Restriction map for
pFN21AF0034
(Circular) (Six-base) MAPSORT of: pFN21AF0034.seq Check: 2049 from: 1 to: 8641
pFN21AF0034 8641 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2674 3373 2674
Size: 699 7942
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6451 278
Size: 53 83 186 936 4915 2468
Fragments arranged by size:
4915 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5984 1937
Size: 4047 4594
Acc65I G'GTAC_C
Cuts at: 5962 5962
Size: 8641
AclI AA'CG_TT
Cuts at: 1563 6768 7141 1563
Size: 5205 373 3063
Fragments arranged by size:
5205 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4850 5648 6704 8088 8356 1632
Size: 3218 798 1056 1384 268 1917
Fragments arranged by size:
3218 1917 1384 1056 798 268
AfeI AGC'GCT
Cuts at: 3482 3494 5822 3482
Size: 12 2328 6301
Fragments arranged by size:
6301 2328 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8444
AflIII A'CryG_T
Cuts at: 1203 7540 8517 1203
Size: 6337 977 1327
Fragments arranged by size:
6337 1327 977
AgeI A'CCGG_T
Cuts at: 7472 7472
Size: 8641
AhdI GACnn_n'nnGTC
Cuts at: 3343 7370 3343
Size: 4027 4614
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5145 5819 1184
Size: 3961 674 4006
Fragments arranged by size:
4006 3961 674
AlwNI CAG_nnn'CTG
Cuts at: 2463 4998 5383 5453 6384 7956 2463
Size: 2535 385 70 931 1572 3148
Fragments arranged by size:
3148 2535 1572 931 385 70
ApaI G_GGCC'C
Cuts at: 2555 3325 4425 4654 5874 5901 2555
Size: 770 1100 229 1220 27 5295
Fragments arranged by size:
5295 1220 1100 770 229 27
ApaLI G'TGCA_C
Cuts at: 6698 7854 6698
Size: 1156 7485
ApoI r'AATT_y
Cuts at: 1493 4598 5950 6176 6328 8437 1493
Size: 3105 1352 226 152 2109 1697
Fragments arranged by size:
3105 2109 1697 1352 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7195 160
Size: 872 6163 1606
Fragments arranged by size:
6163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8641
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 3030 3326 3810 3877 3951 4149
Size: 73 82 1005 296 484 67 74 198
Cuts at: 4149 4264 4293 5027 5206 5917 5966 1870
Size: 115 29 734 179 711 49 4545
Fragments arranged by size:
4545 1005 734 711 484 296 198 179
115 82 74 73 67 49 29
AvrII C'CTAG_G
Cuts at: 3391 3391
Size: 8641
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2319 2352 2319
Size: 33 8608
BamHI G'GATC_C
Cuts at: 2317 5971 6338 2317
Size: 3654 367 4620
Fragments arranged by size:
4620 3654 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3374 3508 3617 3891 3994
Size: 325 217 627 1587 134 109 274 103
Cuts at: 3994 4257 4408 4868 5962 7417 618
Size: 263 151 460 1094 1455 1842
Fragments arranged by size:
1842 1587 1455 1094 627 460 325 274
263 217 151 134 109 103
BanII G_rGCy'C
Cuts at: 1391 2229 2555 2763 2963 3213 3325 4425 4586
Size: 838 326 208 200 250 112 1100 161
Cuts at: 4586 4654 5874 5901 5960 1391
Size: 68 1220 27 59 4072
Fragments arranged by size:
4072 1220 1100 838 326 250 208 200
161 112 68 59 27
BbeI G_GCGC'C
Cuts at: 3378 3512 3998 4261 4412 3378
Size: 134 486 263 151 7607
Fragments arranged by size:
7607 486 263 151 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 2856 3052 928
Size: 971 140 817 196 6517
Fragments arranged by size:
6517 971 817 196 140
BbvCI CC'TCA_GC
Cuts at: 2394 3303 5408 2394
Size: 909 2105 5627
Fragments arranged by size:
5627 2105 909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6832 6866 1903
