Restriction map for
pFN21AF0165
(Linear) (Six-base) MAPSORT of: FLJ00165.seq Check: 5852 from: 1 to: 810
FLJ00165.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AccI GT'mk_AC
Cuts at: 0 656 810
Size: 656 154
Acc65I G'GTAC_C
Cuts at: 0 458 810
Size: 458 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 629 810
Size: 171 458 181
Fragments arranged by size:
458 181 171
AleI CACnn'nnGTG
Cuts at: 0 352 810
Size: 352 458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 562 810
Size: 562 248
AlwNI CAG_nnn'CTG
Cuts at: 0 652 810
Size: 652 158
ApaI G_GGCC'C
Cuts at: 0 30 810
Size: 30 780
AvaI C'yCGr_G
Cuts at: 0 100 810
Size: 100 710
BanI G'GyrC_C
Cuts at: 0 379 430 458 810
Size: 379 51 28 352
Fragments arranged by size:
379 352 51 28
BanII G_rGCy'C
Cuts at: 0 30 59 277 470 742 810
Size: 30 29 218 193 272 68
Fragments arranged by size:
272 218 193 68 30 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 673 707 810
Size: 673 34 103
Fragments arranged by size:
673 103 34
BglI GCCn_nnn'nGGC
Cuts at: 0 166 810
Size: 166 644
BglII A'GATC_T
Cuts at: 0 255 616 810
Size: 255 361 194
Fragments arranged by size:
361 255 194
Bme1580I G_kGCm'C
Cuts at: 0 30 313 541 607 810
Size: 30 283 228 66 203
Fragments arranged by size:
283 228 203 66 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 316 810
Size: 316 494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 810
Size: 244 566
BsaI GGTCTCn'nnnn_
Cuts at: 0 673 750 810
Size: 673 77 60
Fragments arranged by size:
673 77 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 473 503 810
Size: 473 30 307
Fragments arranged by size:
473 307 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 285 810
Size: 67 218 525
Fragments arranged by size:
525 218 67
BsmBI CGTCTCn'nnnn_
Cuts at: 0 443 810
Size: 443 367
Bsp1286I G_dGCh'C
Cuts at: 0 30 59 277 313 470 541 607 742
Size: 30 29 218 36 157 71 66 135
Cuts at: 742 810
Size: 68
Fragments arranged by size:
218 157 135 71 68 66 36 30
29
BspHI T'CATG_A
Cuts at: 0 249 810
Size: 249 561
BsrFI r'CCGG_y
Cuts at: 0 142 508 810
Size: 142 366 302
Fragments arranged by size:
366 302 142
BstBI TT'CG_AA
Cuts at: 0 770 810
Size: 770 40
BstEII G'GTnAC_C
Cuts at: 0 345 810
Size: 345 465
BstYI r'GATC_y
Cuts at: 0 255 609 616 810
Size: 255 354 7 194
Fragments arranged by size:
354 255 194 7
BtgI C'CryG_G
Cuts at: 0 413 810
Size: 413 397
DrdI GACnn_nn'nnGTC
Cuts at: 0 343 810
Size: 343 467
EaeI y'GGCC_r
Cuts at: 0 167 371 403 810
Size: 167 204 32 407
Fragments arranged by size:
407 204 167 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 244 629 810
Size: 171 73 385 181
Fragments arranged by size:
385 181 171 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 748 810
Size: 748 62
EcoO109I rG'GnC_Cy
Cuts at: 0 27 104 588 810
Size: 27 77 484 222
Fragments arranged by size:
484 222 77 27
HaeII r_GCGC'y
Cuts at: 0 675 810
Size: 675 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 561 593 601 633 703 735
Size: 189 32 340 32 8 32 70 32
Cuts at: 735 810
Size: 75
Fragments arranged by size:
340 189 75 70 32 32 32 32
8
KpnI G_GTAC'C
Cuts at: 0 462 810
Size: 462 348
MscI TGG'CCA
Cuts at: 0 169 405 810
Size: 169 236 405
Fragments arranged by size:
405 236 169
MslI CAynn'nnrTG
Cuts at: 0 352 810
Size: 352 458
MspA1I CmG'CkG
Cuts at: 0 191 428 810
Size: 191 237 382
Fragments arranged by size:
382 237 191
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 810
Size: 333 477
PpuMI rG'GwC_Cy
Cuts at: 0 104 588 810
Size: 104 484 222
Fragments arranged by size:
484 222 104
PshAI GACnn'nnGTC
Cuts at: 0 685 810
Size: 685 125
PspOMI G'GGCC_C
Cuts at: 0 26 810
Size: 26 784
PstI C_TGCA'G
Cuts at: 0 196 671 810
Size: 196 475 139
Fragments arranged by size:
475 196 139
PvuII CAG'CTG
Cuts at: 0 191 810
Size: 191 619
SanDI GG'GwC_CC
Cuts at: 0 104 810
Size: 104 706
SexAI A'CCwGG_T
Cuts at: 0 791 810
Size: 791 19
SfcI C'TryA_G
Cuts at: 0 192 667 810
Size: 192 475 143
Fragments arranged by size:
475 192 143
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 166 810
Size: 166 644
SmaI CCC'GGG
Cuts at: 0 102 810
Size: 102 708
StuI AGG'CCT
Cuts at: 0 38 810
Size: 38 772
StyI C'CwwG_G
Cuts at: 0 81 810
Size: 81 729
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 738 810
Size: 697 41 72
Fragments arranged by size:
697 72 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 473 505 810
Size: 116 32 325 32 305
Fragments arranged by size:
325 305 116 32 32
XmaI C'CCGG_G
Cuts at: 0 100 810
Size: 100 710
Enzymes that do cut:
AccI Acc65I AcuI AleI AlfI AlwNI ApaI AvaI
BanI BanII BcgI BglI BglII Bme1580I BmrI BpmI
BsaI BsaXI BseRI BsmBI Bsp1286I BspHI BsrFI BstBI
BstEII BstYI BtgI DrdI EaeI Eco57MI EcoNI EcoO109I
HaeII Hin4I KpnI MscI MslI MspA1I PflMI PpuMI
PshAI PspOMI PstI PvuII SanDI SexAI SfcI SfiI
SmaI StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI ZraI