Restriction map for
pFN21AB9875
(Linear) (Six-base) MAPSORT of: KIBB9875.seq Check: 6478 from: 1 to: 1581
KIBB9875.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
Acc65I G'GTAC_C
Cuts at: 0 1290 1581
Size: 1290 291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 422 583 1073 1163 1581
Size: 190 232 161 490 90 418
Fragments arranged by size:
490 418 232 190 161 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 769 1581
Size: 769 812
ApoI r'AATT_y
Cuts at: 0 66 562 808 893 1147 1192 1581
Size: 66 496 246 85 254 45 389
Fragments arranged by size:
496 389 254 246 85 66 45
AseI AT'TA_AT
Cuts at: 0 425 1581
Size: 425 1156
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1274 1307 1581
Size: 1274 33 274
Fragments arranged by size:
1274 274 33
BanI G'GyrC_C
Cuts at: 0 1290 1581
Size: 1290 291
BanII G_rGCy'C
Cuts at: 0 1313 1461 1581
Size: 1313 148 120
Fragments arranged by size:
1313 148 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 1142 1581
Size: 1142 439
Bme1580I G_kGCm'C
Cuts at: 0 1355 1581
Size: 1355 226
BmrI ACTGGGnnnn_n'
Cuts at: 0 1365 1581
Size: 1365 216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 759 1581
Size: 149 610 822
Fragments arranged by size:
822 610 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 901 1581
Size: 901 680
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 200 1581
Size: 200 1381
BseYI C'CCAG_C
Cuts at: 0 222 732 1581
Size: 222 510 849
Fragments arranged by size:
849 510 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1181 1581
Size: 354 827 400
Fragments arranged by size:
827 400 354
BsiHKAI G_wGCw'C
Cuts at: 0 1313 1461 1581
Size: 1313 148 120
Fragments arranged by size:
1313 148 120
Bsp1286I G_dGCh'C
Cuts at: 0 1313 1355 1461 1581
Size: 1313 42 106 120
Fragments arranged by size:
1313 120 106 42
BsrDI GCAATG_nn'
Cuts at: 0 1440 1581
Size: 1440 141
BsrGI T'GTAC_A
Cuts at: 0 434 698 1581
Size: 434 264 883
Fragments arranged by size:
883 434 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 1581
Size: 429 1152
BstYI r'GATC_y
Cuts at: 0 115 1110 1377 1581
Size: 115 995 267 204
Fragments arranged by size:
995 267 204 115
BtgI C'CryG_G
Cuts at: 0 279 1581
Size: 279 1302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1113 1148 1581
Size: 1113 35 433
Fragments arranged by size:
1113 433 35
EarI CTCTTCn'nnn_
Cuts at: 0 218 1064 1581
Size: 218 846 517
Fragments arranged by size:
846 517 218
EcoICRI GAG'CTC
Cuts at: 0 1311 1459 1581
Size: 1311 148 122
Fragments arranged by size:
1311 148 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 190 422 583 759 1073 1163 1581
Size: 149 41 232 161 176 314 90 418
Fragments arranged by size:
418 314 232 176 161 149 90 41
EcoRV GAT'ATC
Cuts at: 0 1278 1581
Size: 1278 303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1575 1581
Size: 1575 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1581
Size: 595 32 954
Fragments arranged by size:
954 595 32
KpnI G_GTAC'C
Cuts at: 0 1294 1581
Size: 1294 287
MslI CAynn'nnrTG
Cuts at: 0 143 158 427 1346 1581
Size: 143 15 269 919 235
Fragments arranged by size:
919 269 235 143 15
MspA1I CmG'CkG
Cuts at: 0 736 1581
Size: 736 845
NcoI C'CATG_G
Cuts at: 0 279 1581
Size: 279 1302
NspI r_CATG'y
Cuts at: 0 486 1256 1444 1581
Size: 486 770 188 137
Fragments arranged by size:
770 486 188 137
PasI CC'CwG_GG
Cuts at: 0 723 1581
Size: 723 858
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1379 1411 1581
Size: 1379 32 170
Fragments arranged by size:
1379 170 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 380 412 1509 1541 1581
Size: 380 32 1097 32 40
Fragments arranged by size:
1097 380 40 32 32
PstI C_TGCA'G
Cuts at: 0 11 1581
Size: 11 1570
PvuII CAG'CTG
Cuts at: 0 736 1581
Size: 736 845
SacI G_AGCT'C
Cuts at: 0 1313 1461 1581
Size: 1313 148 120
Fragments arranged by size:
1313 148 120
SapI GCTCTTCn'nnn_
Cuts at: 0 1064 1581
Size: 1064 517
SfcI C'TryA_G
Cuts at: 0 7 1581
Size: 7 1574
StyI C'CwwG_G
Cuts at: 0 279 1581
Size: 279 1302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 557 1581
Size: 445 112 1024
Fragments arranged by size:
1024 445 112
TatI w'GTAC_w
Cuts at: 0 434 698 1581
Size: 434 264 883
Fragments arranged by size:
883 434 264
Enzymes that do cut:
Acc65I AcuI AlfI ApoI AseI BaeI BanI BanII
BbsI Bme1580I BmrI BpmI BsaI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrDI BsrGI BstXI BstYI BtgI CspCI
EarI EcoICRI Eco57MI EcoRV FalI Hin4I KpnI MslI
MspA1I NcoI NspI PasI PpiI PsrI PstI PvuII
SacI SapI SfcI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI