(Circular) (Six-base) MAPSORT of: pFN21AF0162.seq Check: 9623 from: 1 to: 6820 pFN21AF0162 6820 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4630 278 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3267 4163 1937 Size: 1330 896 4594 Fragments arranged by size: 4594 1330 896 Acc65I G'GTAC_C Cuts at: 4141 4141 Size: 6820 AclI AA'CG_TT Cuts at: 1563 4947 5320 1563 Size: 3384 373 3063 Fragments arranged by size: 3384 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2025 2579 3863 4883 6267 6535 1632 Size: 393 554 1284 1020 1384 268 1917 Fragments arranged by size: 1917 1384 1284 1020 554 393 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6623 AflIII A'CryG_T Cuts at: 1203 3111 3151 5719 6696 1203 Size: 1908 40 2568 977 1327 Fragments arranged by size: 2568 1908 1327 977 40 AgeI A'CCGG_T Cuts at: 5651 5651 Size: 6820 AhdI GACnn_n'nnGTC Cuts at: 3754 5549 3754 Size: 1795 5025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6820 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2457 1184 Size: 1273 5547 AlwNI CAG_nnn'CTG Cuts at: 4563 6135 4563 Size: 1572 5248 ApaI G_GGCC'C Cuts at: 3405 3405 Size: 6820 ApaLI G'TGCA_C Cuts at: 4877 6033 4877 Size: 1156 5664 ApoI r'AATT_y Cuts at: 1493 4129 4355 4507 6616 1493 Size: 2636 226 152 2109 1697 Fragments arranged by size: 2636 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5374 160 Size: 872 4342 1606 Fragments arranged by size: 4342 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 AvaI C'yCGr_G Cuts at: 1870 1943 4145 1870 Size: 73 2202 4545 Fragments arranged by size: 4545 2202 73 BamHI G'GATC_C Cuts at: 4150 4517 4150 Size: 367 6453 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3105 3971 4141 5596 618 Size: 325 217 627 1318 866 170 1455 1842 Fragments arranged by size: 1842 1455 1318 866 627 325 217 170 BanII G_rGCy'C Cuts at: 1391 2915 3122 3405 3495 3654 3792 4139 1391 Size: 1524 207 283 90 159 138 347 4072 Fragments arranged by size: 4072 1524 347 283 207 159 138 90 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3557 928 Size: 971 1658 4191 Fragments arranged by size: 4191 1658 971 BbvCI CC'TCA_GC Cuts at: 3701 3701 Size: 6820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3995 4029 5011 5045 1903 Size: 34 2058 34 982 34 3678 Fragments arranged by size: 3678 2058 982 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4712 5922 3368 Size: 1344 1210 4266 Fragments arranged by size: 4266 1344 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6820 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3854 5431 136 Size: 107 122 71 631 605 2182 1577 1525 Fragments arranged by size: 2182 1577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3219 3981 6815 3219 Size: 762 2834 3224 Fragments arranged by size: 3224 2834 762 BlpI GC'TnA_GC Cuts at: 2219 4237 2219 Size: 2018 4802 Bme1580I G_kGCm'C Cuts at: 1790 2850 3405 4881 6037 1790 Size: 1060 555 1476 1156 2573 Fragments arranged by size: 2573 1476 1156 1060 555 BmgBI CAC'GTC Cuts at: 1318 2605 3152 4045 1318 Size: 1287 547 893 4093 Fragments arranged by size: 4093 1287 893 547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 3128 3541 Size: 416 53 83 399 24 1137 574 413 Cuts at: 3541 4551 5509 442 Size: 1010 958 1753 Fragments arranged by size: 1753 1137 1010 958 574 416 413 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6820 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2155 2486 2155 Size: 331 6489 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2332 2899 3326 3650 3791 3974 5480 Size: 929 164 567 427 324 141 183 1506 Cuts at: 5480 1239 Size: 2579 Fragments arranged by size: 2579 1506 929 567 427 324 183 164 141 Bpu10I CC'TnA_GC Cuts at: 3701 3701 Size: 6820 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2463 4945 5810 6108 2463 Size: 2482 865 298 3175 Fragments arranged by size: 3175 2482 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 5483 882 Size: 617 525 3459 2219 Fragments arranged by size: 3459 2219 617 525 BsaAI yAC'GTr Cuts at: 493 1204 4611 493 Size: 711 3407 2702 Fragments arranged by size: 3407 2702 711 BsaBI GATnn'nnATC Cuts at: 6814 6814 Size: 6820 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2763 4627 5009 5644 Size: 53 83 186 936 1230 1864 382 635 Cuts at: 5644 275 Size: 1451 Fragments arranged by size: 1864 1451 1230 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2103 3213 5253 5651 5925 6072 6483 2103 Size: 1110 2040 398 274 147 411 2440 Fragments arranged by size: 2440 2040 1110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6595 Fragments arranged by size: 6595 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2165 2223 2350 3059 3689 1186 Size: 856 123 58 127 709 630 4317 Fragments arranged by size: 4317 856 709 630 127 123 58 BseYI C'CCAG_C Cuts at: 2681 3720 6023 6519 2681 Size: 1039 2303 496 2982 Fragments arranged by size: 2982 2303 1039 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2417 1163 Size: 573 681 5566 Fragments arranged by size: 5566 681 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4270 5031 6059 1540 Size: 275 186 2269 761 1028 2301 Fragments arranged by size: 2301 2269 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2661 2679 3495 3792 4881 4966 6037 2661 Size: 18 816 297 1089 85 1071 3444 Fragments arranged by size: 3444 1089 1071 816 297 85 18 BsmI GAATG_Cn' Cuts at: 4331 4424 4331 Size: 93 6727 BsmBI CGTCTCn'nnnn_ Cuts at: 2344 2344 Size: 6820 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2661 2679 2850 2915 3122 3405 3495 Size: 399 871 18 171 65 207 283 90 Cuts at: 3495 3654 3792 4139 4881 4966 6037 1391 Size: 159 138 347 742 85 1071 2174 Fragments arranged by size: 2174 1071 871 742 399 347 283 207 171 159 138 90 85 65 18 BspEI T'CCGG_A Cuts at: 2103 3213 6483 2103 Size: 1110 3270 2440 Fragments arranged by size: 3270 2440 1110 BspHI T'CATG_A Cuts at: 2895 4709 6439 2895 Size: 1814 1730 3276 Fragments arranged by size: 3276 1814 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4175 844 Size: 3331 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3569 4707 1394 Size: 429 1746 1138 3507 Fragments arranged by size: 3507 1746 1138 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4011 5315 5489 65 Size: 1183 2763 1304 174 1396 Fragments arranged by size: 2763 1396 1304 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3794 3829 5464 5651 1700 Size: 110 1984 35 1635 187 2869 Fragments arranged by size: 2869 1984 1635 187 110 35 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6820 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6820 BssSI C'ACGA_G Cuts at: 4880 5892 4880 Size: 1012 5808 BstAPI GCAn_nnn'nTGC Cuts at: 794 2983 3339 794 Size: 2189 356 4275 Fragments arranged by size: 4275 2189 356 BstBI TT'CG_AA Cuts at: 5624 5624 Size: 6820 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6820 BstXI CCAn_nnnn'nTGG Cuts at: 2386 2813 3141 2386 Size: 427 328 6065 Fragments arranged by size: 6065 427 328 BstYI r'GATC_y Cuts at: 1972 2838 3219 3981 4150 4517 4902 4919 6371 Size: 866 381 762 169 367 385 17 1452 Cuts at: 6371 6457 6469 6815 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 866 762 385 381 367 346 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3966 3966 Size: 6820 BtgI C'CryG_G Cuts at: 513 1065 2004 2852 2928 3160 3604 3636 3672 Size: 552 939 848 76 232 444 32 36 Cuts at: 3672 513 Size: 3661 Fragments arranged by size: 3661 939 848 552 444 232 76 36 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6081 Fragments arranged by size: 6081 683 56 BtsI GCAGTG_nn' Cuts at: 2125 2134 2257 4336 5130 5150 2125 Size: 9 123 2079 794 20 3795 Fragments arranged by size: 3795 2079 794 123 20 9 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6820 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6785 DraI TTT'AAA Cuts at: 4124 4124 Size: 6820 DraIII CAC_nnn'GTG Cuts at: 3136 3152 3136 Size: 16 6804 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2528 5827 809 Size: 507 1212 3299 1802 Fragments arranged by size: 3299 1802 1212 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3462 3669 3819 Size: 54 1425 50 275 18 1632 207 150 Cuts at: 3819 3831 4267 5156 6785 8 Size: 12 436 889 1629 43 Fragments arranged by size: 1632 1629 1425 889 436 275 207 150 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 4267 1537 Size: 275 2455 4090 Fragments arranged by size: 4090 2455 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 3251 3448 4026 4750 1353 Size: 699 1199 197 578 724 3423 Fragments arranged by size: 3423 1199 724 699 578 197 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5407 5781 5927 387 Size: 1064 138 3818 374 146 1280 Fragments arranged by size: 3818 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3493 3790 3493 Size: 297 6523 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2025 2168 2332 2579 2899 3326 3650 Size: 393 393 143 164 247 320 427 324 Cuts at: 3650 3791 3863 3974 4883 5480 6267 6535 1239 Size: 141 72 111 909 597 787 268 1524 Fragments arranged by size: 1524 909 787 597 427 393 393 324 320 268 247 164 143 141 111 72 EcoNI CCTnn'n_nnAGG Cuts at: 2347 2435 3074 3929 3953 2347 Size: 88 639 855 24 5214 Fragments arranged by size: 5214 855 639 88 24 EcoO109I rG'GnC_Cy Cuts at: 3401 3402 3401 Size: 1 6819 EcoRI G'AATT_C Cuts at: 4129 4507 4129 Size: 378 6442 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2986 2986 Size: 6820 FspI TGC'GCA Cuts at: 5326 5326 Size: 6820 HaeII r_GCGC'y Cuts at: 1678 5967 1678 Size: 4289 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 3740 3772 4034 Size: 32 135 32 449 32 1687 32 262 Cuts at: 4034 4066 5461 5493 5535 5567 1373 Size: 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1687 1395 449 262 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4164 4410 669 Size: 988 281 2226 246 3079 Fragments arranged by size: 3079 2226 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6820 HpaI GTT'AAC Cuts at: 4410 4410 Size: 6820 KpnI G_GTAC'C Cuts at: 4145 4145 Size: 6820 MfeI C'AATT_G Cuts at: 4419 4419 Size: 6820 MluI A'CGCG_T Cuts at: 6696 6696 Size: 6820 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2388 3969 5934 6118 1305 Size: 1083 1581 1965 184 2007 Fragments arranged by size: 2007 1965 1581 1083 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3671 10 Size: 54 1425 343 1839 3159 Fragments arranged by size: 3159 1839 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2945 3026 3032 3170 3341 3665 4016 Size: 648 1779 81 6 138 171 324 351 Cuts at: 4016 4778 5137 5296 518 Size: 762 359 159 2042 Fragments arranged by size: 2042 1779 762 648 359 351 324 171 159 138 81 6 MspA1I CmG'CkG Cuts at: 1606 2209 2267 2494 2592 3841 4236 4913 6061 Size: 603 58 227 98 1249 395 677 1148 Cuts at: 6061 6306 1606 Size: 245 2120 Fragments arranged by size: 2120 1249 1148 677 603 395 245 227 98 58 NcoI C'CATG_G Cuts at: 513 1065 2004 3604 513 Size: 552 939 1600 3729 Fragments arranged by size: 3729 1600 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6820 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6820 NotI GC'GGCC_GC Cuts at: 4267 4267 Size: 6820 NspI r_CATG'y Cuts at: 1132 1229 2986 3115 4178 5723 1132 Size: 97 1757 129 1063 1545 2229 Fragments arranged by size: 2229 1757 1545 1063 129 97 PasI CC'CwG_GG Cuts at: 1348 2225 1348 Size: 877 5943 PciI A'CATG_T Cuts at: 3111 5719 3111 Size: 2608 4212 PflMI CCAn_nnn'nTGG Cuts at: 1108 2817 3136 3605 3745 1108 Size: 1709 319 469 140 4183 Fragments arranged by size: 4183 1709 469 319 140 PfoI T'CCnGG_A Cuts at: 2348 2915 3366 2348 Size: 567 451 5802 Fragments arranged by size: 5802 567 451 PmeI GTTT'AAAC Cuts at: 4124 4124 Size: 6820 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4889 4921 6402 6434 4889 Size: 32 1481 32 5275 Fragments arranged by size: 5275 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3934 1613 Size: 2321 4499 PsiI TTA'TAA Cuts at: 2112 4390 2112 Size: 2278 4542 PspOMI G'GGCC_C Cuts at: 3401 3401 Size: 6820 PstI C_TGCA'G Cuts at: 830 2336 4172 830 Size: 1506 1836 3478 Fragments arranged by size: 3478 1836 1506 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6820 PvuII CAG'CTG Cuts at: 2267 2494 2592 2267 Size: 227 98 6495 Fragments arranged by size: 6495 227 98 SacI G_AGCT'C Cuts at: 3495 3792 3495 Size: 297 6523 SalI G'TCGA_C Cuts at: 1936 4162 1936 Size: 2226 4594 SapI GCTCTTCn'nnn_ Cuts at: 4026 4026 Size: 6820 SbfI CC_TGCA'GG Cuts at: 4172 4172 Size: 6820 ScaI AGT'ACT Cuts at: 5068 6780 5068 Size: 1712 5108 SfcI C'TryA_G Cuts at: 826 1045 2332 4168 5303 5984 6175 826 Size: 219 1287 1836 1135 681 191 1471 Fragments arranged by size: 1836 1471 1287 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3854 3854 Size: 6820 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6820 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6820 SmaI CCC'GGG Cuts at: 1872 4147 1872 Size: 2275 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2478 4924 5825 6087 820 Size: 197 926 535 2446 901 262 1553 Fragments arranged by size: 2446 1553 926 901 535 262 197 SnaBI TAC'GTA Cuts at: 493 4611 493 Size: 4118 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6820 SphI G_CATG'C Cuts at: 1132 2986 4178 1132 Size: 1854 1192 3774 Fragments arranged by size: 3774 1854 1192 SspI AAT'ATT Cuts at: 5 52 4744 5 Size: 47 4692 2081 Fragments arranged by size: 4692 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6820 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 3604 4259 513 Size: 552 939 237 1363 655 3074 Fragments arranged by size: 3074 1363 939 655 552 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3147 3733 4847 4864 5017 5202 5647 Size: 1255 1567 586 1114 17 153 185 445 Cuts at: 5647 325 Size: 1498 Fragments arranged by size: 1567 1498 1255 1114 586 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2691 5066 6778 Size: 274 80 33 51 1443 714 2375 1712 Cuts at: 6778 96 Size: 138 Fragments arranged by size: 2375 1712 1443 714 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 6780 6812 2844 Size: 32 3904 32 2852 Fragments arranged by size: 3904 2852 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3556 1316 Size: 2240 4580 XbaI T'CTAG_A Cuts at: 4039 4156 4039 Size: 117 6703 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3415 3415 Size: 6820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6820 XmaI C'CCGG_G Cuts at: 1870 4145 1870 Size: 2275 4545 XmnI GAAnn'nnTTC Cuts at: 4949 4949 Size: 6820 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4628 276 Size: 53 83 186 936 3094 2468 Fragments arranged by size: 3094 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI