nnGTG Cuts at: 1166 2432 2592 3362 3707 1166 Size: 1266 160 770 345 5218 Fragments arranged by size: 5218 1266 770 345 160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4396 1184 Size: 3212 4547 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3764 3796 3764 Size: 32 7727 AlwNI CAG_nnn'CTG Cuts at: 2167 3091 3463 5502 7074 2167 Size: 924 372 2039 1572 2852 Fragments arranged by size: 2852 2039 1572 924 372 ApaI G_GGCC'C Cuts at: 4396 4468 4513 4669 4396 Size: 72 45 156 7486 Fragments arranged by size: 7486 156 72 45 ApaLI G'TGCA_C Cuts at: 2726 5816 6972 2726 Size: 3090 1156 3513 Fragments arranged by size: 3513 3090 1156 ApoI r'AATT_y Cuts at: 1493 5068 5294 5446 7555 1493 Size: 3575 226 152 2109 1697 Fragments arranged by size: 3575 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6313 160 Size: 872 5281 1606 Fragments arranged by size: 5281 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7759 AvaI C'yCGr_G Cuts at: 1870 1943 2571 2958 3001 3057 3135 4512 5084 Size: 73 628 387 43 56 78 1377 572 Cuts at: 5084 1870 Size: 4545 Fragments arranged by size: 4545 1377 628 572 387 78 73 56 43 BamHI G'GATC_C Cuts at: 3145 5089 5456 3145 Size: 1944 367 5448 Fragments arranged by size: 5448 1944 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2199 3431 4172 4968 5080 Size: 325 217 627 412 1232 741 796 112 Cuts at: 5080 6535 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1232 796 741 627 412 325 217 112 BanII G_rGCy'C Cuts at: 1391 2217 3792 4001 4126 4255 4396 4468 4513 Size: 826 1575 209 125 129 141 72 45 Cuts at: 4513 4669 4989 5010 5078 1391 Size: 156 320 21 68 4072 Fragments arranged by size: 4072 1575 826 320 209 156 141 129 125 72 68 45 21 BbeI G_GCGC'C Cuts at: 4972 4972 Size: 7759 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2333 928 Size: 971 434 6354 Fragments arranged by size: 6354 971 434 BbvCI CC'TCA_GC Cuts at: 2391 3042 4202 2391 Size: 651 1160 5948 Fragments arranged by size: 5948 1160 651 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4460 4494 5950 5984 1903 Size: 34 2523 34 1456 34 3678 Fragments arranged by size: 3678 2523 1456 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5651 6861 5651 Size: 1210 6549 BclI T'GATC_A Cuts at: 1548 1548 Size: 7759 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2264 2490 2918 Size: 107 122 71 631 605 592 226 428 Cuts at: 2918 3688 4451 4463 6370 136 Size: 770 763 12 1907 1525 Fragments arranged by size: 1907 1525 770 763 631 605 592 428 226 122 107 71 12 BglII A'GATC_T Cuts at: 7754 7754 Size: 7759 BlpI GC'TnA_GC Cuts at: 2940 4263 4566 4760 5176 2940 Size: 1323 303 194 416 5523 Fragments arranged by size: 5523 1323 416 303 194 Bme1580I G_kGCm'C Cuts at: 1790 2730 4175 4396 4468 4513 4669 4740 5820 Size: 940 1445 221 72 45 156 71 1080 Cuts at: 5820 6976 1790 Size: 1156 2573 Fragments arranged by size: 2573 1445 1156 1080 940 221 156 72 71 45 BmgBI CAC'GTC Cuts at: 1318 2413 4054 1318 Size: 1095 1641 5023 Fragments arranged by size: 5023 1641 1095 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4498 5490 6448 Size: 416 53 83 399 24 3081 992 958 Cuts at: 6448 442 Size: 1753 Fragments arranged by size: 3081 1753 992 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7759 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2729 3635 4145 2729 Size: 906 510 6343 Fragments arranged by size: 6343 906 510 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2459 2464 2582 3908 4049 4130 4940 6419 Size: 1220 5 118 1326 141 81 810 1479 Cuts at: 6419 1239 Size: 2579 Fragments arranged by size: 2579 1479 1326 1220 810 141 118 81 5 Bpu10I CC'TnA_GC Cuts at: 2096 2391 3042 3950 4202 2096 Size: 295 651 908 252 5653 Fragments arranged by size: 5653 908 651 295 252 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2405 2564 4763 5884 6749 7047 2405 Size: 159 2199 1121 865 298 3117 Fragments arranged by size: 3117 2199 1121 865 298 159 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4773 6422 882 Size: 617 3274 1649 2219 Fragments arranged by size: 3274 2219 1649 617 BsaAI yAC'GTr Cuts at: 493 1204 2590 3579 5550 493 Size: 711 1386 989 1971 2702 Fragments arranged by size: 2702 1971 1386 989 711 BsaBI GATnn'nnATC Cuts at: 7753 7753 Size: 7759 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3573 4969 5566 5948 Size: 53 83 186 936 2040 1396 597 382 Cuts at: 5948 6583 275 Size: 635 1451 Fragments arranged by size: 2040 1451 1396 936 635 597 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2277 3549 4233 4656 6192 6590 6864 7011 7422 Size: 1272 684 423 1536 398 274 147 411 Cuts at: 7422 2277 Size: 2614 Fragments arranged by size: 2614 1536 1272 684 423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3764 3794 1178 Size: 30 165 30 2361 30 5143 Fragments arranged by size: 5143 2361 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 2930 4791 1186 Size: 1705 39 1861 4154 Fragments arranged by size: 4154 1861 1705 39 BseYI C'CCAG_C Cuts at: 3833 3994 5051 6962 7458 3833 Size: 161 1057 1911 496 4134 Fragments arranged by size: 4134 1911 1057 496 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2645 4781 4817 1163 Size: 573 909 2136 36 4105 Fragments arranged by size: 4105 2136 909 573 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2535 4847 5209 5970 6998 1540 Size: 275 186 534 2312 362 761 1028 2301 Fragments arranged by size: 2312 2301 1028 761 534 362 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 2730 2973 4614 5820 5905 6976 2217 Size: 513 243 1641 1206 85 1071 3000 Fragments arranged by size: 3000 1641 1206 1071 513 243 85 BsmI GAATG_Cn' Cuts at: 5270 5363 5270 Size: 93 7666 BsmBI CGTCTCn'nnnn_ Cuts at: 2864 4936 2864 Size: 2072 5687 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2730 2973 3792 4001 4126 4175 Size: 399 427 513 243 819 209 125 49 Cuts at: 4175 4255 4396 4468 4513 4614 4669 4740 4989 Size: 80 141 72 45 101 55 71 249 Cuts at: 4989 5010 5078 5820 5905 6976 1391 Size: 21 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 819 742 513 427 399 249 243 209 141 125 101 85 80 72 71 68 55 49 45 21 BspEI T'CCGG_A Cuts at: 3549 4233 4656 7422 3549 Size: 684 423 2766 3886 Fragments arranged by size: 3886 2766 684 423 BspHI T'CATG_A Cuts at: 2079 5648 7378 2079 Size: 3569 1730 2460 Fragments arranged by size: 3569 2460 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 2300 3999 4373 4374 5114 844 Size: 1253 203 1699 374 1 740 3489 Fragments arranged by size: 3489 1699 1253 740 374 203 1 BsrBI CCG'CTC Cuts at: 1394 1823 2224 2976 3379 4086 4839 5646 1394 Size: 429 401 752 403 707 753 807 3507 Fragments arranged by size: 3507 807 753 752 707 429 403 401 BsrDI GCAATG_nn' Cuts at: 65 1248 2274 2458 6254 6428 65 Size: 1183 1026 184 3796 174 1396 Fragments arranged by size: 3796 1396 1183 1026 184 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2277 4710 6403 6590 1700 Size: 110 467 2433 1693 187 2869 Fragments arranged by size: 2869 2433 1693 467 187 110 BsrGI T'GTAC_A Cuts at: 96 3645 96 Size: 3549 4210 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7759 BssSI C'ACGA_G Cuts at: 2966 4820 5819 6831 2966 Size: 1854 999 1012 3894 Fragments arranged by size: 3894 1854 1012 999 BstAPI GCAn_nnn'nTGC Cuts at: 794 2350 2659 4042 794 Size: 1556 309 1383 4511 Fragments arranged by size: 4511 1556 1383 309 BstBI TT'CG_AA Cuts at: 6563 6563 Size: 7759 BstEII G'GTnAC_C Cuts at: 1184 3709 1184 Size: 2525 5234 BstXI CCAn_nnnn'nTGG Cuts at: 3364 3364 Size: 7759 BstYI r'GATC_y Cuts at: 1972 3145 3367 4653 5089 5456 5841 5858 7310 Size: 1173 222 1286 436 367 385 17 1452 Cuts at: 7310 7396 7408 7754 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1286 1173 436 385 367 346 222 86 17 12 BstZ17I GTA'TAC Cuts at: 2686 2686 Size: 7759 Bsu36I CC'TnA_GG Cuts at: 2184 2184 Size: 7759 BtgI C'CryG_G Cuts at: 513 1065 2004 2067 3388 3528 3585 3753 4458 Size: 552 939 63 1321 140 57 168 705 Cuts at: 4458 4804 4980 513 Size: 346 176 3292 Fragments arranged by size: 3292 1321 939 705 552 346 176 168 140 63 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2176 2344 2807 4940 487 Size: 683 56 950 168 463 2133 3306 Fragments arranged by size: 3306 2133 950 683 463 168 56 BtsI GCAGTG_nn' Cuts at: 2641 2772 2913 3033 3084 4894 5275 6069 6089 Size: 131 141 120 51 1810 381 794 20 Cuts at: 6089 2641 Size: 4311 Fragments arranged by size: 4311 1810 794 381 141 131 120 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7759 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7724 DraI TTT'AAA Cuts at: 5063 5063 Size: 7759 DraIII CAC_nnn'GTG Cuts at: 4246 4246 Size: 7759 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6766 809 Size: 507 5450 1802 Fragments arranged by size: 5450 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2225 2247 2910 Size: 54 1425 50 275 18 395 22 663 Cuts at: 2910 2949 3399 3538 3680 4063 4325 4452 4807 Size: 39 450 139 142 383 262 127 355 Cuts at: 4807 4844 4887 5206 6095 7724 8 Size: 37 43 319 889 1629 43 Fragments arranged by size: 1629 1425 889 663 450 395 383 355 319 275 262 142 139 127 54 50 43 43 39 37 22 18 EagI C'GGCC_G Cuts at: 1537 1812 4844 5206 1537 Size: 275 3032 362 4090 Fragments arranged by size: 4090 3032 362 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2799 3297 3626 3746 4382 5689 1353 Size: 1446 498 329 120 636 1307 3423 Fragments arranged by size: 3423 1446 1307 636 498 329 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6346 6720 6866 387 Size: 1064 138 4757 374 146 1280 Fragments arranged by size: 4757 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2215 Size: 7759 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2459 2464 2582 2779 2823 3308 3908 Size: 393 827 5 118 197 44 485 600 Cuts at: 3908 4049 4130 4407 4940 5822 6419 7206 7474 Size: 141 81 277 533 882 597 787 268 Cuts at: 7474 1239 Size: 1524 Fragments arranged by size: 1524 882 827 787 600 597 533 485 393 277 268 197 141 118 81 44 5 EcoNI CCTnn'n_nnAGG Cuts at: 3290 3623 4180 3290 Size: 333 557 6869 Fragments arranged by size: 6869 557 333 EcoO109I rG'GnC_Cy Cuts at: 2021 2527 2575 2899 2962 4464 4465 4666 2021 Size: 506 48 324 63 1502 1 201 5114 Fragments arranged by size: 5114 1502 506 324 201 63 48 1 EcoRI G'AATT_C Cuts at: 5068 5446 5068 Size: 378 7381 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3808 3808 Size: 7759 FspI TGC'GCA Cuts at: 6265 6265 Size: 7759 HaeII r_GCGC'y Cuts at: 1678 3721 4023 4192 4972 6906 1678 Size: 2043 302 169 780 1934 2531 Fragments arranged by size: 2531 2043 1934 780 302 169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2529 2561 2798 2830 3933 Size: 32 135 32 957 32 237 32 1103 Cuts at: 3933 3965 4508 4540 6400 6432 6474 6506 1373 Size: 32 543 32 1860 32 42 32 2626 Fragments arranged by size: 2626 1860 1103 957 543 237 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3217 5103 5349 669 Size: 988 281 1279 1886 246 3079 Fragments arranged by size: 3079 1886 1279 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7759 HpaI GTT'AAC Cuts at: 5349 5349 Size: 7759 KasI G'GCGC_C Cuts at: 4968 4968 Size: 7759 KpnI G_GTAC'C Cuts at: 2203 5084 2203 Size: 2881 4878 MfeI C'AATT_G Cuts at: 2621 5358 2621 Size: 2737 5022 MluI A'CGCG_T Cuts at: 7635 7635 Size: 7759 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4026 4959 6873 7057 1305 Size: 2721 933 1914 184 2007 Fragments arranged by size: 2721 2007 1914 933 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 4065 10 Size: 54 1425 343 417 1816 3704 Fragments arranged by size: 3704 1816 1425 417 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2238 2432 2592 2723 3179 3362 3707 Size: 648 1072 194 160 131 456 183 345 Cuts at: 3707 4142 4220 4979 5717 6076 6235 518 Size: 435 78 759 738 359 159 2042 Fragments arranged by size: 2042 1072 759 738 648 456 435 359 345 194 183 160 159 131 78 MspA1I CmG'CkG Cuts at: 1606 2650 3041 3088 3804 4273 4330 4402 4773 Size: 1044 391 47 716 469 57 72 371 Cuts at: 4773 4786 4806 5019 5175 5852 7000 7245 1606 Size: 13 20 213 156 677 1148 245 2120 Fragments arranged by size: 2120 1148 1044 716 677 469 391 371 245 213 156 72 57 47 20 13 NarI GG'CG_CC Cuts at: 4969 4969 Size: 7759 NcoI C'CATG_G Cuts at: 513 1065 2004 2067 3528 3753 4458 513 Size: 552 939 63 1461 225 705 3814 Fragments arranged by size: 3814 1461 939 705 552 225 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 7759 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7759 NotI GC'GGCC_GC Cuts at: 5206 5206 Size: 7759 NspI r_CATG'y Cuts at: 1132 1229 4432 5117 6662 1132 Size: 97 3203 685 1545 2229 Fragments arranged by size: 3203 2229 1545 685 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7759 PciI A'CATG_T Cuts at: 6658 6658 Size: 7759 PflMI CCAn_nnn'nTGG Cuts at: 1108 3994 4789 1108 Size: 2886 795 4078 Fragments arranged by size: 4078 2886 795 PfoI T'CCnGG_A Cuts at: 3624 4026 4356 3624 Size: 402 330 7027 Fragments arranged by size: 7027 402 330 PmeI GTTT'AAAC Cuts at: 5063 5063 Size: 7759 PmlI CAC'GTG Cuts at: 2590 2590 Size: 7759 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5828 5860 7341 7373 3764 Size: 32 2032 32 1481 32 4150 Fragments arranged by size: 4150 2032 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 2575 2962 2527 Size: 48 387 7324 Fragments arranged by size: 7324 387 48 PshAI GACnn'nnGTC Cuts at: 1613 4100 1613 Size: 2487 5272 PsiI TTA'TAA Cuts at: 5329 5329 Size: 7759 PspOMI G'GGCC_C Cuts at: 4392 4464 4509 4665 4392 Size: 72 45 156 7486 Fragments arranged by size: 7486 156 72 45 PstI C_TGCA'G Cuts at: 830 2094 2892 3040 3264 3422 3459 4047 4272 Size: 1264 798 148 224 158 37 588 225 Cuts at: 4272 4350 4385 5111 830 Size: 78 35 726 3478 Fragments arranged by size: 3478 1264 798 726 588 225 224 158 148 78 37 35 PvuI CG_AT'CG Cuts at: 2001 2535 2001 Size: 534 7225 PvuII CAG'CTG Cuts at: 2650 3041 4402 4786 2650 Size: 391 1361 384 5623 Fragments arranged by size: 5623 1361 391 384 SacI G_AGCT'C Cuts at: 2217 2217 Size: 7759 SacII CC_GC'GG Cuts at: 4807 4807 Size: 7759 SalI G'TCGA_C Cuts at: 1936 5101 1936 Size: 3165 4594 SanDI GG'GwC_CC Cuts at: 2527 2527 Size: 7759 SbfI CC_TGCA'GG Cuts at: 5111 5111 Size: 7759 ScaI AGT'ACT Cuts at: 6007 7719 6007 Size: 1712 6047 SexAI A'CCwGG_T Cuts at: 3864 3864 Size: 7759 SfcI C'TryA_G Cuts at: 826 1045 2090 2888 3036 3260 3418 3455 4043 Size: 219 1045 798 148 224 158 37 588 Cuts at: 4043 4268 4346 4381 5107 6242 6923 7114 826 Size: 225 78 35 726 1135 681 191 1471 Fragments arranged by size: 1471 1135 1045 798 726 681 588 225 224 219 191 158 148 78 37 35 SfiI GGCCn_nnn'nGGCC Cuts at: 4451 4451 Size: 7759 SfoI GGC'GCC Cuts at: 4970 4970 Size: 7759 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7759 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7759 SmaI CCC'GGG Cuts at: 1872 2960 3059 3137 5086 1872 Size: 1088 99 78 1949 4545 Fragments arranged by size: 4545 1949 1088 99 78 SmlI C'TyrA_G Cuts at: 820 1017 1943 2420 2579 4742 5863 6764 7026 Size: 197 926 477 159 2163 1121 901 262 Cuts at: 7026 820 Size: 1553 Fragments arranged by size: 2163 1553 1121 926 901 477 262 197 159 SnaBI TAC'GTA Cuts at: 493 5550 493 Size: 5057 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7759 SphI G_CATG'C Cuts at: 1132 5117 1132 Size: 3985 3774 SspI AAT'ATT Cuts at: 5 52 5683 5 Size: 47 5631 2081 Fragments arranged by size: 5631 2081 47 StuI AGG'CCT Cuts at: 1517 2444 3416 1517 Size: 927 972 5860 Fragments arranged by size: 5860 972 927 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 2736 3072 3528 3753 3783 Size: 552 939 63 669 336 456 225 30 Cuts at: 3783 4176 4446 4458 5198 513 Size: 393 270 12 740 3074 Fragments arranged by size: 3074 939 740 669 552 456 393 336 270 225 63 30 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2582 2786 3091 3459 5786 5803 5956 Size: 1255 1002 204 305 368 2327 17 153 Cuts at: 5956 6141 6586 325 Size: 185 445 1498 Fragments arranged by size: 2327 1498 1255 1002 445 368 305 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 2496 3645 Size: 274 80 33 51 1443 378 141 1149 Cuts at: 3645 6005 7717 96 Size: 2360 1712 138 Fragments arranged by size: 2360 1712 1443 1149 378 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3106 3138 4525 4557 7719 7751 3106 Size: 32 1387 32 3162 32 3114 Fragments arranged by size: 3162 3114 1387 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3170 1316 Size: 1854 5905 XbaI T'CTAG_A Cuts at: 5095 5095 Size: 7759 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2845 3367 3760 3991 2434 Size: 411 522 393 231 6202 Fragments arranged by size: 6202 522 411 393 231 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7759 XmaI C'CCGG_G Cuts at: 1870 2958 3057 3135 5084 1870 Size: 1088 99 78 1949 4545 Fragments arranged by size: 4545 1949 1088 99 78 XmnI GAAnn'nnTTC Cuts at: 4643 5888 4643 Size: 1245 6514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3574 5567 276 Size: 53 83 186 936 2040 1993 2468 Fragments arranged by size: 2468 2040 1993 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SapI SrfI SwaI