(Circular) (Six-base) MAPSORT of: pFN21AF0052.seq Check: 2986 from: 1 to: 5689 pFN21AF0052 5689 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 2808 3010 2808 Size: 202 5487 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2933 3752 5136 5404 1632 Size: 1301 819 1384 268 1917 Fragments arranged by size: 1917 1384 1301 819 268 AflII C'TTAA_G Cuts at: 820 1017 2681 820 Size: 197 1664 3828 Fragments arranged by size: 3828 1664 197 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2745 1184 Size: 1561 4128 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2337 2369 2337 Size: 32 5657 AlwNI CAG_nnn'CTG Cuts at: 2857 2987 3432 5004 2857 Size: 130 445 1572 3542 Fragments arranged by size: 3542 1572 445 130 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2417 2998 3224 3376 5485 1493 Size: 924 581 226 152 2109 1697 Fragments arranged by size: 2109 1697 924 581 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2063 2075 3014 1870 Size: 73 120 12 939 4545 Fragments arranged by size: 4545 939 120 73 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2204 2237 2204 Size: 33 5656 BamHI G'GATC_C Cuts at: 3019 3386 3019 Size: 367 5322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2808 3010 4465 618 Size: 325 217 627 1021 202 1455 1842 Fragments arranged by size: 1842 1455 1021 627 325 217 202 BanII G_rGCy'C Cuts at: 1391 2961 3008 1391 Size: 1570 47 4072 Fragments arranged by size: 4072 1570 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 2199 2430 2912 928 Size: 971 252 48 231 482 3705 Fragments arranged by size: 3705 971 482 252 231 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2325 3581 4791 2325 Size: 1256 1210 3223 Fragments arranged by size: 3223 1256 1210 BclI T'GATC_A Cuts at: 1548 2868 1548 Size: 1320 4369 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2892 5684 2892 Size: 2792 2897 BlpI GC'TnA_GC Cuts at: 2460 2980 3106 2460 Size: 520 126 5043 Fragments arranged by size: 5043 520 126 Bme1580I G_kGCm'C Cuts at: 1790 3750 4906 1790 Size: 1960 1156 2573 Fragments arranged by size: 2573 1960 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2067 2114 2339 2538 2598 2733 2855 4349 Size: 828 47 225 199 60 135 122 1494 Cuts at: 4349 1239 Size: 2579 Fragments arranged by size: 2579 1494 828 225 199 135 122 60 47 Bpu10I CC'TnA_GC Cuts at: 2718 2718 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 3814 4679 4977 2228 Size: 1586 865 298 2940 Fragments arranged by size: 2940 1586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2689 4352 882 Size: 617 1190 1663 2219 Fragments arranged by size: 2219 1663 1190 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 2930 5683 2930 Size: 2753 2936 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2337 2367 1178 Size: 30 165 30 934 30 4500 Fragments arranged by size: 4500 934 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2614 1186 Size: 907 521 4261 Fragments arranged by size: 4261 907 521 BseYI C'CCAG_C Cuts at: 2619 4892 5388 2619 Size: 2273 496 2920 Fragments arranged by size: 2920 2273 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2724 3750 3835 4906 2724 Size: 1026 85 1071 3507 Fragments arranged by size: 3507 1071 1026 85 BsmI GAATG_Cn' Cuts at: 2344 3200 3293 2344 Size: 856 93 4740 Fragments arranged by size: 4740 856 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2724 2961 3008 3750 3835 4906 1391 Size: 399 934 237 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 934 742 399 237 85 47 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2544 3578 5308 2544 Size: 1034 1730 2925 Fragments arranged by size: 2925 1730 1034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2617 3044 844 Size: 1773 427 3489 Fragments arranged by size: 3489 1773 427 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 2973 4333 4520 1700 Size: 110 246 917 1360 187 2869 Fragments arranged by size: 2869 1360 917 246 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 2263 794 Size: 1469 4220 BstBI TT'CG_AA Cuts at: 2763 4493 2763 Size: 1730 3959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2833 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2659 2892 2931 3019 3386 3771 3788 5240 Size: 687 233 39 88 367 385 17 1452 Cuts at: 5240 5326 5338 5684 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 687 385 367 346 233 88 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2013 2739 513 Size: 552 948 726 3463 Fragments arranged by size: 3463 948 726 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2415 3205 3999 4019 2415 Size: 790 794 20 4085 Fragments arranged by size: 4085 794 790 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654 Size: 54 1425 50 275 18 1306 889 1629 Cuts at: 5654 8 Size: 43 Fragments arranged by size: 1629 1425 1306 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2082 2288 2354 2966 3619 1353 Size: 729 206 66 612 653 3423 Fragments arranged by size: 3423 729 653 612 206 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2960 4276 4650 4796 387 Size: 1064 138 1371 1316 374 146 1280 Fragments arranged by size: 1371 1316 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2114 2339 2538 2598 2733 2855 Size: 393 435 47 225 199 60 135 122 Cuts at: 2855 2933 3752 4349 5136 5404 1239 Size: 78 819 597 787 268 1524 Fragments arranged by size: 1524 819 787 597 435 393 268 225 199 135 122 78 60 47 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2054 2103 2980 4836 1678 Size: 376 49 877 1856 2531 Fragments arranged by size: 2531 1856 877 376 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373 Size: 32 135 32 2758 32 42 32 2626 Fragments arranged by size: 2758 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 3033 3279 669 Size: 988 281 699 396 246 3079 Fragments arranged by size: 3079 988 699 396 281 246 HindIII A'AGCT_T Cuts at: 748 2678 748 Size: 1930 3759 HpaI GTT'AAC Cuts at: 2637 3279 2637 Size: 642 5047 KpnI G_GTAC'C Cuts at: 2812 3014 2812 Size: 202 5487 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 4803 4987 1305 Size: 813 2685 184 2007 Fragments arranged by size: 2685 2007 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3867 Fragments arranged by size: 3867 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2924 3647 4006 4165 518 Size: 648 1758 723 359 159 2042 Fragments arranged by size: 2042 1758 723 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2121 2359 2741 2984 3105 3782 4930 Size: 409 106 238 382 243 121 677 1148 Cuts at: 4930 5175 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 409 382 245 243 238 121 106 NaeI GCC'GGC Cuts at: 2058 2975 2058 Size: 917 4772 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2056 2973 2056 Size: 917 4772 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2337 2369 3758 3790 5271 5303 2337 Size: 32 1389 32 1481 32 2723 Fragments arranged by size: 2723 1481 1389 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 3041 830 Size: 2211 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2121 2359 2984 2121 Size: 238 625 4826 Fragments arranged by size: 4826 625 238 SacII CC_GC'GG Cuts at: 2016 2742 2016 Size: 726 4963 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SapI GCTCTTCn'nnn_ Cuts at: 2082 2966 2082 Size: 884 4805 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 2069 3937 5649 2069 Size: 1868 1712 2109 Fragments arranged by size: 2109 1868 1712 SfcI C'TryA_G Cuts at: 826 1045 2877 3037 4172 4853 5044 826 Size: 219 1832 160 1135 681 191 1471 Fragments arranged by size: 1832 1471 1135 681 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 2973 1700 Size: 1273 4416 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 2681 3793 4694 4956 820 Size: 197 926 300 438 1112 901 262 1553 Fragments arranged by size: 1553 1112 926 901 438 300 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 3128 513 Size: 552 2063 3074 Fragments arranged by size: 3074 2063 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2704 2887 3716 3733 3886 4071 4516 Size: 1255 1124 183 829 17 153 185 445 Cuts at: 4516 325 Size: 1498 Fragments arranged by size: 1498 1255 1124 829 445 185 183 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2319 2790 Size: 274 80 33 51 1443 90 252 471 Cuts at: 2790 3935 5647 96 Size: 1145 1712 138 Fragments arranged by size: 1712 1443 1145 471 274 252 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2896 2928 5649 5681 2896 Size: 32 2721 32 2904 Fragments arranged by size: 2904 2721 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI