(Circular) (Six-base) MAPSORT of: pFN21AB9873.seq Check: 8849 from: 1 to: 6202 pFN21AB9873 6202 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3185 3185 Size: 6202 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4012 278 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3545 1937 Size: 1608 4594 Acc65I G'GTAC_C Cuts at: 2637 3523 2637 Size: 886 5316 AclI AA'CG_TT Cuts at: 1563 4329 4702 1563 Size: 2766 373 3063 Fragments arranged by size: 3063 2766 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2355 4265 5649 5917 1632 Size: 723 1910 1384 268 1917 Fragments arranged by size: 1917 1910 1384 723 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6005 AflIII A'CryG_T Cuts at: 1203 5101 6078 1203 Size: 3898 977 1327 Fragments arranged by size: 3898 1327 977 AgeI A'CCGG_T Cuts at: 3217 5033 3217 Size: 1816 4386 AhdI GACnn_n'nnGTC Cuts at: 3306 4931 3306 Size: 1625 4577 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6202 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6202 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3129 3161 3129 Size: 32 6170 AlwNI CAG_nnn'CTG Cuts at: 2486 2544 3945 5517 2486 Size: 58 1401 1572 3171 Fragments arranged by size: 3171 1572 1401 58 ApaLI G'TGCA_C Cuts at: 4259 5415 4259 Size: 1156 5046 ApoI r'AATT_y Cuts at: 1493 2340 2711 3511 3737 3889 5998 1493 Size: 847 371 800 226 152 2109 1697 Fragments arranged by size: 2109 1697 847 800 371 226 152 AseI AT'TA_AT Cuts at: 160 1032 4756 160 Size: 872 3724 1606 Fragments arranged by size: 3724 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 AvaI C'yCGr_G Cuts at: 1870 1943 3527 1870 Size: 73 1584 4545 Fragments arranged by size: 4545 1584 73 BamHI G'GATC_C Cuts at: 3532 3899 3532 Size: 367 5835 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2055 2637 3523 4978 618 Size: 325 217 627 268 582 886 1455 1842 Fragments arranged by size: 1842 1455 886 627 582 325 268 217 BanII G_rGCy'C Cuts at: 1391 2480 3521 1391 Size: 1089 1041 4072 Fragments arranged by size: 4072 1089 1041 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2589 928 Size: 971 690 4541 Fragments arranged by size: 4541 971 690 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2024 2058 4393 4427 1903 Size: 34 87 34 2335 34 3678 Fragments arranged by size: 3678 2335 87 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4094 5304 4094 Size: 1210 4992 BclI T'GATC_A Cuts at: 1548 2112 1548 Size: 564 5638 BfrBI ATG'CAT Cuts at: 2590 2590 Size: 6202 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4813 136 Size: 107 122 71 631 605 3141 1525 Fragments arranged by size: 3141 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6197 6197 Size: 6202 BlpI GC'TnA_GC Cuts at: 2847 3619 2847 Size: 772 5430 Bme1580I G_kGCm'C Cuts at: 1790 2058 2319 4263 5419 1790 Size: 268 261 1944 1156 2573 Fragments arranged by size: 2573 1944 1156 268 261 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2155 3067 3933 Size: 416 53 83 399 24 738 912 866 Cuts at: 3933 4891 442 Size: 958 1753 Fragments arranged by size: 1753 958 912 866 738 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6202 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2930 4862 1239 Size: 1691 1932 2579 Fragments arranged by size: 2579 1932 1691 Bpu10I CC'TnA_GC Cuts at: 2155 2479 2155 Size: 324 5878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4327 5192 5490 4327 Size: 865 298 5039 Fragments arranged by size: 5039 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2016 2132 2165 2948 4865 882 Size: 617 517 116 33 783 1917 2219 Fragments arranged by size: 2219 1917 783 617 517 116 33 BsaAI yAC'GTr Cuts at: 493 1204 3993 493 Size: 711 2789 2702 Fragments arranged by size: 2789 2702 711 BsaBI GATnn'nnATC Cuts at: 2117 2525 6196 2117 Size: 408 3671 2123 Fragments arranged by size: 3671 2123 408 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4009 4391 5026 275 Size: 53 83 186 936 2476 382 635 1451 Fragments arranged by size: 2476 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2018 2892 3217 4635 5033 5307 5454 5865 2018 Size: 874 325 1418 398 274 147 411 2355 Fragments arranged by size: 2355 1418 874 411 398 325 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3129 3159 1178 Size: 30 165 30 1726 30 4221 Fragments arranged by size: 4221 1726 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6202 BseYI C'CCAG_C Cuts at: 