Restriction map for
pFN21AB9877
(Circular) (Six-base) MAPSORT of: pFN21AB9877.seq Check: 3020 from: 1 to: 6343
pFN21AB9877 6343 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2038 2038
Size: 6343
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4153 278
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3686 1937
Size: 1749 4594
Acc65I G'GTAC_C
Cuts at: 3664 3664
Size: 6343
AclI AA'CG_TT
Cuts at: 1563 4470 4843 1563
Size: 2907 373 3063
Fragments arranged by size:
3063 2907 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 4406 5790 6058 1632
Size: 665 2109 1384 268 1917
Fragments arranged by size:
2109 1917 1384 665 268
AfeI AGC'GCT
Cuts at: 2284 2297 2284
Size: 13 6330
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6146
AflIII A'CryG_T
Cuts at: 1203 5242 6219 1203
Size: 4039 977 1327
Fragments arranged by size:
4039 1327 977
AgeI A'CCGG_T
Cuts at: 5174 5174
Size: 6343
AhdI GACnn_n'nnGTC
Cuts at: 5072 5072
Size: 6343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6343
AlwNI CAG_nnn'CTG
Cuts at: 3283 4086 5658 3283
Size: 803 1572 3968
Fragments arranged by size:
3968 1572 803
ApaI G_GGCC'C
Cuts at: 2555 2555
Size: 6343
ApaLI G'TGCA_C
Cuts at: 4400 5556 4400
Size: 1156 5187
ApoI r'AATT_y
Cuts at: 1493 3652 3878 4030 6139 1493
Size: 2159 226 152 2109 1697
Fragments arranged by size:
2159 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4897 160
Size: 872 3865 1606
Fragments arranged by size:
3865 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
AvaI C'yCGr_G
Cuts at: 1870 1943 2434 2546 2570 2974 3162 3270 3491
Size: 73 491 112 24 404 188 108 221
Cuts at: 3491 3668 1870
Size: 177 4545
Fragments arranged by size:
4545 491 404 221 188 177 112 108
73 24
AvrII C'CTAG_G
Cuts at: 3038 3038
Size: 6343
BamHI G'GATC_C
Cuts at: 3673 4040 3673
Size: 367 5976
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2788 3004 3034 3547 3664
Size: 325 217 627 1001 216 30 513 117
Cuts at: 3664 5119 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1001 627 513 325 217 216
117 30
BanII G_rGCy'C
Cuts at: 1391 2369 2457 2555 2562 2814 2981 3662 1391
Size: 978 88 98 7 252 167 681 4072
Fragments arranged by size:
4072 978 681 252 167 98 88 7
BbeI G_GCGC'C
Cuts at: 3551 3551
Size: 6343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2794 928
Size: 971 895 4477
Fragments arranged by size:
4477 971 895
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2770 2804 4534 4568 1903
Size: 34 833 34 1730 34 3678
Fragments arranged by size:
3678 1730 833 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4235 5445 4235
Size: 1210 5133
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6343
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4954 136
Size: 107 122 71 631 605 337 2945 1525
Fragments arranged by size:
2945 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6338 6338
Size: 6343
BlpI GC'TnA_GC
Cuts at: 3760 3760
Size: 6343
Bme1580I G_kGCm'C
Cuts at: 1790 2555 2571 2685 2793 4404 5560 1790
Size: 765 16 114 108 1611 1156 2573
Fragments arranged by size:
2573 1611 1156 765 114 108 16
BmgBI CAC'GTC
Cuts at: 1318 3067 1318
Size: 1749 4594
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2439 4074 5032
Size: 416 53 83 399 24 1022 1635 958
Cuts at: 5032 442
Size: 1753
Fragments arranged by size:
1753 1635 1022 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6343
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2388 2388
Size: 6343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2903 2965 2984 3182 5003 1239
Size: 1664 62 19 198 1821 2579
Fragments arranged by size:
2579 1821 1664 198 62 19
Bpu10I CC'TnA_GC
Cuts at: 2382 2556 2607 2382
Size: 174 51 6118
Fragments arranged by size:
6118 174 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4468 5333 5631 4468
Size: 865 298 5180
Fragments arranged by size:
5180 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2419 2445 5006 882
Size: 617 920 26 2561 2219
Fragments arranged by size:
2561 2219 920 617 26
BsaAI yAC'GTr
Cuts at: 493 1204 2188 3317 4134 493
Size: 711 984 1129 817 2702
Fragments arranged by size:
2702 1129 984 817 711
BsaBI GATnn'nnATC
Cuts at: 6337 6337
Size: 6343
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3054 3116 3267 3377
Size: 53 83 186 936 1521 62 151 110
Cuts at: 3377 3548 4150 4532 5167 275
Size: 171 602 382 635 1451
Fragments arranged by size:
1521 1451 936 635 602 382 186 171
151 110 83 62 53
BsaWI w'CCGG_w
Cuts at: 3049 4776 5174 5448 5595 6006 3049
Size: 1727 398 274 147 411 3386
Fragments arranged by size:
3386 1727 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2963 2993 3218 3248 1178
Size: 30 165 30 1560 30 225 30 4273
Fragments arranged by size:
4273 1560 225 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2291 1186
Size: 1105 5238
BseYI C'CCAG_C
Cuts at: 2488 2623 2792 3329 5546 6042 2488
Size: 135 169 537 2217 496 2789
Fragments arranged by size:
2789 2217 537 496 169 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5770
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2760 3337 3793 4554 5582 1540
Size: 275 186 759 577 456 761 1028 2301
Fragments arranged by size:
2301 1028 761 759 577 456 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 2388 2814 3231 4404 4489 5560 2238
Size: 150 426 417 1173 85 1071 3021
Fragments arranged by size:
3021 1173 1071 426 417 150 85
BsmI GAATG_Cn'
Cuts at: 3854 3947 3854
Size: 93 6250
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2238 2369 2388 2457 2555 2562 2571
Size: 399 448 131 19 69 98 7 9
Cuts at: 2571 2685 2793 2814 2981 3231 3662 4404 4489
Size: 114 108 21 167 250 431 742 85
Cuts at: 4489 5560 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 448 431 399 250 167
131 114 108 98 85 69 21 19
9 7
BspEI T'CCGG_A
Cuts at: 3049 6006 3049
Size: 2957 3386
BspHI T'CATG_A
Cuts at: 4232 5962 4232
Size: 1730 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2038 3261 3698 844
Size: 1194 1223 437 3489
Fragments arranged by size:
3489 1223 1194 437
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 2531 2606 3519 4230 1394
Size: 429 497 211 75 913 711 3507
Fragments arranged by size:
3507 913 711 497 429 211 75
BsrDI GCAATG_nn'
Cuts at: 65 1248 3190 4838 5012 65
Size: 1183 1942 1648 174 1396
Fragments arranged by size:
1942 1648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2759 2965 3537 4987 5174 1700
Size: 110 949 206 572 1450 187 2869
Fragments arranged by size:
2869 1450 949 572 206 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6343
BssHII G'CGCG_C
Cuts at: 1925 2029 1925
Size: 104 6239
BssSI C'ACGA_G
Cuts at: 2237 3230 4403 5415 2237
Size: 993 1173 1012 3165
Fragments arranged by size:
3165 1173 1012 993
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3283 794
Size: 2489 3854
BstBI TT'CG_AA
Cuts at: 5147 5147
Size: 6343
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6343
BstXI CCAn_nnnn'nTGG
Cuts at: 3586 3586
Size: 6343
BstYI r'GATC_y
Cuts at: 1972 3673 4040 4425 4442 5894 5980 5992 6338
Size: 1701 367 385 17 1452 86 12 346
Cuts at: 6338 1972
Size: 1977
Fragments arranged by size:
1977 1701 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2274 2274
Size: 6343
BtgI C'CryG_G
Cuts at: 513 1065 2004 2695 3552 513
Size: 552 939 691 857 3304
Fragments arranged by size:
3304 939 857 691 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5604
Fragments arranged by size:
5604 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 3029 3859 4653 4673 2040
Size: 989 830 794 20 3710
Fragments arranged by size:
3710 989 830 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6343
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6308
DraI TTT'AAA
Cuts at: 3647 3647
Size: 6343
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5350 809
Size: 507 4034 1802
Fragments arranged by size:
4034 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2757 3334
Size: 54 1425 50 275 18 228 699 577
Cuts at: 3334 3369 3600 3790 4679 6308 8
Size: 35 231 190 889 1629 43
Fragments arranged by size:
1629 1425 889 699 577 275 231 228
190 54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 2757 3334 3790 1537
Size: 275 945 577 456 4090
Fragments arranged by size:
4090 945 577 456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2316 2581 4273 1353
Size: 963 265 1692 3423
Fragments arranged by size:
3423 1692 963 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2098 2153 2761 4930 5304 5450
Size: 1064 138 509 55 608 2169 374 146
Cuts at: 5450 387
Size: 1280
Fragments arranged by size:
2169 1280 1064 608 509 374 146 138
55
EcoICRI GAG'CTC
Cuts at: 2812 2812
Size: 6343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2903 2965 2984 3182 4406 5003
Size: 393 665 606 62 19 198 1224 597
Cuts at: 5003 5790 6058 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1224 787 665 606 597 393 268
198 62 19
EcoO109I rG'GnC_Cy
Cuts at: 2269 2512 2551 2552 2818 3076 2269
Size: 243 39 1 266 258 5536
Fragments arranged by size:
5536 266 258 243 39 1
EcoRI G'AATT_C
Cuts at: 3652 4030 3652
Size: 378 5965
FspI TGC'GCA
Cuts at: 4849 4849
Size: 6343
HaeII r_GCGC'y
Cuts at: 1678 2170 2286 2299 2476 2766 3551 5490 1678
Size: 492 116 13 177 290 785 1939 2531
Fragments arranged by size:
2531 1939 785 492 290 177 116 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2843 2875 3106 3138 3218
Size: 32 135 32 1271 32 231 32 80
Cuts at: 3218 3250 4984 5016 5058 5090 1373
Size: 32 1734 32 42 32 2626
Fragments arranged by size:
2626 1734 1271 231 135 80 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3687 3933 669
Size: 988 281 1749 246 3079
Fragments arranged by size:
3079 1749 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6343
HpaI GTT'AAC
Cuts at: 3933 3933
Size: 6343
KasI G'GCGC_C
Cuts at: 3547 3547
Size: 6343
KpnI G_GTAC'C
Cuts at: 3668 3668
Size: 6343
MfeI C'AATT_G
Cuts at: 3942 3942
Size: 6343
MluI A'CGCG_T
Cuts at: 6219 6219
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2543 5457 5641 1305
Size: 1238 2914 184 2007
Fragments arranged by size:
2914 2007 1238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 3371 3602 10
Size: 54 1425 343 228 1311 231 2751
Fragments arranged by size:
2751 1425 1311 343 231 228 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4301 4660 4819 518
Size: 648 3135 359 159 2042
Fragments arranged by size:
3135 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2492 2564 2697 3280 3333 3422 3554 3595
Size: 886 72 133 583 53 89 132 41
Cuts at: 3595 3759 4436 5584 5829 1606
Size: 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 886 677 583 245 164 133
132 89 72 53 41
NaeI GCC'GGC
Cuts at: 2761 2761
Size: 6343
NarI GG'CG_CC
Cuts at: 3548 3548
Size: 6343
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4852
Fragments arranged by size:
4852 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6343
NgoMIV G'CCGG_C
Cuts at: 2759 2759
Size: 6343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6343
NotI GC'GGCC_GC
Cuts at: 3790 3790
Size: 6343
NruI TCG'CGA
Cuts at: 2451 2451
Size: 6343
NspI r_CATG'y
Cuts at: 1132 1229 3701 5246 1132
Size: 97 2472 1545 2229
Fragments arranged by size:
2472 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2650 1348
