Restriction map for
pFN21AF0006
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccI AccIII AclI AcsI
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbrPI
BbsI BbvCI BciVI BfaI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BssHII Bst1107I
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT22I FseI HincII HindII
HindIII HpaI KspI MaeI MamI
MfeI MluI MroI MunI NcoI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SnaBI SpeI Sse8387I SspI
StuI SwaI TfiI Tsp509I Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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Acc65I 2051 GGTACC G^GTACC
AflII 1702 CTTAAG C^TTAAG
Alw44I 1341 GTGCAC G^TGCAC
ApaLI 1341 GTGCAC G^TGCAC
Asp718I 2051 GGTACC G^GTACC
BclI 824 TGATCA T^GATCA
BfrI 1702 CTTAAG C^TTAAG
BglII 68 AGATCT A^GATCT
BsaAI 1420 TACGTG [CT]AC^GT[GA]
BsaI 454 GAGACC GGTCTC(1/5)
BsmBI 294 CGTCTC CGTCTC(1/5)
Bsp1407I 146 TGTACA T^GTACA
BsrDI 1131 CATTGC GCAATG(2/0)
BsrGI 146 TGTACA T^GTACA
BstBI 1714 TTCGAA TT^CGAA
DraIII 259 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 739 GACCAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1015 GATATC GAT^ATC
EcoT14I 1454 CCAAGG C^C[AT][AT]GG
FbaI 824 TGATCA T^GATCA
KpnI 2051 GGTACC GGTAC^C
MseI 1703 TTAA T^TAA
NdeI 54 CATATG CA^TATG
NspV 1714 TTCGAA TT^CGAA
ScaI 1628 AGTACT AGT^ACT
SfiI 1698 GGCCCTTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1714 TTCGAA TT^CGAA
SgrAI 1441 CACCGGCG C[GA]^CCGG[CT]G
SspBI 146 TGTACA T^GTACA
StyI 1454 CCAAGG C^C[AT][AT]GG
Tru9I 1703 TTAA T^TAA
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# TWO CUT ENZYMES ( 28 enzymes) #
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AlwI 972 GATCC GGATC(4/5)
270 GGATC
ApaI 386 GGGCCC GGGCC^C
566 GGGCCC
AvaI 917 CCCGGG C^[CT]CG[GA]G
2079 CCCGGG
BalI 74 TGGCCA TGG^CCA
1058 TGGCCA
BbeI 1445 GGCGCC GGCGC^C
1750 GGCGCC
BcgI 1776 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2110 CGAGTCCTCTGC
BglI 1571 GCCTGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1699 GCCCTTAAGGC
BsoBI 917 CCCGGG C^[CT]CG[GA]G
2079 CCCGGG
BssSI 1411 CACGAG CACGAG(-5/-1)
1675 CTCGTG
BstYI 68 AGATCT [GA]^GATC[CT]
270 GGATCT
DsaI 1094 CCGTGG C^C[GA][CT]GG
2039 CCGTGG
KasI 1445 GGCGCC G^GCGCC
1750 GGCGCC
MaeII 1421 ACGT A^CGT
1919 ACGT
MflI 68 AGATCT [GA]^GATC[CT]
270 GGATCT
MluNI 74 TGGCCA TGG^CCA
1058 TGGCCA
MscI 74 TGGCCA TGG^CCA
1058 TGGCCA
NarI 1445 GGCGCC GG^CGCC
1750 GGCGCC
PflMI 258 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1146 CCAGCTCCTGG
PvuII 364 CAGCTG CAG^CTG
2229 CAGCTG
SapI 964 GAAGAGC GCTCTTC(1/4)
1233 GCTCTTC
SfoI 1445 GGCGCC GGC^GCC
1750 GGCGCC
SmaI 917 CCCGGG CCC^GGG
2079 CCCGGG
SphI 944 GCATGC GCATG^C
1938 GCATGC
Van91I 258 CCACGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1146 CCAGCTCCTGG
XcmI 1313 CCATCCTGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1454 CCAAGGCCCTGCTGG
XhoII 68 AGATCT [GA]^GATC[CT]
270 GGATCT
XmaCI 917 CCCGGG C^CCGGG
2079 CCCGGG
XmaI 917 CCCGGG C^CCGGG
2079 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII AviII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI Tsp45I TspRI
TthHB8I