2026 2142 2356 2738 3080 5405 5901 2026 Size: 116 214 382 342 2325 496 2327 Fragments arranged by size: 2327 2325 496 382 342 214 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5629 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2242 3652 4413 5441 1540 Size: 275 186 241 1410 761 1028 2301 Fragments arranged by size: 2301 1410 1028 761 275 241 186 BsiHKAI G_wGCw'C Cuts at: 2480 4263 4348 5419 2480 Size: 1783 85 1071 3263 Fragments arranged by size: 3263 1783 1071 85 BsmI GAATG_Cn' Cuts at: 3713 3806 3713 Size: 93 6109 BsmBI CGTCTCn'nnnn_ Cuts at: 3118 3118 Size: 6202 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2319 2480 3521 4263 4348 5419 Size: 399 268 261 161 1041 742 85 1071 Cuts at: 5419 1391 Size: 2174 Fragments arranged by size: 2174 1071 1041 742 399 268 261 161 85 BspEI T'CCGG_A Cuts at: 5865 5865 Size: 6202 BspHI T'CATG_A Cuts at: 2766 4091 5821 2766 Size: 1325 1730 3147 Fragments arranged by size: 3147 1730 1325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3185 3557 844 Size: 2341 372 3489 Fragments arranged by size: 3489 2341 372 BsrBI CCG'CTC Cuts at: 1394 1823 4089 1394 Size: 429 2266 3507 Fragments arranged by size: 3507 2266 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2555 4697 4871 65 Size: 1183 1307 2142 174 1396 Fragments arranged by size: 2142 1396 1307 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2747 3217 4846 5033 1700 Size: 110 937 470 1629 187 2869 Fragments arranged by size: 2869 1629 937 470 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6202 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6202 BssSI C'ACGA_G Cuts at: 2090 4262 5274 2090 Size: 2172 1012 3018 Fragments arranged by size: 3018 2172 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6202 BstBI TT'CG_AA Cuts at: 5006 5006 Size: 6202 BstEII G'GTnAC_C Cuts at: 1184 2602 1184 Size: 1418 4784 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2872 2011 Size: 861 5341 BstYI r'GATC_y Cuts at: 1972 3315 3384 3532 3899 4284 4301 5753 5839 Size: 1343 69 148 367 385 17 1452 86 Cuts at: 5839 5851 6197 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1343 385 367 346 148 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5650 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2384 487 Size: 683 56 1158 4305 Fragments arranged by size: 4305 1158 683 56 BtsI GCAGTG_nn' Cuts at: 3718 4512 4532 3718 Size: 794 20 5388 Fragments arranged by size: 5388 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6167 DraI TTT'AAA Cuts at: 3506 3506 Size: 6202 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5209 809 Size: 507 3893 1802 Fragments arranged by size: 3893 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2787 2874 Size: 54 1425 50 275 18 183 774 87 Cuts at: 2874 3649 4538 6167 8 Size: 775 889 1629 43 Fragments arranged by size: 1629 1425 889 775 774 275 183 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3649 1537 Size: 275 1837 4090 Fragments arranged by size: 4090 1837 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 3014 4132 1353 Size: 1053 608 1118 3423 Fragments arranged by size: 3423 1118 1053 608 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4789 5163 5309 387 Size: 1064 138 3200 374 146 1280 Fragments arranged by size: 3200 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2478 2478 Size: 6202 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2355 2930 4265 4862 5649 5917 1239 Size: 393 723 575 1335 597 787 268 1524 Fragments arranged by size: 1524 1335 787 723 597 575 393 268 EcoO109I rG'GnC_Cy Cuts at: 2185 2483 2185 Size: 298 5904 EcoRI G'AATT_C Cuts at: 2711 3511 3889 2711 Size: 800 378 5024 Fragments arranged by size: 5024 800 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2471 2471 Size: 6202 FspI TGC'GCA Cuts at: 4708 4708 Size: 6202 HaeII r_GCGC'y Cuts at: 1678 5349 1678 Size: 3671 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 2941 2973 3053 Size: 32 135 32 743 32 594 32 80 Cuts at: 3053 3085 3292 3324 4843 4875 4917 4949 1373 Size: 32 207 32 1519 32 42 32 2626 Fragments arranged by size: 2626 1519 743 594 207 135 80 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3492 3546 3792 669 Size: 988 281 1266 288 54 246 3079 Fragments arranged by size: 3079 1266 988 288 281 246 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 6202 HpaI GTT'AAC Cuts at: 3204 3492 3792 3204 Size: 288 300 