(Circular) (Six-base) MAPSORT of: pFN21AF0068.seq Check: 6880 from: 1 to: 8278 pFN21AF0068 8278 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3142 3406 4012 5085 3142 Size: 264 606 1073 6335 Fragments arranged by size: 6335 1073 606 264 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6088 278 Size: 53 83 186 936 4552 2468 Fragments arranged by size: 4552 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5621 1937 Size: 3684 4594 Acc65I G'GTAC_C Cuts at: 5599 5599 Size: 8278 AclI AA'CG_TT Cuts at: 1563 6405 6778 1563 Size: 4842 373 3063 Fragments arranged by size: 4842 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 2891 3236 3673 3965 4852 5234 6341 Size: 1013 246 345 437 292 887 382 1107 Cuts at: 6341 7725 7993 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1107 1013 887 437 382 345 292 268 246 AfeI AGC'GCT Cuts at: 4919 4919 Size: 8278 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8081 AflIII A'CryG_T Cuts at: 1203 7177 8154 1203 Size: 5974 977 1327 Fragments arranged by size: 5974 1327 977 AgeI A'CCGG_T Cuts at: 3955 7109 3955 Size: 3154 5124 AhdI GACnn_n'nnGTC Cuts at: 7007 7007 Size: 8278 AleI CACnn'nnGTG Cuts at: 1166 5018 1166 Size: 3852 4426 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2429 4660 1184 Size: 1245 2231 4802 Fragments arranged by size: 4802 2231 1245 AlwNI CAG_nnn'CTG Cuts at: 2740 2848 2920 3496 3550 3658 3775 4124 4633 Size: 108 72 576 54 108 117 349 509 Cuts at: 4633 5526 5545 6021 7593 2740 Size: 893 19 476 1572 3425 Fragments arranged by size: 3425 1572 893 576 509 476 349 117 108 108 72 54 19 ApaI G_GGCC'C Cuts at: 4655 4890 4655 Size: 235 8043 ApaLI G'TGCA_C Cuts at: 4697 6335 7491 4697 Size: 1638 1156 5484 Fragments arranged by size: 5484 1638 1156 ApoI r'AATT_y Cuts at: 1493 2210 2769 3623 5587 5813 5965 8074 1493 Size: 717 559 854 1964 226 152 2109 1697 Fragments arranged by size: 2109 1964 1697 854 717 559 226 152 AseI AT'TA_AT Cuts at: 160 1032 6832 160 Size: 872 5800 1606 Fragments arranged by size: 5800 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8278 AvaI C'yCGr_G Cuts at: 1870 1943 2604 3375 3754 4283 5235 5603 1870 Size: 73 661 771 379 529 952 368 4545 Fragments arranged by size: 4545 952 771 661 529 379 368 73 BamHI G'GATC_C Cuts at: 2513 5608 5975 2513 Size: 3095 367 4816 Fragments arranged by size: 4816 3095 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3648 3795 3897 4133 4410 Size: 325 217 627 1861 147 102 236 277 Cuts at: 4410 4818 5599 7054 618 Size: 408 781 1455 1842 Fragments arranged by size: 1861 1842 1455 781 627 408 325 277 236 217 147 102 BanII G_rGCy'C Cuts at: 1391 2432 2805 3248 3453 3613 3838 3857 4255 Size: 1041 373 443 205 160 225 19 398 Cuts at: 4255 4655 4668 4890 5597 1391 Size: 400 13 222 707 4072 Fragments arranged by size: 4072 1041 707 443 400 398 373 225 222 205 160 19 13 BbeI G_GCGC'C Cuts at: 4822 4822 Size: 8278 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7307 BbvCI CC'TCA_GC Cuts at: 2342 3711 5427 5463 2342 Size: 1369 1716 36 5157 Fragments arranged by size: 5157 1716 1369 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2220 2254 6469 6503 1903 Size: 34 283 34 4215 34 3678 Fragments arranged by size: 4215 3678 283 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5403 6170 7380 5403 Size: 767 1210 6301 Fragments arranged by size: 6301 1210 767 BclI T'GATC_A Cuts at: 1548 1548 Size: 8278 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2831 2887 3195 Size: 107 122 71 631 605 1159 56 308 Cuts at: 3195 6889 136 Size: 3694 1525 Fragments arranged by size: 3694 1525 1159 631 605 308 122 107 71 56 BglII A'GATC_T Cuts at: 4983 5475 8273 4983 Size: 492 2798 4988 Fragments arranged by size: 4988 2798 492 BlpI GC'TnA_GC Cuts at: 2804 5695 2804 Size: 2891 5387 Bme1580I G_kGCm'C Cuts at: 1790 2261 2644 3052 3090 3592 4138 4308 4333 Size: 471 383 408 38 502 546 170 25 Cuts at: 4333 4524 4655 4701 4890 6339 7495 1790 Size: 191 131 46 189 1449 1156 2573 Fragments arranged by size: 2573 1449 1156 546 502 471 408 383 191 189 170 131 46 38 25 BmgBI CAC'GTC Cuts at: 1318 3705 1318 Size: 2387 5891 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2502 2750 4562 Size: 416 53 83 399 24 1085 248 1812 Cuts at: 4562 4824 6009 6967 442 Size: 262 1185 958 1753 Fragments arranged by size: 1812 1753 1185 1085 958 416 399 262 248 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3975 1056 Size: 2919 5359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2039 2086 2325 2516 2918 3041 3389 3455 Size: 800 47 239 191 402 123 348 66 Cuts at: 3455 3761 4146 4370 4415 5017 5325 5441 5491 Size: 306 385 224 45 602 308 116 50 Cuts at: 5491 5579 6938 1239 Size: 88 1359 2579 Fragments arranged by size: 2579 1359 800 602 402 385 348 308 306 239 224 191 123 116 88 66 50 47 45 Bpu10I CC'TnA_GC Cuts at: 2342 2526 3242 3711 5427 5463 2342 Size: 184 716 469 1716 36 5157 Fragments arranged by size: 5157 1716 716 469 184 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2446 3953 4751 6403 7268 7566 2446 Size: 1507 798 1652 865 298 3158 Fragments arranged by size: 3158 1652 1507 865 798 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2460 4949 5301 5352 5381 6941 882 Size: 617 961 2489 352 51 29 1560 2219 Fragments arranged by size: 2489 2219 1560 961 617 352 51 29 BsaAI yAC'GTr Cuts at: 493 1204 6069 493 Size: 711 4865 2702 Fragments arranged by size: 4865 2702 711 BsaBI GATnn'nnATC Cuts at: 4044 8272 4044 Size: 4228 4050 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4740 4819 5240 6085 Size: 53 83 186 936 3207 79 421 845 Cuts at: 6085 6467 7102 275 Size: 382 635 1451 Fragments arranged by size: 3207 1451 936 845 635 421 382 186 83 79 53 BsaWI w'CCGG_w Cuts at: 3955 6711 7109 7383 7530 7941 3955 Size: 2756 398 274 147 411 4292 Fragments arranged by size: 4292 2756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4147 4177 4226 4256 4559 Size: 30 165 30 2744 30 49 30 303 Cuts at: 4559 4589 4904 4934 1178 Size: 30 315 30 4522 Fragments arranged by size: 4522 2744 315 303 165 49 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 2195 2263 2979 3452 3728 4143 4868 Size: 934 75 68 716 473 276 415 725 Cuts at: 4868 1186 Size: 4596 Fragments arranged by size: 4596 934 725 716 473 415 276 75 68 BseYI C'CCAG_C Cuts at: 2224 2650 3090 3443 3558 3612 3642 3850 3882 Size: 426 440 353 115 54 30 208 32 Cuts at: 3882 5171 5362 5396 5437 7481 7977 2224 Size: 1289 191 34 41 2044 496 2525 Fragments arranged by size: 2525 2044 1289 496 440 426 353 208 191 115 54 41 34 32 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2101 2692 3293 3374 3767 3984 4472 Size: 573 365 591 601 81 393 217 488 Cuts at: 4472 5330 1163 Size: 858 4111 Fragments arranged by size: 4111 858 601 591 573 488 393 365 217 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4042 5194 5728 6489 7517 1540 Size: 275 186 2041 1152 534 761 1028 2301 Fragments arranged by size: 2301 2041 1152 1028 761 534 275 186 BsiHKAI G_wGCw'C Cuts at: 2779 4668 4701 6339 6424 7495 2779 Size: 1889 33 1638 85 1071 3562 Fragments arranged by size: 3562 1889 1638 1071 85 33 BsmI GAATG_Cn' Cuts at: 5789 5882 5789 Size: 93 8185 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2432 2644 2779 2805 3052 3090 Size: 399 471 171 212 135 26 247 38 Cuts at: 3090 3248 3453 3592 3613 3838 3857 4138 4255 Size: 158 205 139 21 225 19 281 117 Cuts at: 4255 4308 4333 4524 4655 4668 4701 4890 5597 Size: 53 25 191 131 13 33 189 707 Cuts at: 5597 6339 6424 7495 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 707 471 399 281 247 225 212 205 191 189 171 158 139 135 131 117 85 53 38 33 26 25 21 19 13 BspEI T'CCGG_A Cuts at: 7941 7941 Size: 8278 BspHI T'CATG_A Cuts at: 6167 7897 6167 Size: 1730 6548 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2715 3142 3406 3508 4012 4078 4590 Size: 1278 593 427 264 102 504 66 512 Cuts at: 4590 4734 4980 5085 5352 5633 844 Size: 144 246 105 267 281 3489 Fragments arranged by size: 3489 1278 593 512 504 427 281 267 264 246 144 105 102 66 BsrBI CCG'CTC Cuts at: 1394 1823 4237 6165 1394 Size: 429 2414 1928 3507 Fragments arranged by size: 3507 2414 1928 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3270 5163 5244 6773 6947 65 Size: 1183 2022 1893 81 1529 174 1396 Fragments arranged by size: 2022 1893 1529 1396 1183 174 81 BsrFI r'CCGG_y Cuts at: 1700 1810 3955 6922 7109 1700 Size: 110 2145 2967 187 2869 Fragments arranged by size: 2967 2869 2145 187 110 BsrGI T'GTAC_A Cuts at: 96 5352 96 Size: 5256 3022 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8278 BssSI C'ACGA_G Cuts at: 4419 6338 7350 4419 Size: 1919 1012 5347 Fragments arranged by size: 5347 1919 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3047 3154 3775 4786 794 Size: 2253 107 621 1011 4286 Fragments arranged by size: 4286 2253 1011 621 107 BstBI TT'CG_AA Cuts at: 7082 7082 Size: 8278 BstEII G'GTnAC_C Cuts at: 1184 3235 1184 Size: 2051 6227 BstXI CCAn_nnnn'nTGG Cuts at: 2584 3850 4340 5140 2584 Size: 1266 490 800 5722 Fragments arranged by size: 5722 1266 800 490 BstYI r'GATC_y Cuts at: 1972 2513 4983 5475 5608 5975 6360 6377 7829 Size: 541 2470 492 133 367 385 17 1452 Cuts at: 7829 7915 7927 8273 1972 Size: 86 12 346 1977 Fragments arranged by size: 2470 1977 1452 541 492 385 367 346 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4019 4019 Size: 8278 BtgI C'CryG_G Cuts at: 513 1065 2004 3096 3167 3843 4230 4318 4734 Size: 552 939 1092 71 676 387 88 416 Cuts at: 4734 4882 5133 513 Size: 148 251 3658 Fragments arranged by size: 3658 1092 939 676 552 416 387 251 148 88 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2426 487 Size: 683 56 1200 6339 Fragments arranged by size: 6339 1200 683 56 BtsI GCAGTG_nn' Cuts at: 2631 4078 4369 4616 5794 6588 6608 2631 Size: 1447 291 247 1178 794 20 4301 Fragments arranged by size: 4301 1447 1178 794 291 247 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8278 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4879 4914 5063 5098 529 Size: 35 4315 35 149 35 3709 Fragments arranged by size: 4315 3709 149 35 35 35 DraI TTT'AAA Cuts at: 5582 5582 Size: 8278 DraIII CAC_nnn'GTG Cuts at: 2357 4953 2357 Size: 2596 5682 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5198 7285 809 Size: 507 3882 2087 1802 Fragments arranged by size: 3882 2087 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2574 2586 3425 Size: 54 1425 50 275 18 744 12 839 Cuts at: 3425 3840 4092 4227 4731 5443 5725 6614 8243 Size: 415 252 135 504 712 282 889 1629 Cuts at: 8243 8 Size: 43 Fragments arranged by size: 1629 1425 889 839 744 712 504 415 282 275 252 135 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 5725 1537 Size: 275 3913 4090 Fragments arranged by size: 4090 3913 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 2798 3692 4871 5373 6208 1353 Size: 744 701 894 1179 502 835 3423 Fragments arranged by size: 3423 1179 894 835 744 701 502 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2681 3677 3857 4982 6865 7239 Size: 1064 138 1092 996 180 1125 1883 374 Cuts at: 7239 7385 387 Size: 146 1280 Fragments arranged by size: 1883 1280 1125 1092 1064 996 374 180 146 138 EcoICRI GAG'CTC Cuts at: 4666 4666 Size: 8278 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2086 2325 2516 2645 2891 2918 Size: 393 407 47 239 191 129 246 27 Cuts at: 2918 3041 3236 3389 3455 3673 3761 3965 4146 Size: 123 195 153 66 218 88 204 181 Cuts at: 4146 4370 4415 4852 5017 5234 5325 5441 5491 Size: 224 45 437 165 217 91 116 50 Cuts at: 5491 5579 6341 6938 7725 7993 1239 Size: 88 762 597 787 268 1524 Fragments arranged by size: 1524 787 762 597 437 407 393 268 246 239 224 218 217 204 195 191 181 165 153 129 123 116 91 88 88 66 50 47 45 27 EcoNI CCTnn'n_nnAGG Cuts at: 2203 3056 4022 4142 4167 4850 2203 Size: 853 966 120 25 683 5631 Fragments arranged by size: 5631 966 853 683 120 25 EcoO109I rG'GnC_Cy Cuts at: 2013 2137 2144 2297 2392 3139 3226 3346 3991 Size: 124 7 153 95 747 87 120 645 Cuts at: 3991 4312 4651 4652 5286 5334 5542 5554 2013 Size: 321 339 1 634 48 208 12 4737 Fragments arranged by size: 4737 747 645 634 339 321 208 153 124 120 95 87 48 12 7 1 EcoRI G'AATT_C Cuts at: 3623 5587 5965 3623 Size: 1964 378 5936 Fragments arranged by size: 5936 1964 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4939 4939 Size: 8278 FspI TGC'GCA Cuts at: 4811 5114 6784 4811 Size: 303 1670 6305 Fragments arranged by size: 6305 1670 303 FspAI rTGC'GCAy Cuts at: 4811 5114 4811 Size: 303 7975 HaeII r_GCGC'y Cuts at: 1678 4822 4921 7425 1678 Size: 3144 99 2504 2531 Fragments arranged by size: 3144 2531 2504 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 2505 2537 3820 Size: 32 135 32 887 32 14 32 1283 Cuts at: 3820 3852 4559 4591 5114 5146 5300 5332 5465 Size: 32 707 32 523 32 154 32 133 Cuts at: 5465 5497 6919 6951 6993 7025 1373 Size: 32 1422 32 42 32 2626 Fragments arranged by size: 2626 1422 1283 887 707 523 154 135 133 42 32 32 32 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 4762 4900 5203 5622 5868 669 Size: 988 281 2824 138 303 419 246 3079 Fragments arranged by size: 3079 2824 988 419 303 281 246 138 HindIII A'AGCT_T Cuts at: 748 3929 748 Size: 3181 5097 HpaI GTT'AAC Cuts at: 4762 5868 4762 Size: 1106 7172 KasI G'GCGC_C Cuts at: 4818 4818 Size: 8278 KpnI G_GTAC'C Cuts at: 5603 5603 Size: 8278 MfeI C'AATT_G Cuts at: 5877 5877 Size: 8278 MluI A'CGCG_T Cuts at: 8154 8154 Size: 8278 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7392 7576 1305 Size: 6087 184 2007 Fragments arranged by size: 6087 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2576 2588 3427 3842 4229 Size: 54 1425 343 744 12 839 415 387 Cuts at: 4229 4733 10 Size: 504 3555 Fragments arranged by size: 3555 1425 839 744 504 415 387 343 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 5018 5138 6236 6595 6754 518 Size: 648 3852 120 1098 359 159 2042 Fragments arranged by size: 3852 2042 1098 648 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2228 2896 3121 3550 3616 3646 3655 3775 Size: 622 668 225 429 66 30 9 120 Cuts at: 3775 3781 3882 3998 4207 4320 4708 4783 5063 Size: 6 101 116 209 113 388 75 280 Cuts at: 5063 5382 5526 5694 6371 7519 7764 1606 Size: 319 144 168 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 668 622 429 388 319 280 245 225 209 168 144 120 116 113 101 75 66 30 9 6 NarI GG'CG_CC Cuts at: 4819 4819 Size: 8278 NcoI C'CATG_G Cuts at: 513 1065 2004 3096 3167 4734 4882 5133 513 Size: 552 939 1092 71 1567 148 251 3658 Fragments arranged by size: 3658 1567 1092 939 552 251 148 71 NdeI CA'TA_TG Cuts at: 387 387 Size: 8278 NheI G'CTAG_C Cuts at: 1052 3971 1052 Size: 2919 5359 NotI GC'GGCC_GC Cuts at: 5725 5725 Size: 8278 NspI r_CATG'y Cuts at: 1132 1229 3466 5636 7181 1132 Size: 97 2237 2170 1545 2229 Fragments arranged by size: 2237 2229 2170 1545 97 PasI CC'CwG_GG Cuts at: 1348 2372 2432 2828 3584 4309 5545 1348 Size: 1024 60 396 756 725 1236 4081 Fragments arranged by size: 4081 1236 1024 756 725 396 60 PciI A'CATG_T Cuts at: 7177 7177 Size: 8278 PflMI CCAn_nnn'nTGG Cuts at: 1108 2064 3097 3367 3658 4124 4603 1108 Size: 956 1033 270 291 466 479 4783 Fragments arranged by size: 4783 1033 956 479 466 291 270 PfoI T'CCnGG_A Cuts at: 2204 5302 2204 Size: 3098 5180 PmeI GTTT'AAAC Cuts at: 5582 5582 Size: 8278 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4973 5005 6347 6379 7860 7892 4973 Size: 32 1342 32 1481 32 5359 Fragments arranged by size: 5359 1481 1342 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2137 2144 2392 3139 3226 3991 5334 2137 Size: 7 248 747 87 765 1343 5081 Fragments arranged by size: 5081 1343 765 747 248 87 7 PshAI GACnn'nnGTC Cuts at: 1613 2142 2397 3137 4941 1613 Size: 529 255 740 1804 4950 Fragments arranged by size: 4950 1804 740 529 255 PsiI TTA'TAA Cuts at: 5848 5848 Size: 8278 PspOMI G'GGCC_C Cuts at: 4651 4886 4651 Size: 235 8043 PstI C_TGCA'G Cuts at: 830 2233 2895 3327 3414 3546 3780 4629 5028 Size: 1403 662 432 87 132 234 849 399 Cuts at: 5028 5630 830 Size: 602 3478 Fragments arranged by size: 3478 1403 849 662 602 432 399 234 132 87 PvuI CG_AT'CG Cuts at: 2001 4042 2001 Size: 2041 6237 PvuII CAG'CTG Cuts at: 2228 2896 3121 3616 3775 3781 4207 4708 4783 Size: 668 225 495 159 6 426 501 75 Cuts at: 4783 5063 5526 2228 Size: 280 463 4980 Fragments arranged by size: 4980 668 501 495 463 426 280 225 159 75 6 SacI G_AGCT'C Cuts at: 4668 4668 Size: 8278 SacII CC_GC'GG Cuts at: 4321 4321 Size: 8278 SalI G'TCGA_C Cuts at: 1936 5620 1936 Size: 3684 4594 SanDI GG'GwC_CC Cuts at: 2392 5334 2392 Size: 2942 5336 SapI GCTCTTCn'nnn_ Cuts at: 3692 4871 5373 3692 Size: 1179 502 6597 Fragments arranged by size: 6597 1179 502 SbfI CC_TGCA'GG Cuts at: 5630 5630 Size: 8278 ScaI AGT'ACT Cuts at: 6526 8238 6526 Size: 1712 6566 SexAI A'CCwGG_T Cuts at: 2841 3501 2841 Size: 660 7618 SfcI C'TryA_G Cuts at: 826 1045 2186 2229 2891 3026 3125 3253 3323 Size: 219 1141 43 662 135 99 128 70 Cuts at: 3323 3410 3542 3776 4030 4202 4474 4625 5024 Size: 87 132 234 254 172 272 151 399 Cuts at: 5024 5626 6761 7442 7633 826 Size: 602 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 681 662 602 399 272 254 234 219 191 172 151 135 132 128 99 87 70 43 SfoI GGC'GCC Cuts at: 4820 4820 Size: 8278 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8278 SgrAI Cr'CCGG_yG Cuts at: 1700 3955 1700 Size: 2255 6023 SmaI CCC'GGG Cuts at: 1872 3377 3756 5605 1872 Size: 1505 379 1849 4545 Fragments arranged by size: 4545 1849 1505 379 SmlI C'TyrA_G Cuts at: 820 1017 1943 2425 3932 4766 6382 7283 7545 Size: 197 926 482 1507 834 1616 901 262 Cuts at: 7545 820 Size: 1553 Fragments arranged by size: 1616 1553 1507 926 901 834 482 262 197 SnaBI TAC'GTA Cuts at: 493 6069 493 Size: 5576 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8278 SphI G_CATG'C Cuts at: 1132 5636 1132 Size: 4504 3774 SspI AAT'ATT Cuts at: 5 52 6202 5 Size: 47 6150 2081 Fragments arranged by size: 6150 2081 47 StuI AGG'CCT Cuts at: 1517 2970 2990 3698 4028 5030 5054 5153 1517 Size: 1453 20 708 330 1002 24 99 4642 Fragments arranged by size: 4642 1453 1002 708 330 99 24 20 StyI C'CwwG_G Cuts at: 513 1065 2004 2461 2614 3096 3167 3478 4734 Size: 552 939 457 153 482 71 311 1256 Cuts at: 4734 4882 4939 5133 5717 513 Size: 148 57 194 584 3074 Fragments arranged by size: 3074 1256 939 584 552 482 457 311 194 153 148 71 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 3104 4428 5126 6305 6322 6475 Size: 1255 606 918 1324 698 1179 17 153 Cuts at: 6475 6660 7105 325 Size: 185 445 1498 Fragments arranged by size: 1498 1324 1255 1179 918 698 606 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2731 4260 5352 Size: 274 80 33 51 1443 754 1529 1092 Cuts at: 5352 6524 8236 96 Size: 1172 1712 138 Fragments arranged by size: 1712 1529 1443 1172 1092 754 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2736 2768 8238 8270 2736 Size: 32 5470 32 2744 Fragments arranged by size: 5470 2744 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8278 XbaI T'CTAG_A Cuts at: 4255 5614 4255 Size: 1359 6919 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3174 4600 3174 Size: 1426 6852 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8278 XmaI C'CCGG_G Cuts at: 1870 3375 3754 5603 1870 Size: 1505 379 1849 4545 Fragments arranged by size: 4545 1849 1505 379 XmnI GAAnn'nnTTC Cuts at: 4277 6407 4277 Size: 2130 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6086 276 Size: 53 83 186 936 4552 2468 Fragments arranged by size: 4552 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SfiI SrfI SwaI