Restriction map for
pFN21AF0068
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BbrPI BbsI BclI BfrI BlnI
BpuAI BsaAI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
KpnI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SalI SbfI ScaI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AgeI 1950 ACCGGT A^CCGGT
Alw44I 2692 GTGCAC G^TGCAC
AocI 2013 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2912 AGCGCT AGC^GCT
ApaLI 2692 GTGCAC G^TGCAC
Asp700I 2268 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 508 GGATCC G^GATCC
BbeI 2813 GGCGCC GGCGC^C
BcgI 226 GCAGATGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3387 GTATCC GTATCC(6/5)
BlpI 798 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 798 GCTCAGC GC^T[ACGT]AGC
BsaBI 2035 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1950 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3347 TGTACA T^GTACA
BsrBI 2230 GAGCGG CCGCTC(-3/-3)
BsrFI 1950 ACCGGT [GA]^CCGG[CT]
BsrGI 3347 TGTACA T^GTACA
BssSI 2414 CACGAG CACGAG(-5/-1)
BstEII 1230 GGTGACC G^GT[ACGT]ACC
BstPI 1230 GGTGACC G^GT[ACGT]ACC
Bsu36I 2013 CCTGAGG CC^T[ACGT]AGG
CelII 798 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1950 ACCGGT [GA]^CCGG[CT]
DrdI 3187 GACCGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2912 AGCGCT AGC^GCT
Eco81I 2013 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1230 GGTGACC G^GT[ACGT]ACC
EcoRI 1618 GAATTC G^AATTC
HindIII 1924 AAGCTT A^AGCTT
HpaI 2755 GTTAAC GTT^AAC
KasI 2813 GGCGCC G^GCGCC
KspI 2313 CCGCGG CCGC^GG
MamI 2035 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2813 GGCGCC GG^CGCC
NheI 1966 GCTAGC G^CTAGC
NspI 1457 ACATGC [GA]CATG^[CT]
PinAI 1950 ACCGGT A^CCGGT
PvuI 2034 CGATCG CGAT^CG
SacI 2659 GAGCTC GAGCT^C
SacII 2313 CCGCGG CCGC^GG
SfoI 2813 GGCGCC GGC^GCC
SgrAI 1949 CACCGGTG C[GA]^CCGG[CT]G
SspBI 3347 TGTACA T^GTACA
TfiI 2040 GAATC G^A[AT]TC
XbaI 2250 TCTAGA T^CTAGA
XmnI 2268 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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ApaI 2646 GGGCCC GGGCC^C
2881 GGGCCC
AviII 2804 TGCGCA TGC^GCA
3107 TGCGCA
BglII 2978 AGATCT A^GATCT
3470 AGATCT
BsiEI 2034 CGATCG CG[GA][CT]^CG
3186 CGACCG
DraIII 347 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2943 CACTGAGTG
FspI 2804 TGCGCA TGC^GCA
3107 TGCGCA
HaeII 2813 GGCGCC [GA]GCGC^[CT]
2912 AGCGCT
HgaI 2734 GACGC GACGC(5/10)
3235 GCGTC
HphI 352 GGTGA GGTGA(8/7)
1230 GGTGA
MaeII 125 ACGT A^CGT
1699 ACGT
MseI 128 TTAA T^TAA
2756 TTAA
MslI 3009 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3129 CATGGGTGTG
SexAI 836 ACCAGGT A^CC[AT]GGT
1496 ACCAGGT
SmaI 1370 CCCGGG CCC^GGG
1749 CCCGGG
Tru9I 128 TTAA T^TAA
2756 TTAA
Tsp45I 359 GTGAC ^GT[GC]AC
1231 GTGAC
XcmI 1162 CCATGGCTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2588 CCATCCAGCGCATGG
XmaCI 1370 CCCGGG C^CCGGG
1749 CCCGGG
XmaI 1370 CCCGGG C^CCGGG
1749 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TseI Tsp509I TspRI TthHB8I
Van91I XhoII