Size: 1302 5041
PciI A'CATG_T
Cuts at: 5242 5242
Size: 6343
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2188 1108
Size: 1080 5263
PfoI T'CCnGG_A
Cuts at: 2727 2919 3198 3392 2727
Size: 192 279 194 5678
Fragments arranged by size:
5678 279 194 192
PmeI GTTT'AAAC
Cuts at: 3647 3647
Size: 6343
PmlI CAC'GTG
Cuts at: 2188 3317 2188
Size: 1129 5214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4412 4444 5925 5957 4412
Size: 32 1481 32 4798
Fragments arranged by size:
4798 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2269 2512 2818 3076 2269
Size: 243 306 258 5536
Fragments arranged by size:
5536 306 258 243
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6343
PsiI TTA'TAA
Cuts at: 3913 3913
Size: 6343
PspOMI G'GGCC_C
Cuts at: 2551 2551
Size: 6343
PstI C_TGCA'G
Cuts at: 830 2049 2754 3415 3695 830
Size: 1219 705 661 280 3478
Fragments arranged by size:
3478 1219 705 661 280
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6343
PvuII CAG'CTG
Cuts at: 2492 2492
Size: 6343
SacI G_AGCT'C
Cuts at: 2814 2814
Size: 6343
SacII CC_GC'GG
Cuts at: 2698 3555 2698
Size: 857 5486
SalI G'TCGA_C
Cuts at: 1936 3685 1936
Size: 1749 4594
SanDI GG'GwC_CC
Cuts at: 2512 2818 3076 2512
Size: 306 258 5779
Fragments arranged by size:
5779 306 258
SapI GCTCTTCn'nnn_
Cuts at: 2316 2316
Size: 6343
SbfI CC_TGCA'GG
Cuts at: 3695 3695
Size: 6343
ScaI AGT'ACT
Cuts at: 4591 6303 4591
Size: 1712 4631
SfcI C'TryA_G
Cuts at: 826 1045 2045 2750 3411 3691 4826 5507 5698
Size: 219 1000 705 661 280 1135 681 191
Cuts at: 5698 826
Size: 1471
Fragments arranged by size:
1471 1135 1000 705 681 661 280 219
191
SfoI GGC'GCC
Cuts at: 3549 3549
Size: 6343
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6343
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6343
SmaI CCC'GGG
Cuts at: 1872 2572 3670 1872
Size: 700 1098 4545
Fragments arranged by size:
4545 1098 700
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3162 4447 5348 5610 820
Size: 197 926 1219 1285 901 262 1553
Fragments arranged by size:
1553 1285 1219 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4134 493
Size: 3641 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6343
SphI G_CATG'C
Cuts at: 1132 3701 1132
Size: 2569 3774
SspI AAT'ATT
Cuts at: 5 52 2093 4267 5
Size: 47 2041 2174 2081
Fragments arranged by size:
2174 2081 2041 47
StuI AGG'CCT
Cuts at: 1517 2330 2831 2986 1517
Size: 813 501 155 4874
Fragments arranged by size:
4874 813 501 155
StyI C'CwwG_G
Cuts at: 513 1065 2004 3038 3603 3782 513
Size: 552 939 1034 565 179 3074
Fragments arranged by size:
3074 1034 939 565 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2259 4370 4387 4540 4725 5170 325
Size: 1255 679 2111 17 153 185 445 1498
Fragments arranged by size:
2111 1498 1255 679 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2970 4589 6301
Size: 274 80 33 51 1443 993 1619 1712
Cuts at: 6301 96
Size: 138
Fragments arranged by size:
1712 1619 1443 993 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2675 2707 2934 2966 6303 6335 2675
Size: 32 227 32 3337 32 2683
Fragments arranged by size:
3337 2683 227 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6343
XbaI T'CTAG_A
Cuts at: 3679 3679
Size: 6343
XhoI C'TCGA_G
Cuts at: 1943 3162 1943
Size: 1219 5124
XmaI C'CCGG_G
Cuts at: 1870 2570 3668 1870
Size: 700 1098 4545
Fragments arranged by size:
4545 1098 700
XmnI GAAnn'nnTTC
Cuts at: 3099 4472 3099
Size: 1373 4970
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4151 276
Size: 53 83 186 936 2617 2468
Fragments arranged by size:
2617 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I
DraIII EcoNI EcoRV FalI FseI FspAI NsiI PacI
PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI