(Circular) (Six-base) MAPSORT of: pFN21AF0165.seq Check: 3365 from: 1 to: 5515 pFN21AF0165 5515 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2661 2858 1937 Size: 724 197 4594 Fragments arranged by size: 4594 724 197 Acc65I G'GTAC_C Cuts at: 2463 2836 2463 Size: 373 5142 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2176 2634 3578 4962 5230 1632 Size: 544 458 944 1384 268 1917 Fragments arranged by size: 1917 1384 944 544 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 4414 5391 1203 Size: 3211 977 1327 Fragments arranged by size: 3211 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2567 1184 Size: 1383 4132 AlwNI CAG_nnn'CTG Cuts at: 2657 3258 4830 2657 Size: 601 1572 3342 Fragments arranged by size: 3342 1572 601 ApaI G_GGCC'C Cuts at: 2035 2035 Size: 5515 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2824 3050 3202 5311 1493 Size: 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2105 2840 1870 Size: 73 162 735 4545 Fragments arranged by size: 4545 735 162 73 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2384 2435 2463 2836 4291 Size: 325 217 627 597 51 28 373 1455 Cuts at: 4291 618 Size: 1842 Fragments arranged by size: 1842 1455 627 597 373 325 217 51 28 BanII G_rGCy'C Cuts at: 1391 2035 2064 2282 2475 2747 2834 1391 Size: 644 29 218 193 272 87 4072 Fragments arranged by size: 4072 644 272 218 193 87 29 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4544 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2678 2712 3706 3740 1903 Size: 34 741 34 994 34 3678 Fragments arranged by size: 3678 994 741 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 1548 Size: 5515 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2171 4126 136 Size: 107 122 71 631 605 499 1955 1525 Fragments arranged by size: 1955 1525 631 605 499 122 107 71 BglII A'GATC_T Cuts at: 2260 2621 5510 2260 Size: 361 2889 2265 Fragments arranged by size: 2889 2265 361 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 2035 2318 2546 2612 3576 4732 1790 Size: 245 283 228 66 964 1156 2573 Fragments arranged by size: 2573 1156 964 283 245 228 66 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2321 3246 4204 Size: 416 53 83 399 24 904 925 958 Cuts at: 4204 442 Size: 1753 Fragments arranged by size: 1753 958 925 904 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5515 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2249 4175 1239 Size: 1010 1926 2579 Fragments arranged by size: 2579 1926 1010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3640 4505 4803 3640 Size: 865 298 4352 Fragments arranged by size: 4352 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2678 2755 4178 882 Size: 617 1179 77 1423 2219 Fragments arranged by size: 2219 1423 1179 617 77 BsaAI yAC'GTr Cuts at: 493 1204 3306 493 Size: 711 2102 2702 Fragments arranged by size: 2702 2102 711 BsaBI GATnn'nnATC Cuts at: 5509 5509 Size: 5515 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3322 3704 4339 275 Size: 53 83 186 936 1789 382 635 1451 Fragments arranged by size: 1789 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2478 2508 1178 Size: 30 165 30 1075 30 4185 Fragments arranged by size: 4185 1075 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2290 1186 Size: 886 218 4411 Fragments arranged by size: 4411 886 218 BseYI C'CCAG_C Cuts at: 4718 5214 4718 Size: 496 5019 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4942 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3576 3661 4732 3576 Size: 85 1071 4359 Fragments arranged by size: 4359 1071 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 BsmBI CGTCTCn'nnnn_ Cuts at: 2448 2448 Size: 5515 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2064 2282 2318 2475 2546 2612 Size: 399 245 29 218 36 157 71 66 Cuts at: 2612 2747 2834 3576 3661 4732 1391 Size: 135 87 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 245 218 157 135 87 85 71 66 36 29 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 2254 3404 5134 2254 Size: 1150 1730 2635 Fragments arranged by size: 2635 1730 1150 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 844 Size: 2026 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 2513 4159 4346 1700 Size: 110 337 366 1646 187 2869 Fragments arranged by size: 2869 1646 366 337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 3575 4587 3575 Size: 1012 4503 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 2775 4319 2775 Size: 1544 3971 BstEII G'GTnAC_C Cuts at: 1184 2350 1184 Size: 1166 4349 BstYI r'GATC_y Cuts at: 1972 2260 2614 2621 2845 3212 3597 3614 5066 Size: 288 354 7 224 367 385 17 1452 Cuts at: 5066 5152 5164 5510 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 354 346 288 224 86 17 12 7 BtgI C'CryG_G Cuts at: 513 1065 2004 2418 513 Size: 552 939 414 3610 Fragments arranged by size: 3610 939 552 414 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4776 Fragments arranged by size: 4776 683 56 BtsI GCAGTG_nn' Cuts at: 3031 3825 3845 3031 Size: 794 20 4701 Fragments arranged by size: 4701 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2348 4522 809 Size: 507 1032 2174 1802 Fragments arranged by size: 2174 1802 1032 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2376 2408 Size: 54 1425 50 275 18 342 204 32 Cuts at: 2408 2962 3851 5480 8 Size: 554 889 1629 43 Fragments arranged by size: 1629 1425 889 554 342 275 204 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3445 1353 Size: 2092 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4102 4476 4622 387 Size: 1064 138 2513 374 146 1280 Fragments arranged by size: 2513 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2249 2634 3578 4175 4962 5230 Size: 393 544 73 385 944 597 787 268 Cuts at: 5230 1239 Size: 1524 Fragments arranged by size: 1524 944 787 597 544 393 385 268 73 EcoNI CCTnn'n_nnAGG Cuts at: 2753 2753 Size: 5515 EcoO109I rG'GnC_Cy Cuts at: 2032 2109 2593 2032 Size: 77 484 4954 Fragments arranged by size: 4954 484 77 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 2680 4662 1678 Size: 1002 1982 2531 Fragments arranged by size: 2531 1982 1002 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2194 2226 2566 2598 2606 Size: 32 135 32 622 32 340 32 8 Cuts at: 2606 2638 2708 2740 4156 4188 4230 4262 1373 Size: 32 70 32 1416 32 42 32 2626 Fragments arranged by size: 2626 1416 622 340 135 70 42 32 32 32 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 669 1657 1938 2859 3105 669 Size: 988 281 921 246 3079 Fragments arranged by size: 3079 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KpnI G_GTAC'C Cuts at: 2467 2840 2467 Size: 373 5142 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2410 10 Size: 54 1425 343 342 236 3115 Fragments arranged by size: 3115 1425 343 342 236 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 3473 3832 3991 518 Size: 648 1191 1116 359 159 2042 Fragments arranged by size: 2042 1191 1116 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2196 2433 2931 3608 4756 5001 1606 Size: 590 237 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 590 498 245 237 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4024 Fragments arranged by size: 4024 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5515 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2873 4418 1132 Size: 97 1644 1545 2229 Fragments arranged by size: 2229 1644 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 4414 4414 Size: 5515 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 1108 Size: 1230 4285 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2109 2593 2109 Size: 484 5031 PshAI GACnn'nnGTC Cuts at: 1613 2690 1613 Size: 1077 4438 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PspOMI G'GGCC_C Cuts at: 2031 2031 Size: 5515 PstI C_TGCA'G Cuts at: 830 2201 2676 2867 830 Size: 1371 475 191 3478 Fragments arranged by size: 3478 1371 475 191 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 PvuII CAG'CTG Cuts at: 2196 2196 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SanDI GG'GwC_CC Cuts at: 2109 2109 Size: 5515 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SexAI A'CCwGG_T Cuts at: 2796 2796 Size: 5515 SfcI C'TryA_G Cuts at: 826 1045 2197 2672 2863 3998 4679 4870 826 Size: 219 1152 475 191 1135 681 191 1471 Fragments arranged by size: 1471 1152 1135 681 475 219 191 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2171 2171 Size: 5515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2107 2842 1872 Size: 235 735 4545 Fragments arranged by size: 4545 735 235 SmlI C'TyrA_G Cuts at: 820 1017 1943 3619 4520 4782 820 Size: 197 926 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3306 493 Size: 2813 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 2043 1517 Size: 526 4989 StyI C'CwwG_G Cuts at: 513 1065 2004 2086 2954 513 Size: 552 939 82 868 3074 Fragments arranged by size: 3074 939 868 552 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2702 2743 3542 3559 3712 3897 4342 Size: 1255 1122 41 799 17 153 185 445 Cuts at: 4342 325 Size: 1498 Fragments arranged by size: 1498 1255 1122 799 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3761 5473 96 Size: 274 80 33 51 1443 1784 1712 138 Fragments arranged by size: 1784 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2478 2510 5475 5507 2121 Size: 32 325 32 2965 32 2129 Fragments arranged by size: 2965 2129 325 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2105 2840 1870 Size: 235 735 4545 Fragments arranged by size: 4545 735 235 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI XcmI