Size: 34 4895 34 3678
Fragments arranged by size:
4895 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2320 6533 7743 2320
Size: 4213 1210 3218
Fragments arranged by size:
4213 3218 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3243 3275 3596
Size: 107 122 71 631 605 1571 32 321
Cuts at: 3596 3789 5357 5878 7252 136
Size: 193 1568 521 1374 1525
Fragments arranged by size:
1571 1568 1525 1374 631 605 521 321
193 122 107 71 32
BglII A'GATC_T
Cuts at: 5751 8636 5751
Size: 2885 5756
BlpI GC'TnA_GC
Cuts at: 6058 6058
Size: 8641
Bme1580I G_kGCm'C
Cuts at: 1790 2252 2439 2521 2540 2555 3325 3620 4425
Size: 462 187 82 19 15 770 295 805
Cuts at: 4425 4654 5460 5874 5901 6702 7858 1790
Size: 229 806 414 27 801 1156 2573
Fragments arranged by size:
2573 1156 806 805 801 770 462 414
295 229 187 82 27 19 15
BmgBI CAC'GTC
Cuts at: 1318 5568 1318
Size: 4250 4391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5777 6372 7330
Size: 416 53 83 399 24 4360 595 958
Cuts at: 7330 442
Size: 1753
Fragments arranged by size:
4360 1753 958 595 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8641
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 3155 3619 4639 2734
Size: 421 464 1020 6736
Fragments arranged by size:
6736 1020 464 421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2402 2485 5473 7301 1239
Size: 1163 83 2988 1828 2579
Fragments arranged by size:
2988 2579 1828 1163 83
Bpu10I CC'TnA_GC
Cuts at: 2394 2933 3303 4182 4453 5408 2394
Size: 539 370 879 271 955 5627
Fragments arranged by size:
5627 955 879 539 370 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5413 6766 7631 7929 5413
Size: 1353 865 298 6125
Fragments arranged by size:
6125 1353 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2073 2894 3109 4311 5020 7304 882
Size: 617 574 821 215 1202 709 2284 2219
Fragments arranged by size:
2284 2219 1202 821 709 617 574 215
BsaAI yAC'GTr
Cuts at: 493 1204 6432 493
Size: 711 5228 2702
Fragments arranged by size:
5228 2702 711
BsaBI GATnn'nnATC
Cuts at: 5622 8635 5622
Size: 3013 5628
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3375 3509 3995 4258
Size: 53 83 186 936 1842 134 486 263
Cuts at: 4258 4409 4593 5310 6448 6830 7465 275
Size: 151 184 717 1138 382 635 1451
Fragments arranged by size:
1842 1451 1138 936 717 635 486 382
263 186 184 151 134 83 53
BsaWI w'CCGG_w
Cuts at: 2734 4972 7074 7472 7746 7893 8304 2734
Size: 2238 2102 398 274 147 411 3071
Fragments arranged by size:
3071 2238 2102 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2077 2107 2470 2500 2729
Size: 30 165 30 674 30 363 30 229
Cuts at: 2729 2759 4181 4211 4289 4319 5434 5464 1178
Size: 30 1422 30 78 30 1115 30 4355
Fragments arranged by size:
4355 1422 1115 674 363 229 165 78
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2491 2566 2578 2822 2831 2834 2870
Size: 868 437 75 12 244 9 3 36
Cuts at: 2870 2873 2876 3218 3221 4165 4238 4267 4270
Size: 3 3 342 3 944 73 29 3
Cuts at: 4270 4273 4276 4898 4919 5045 5057 5084 5087
Size: 3 3 622 21 126 12 27 3
Cuts at: 5087 5090 5093 5108 5111 5126 5129 5132 5675
Size: 3 3 15 3 15 3 3 543
Cuts at: 5675 5729 1186
Size: 54 4098
Fragments arranged by size:
4098 944 868 622 543 437 342 244
126 75 73 54 36 29 27 21
15 15 12 12 9 3 3 3
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2339 2438 2656 2777 3291 3432 3621 5048 5180
Size: 99 218 121 514 141 189 1427 132
Cuts at: 5180 5241 5446 7844 8340 2339
Size: 61 205 2398 496 2640
Fragments arranged by size:
2640 2398 1427 514 496 218 205 189
141 132 121 99 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2634 5281 5310 5621 1163
Size: 573 898 2647 29 311 4183
Fragments arranged by size:
4183 2647 898 573 311 29
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 3270 3528 3778 3944 5848
Size: 275 186 1246 23 258 250 166 1904
Cuts at: 5848 5882 6091 6852 7880 1540
Size: 34 209 761 1028 2301
Fragments arranged by size:
2301 1904 1246 1028 761 275 258 250
209 186 166 34 23
BsiHKAI G_wGCw'C
Cuts at: 6702 6787 7858 6702
Size: 85 1071 7485
Fragments arranged by size:
7485 1071 85
BsmI GAATG_Cn'
Cuts at: 6152 6245 6152
Size: 93 8548
BsmBI CGTCTCn'nnnn_
Cuts at: 2459 2538 2459
Size: 79 8562
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2252 2439 2521 2540 2555 2763
Size: 399 439 23 187 82 19 15 208
Cuts at: 2763 2963 3213 3325 3620 4425 4586 4654 5460
Size: 200 250 112 295 805 161 68 806
Cuts at: 5460 5874 5901 5960 6702 6787 7858 1391
Size: 414 27 59 742 85 1071 2174
Fragments arranged by size:
2174 1071 806 805 742 439 414 399
295 250 208 200 187 161 112 85
82 68 59 27 23 19 15
BspEI T'CCGG_A
Cuts at: 2734 4972 8304 2734
Size: 2238 3332 3071
Fragments arranged by size:
3332 3071 2238
BspHI T'CATG_A
Cuts at: 6530 8260 6530
Size: 1730 6911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2361 2674 3373 3456 3789 5505 5996
Size: 1319 198 313 699 83 333 1716 491
Cuts at: 5996 844
Size: 3489
Fragments arranged by size:
3489 1716 1319 699 491 333 313 198
83
BsrBI CCG'CTC
Cuts at: 1394 1823 3314 3672 3723 3729 3735 3741 3747
Size: 429 1491 358 51 6 6 6 6
Cuts at: 3747 3762 3889 4218 4228 4263 4648 4909 5650
Size: 15 127 329 10 35 385 261 741
Cuts at: 5650 6528 1394
Size: 878 3507
Fragments arranged by size:
3507 1491 878 741 429 385 358 329
261 127 51 35 15 10 6 6
6 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 7136 7310 65
Size: 1183 5888 174 1396
Fragments arranged by size:
5888 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2765 3051 3265 3400 4128 4574 5198
Size: 110 955 286 214 135 728 446 624
Cuts at: 5198 7285 7472 1700
Size: 2087 187 2869
Fragments arranged by size:
2869 2087 955 728 624 446 286 214
187 135 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8641
BssSI C'ACGA_G
Cuts at: 4024 6701 7713 4024
Size: 2677 1012 4952
Fragments arranged by size:
4952 2677 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8641
BstBI TT'CG_AA
Cuts at: 7445 7445
Size: 8641
BstEII G'GTnAC_C
Cuts at: 1184 3118 4031 4331 1184
Size: 1934 913 300 5494
Fragments arranged by size:
5494 1934 913 300
BstXI CCAn_nnnn'nTGG
Cuts at: 2347 4123 4663 5467 5557 2347
Size: 1776 540 804 90 5431
Fragments arranged by size:
5431 1776 804 540 90
BstYI r'GATC_y
Cuts at: 1972 2317 2416 3459 5403 5751 5971 6338 6723
Size: 345 99 1043 1944 348 220 367 385
Cuts at: 6723 6740 8192 8278 8290 8636 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1944 1452 1043 385 367 348 346
345 220 99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2805 4417 5010 5732 2805
Size: 1612 593 722 5714
Fragments arranged by size:
5714 1612 722 593
BtgI C'CryG_G
Cuts at: 513 1065 2004 2216 2280 3285 3317 3784 3895
Size: 552 939 212 64 1005 32 467 111
Cuts at: 3895 4986 5139 5605 5876 513
Size: 1091 153 466 271 3278
Fragments arranged by size:
3278 1091 1005 939 552 467 466 271
212 153 111 64 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2076 487
Size: 683 56 850 7052
Fragments arranged by size:
7052 850 683 56
BtsI GCAGTG_nn'
Cuts at: 4795 4816 5151 5172 6157 6951 6971 4795
Size: 21 335 21 985 794 20 6465
Fragments arranged by size:
6465 985 794 335 21 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8606
DraI TTT'AAA
Cuts at: 5945 5945
Size: 8641
DraIII CAC_nnn'GTG
Cuts at: 4026 4026
Size: 8641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2041 4894 7648 809
Size: 507 725 2853 2754 1802
Fragments arranged by size:
2853 2754 1802 725 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 3244 3267
Size: 54 1425 50 275 18 447 967 23
Cuts at: 3267 3276 3525 3855 4554 4665 4816 5142 5673
Size: 9 249 330 699 111 151 326 531
Cuts at: 5673 5879 6088 6977 8606 8
Size: 206 209 889 1629 43
Fragments arranged by size:
1629 1425 967 889 699 531 447 330
326 275 249 209 206 151 111 54
50 43 23 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3244 3267 3525 5879 6088 1537
Size: 275 1432 23 258 2354 209 4090
Fragments arranged by size:
4090 2354 1432 275 258 209 23
EarI CTCTTCn'nnn_
Cuts at: 1353 2808 2820 2832 2838 3236 4103 4294 4465
Size: 1455 12 12 6 398 867 191 171
Cuts at: 4465 5085 5097 5103 5339 6571 1353
Size: 620 12 6 236 1232 3423
Fragments arranged by size:
3423 1455 1232 867 620 398 236 191
171 12 12 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2058 3224 3531 3700 3829 4859
Size: 1064 138 469 1166 307 169 129 1030
Cuts at: 4859 4895 5081 5228 7228 7602 7748 387
Size: 36 186 147 2000 374 146 1280
Fragments arranged by size:
2000 1280 1166 1064 1030 469 374 307
186 169 147 146 138 129 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2402 2485 4850 5473 5648 6704 7301
Size: 393 770 83 2365 623 175 1056 597
Cuts at: 7301 8088 8356 1239
Size: 787 268 1524
Fragments arranged by size:
2365 1524 1056 787 770 623 597 393
268 175 83
EcoNI CCTnn'n_nnAGG
Cuts at: 2936 4416 5027 5735 2936
Size: 1480 611 708 5842
Fragments arranged by size:
5842 1480 708 611
EcoO109I rG'GnC_Cy
Cuts at: 2152 2460 2551 2552 2793 3322 3988 4078 4154
Size: 308 91 1 241 529 666 90 76
Cuts at: 4154 4192 4421 4422 4651 5860 5870 5871 5897
Size: 38 229 1 229 1209 10 1 26
Cuts at: 5897 5898 2152
Size: 1 4895
Fragments arranged by size:
4895 1209 666 529 308 241 229 229
91 90 76 38 26 10 1 1
1 1
EcoRI G'AATT_C
Cuts at: 5950 6328 5950
Size: 378 8263
EcoRV GAT'ATC
Cuts at: 2683 2683
Size: 8641
FspI TGC'GCA
Cuts at: 7147 7147
Size: 8641
HaeII r_GCGC'y
Cuts at: 1678 2206 3224 3378 3484 3496 3512 3632 3838
Size: 528 1018 154 106 12 16 120 206
Cuts at: 3838 3998 4261 4322 4412 5824 7788 1678
Size: 160 263 61 90 1412 1964 2531
Fragments arranged by size:
2531 1964 1412 1018 528 263 206 160
154 120 106 90 61 16 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2406 2438 2667 2699 3329
Size: 32 135 32 834 32 229 32 630
Cuts at: 3329 3361 3950 3982 4289 4321 4361 4393 4553
Size: 32 589 32 307 32 40 32 160
Cuts at: 4553 4585 5434 5466 7282 7314 7356 7388 1373
Size: 32 849 32 1816 32 42 32 2626
Fragments arranged by size:
2626 1816 849 834 630 589 307 229
160 135 42 40 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5985 6231 669
Size: 988 281 4047 246 3079
Fragments arranged by size:
4047 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8641
HpaI GTT'AAC
Cuts at: 6231 6231
Size: 8641
KasI G'GCGC_C
Cuts at: 3374 3508 3994 4257 4408 3374
Size: 134 486 263 151 7607
Fragments arranged by size:
7607 486 263 151 134
KpnI G_GTAC'C
Cuts at: 5966 5966
Size: 8641
MfeI C'AATT_G
Cuts at: 6240 6240
Size: 8641
MluI A'CGCG_T
Cuts at: 8517 8517
Size: 8641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 2796 3342 4167 4292 7755 7939 1305
Size: 945 546 546 825 125 3463 184 2007
Fragments arranged by size:
3463 2007 945 825 546 546 184 125
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 4667 4818 5675 10
Size: 54 1425 343 447 2388 151 857 2976
Fragments arranged by size:
2976 2388 1425 857 447 343 151 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4121 4661 6599 6958 7117 518
Size: 648 2955 540 1938 359 159 2042
Fragments arranged by size:
2955 2042 1938 648 540 359 159
MspA1I CmG'CkG
Cuts at: 1606 2789 3125 3319 3533 3582 3786 3897 4449
Size: 1183 336 194 214 49 204 111 552
Cuts at: 4449 4803 4998 5245 5285 5307 6057 6734 7882
Size: 354 195 247 40 22 750 677 1148
Cuts at: 7882 8127 1606
Size: 245 2120
Fragments arranged by size:
2120 1183 1148 750 677 552 354 336
247 245 214 204 195 194 111 49
40 22
NaeI GCC'GGC
Cuts at: 3053 3402 4130 4576 3053
Size: 349 728 446 7118
Fragments arranged by size:
7118 728 446 349
NarI GG'CG_CC
Cuts at: 3375 3509 3995 4258 4409 3375
Size: 134 486 263 151 7607
Fragments arranged by size:
7607 486 263 151 134
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7150
Fragments arranged by size:
7150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8641
NgoMIV G'CCGG_C
Cuts at: 3051 3400 4128 4574 3051
Size: 349 728 446 7118
Fragments arranged by size:
7118 728 446 349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8641
NotI GC'GGCC_GC
Cuts at: 6088 6088
Size: 8641
NspI r_CATG'y
Cuts at: 1132 1229 2380 3356 5999 7544 1132
Size: 97 1151 976 2643 1545 2229
Fragments arranged by size:
2643 2229 1545 1151 976 97
PasI CC'CwG_GG
Cuts at: 1348 3651 5867 1348
Size: 2303 2216 4122
Fragments arranged by size:
4122 2303 2216
PciI A'CATG_T
Cuts at: 7540 7540
Size: 8641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5271 1108
Size: 4163 4478
PfoI T'CCnGG_A
Cuts at: 2313 4074 4487 2313
Size: 1761 413 6467
Fragments arranged by size:
6467 1761 413
PmeI GTTT'AAAC
Cuts at: 5945 5945
Size: 8641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6710 6742 8223 8255 6710
Size: 32 1481 32 7096
Fragments arranged by size:
7096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2152 2793 4078 4154 4192 5860 2152
Size: 641 1285 76 38 1668 4933
Fragments arranged by size:
4933 1668 1285 641 76 38
PshAI GACnn'nnGTC
Cuts at: 1613 4706 1613
Size: 3093 5548
PsiI TTA'TAA
Cuts at: 6211 6211
Size: 8641
PspOMI G'GGCC_C
Cuts at: 2551 3321 4421 4650 5870 5897 2551
Size: 770 1100 229 1220 27 5295
Fragments arranged by size:
5295 1220 1100 770 229 27
PspXI vC'TCGA_Gb
Cuts at: 3877 3877
Size: 8641
PstI C_TGCA'G
Cuts at: 830 2148 3384 4506 4856 5161 5993 830
Size: 1318 1236 1122 350 305 832 3478
Fragments arranged by size:
3478 1318 1236 1122 832 350 305
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8641
PvuII CAG'CTG
Cuts at: 4998 5245 5285 4998
Size: 247 40 8354
Fragments arranged by size:
8354 247 40
SacII CC_GC'GG
Cuts at: 3320 3787 3898 3320
Size: 467 111 8063
Fragments arranged by size:
8063 467 111
SalI G'TCGA_C
Cuts at: 1936 5983 1936
Size: 4047 4594
SanDI GG'GwC_CC
Cuts at: 2793 5860 2793
Size: 3067 5574
SapI GCTCTTCn'nnn_
Cuts at: 4465 4465
Size: 8641
SbfI CC_TGCA'GG
Cuts at: 2148 3384 5993 2148
Size: 1236 2609 4796
Fragments arranged by size:
4796 2609 1236
ScaI AGT'ACT
Cuts at: 6889 8601 6889
Size: 1712 6929
SexAI A'CCwGG_T
Cuts at: 5799 5799
Size: 8641
SfcI C'TryA_G
Cuts at: 826 1045 2144 3380 4502 4852 5157 5989 7124
Size: 219 1099 1236 1122 350 305 832 1135
Cuts at: 7124 7805 7996 826
Size: 681 191 1471
Fragments arranged by size:
1471 1236 1135 1122 1099 832 681 350
305 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3275 3596 5878 3275
Size: 321 2282 6038
Fragments arranged by size:
6038 2282 321
SfoI GGC'GCC
Cuts at: 3376 3510 3996 4259 4410 3376
Size: 134 486 263 151 7607
Fragments arranged by size:
7607 486 263 151 134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8641
SgrAI Cr'CCGG_yG
Cuts at: 1700 3051 1700
Size: 1351 7290
SmaI CCC'GGG
Cuts at: 1872 3032 3812 3953 4295 5919 5968 1872
Size: 1160 780 141 342 1624 49 4545
Fragments arranged by size:
4545 1624 1160 780 342 141 49
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2025 3877 5428 6745 7646 7908
Size: 197 926 82 1852 1551 1317 901 262
Cuts at: 7908 820
Size: 1553
Fragments arranged by size:
1852 1553 1551 1317 926 901 262 197
82
SnaBI TAC'GTA
Cuts at: 493 6432 493
Size: 5939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8641
SphI G_CATG'C
Cuts at: 1132 3356 5999 1132
Size: 2224 2643 3774
Fragments arranged by size:
3774 2643 2224
SrfI GCCC'GGGC
Cuts at: 5919 5919
Size: 8641
SspI AAT'ATT
Cuts at: 5 52 6565 5
Size: 47 6513 2081
Fragments arranged by size:
6513 2081 47
StuI AGG'CCT
Cuts at: 1517 2714 2871 1517
Size: 1197 157 7287
Fragments arranged by size:
7287 1197 157
StyI C'CwwG_G
Cuts at: 513 1065 2004 2532 3391 3591 4398 4509 4557
Size: 552 939 528 859 200 807 111 48
Cuts at: 4557 4704 4716 4737 4746 4839 4872 4917 4941
Size: 147 12 21 9 93 33 45 24
Cuts at: 4941 5709 5886 6080 513
Size: 768 177 194 3074
Fragments arranged by size:
3074 939 859 807 768 552 528 200
194 177 147 111 93 48 45 33
24 21 12 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 3635 6668 6685 6838 7023 7468
Size: 1255 1509 546 3033 17 153 185 445
Cuts at: 7468 325
Size: 1498
Fragments arranged by size:
3033 1509 1498 1255 546 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5718 6887 8599
Size: 274 80 33 51 1443 3741 1169 1712
Cuts at: 8599 96
Size: 138
Fragments arranged by size:
3741 1712 1443 1169 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2077 2109 4056 4088 8601 8633 2077
Size: 32 1947 32 4513 32 2085
Fragments arranged by size:
4513 2085 1947 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8641
XbaI T'CTAG_A
Cuts at: 5977 5977
Size: 8641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5311 5765 5311
Size: 454 8187
XhoI C'TCGA_G
Cuts at: 1943 2025 3877 1943
Size: 82 1852 6707
Fragments arranged by size:
6707 1852 82
XmaI C'CCGG_G
Cuts at: 1870 3030 3810 3951 4293 5917 5966 1870
Size: 1160 780 141 342 1624 49 4545
Fragments arranged by size:
4545 1624 1160 780 342 141 49
XmnI GAAnn'nnTTC
Cuts at: 6770 6770
Size: 8641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6449 276
Size: 53 83 186 936 4915 2468
Fragments arranged by size:
4915 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BsiWI BstZ17I EcoICRI FalI FseI
FspAI NruI NsiI PacI PmlI PsrI RsrII SacI
SwaI