5614 Fragments arranged by size: 5614 300 288 KpnI G_GTAC'C Cuts at: 2641 3527 2641 Size: 886 5316 MfeI C'AATT_G Cuts at: 2532 3801 2532 Size: 1269 4933 MluI A'CGCG_T Cuts at: 6078 6078 Size: 6202 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2646 3173 5316 5500 1305 Size: 1341 527 2143 184 2007 Fragments arranged by size: 2143 2007 1341 527 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2015 2789 2876 10 Size: 54 1425 343 183 774 87 3336 Fragments arranged by size: 3336 1425 774 343 183 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2595 4160 4519 4678 518 Size: 648 843 586 1565 359 159 2042 Fragments arranged by size: 2042 1565 843 648 586 359 159 MspA1I CmG'CkG Cuts at: 1606 3618 4295 5443 5688 1606 Size: 2012 677 1148 245 2120 Fragments arranged by size: 2120 2012 1148 677 245 NaeI GCC'GGC Cuts at: 2749 2749 Size: 6202 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5650 NdeI CA'TA_TG Cuts at: 387 387 Size: 6202 NgoMIV G'CCGG_C Cuts at: 2747 2747 Size: 6202 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6202 NotI GC'GGCC_GC Cuts at: 3649 3649 Size: 6202 NsiI A_TGCA'T Cuts at: 2592 2592 Size: 6202 NspI r_CATG'y Cuts at: 1132 1229 2590 3560 5105 1132 Size: 97 1361 970 1545 2229 Fragments arranged by size: 2229 1545 1361 970 97 PasI CC'CwG_GG Cuts at: 1348 2051 2812 1348 Size: 703 761 4738 Fragments arranged by size: 4738 761 703 PciI A'CATG_T Cuts at: 5101 5101 Size: 6202 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6202 PfoI T'CCnGG_A Cuts at: 2568 3387 2568 Size: 819 5383 PmeI GTTT'AAAC Cuts at: 3506 3506 Size: 6202 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3059 3091 3129 3161 4271 4303 5784 5816 3059 Size: 32 38 32 1110 32 1481 32 3445 Fragments arranged by size: 3445 1481 1110 38 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2185 2185 Size: 6202 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6202 PsiI TTA'TAA Cuts at: 3772 3772 Size: 6202 PstI C_TGCA'G Cuts at: 830 3554 830 Size: 2724 3478 PvuI CG_AT'CG Cuts at: 2001 2242 2001 Size: 241 5961 SacI G_AGCT'C Cuts at: 2480 2480 Size: 6202 SalI G'TCGA_C Cuts at: 1936 3544 1936 Size: 1608 4594 SanDI GG'GwC_CC Cuts at: 2185 2185 Size: 6202 SapI GCTCTTCn'nnn_ Cuts at: 2406 2406 Size: 6202 SbfI CC_TGCA'GG Cuts at: 3554 3554 Size: 6202 ScaI AGT'ACT Cuts at: 2231 4450 6162 2231 Size: 2219 1712 2271 Fragments arranged by size: 2271 2219 1712 SfcI C'TryA_G Cuts at: 826 1045 3126 3550 4685 5366 5557 826 Size: 219 2081 424 1135 681 191 1471 Fragments arranged by size: 2081 1471 1135 681 424 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6202 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6202 SmaI CCC'GGG Cuts at: 1872 3529 1872 Size: 1657 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4306 5207 5469 820 Size: 197 926 2363 901 262 1553 Fragments arranged by size: 2363 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3993 493 Size: 3500 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6202 SphI G_CATG'C Cuts at: 1132 3560 1132 Size: 2428 3774 SspI AAT'ATT Cuts at: 5 52 3478 4126 5 Size: 47 3426 648 2081 Fragments arranged by size: 3426 2081 648 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6202 StyI C'CwwG_G Cuts at: 513 1065 3453 3641 513 Size: 552 2388 188 3074 Fragments arranged by size: 3074 2388 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2073 4229 4246 4399 4584 5029 325 Size: 1255 493 2156 17 153 185 445 1498 Fragments arranged by size: 2156 1498 1255 493 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2229 2271 4448 Size: 274 80 33 51 1443 252 42 2177 Cuts at: 4448 6160 96 Size: 1712 138 Fragments arranged by size: 2177 1712 1443 274 252 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2603 2635 2862 2894 3156 3188 6162 6194 2603 Size: 32 227 32 262 32 2974 32 2611 Fragments arranged by size: 2974 2611 262 227 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6202 XbaI T'CTAG_A Cuts at: 3538 3538 Size: 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3096 3451 3096 Size: 355 5847 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6202 XmaI C'CCGG_G Cuts at: 1870 3527 1870 Size: 1657 4545 XmnI GAAnn'nnTTC Cuts at: 2340 4331 2340 Size: 1991 4211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4010 276 Size: 53 83 186 936 2476 2468 Fragments arranged by size: 2476 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI