Restriction map for
pFN21AF0006
(Linear) (Six-base) MAPSORT of: FLJ00006.seq Check: 7233 from: 1 to: 2247
FLJ00006.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 1983 2247
Size: 27 1956 264
Fragments arranged by size:
1956 264 27
Acc65I G'GTAC_C
Cuts at: 0 2051 2247
Size: 2051 196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 1747 1833 2115 2206 2211 2247
Size: 693 1054 86 282 91 5 36
Fragments arranged by size:
1054 693 282 91 86 36 5
AflII C'TTAA_G
Cuts at: 0 1702 2247
Size: 1702 545
AleI CACnn'nnGTG
Cuts at: 0 366 1087 1525 2247
Size: 366 721 438 722
Fragments arranged by size:
722 721 438 366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 736 2247
Size: 556 180 1511
Fragments arranged by size:
1511 556 180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 976 1008 2247
Size: 976 32 1239
Fragments arranged by size:
1239 976 32
AlwNI CAG_nnn'CTG
Cuts at: 0 222 774 1152 1998 2184 2247
Size: 222 552 378 846 186 63
Fragments arranged by size:
846 552 378 222 186 63
ApaI G_GGCC'C
Cuts at: 0 390 570 2247
Size: 390 180 1677
Fragments arranged by size:
1677 390 180
ApaLI G'TGCA_C
Cuts at: 0 1341 2247
Size: 1341 906
AvaI C'yCGr_G
Cuts at: 0 917 2079 2247
Size: 917 1162 168
Fragments arranged by size:
1162 917 168
BanI G'GyrC_C
Cuts at: 0 540 607 1162 1445 1750 2051 2247
Size: 540 67 555 283 305 301 196
Fragments arranged by size:
555 540 305 301 283 196 67
BanII G_rGCy'C
Cuts at: 0 390 570 916 1565 1985 2108 2156 2247
Size: 390 180 346 649 420 123 48 91
Fragments arranged by size:
649 420 390 346 180 123 91 48
BbeI G_GCGC'C
Cuts at: 0 1449 1754 2247
Size: 1449 305 493
Fragments arranged by size:
1449 493 305
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1765 1799 2099 2133 2247
Size: 1765 34 300 34 114
Fragments arranged by size:
1765 300 114 34 34
BclI T'GATC_A
Cuts at: 0 824 2247
Size: 824 1423
BglI GCCn_nnn'nGGC
Cuts at: 0 1577 1705 2247
Size: 1577 128 542
Fragments arranged by size:
1577 542 128
BglII A'GATC_T
Cuts at: 0 68 2247
Size: 68 2179
Bme1580I G_kGCm'C
Cuts at: 0 390 545 570 610 1345 2247
Size: 390 155 25 40 735 902
Fragments arranged by size:
902 735 390 155 40 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 2157 2247
Size: 2157 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 189 1705 2247
Size: 189 1516 542
Fragments arranged by size:
1516 542 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 262 664 781 1273 1361 2010 2032
Size: 159 103 402 117 492 88 649 22
Cuts at: 2032 2247
Size: 215
Fragments arranged by size:
649 492 402 215 159 117 103 88
22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 940 1246 2247
Size: 940 306 1001
Fragments arranged by size:
1001 940 306
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 2247
Size: 448 1799
BsaAI yAC'GTr
Cuts at: 0 1422 2247
Size: 1422 825
BsaHI Gr'CG_yC
Cuts at: 0 656 1446 1751 2195 2247
Size: 656 790 305 444 52
Fragments arranged by size:
790 656 444 305 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 898 928 976 1006 1798 1828 2035 2065
Size: 898 30 48 30 792 30 207 30
Cuts at: 2065 2247
Size: 182
Fragments arranged by size:
898 792 207 182 48 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 664 1792 1900 2247
Size: 661 3 1128 108 347
Fragments arranged by size:
1128 661 347 108 3
BseYI C'CCAG_C
Cuts at: 0 502 723 939 1202 1494 1550 2084 2231
Size: 502 221 216 263 292 56 534 147
Cuts at: 2231 2247
Size: 16
Fragments arranged by size:
534 502 292 263 221 216 147 56
16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 934 1927 1984 2247
Size: 817 117 993 57 263
Fragments arranged by size:
993 817 263 117 57
BsiHKAI G_wGCw'C
Cuts at: 0 563 1345 1432 1547 1565 1838 2021 2108
Size: 563 782 87 115 18 273 183 87
Cuts at: 2108 2156 2247
Size: 48 91
Fragments arranged by size:
782 563 273 183 115 91 87 87
48 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 300 2247
Size: 300 1947
Bsp1286I G_dGCh'C
Cuts at: 0 390 545 563 570 610 916 1345 1432
Size: 390 155 18 7 40 306 429 87
Cuts at: 1432 1547 1565 1838 1985 2021 2108 2156 2247
Size: 115 18 273 147 36 87 48 91
Fragments arranged by size:
429 390 306 273 155 147 115 91
87 87 48 40 36 18 18 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 494 698 782 1983 2247
Size: 27 467 204 84 1201 264
Fragments arranged by size:
1201 467 264 204 84 27
BsrBI CCG'CTC
Cuts at: 0 308 323 1476 1964 2247
Size: 308 15 1153 488 283
Fragments arranged by size:
1153 488 308 283 15
BsrDI GCAATG_nn'
Cuts at: 0 1130 2247
Size: 1130 1117
BsrFI r'CCGG_y
Cuts at: 0 337 610 1072 1159 1442 2247
Size: 337 273 462 87 283 805
Fragments arranged by size:
805 462 337 283 273 87
BsrGI T'GTAC_A
Cuts at: 0 146 2247
Size: 146 2101
BssSI C'ACGA_G
Cuts at: 0 1411 1675 2247
Size: 1411 264 572
Fragments arranged by size:
1411 572 264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 512 1939 1998 2247
Size: 512 1427 59 249
Fragments arranged by size:
1427 512 249 59
BstBI TT'CG_AA
Cuts at: 0 1715 2247
Size: 1715 532
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 368 723 864 1068 1599 2247
Size: 183 185 355 141 204 531 648
Fragments arranged by size:
648 531 355 204 185 183 141
BstYI r'GATC_y
Cuts at: 0 68 270 2247
Size: 68 202 1977
Fragments arranged by size:
1977 202 68
BtgI C'CryG_G
Cuts at: 0 1094 2039 2247
Size: 1094 945 208
Fragments arranged by size:
1094 945 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 444 1620 2247
Size: 444 1176 627
Fragments arranged by size:
1176 627 444
BtsI GCAGTG_nn'
Cuts at: 0 829 1223 1545 1940 2247
Size: 829 394 322 395 307
Fragments arranged by size:
829 395 394 322 307
DraIII CAC_nnn'GTG
Cuts at: 0 264 2247
Size: 264 1983
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 2247
Size: 745 1502
EaeI y'GGCC_r
Cuts at: 0 74 335 620 1058 1070 1091 2247
Size: 74 261 285 438 12 21 1156
Fragments arranged by size:
1156 438 285 261 74 21 12
EarI CTCTTCn'nnn_
Cuts at: 0 89 394 959 1240 2158 2230 2247
Size: 89 305 565 281 918 72 17
Fragments arranged by size:
918 565 305 281 89 72 17
EcoICRI GAG'CTC
Cuts at: 0 1563 2106 2154 2247
Size: 1563 543 48 93
Fragments arranged by size:
1563 543 93 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 262 664 693 781 1273 1361 1747
Size: 159 103 402 29 88 492 88 386
Cuts at: 1747 1833 2010 2032 2115 2206 2211 2247
Size: 86 177 22 83 91 5 36
Fragments arranged by size:
492 402 386 177 159 103 91 88
88 86 83 36 29 22 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 958 1180 2140 2188 2247
Size: 646 312 222 960 48 59
Fragments arranged by size:
960 646 312 222 59 48
EcoO109I rG'GnC_Cy
Cuts at: 0 386 387 483 567 687 747 894 1199
Size: 386 1 96 84 120 60 147 305
Cuts at: 1199 1458 1685 1707 2247
Size: 259 227 22 540
Fragments arranged by size:
540 386 305 259 227 147 120 96
84 60 22 1
EcoRV GAT'ATC
Cuts at: 0 1017 2247
Size: 1017 1230
FspI TGC'GCA
Cuts at: 0 553 886 2074 2247
Size: 553 333 1188 173
Fragments arranged by size:
1188 553 333 173
HaeII r_GCGC'y
Cuts at: 0 1110 1449 1754 2247
Size: 1110 339 305 493
Fragments arranged by size:
1110 493 339 305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1798 1830 2247
Size: 1006 32 760 32 417
Fragments arranged by size:
1006 760 417 32 32
KasI G'GCGC_C
Cuts at: 0 1445 1750 2247
Size: 1445 305 497
Fragments arranged by size:
1445 497 305
KpnI G_GTAC'C
Cuts at: 0 2055 2247
Size: 2055 192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1835 2247
Size: 470 1365 412
Fragments arranged by size:
1365 470 412
MscI TGG'CCA
Cuts at: 0 76 1060 2247
Size: 76 984 1187
Fragments arranged by size:
1187 984 76
MslI CAynn'nnrTG
Cuts at: 0 366 538 613 1087 1525 2209 2247
Size: 366 172 75 474 438 684 38
Fragments arranged by size:
684 474 438 366 172 75 38
MspA1I CmG'CkG
Cuts at: 0 366 744 1206 1404 2231 2247
Size: 366 378 462 198 827 16
Fragments arranged by size:
827 462 378 366 198 16
NaeI GCC'GGC
Cuts at: 0 339 1074 1161 2247
Size: 339 735 87 1086
Fragments arranged by size:
1086 735 339 87
NarI GG'CG_CC
Cuts at: 0 1446 1751 2247
Size: 1446 305 496
Fragments arranged by size:
1446 496 305
NdeI CA'TA_TG
Cuts at: 0 55 2247
Size: 55 2192
NgoMIV G'CCGG_C
Cuts at: 0 337 1072 1159 2247
Size: 337 735 87 1088
Fragments arranged by size:
1088 735 337 87
NspI r_CATG'y
Cuts at: 0 948 1942 2208 2247
Size: 948 994 266 39
Fragments arranged by size:
994 948 266 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 264 1152 2247
Size: 264 888 1095
Fragments arranged by size:
1095 888 264
PfoI T'CCnGG_A
Cuts at: 0 641 797 1304 1607 2198 2247
Size: 641 156 507 303 591 49
Fragments arranged by size:
641 591 507 303 156 49
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 976 1008 2247
Size: 976 32 1239
Fragments arranged by size:
1239 976 32
PpuMI rG'GwC_Cy
Cuts at: 0 483 687 747 894 1199 1685 2247
Size: 483 204 60 147 305 486 562
Fragments arranged by size:
562 486 483 305 204 147 60
PspOMI G'GGCC_C
Cuts at: 0 386 566 2247
Size: 386 180 1681
Fragments arranged by size:
1681 386 180
PstI C_TGCA'G
Cuts at: 0 395 1363 1788 2247
Size: 395 968 425 459
Fragments arranged by size:
968 459 425 395
PvuII CAG'CTG
Cuts at: 0 366 2231 2247
Size: 366 1865 16
Fragments arranged by size:
1865 366 16
SacI G_AGCT'C
Cuts at: 0 1565 2108 2156 2247
Size: 1565 543 48 91
Fragments arranged by size:
1565 543 91 48
SanDI GG'GwC_CC
Cuts at: 0 1685 2247
Size: 1685 562
SapI GCTCTTCn'nnn_
Cuts at: 0 959 1240 2247
Size: 959 281 1007
Fragments arranged by size:
1007 959 281
ScaI AGT'ACT
Cuts at: 0 1630 2247
Size: 1630 617
SfcI C'TryA_G
Cuts at: 0 391 1359 1383 1784 2247
Size: 391 968 24 401 463
Fragments arranged by size:
968 463 401 391 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1705 2247
Size: 1705 542
SfoI GGC'GCC
Cuts at: 0 1447 1752 2247
Size: 1447 305 495
Fragments arranged by size:
1447 495 305
SgrAI Cr'CCGG_yG
Cuts at: 0 1442 2247
Size: 1442 805
SmaI CCC'GGG
Cuts at: 0 919 2081 2247
Size: 919 1162 166
Fragments arranged by size:
1162 919 166
SmlI C'TyrA_G
Cuts at: 0 955 1261 1702 2247
Size: 955 306 441 545
Fragments arranged by size:
955 545 441 306
SphI G_CATG'C
Cuts at: 0 948 1942 2247
Size: 948 994 305
Fragments arranged by size:
994 948 305
SrfI GCCC'GGGC
Cuts at: 0 2081 2247
Size: 2081 166
StyI C'CwwG_G
Cuts at: 0 1454 2247
Size: 1454 793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 715 1542 2247
Size: 265 450 827 705
Fragments arranged by size:
827 705 450 265
TatI w'GTAC_w
Cuts at: 0 80 146 1628 2247
Size: 80 66 1482 619
Fragments arranged by size:
1482 619 80 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1319 1351 2247
Size: 1319 32 896
Fragments arranged by size:
1319 896 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1320 1461 2247
Size: 1320 141 786
Fragments arranged by size:
1320 786 141
XmaI C'CCGG_G
Cuts at: 0 917 2079 2247
Size: 917 1162 168
Fragments arranged by size:
1162 917 168
Enzymes that do cut:
AarI Acc65I AcuI AflII AleI AlfI AloI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BcgI BclI
BglI BglII Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SanDI SapI ScaI
SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BciVI BfrBI BlpI BmgBI BmtI Bpu10I BsaBI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraI EagI EciI EcoRI FalI
FseI FspAI HincII HindIII HpaI MfeI MluI NcoI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI SexAI SgfI SnaBI SpeI SspI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI