Restriction map for
pFN21AF0068
(Linear) (Six-base) MAPSORT of: FLJ00068.seq Check: 3473 from: 1 to: 3573
FLJ00068.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1137 1401 2007 3080 3573
Size: 1137 264 606 1073 493
Fragments arranged by size:
1137 1073 606 493 264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 886 1231 1668 1960 2847 3229 3573
Size: 640 246 345 437 292 887 382 344
Fragments arranged by size:
887 640 437 382 345 344 292 246
AfeI AGC'GCT
Cuts at: 0 2914 3573
Size: 2914 659
AgeI A'CCGG_T
Cuts at: 0 1950 3573
Size: 1950 1623
AleI CACnn'nnGTG
Cuts at: 0 3013 3573
Size: 3013 560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 2655 3573
Size: 424 2231 918
Fragments arranged by size:
2231 918 424
AlwNI CAG_nnn'CTG
Cuts at: 0 735 843 915 1491 1545 1653 1770 2119
Size: 735 108 72 576 54 108 117 349
Cuts at: 2119 2628 3521 3540 3573
Size: 509 893 19 33
Fragments arranged by size:
893 735 576 509 349 117 108 108
72 54 33 19
ApaI G_GGCC'C
Cuts at: 0 2650 2885 3573
Size: 2650 235 688
Fragments arranged by size:
2650 688 235
ApaLI G'TGCA_C
Cuts at: 0 2692 3573
Size: 2692 881
ApoI r'AATT_y
Cuts at: 0 205 764 1618 3573
Size: 205 559 854 1955
Fragments arranged by size:
1955 854 559 205
AvaI C'yCGr_G
Cuts at: 0 599 1370 1749 2278 3230 3573
Size: 599 771 379 529 952 343
Fragments arranged by size:
952 771 599 529 379 343
BamHI G'GATC_C
Cuts at: 0 508 3573
Size: 508 3065
BanI G'GyrC_C
Cuts at: 0 1643 1790 1892 2128 2405 2813 3573
Size: 1643 147 102 236 277 408 760
Fragments arranged by size:
1643 760 408 277 236 147 102
BanII G_rGCy'C
Cuts at: 0 427 800 1243 1448 1608 1833 1852 2250
Size: 427 373 443 205 160 225 19 398
Cuts at: 2250 2650 2663 2885 3573
Size: 400 13 222 688
Fragments arranged by size:
688 443 427 400 398 373 225 222
205 160 19 13
BbeI G_GCGC'C
Cuts at: 0 2817 3573
Size: 2817 756
BbvCI CC'TCA_GC
Cuts at: 0 337 1706 3422 3458 3573
Size: 337 1369 1716 36 115
Fragments arranged by size:
1716 1369 337 115 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 215 249 3573
Size: 215 34 3324
Fragments arranged by size:
3324 215 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3398 3573
Size: 3398 175
BglI GCCn_nnn'nGGC
Cuts at: 0 826 882 1190 3573
Size: 826 56 308 2383
Fragments arranged by size:
2383 826 308 56
BglII A'GATC_T
Cuts at: 0 2978 3470 3573
Size: 2978 492 103
Fragments arranged by size:
2978 492 103
BlpI GC'TnA_GC
Cuts at: 0 799 3573
Size: 799 2774
Bme1580I G_kGCm'C
Cuts at: 0 256 639 1047 1085 1587 2133 2303 2328
Size: 256 383 408 38 502 546 170 25
Cuts at: 2328 2519 2650 2696 2885 3573
Size: 191 131 46 189 688
Fragments arranged by size:
688 546 502 408 383 256 191 189
170 131 46 38 25
BmgBI CAC'GTC
Cuts at: 0 1700 3573
Size: 1700 1873
BmrI ACTGGGnnnn_n'
Cuts at: 0 497 745 2557 2819 3573
Size: 497 248 1812 262 754
Fragments arranged by size:
1812 754 497 262 248
BmtI G_CTAG'C
Cuts at: 0 1970 3573
Size: 1970 1603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 81 320 511 913 1036 1384 1450
Size: 34 47 239 191 402 123 348 66
Cuts at: 1450 1756 2141 2365 2410 3012 3320 3436 3486
Size: 306 385 224 45 602 308 116 50
Cuts at: 3486 3573
Size: 87
Fragments arranged by size:
602 402 385 348 308 306 239 224
191 123 116 87 66 50 47 45
34
Bpu10I CC'TnA_GC
Cuts at: 0 337 521 1237 1706 3422 3458 3573
Size: 337 184 716 469 1716 36 115
Fragments arranged by size:
1716 716 469 337 184 115 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 1948 2746 3573
Size: 441 1507 798 827
Fragments arranged by size:
1507 827 798 441
BsaI GGTCTCn'nnnn_
Cuts at: 0 455 2944 3296 3347 3376 3573
Size: 455 2489 352 51 29 197
Fragments arranged by size:
2489 455 352 197 51 29
BsaBI GATnn'nnATC
Cuts at: 0 2039 3573
Size: 2039 1534
BsaHI Gr'CG_yC
Cuts at: 0 2735 2814 3235 3573
Size: 2735 79 421 338
Fragments arranged by size:
2735 421 338 79
BsaWI w'CCGG_w
Cuts at: 0 1950 3573
Size: 1950 1623
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2142 2172 2221 2251 2554 2584 2899 2929
Size: 2142 30 49 30 303 30 315 30
Cuts at: 2929 3573
Size: 644
Fragments arranged by size:
2142 644 315 303 49 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 190 258 974 1447 1723 2138 2863
Size: 115 75 68 716 473 276 415 725
Cuts at: 2863 3573
Size: 710
Fragments arranged by size:
725 716 710 473 415 276 115 75
68
BseYI C'CCAG_C
Cuts at: 0 219 645 1085 1438 1553 1607 1637 1845
Size: 219 426 440 353 115 54 30 208
Cuts at: 1845 1877 3166 3357 3391 3432 3573
Size: 32 1289 191 34 41 141
Fragments arranged by size:
1289 440 426 353 219 208 191 141
115 54 41 34 32 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 687 1288 1369 1762 1979 2467 3325
Size: 96 591 601 81 393 217 488 858
Cuts at: 3325 3573
Size: 248
Fragments arranged by size:
858 601 591 488 393 248 217 96
81
BsiEI CG_ry'CG
Cuts at: 0 2037 3189 3573
Size: 2037 1152 384
Fragments arranged by size:
2037 1152 384
BsiHKAI G_wGCw'C
Cuts at: 0 774 2663 2696 3573
Size: 774 1889 33 877
Fragments arranged by size:
1889 877 774 33
Bsp1286I G_dGCh'C
Cuts at: 0 256 427 639 774 800 1047 1085 1243
Size: 256 171 212 135 26 247 38 158
Cuts at: 1243 1448 1587 1608 1833 1852 2133 2250 2303
Size: 205 139 21 225 19 281 117 53
Cuts at: 2303 2328 2519 2650 2663 2696 2885 3573
Size: 25 191 131 13 33 189 688
Fragments arranged by size:
688 281 256 247 225 212 205 191
189 171 158 139 135 131 117 53
38 33 26 25 21 19 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 710 1137 1401 1503 2007 2073 2585
Size: 117 593 427 264 102 504 66 512
Cuts at: 2585 2729 2975 3080 3347 3573
Size: 144 246 105 267 226
Fragments arranged by size:
593 512 504 427 267 264 246 226
144 117 105 102 66
BsrBI CCG'CTC
Cuts at: 0 2232 3573
Size: 2232 1341
BsrDI GCAATG_nn'
Cuts at: 0 1265 3158 3239 3573
Size: 1265 1893 81 334
Fragments arranged by size:
1893 1265 334 81
BsrFI r'CCGG_y
Cuts at: 0 1950 3573
Size: 1950 1623
BsrGI T'GTAC_A
Cuts at: 0 3347 3573
Size: 3347 226
BssSI C'ACGA_G
Cuts at: 0 2414 3573
Size: 2414 1159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1042 1149 1770 2781 3573
Size: 1042 107 621 1011 792
Fragments arranged by size:
1042 1011 792 621 107
BstEII G'GTnAC_C
Cuts at: 0 1230 3573
Size: 1230 2343
BstXI CCAn_nnnn'nTGG
Cuts at: 0 579 1845 2335 3135 3573
Size: 579 1266 490 800 438
Fragments arranged by size:
1266 800 579 490 438
BstYI r'GATC_y
Cuts at: 0 508 2978 3470 3573
Size: 508 2470 492 103
Fragments arranged by size:
2470 508 492 103
Bsu36I CC'TnA_GG
Cuts at: 0 2014 3573
Size: 2014 1559
BtgI C'CryG_G
Cuts at: 0 1091 1162 1838 2225 2313 2729 2877 3128
Size: 1091 71 676 387 88 416 148 251
Cuts at: 3128 3573
Size: 445
Fragments arranged by size:
1091 676 445 416 387 251 148 88
71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 421 3573
Size: 421 3152
BtsI GCAGTG_nn'
Cuts at: 0 626 2073 2364 2611 3573
Size: 626 1447 291 247 962
Fragments arranged by size:
1447 962 626 291 247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2874 2909 3058 3093 3573
Size: 2874 35 149 35 480
Fragments arranged by size:
2874 480 149 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 352 2948 3573
Size: 352 2596 625
Fragments arranged by size:
2596 625 352
DrdI GACnn_nn'nnGTC
Cuts at: 0 3193 3573
Size: 3193 380
EaeI y'GGCC_r
Cuts at: 0 569 581 1420 1835 2087 2222 2726 3438
Size: 569 12 839 415 252 135 504 712
Cuts at: 3438 3573
Size: 135
Fragments arranged by size:
839 712 569 504 415 252 135 135
12
EarI CTCTTCn'nnn_
Cuts at: 0 92 793 1687 2866 3368 3573
Size: 92 701 894 1179 502 205
Fragments arranged by size:
1179 894 701 502 205 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 1672 1852 2977 3573
Size: 676 996 180 1125 596
Fragments arranged by size:
1125 996 676 596 180
EcoICRI GAG'CTC
Cuts at: 0 2661 3573
Size: 2661 912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 81 320 511 640 886 913 1036
Size: 34 47 239 191 129 246 27 123
Cuts at: 1036 1231 1384 1450 1668 1756 1960 2141 2365
Size: 195 153 66 218 88 204 181 224
Cuts at: 2365 2410 2847 3012 3229 3320 3436 3486 3573
Size: 45 437 165 217 91 116 50 87
Fragments arranged by size:
437 246 239 224 218 217 204 195
191 181 165 153 129 123 116 91
88 87 66 50 47 45 34 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 198 1051 2017 2137 2162 2845 3573
Size: 198 853 966 120 25 683 728
Fragments arranged by size:
966 853 728 683 198 120 25
EcoO109I rG'GnC_Cy
Cuts at: 0 8 132 139 292 387 1134 1221 1341
Size: 8 124 7 153 95 747 87 120
Cuts at: 1341 1986 2307 2646 2647 3281 3329 3537 3549
Size: 645 321 339 1 634 48 208 12
Cuts at: 3549 3573
Size: 24
Fragments arranged by size:
747 645 634 339 321 208 153 124
120 95 87 48 24 12 8 7
1
EcoRI G'AATT_C
Cuts at: 0 1618 3573
Size: 1618 1955
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2934 3573
Size: 2934 639
FspI TGC'GCA
Cuts at: 0 2806 3109 3573
Size: 2806 303 464
Fragments arranged by size:
2806 464 303
FspAI rTGC'GCAy
Cuts at: 0 2806 3109 3573
Size: 2806 303 464
Fragments arranged by size:
2806 464 303
HaeII r_GCGC'y
Cuts at: 0 2817 2916 3573
Size: 2817 99 657
Fragments arranged by size:
2817 657 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 500 532 1815 1847 2554 2586
Size: 454 32 14 32 1283 32 707 32
Cuts at: 2586 3109 3141 3295 3327 3460 3492 3573
Size: 523 32 154 32 133 32 81
Fragments arranged by size:
1283 707 523 454 154 133 81 32
32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 0 2757 2895 3198 3573
Size: 2757 138 303 375
Fragments arranged by size:
2757 375 303 138
HindIII A'AGCT_T
Cuts at: 0 1924 3573
Size: 1924 1649
HpaI GTT'AAC
Cuts at: 0 2757 3573
Size: 2757 816
KasI G'GCGC_C
Cuts at: 0 2813 3573
Size: 2813 760
MscI TGG'CCA
Cuts at: 0 571 583 1422 1837 2224 2728 3573
Size: 571 12 839 415 387 504 845
Fragments arranged by size:
845 839 571 504 415 387 12
MslI CAynn'nnrTG
Cuts at: 0 3013 3133 3573
Size: 3013 120 440
Fragments arranged by size:
3013 440 120
MspA1I CmG'CkG
Cuts at: 0 223 891 1116 1545 1611 1641 1650 1770
Size: 223 668 225 429 66 30 9 120
Cuts at: 1770 1776 1877 1993 2202 2315 2703 2778 3058
Size: 6 101 116 209 113 388 75 280
Cuts at: 3058 3377 3521 3573
Size: 319 144 52
Fragments arranged by size:
668 429 388 319 280 225 223 209
144 120 116 113 101 75 66 52
30 9 6
NarI GG'CG_CC
Cuts at: 0 2814 3573
Size: 2814 759
NcoI C'CATG_G
Cuts at: 0 1091 1162 2729 2877 3128 3573
Size: 1091 71 1567 148 251 445
Fragments arranged by size:
1567 1091 445 251 148 71
NheI G'CTAG_C
Cuts at: 0 1966 3573
Size: 1966 1607
NspI r_CATG'y
Cuts at: 0 1461 3573
Size: 1461 2112
PasI CC'CwG_GG
Cuts at: 0 367 427 823 1579 2304 3540 3573
Size: 367 60 396 756 725 1236 33
Fragments arranged by size:
1236 756 725 396 367 60 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 1092 1362 1653 2119 2598 3573
Size: 59 1033 270 291 466 479 975
Fragments arranged by size:
1033 975 479 466 291 270 59
PfoI T'CCnGG_A
Cuts at: 0 199 3297 3573
Size: 199 3098 276
Fragments arranged by size:
3098 276 199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2968 3000 3573
Size: 2968 32 573
Fragments arranged by size:
2968 573 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 139 387 1134 1221 1986 3329 3573
Size: 132 7 248 747 87 765 1343 244
Fragments arranged by size:
1343 765 747 248 244 132 87 7
PshAI GACnn'nnGTC
Cuts at: 0 137 392 1132 2936 3573
Size: 137 255 740 1804 637
Fragments arranged by size:
1804 740 637 255 137
PspOMI G'GGCC_C
Cuts at: 0 2646 2881 3573
Size: 2646 235 692
Fragments arranged by size:
2646 692 235
PstI C_TGCA'G
Cuts at: 0 228 890 1322 1409 1541 1775 2624 3023
Size: 228 662 432 87 132 234 849 399
Cuts at: 3023 3573
Size: 550
Fragments arranged by size:
849 662 550 432 399 234 228 132
87
PvuI CG_AT'CG
Cuts at: 0 2037 3573
Size: 2037 1536
PvuII CAG'CTG
Cuts at: 0 223 891 1116 1611 1770 1776 2202 2703
Size: 223 668 225 495 159 6 426 501
Cuts at: 2703 2778 3058 3521 3573
Size: 75 280 463 52
Fragments arranged by size:
668 501 495 463 426 280 225 223
159 75 52 6
SacI G_AGCT'C
Cuts at: 0 2663 3573
Size: 2663 910
SacII CC_GC'GG
Cuts at: 0 2316 3573
Size: 2316 1257
SanDI GG'GwC_CC
Cuts at: 0 387 3329 3573
Size: 387 2942 244
Fragments arranged by size:
2942 387 244
SapI GCTCTTCn'nnn_
Cuts at: 0 1687 2866 3368 3573
Size: 1687 1179 502 205
Fragments arranged by size:
1687 1179 502 205
SexAI A'CCwGG_T
Cuts at: 0 836 1496 3573
Size: 836 660 2077
Fragments arranged by size:
2077 836 660
SfcI C'TryA_G
Cuts at: 0 181 224 886 1021 1120 1248 1318 1405
Size: 181 43 662 135 99 128 70 87
Cuts at: 1405 1537 1771 2025 2197 2469 2620 3019 3573
Size: 132 234 254 172 272 151 399 554
Fragments arranged by size:
662 554 399 272 254 234 181 172
151 135 132 128 99 87 70 43
SfoI GGC'GCC
Cuts at: 0 2815 3573
Size: 2815 758
SgrAI Cr'CCGG_yG
Cuts at: 0 1950 3573
Size: 1950 1623
SmaI CCC'GGG
Cuts at: 0 1372 1751 3573
Size: 1372 379 1822
Fragments arranged by size:
1822 1372 379
SmlI C'TyrA_G
Cuts at: 0 420 1927 2761 3573
Size: 420 1507 834 812
Fragments arranged by size:
1507 834 812 420
StuI AGG'CCT
Cuts at: 0 965 985 1693 2023 3025 3049 3148 3573
Size: 965 20 708 330 1002 24 99 425
Fragments arranged by size:
1002 965 708 425 330 99 24 20
StyI C'CwwG_G
Cuts at: 0 456 609 1091 1162 1473 2729 2877 2934
Size: 456 153 482 71 311 1256 148 57
Cuts at: 2934 3128 3573
Size: 194 445
Fragments arranged by size:
1256 482 456 445 311 194 153 148
71 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 1099 2423 3121 3573
Size: 181 918 1324 698 452
Fragments arranged by size:
1324 918 698 452 181
TatI w'GTAC_w
Cuts at: 0 726 2255 3347 3573
Size: 726 1529 1092 226
Fragments arranged by size:
1529 1092 726 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 731 763 3573
Size: 731 32 2810
Fragments arranged by size:
2810 731 32
XbaI T'CTAG_A
Cuts at: 0 2250 3573
Size: 2250 1323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1169 2595 3573
Size: 1169 1426 978
Fragments arranged by size:
1426 1169 978
XmaI C'CCGG_G
Cuts at: 0 1370 1749 3573
Size: 1370 379 1824
Fragments arranged by size:
1824 1370 379
XmnI GAAnn'nnTTC
Cuts at: 0 2272 3573
Size: 2272 1301
Enzymes that do cut:
AarI AcuI AfeI AgeI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI MscI MslI MspA1I NarI NcoI NheI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII SacI SacII SanDI SapI SexAI SfcI
SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI
TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AhdI AloI
AscI AseI AsiSI AvrII BaeI BbsI BclI BfrBI
BplI BsaAI BsiWI BsmI BsmBI BspEI BspHI BssHII
BstBI BstZ17I ClaI DraI EagI EcoRV FseI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PsiI PspXI PsrI
RsrII SalI SbfI ScaI SfiI SgfI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XhoI ZraI