(Circular) (Six-base) MAPSORT of: pFN21AB9872.seq Check: 9464 from: 1 to: 6190 pFN21AB9872 6190 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 4253 5637 5905 1632 Size: 968 1653 1384 268 1917 Fragments arranged by size: 1917 1653 1384 968 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 2337 3135 5089 6066 1203 Size: 1134 798 1954 977 1327 Fragments arranged by size: 1954 1327 1134 977 798 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6190 AlwNI CAG_nnn'CTG Cuts at: 2947 3933 5505 2947 Size: 986 1572 3632 Fragments arranged by size: 3632 1572 986 ApaI G_GGCC'C Cuts at: 2499 2499 Size: 6190 ApaLI G'TGCA_C Cuts at: 4247 5403 4247 Size: 1156 5034 ApoI r'AATT_y Cuts at: 1493 3499 3725 3877 5986 1493 Size: 2006 226 152 2109 1697 Fragments arranged by size: 2109 2006 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 2050 2438 2483 2491 2573 3515 1870 Size: 73 107 388 45 8 82 942 4545 Fragments arranged by size: 4545 942 388 107 82 73 45 8 BamHI G'GATC_C Cuts at: 3520 3887 3520 Size: 367 5823 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2603 2813 3511 4966 618 Size: 325 217 627 816 210 698 1455 1842 Fragments arranged by size: 1842 1455 816 698 627 325 217 210 BanII G_rGCy'C Cuts at: 1391 2492 2499 3173 3509 1391 Size: 1101 7 674 336 4072 Fragments arranged by size: 4072 1101 674 336 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4381 4415 1903 Size: 34 2444 34 3678 Fragments arranged by size: 3678 2444 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3089 3119 4082 5292 3089 Size: 30 963 1210 3987 Fragments arranged by size: 3987 1210 963 30 BclI T'GATC_A Cuts at: 1548 2124 1548 Size: 576 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4801 136 Size: 107 122 71 631 605 3129 1525 Fragments arranged by size: 3129 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 2826 3607 2826 Size: 781 5409 Bme1580I G_kGCm'C Cuts at: 1790 2331 2499 2606 2816 4251 5407 1790 Size: 541 168 107 210 1435 1156 2573 Fragments arranged by size: 2573 1435 1156 541 210 168 107 BmgBI CAC'GTC Cuts at: 1318 3292 1318 Size: 1974 4216 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3921 4879 442 Size: 416 53 83 399 24 2504 958 1753 Fragments arranged by size: 2504 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2582 2924 3058 4850 1239 Size: 1343 342 134 1792 2579 Fragments arranged by size: 2579 1792 1343 342 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 4315 5180 5478 2144 Size: 2171 865 298 2856 Fragments arranged by size: 2856 2171 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4853 882 Size: 617 3354 2219 Fragments arranged by size: 3354 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3981 493 Size: 711 2777 2702 Fragments arranged by size: 2777 2702 711 BsaBI GATnn'nnATC Cuts at: 2129 6184 2129 Size: 4055 2135 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2274 3997 4379 5014 Size: 53 83 186 936 741 1723 382 635 Cuts at: 5014 275 Size: 1451 Fragments arranged by size: 1723 1451 936 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4623 5021 5295 5442 5853 4623 Size: 398 274 147 411 4960 Fragments arranged by size: 4960 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2327 2342 2357 2372 1178 Size: 30 165 30 924 15 15 15 4996 Fragments arranged by size: 4996 924 165 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2390 2435 2438 1186 Size: 1177 27 45 3 4938 Fragments arranged by size: 4938 1177 45 27 3 BseYI C'CCAG_C Cuts at: 2191 2264 2735 2809 5393 5889 2191 Size: 73 471 74 2584 496 2492 Fragments arranged by size: 2584 2492 496 471 74 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3051 1163 Size: 573 1315 4302 Fragments arranged by size: 4302 1315 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 2254 2305 3640 4401 5429 Size: 275 186 45 208 51 1335 761 1028 Cuts at: 5429 1540 Size: 2301 Fragments arranged by size: 2301 1335 1028 761 275 208 186 51 45 BsiHKAI G_wGCw'C Cuts at: 2492 4251 4336 5407 2492 Size: 1759 85 1071 3275 Fragments arranged by size: 3275 1759 1071 85 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 BsmBI CGTCTCn'nnnn_ Cuts at: 2144 2524 2558 2144 Size: 380 34 5776 Fragments arranged by size: 5776 380 34 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2492 2499 2606 2816 3173 3509 Size: 399 541 161 7 107 210 357 336 Cuts at: 3509 4251 4336 5407 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 541 399 357 336 210 161 107 85 7 BspEI T'CCGG_A Cuts at: 5853 5853 Size: 6190 BspHI T'CATG_A Cuts at: 2763 4079 5809 2763 Size: 1316 1730 3144 Fragments arranged by size: 3144 1730 1316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3545 844 Size: 2701 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4077 1394 Size: 429 2254 3507 Fragments arranged by size: 3507 2254 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4685 4859 65 Size: 1183 3437 174 1396 Fragments arranged by size: 3437 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2259 2317 4834 5021 1700 Size: 110 449 58 2517 187 2869 Fragments arranged by size: 2869 2517 449 187 110 58 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6190 BssSI C'ACGA_G Cuts at: 2102 4250 5262 2102 Size: 2148 1012 3030 Fragments arranged by size: 3030 2148 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6190 BstBI TT'CG_AA Cuts at: 4994 4994 Size: 6190 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstXI CCAn_nnnn'nTGG Cuts at: 2866 2866 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2943 3520 3887 4272 4289 5741 5827 5839 Size: 971 577 367 385 17 1452 86 12 Cuts at: 5839 6185 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 971 577 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2231 2876 2231 Size: 645 5545 BtgI C'CryG_G Cuts at: 513 1065 2382 2628 513 Size: 552 1317 246 4075 Fragments arranged by size: 4075 1317 552 246 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2494 3169 487 Size: 683 56 1268 675 3508 Fragments arranged by size: 3508 1268 683 675 56 BtsI GCAGTG_nn' Cuts at: 3039 3441 3706 4500 4520 3039 Size: 402 265 794 20 4709 Fragments arranged by size: 4709 794 402 265 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 3494 3494 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2014 5197 809 Size: 507 698 3183 1802 Fragments arranged by size: 3183 1802 698 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2302 2784 Size: 54 1425 50 275 18 213 259 482 Cuts at: 2784 2868 3637 4526 6155 8 Size: 84 769 889 1629 43 Fragments arranged by size: 1629 1425 889 769 482 275 259 213 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2043 2302 3637 1537 Size: 275 231 259 1335 4090 Fragments arranged by size: 4090 1335 275 259 231 EarI CTCTTCn'nnn_ Cuts at: 1353 2477 3008 3264 3372 4120 1353 Size: 1124 531 256 108 748 3423 Fragments arranged by size: 3423 1124 748 531 256 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3065 4777 5151 5297 387 Size: 1064 138 1476 1712 374 146 1280 Fragments arranged by size: 1712 1476 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2490 2490 Size: 6190 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2582 2600 2924 3058 4253 4850 5637 Size: 393 950 18 324 134 1195 597 787 Cuts at: 5637 5905 1239 Size: 268 1524 Fragments arranged by size: 1524 1195 950 787 597 393 324 268 134 18 EcoO109I rG'GnC_Cy Cuts at: 2495 2935 2495 Size: 440 5750 EcoRI G'AATT_C Cuts at: 3499 3877 3499 Size: 378 5812 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2764 2764 Size: 6190 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 2431 2458 5337 1678 Size: 753 27 2879 2531 Fragments arranged by size: 2879 2531 753 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2327 2359 3147 3179 4831 Size: 32 135 32 755 32 788 32 1652 Cuts at: 4831 4863 4905 4937 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1652 788 755 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2323 3534 3780 669 Size: 988 281 385 1211 246 3079 Fragments arranged by size: 3079 1211 988 385 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 3150 3201 3433 3789 3150 Size: 51 232 356 5551 Fragments arranged by size: 5551 356 232 51 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2670 5304 5488 1305 Size: 1365 2634 184 2007 Fragments arranged by size: 2634 2007 1365 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 2870 10 Size: 54 1425 343 954 84 3330 Fragments arranged by size: 3330 1425 954 343 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4148 4507 4666 518 Size: 648 843 2139 359 159 2042 Fragments arranged by size: 2139 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3606 4283 5431 5676 1606 Size: 2000 677 1148 245 2120 Fragments arranged by size: 2120 2000 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2382 513 Size: 552 1317 4321 Fragments arranged by size: 4321 1317 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 2302 3637 2302 Size: 1335 4855 NspI r_CATG'y Cuts at: 1132 1229 2215 2341 3139 3548 5093 1132 Size: 97 986 126 798 409 1545 2229 Fragments arranged by size: 2229 1545 986 798 409 126 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 2337 3135 5089 2337 Size: 798 1954 3438 Fragments arranged by size: 3438 1954 798 PflMI CCAn_nnn'nTGG Cuts at: 1108 2947 1108 Size: 1839 4351 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 6190 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3327 1613 Size: 1714 4476 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PspOMI G'GGCC_C Cuts at: 2495 2495 Size: 6190 PstI C_TGCA'G Cuts at: 830 3542 830 Size: 2712 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 SacI G_AGCT'C Cuts at: 2492 2492 Size: 6190 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 2477 3264 2477 Size: 787 5403 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 2243 4438 6150 2243 Size: 2195 1712 2283 Fragments arranged by size: 2283 2195 1712 SfcI C'TryA_G Cuts at: 826 1045 3120 3538 4673 5354 5545 826 Size: 219 2075 418 1135 681 191 1471 Fragments arranged by size: 2075 1471 1135 681 418 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 2052 3517 1872 Size: 180 1465 4545 Fragments arranged by size: 4545 1465 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 4294 5195 5457 820 Size: 197 926 216 2135 901 262 1553 Fragments arranged by size: 2135 1553 926 901 262 216 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 2215 3548 1132 Size: 1083 1333 3774 Fragments arranged by size: 3774 1333 1083 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 2382 3629 513 Size: 552 1317 1247 3074 Fragments arranged by size: 3074 1317 1247 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3497 4217 4234 4387 4572 5017 325 Size: 1255 1917 720 17 153 185 445 1498 Fragments arranged by size: 1917 1498 1255 720 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2241 3036 3363 Size: 274 80 33 51 1443 264 795 327 Cuts at: 3363 4436 6148 96 Size: 1073 1712 138 Fragments arranged by size: 1712 1443 1073 795 327 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2669 2701 3281 3313 6150 6182 2669 Size: 32 580 32 2837 32 2677 Fragments arranged by size: 2837 2677 580 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2063 2188 2063 Size: 125 6065 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 2050 3515 1870 Size: 180 1465 4545 Fragments arranged by size: 4545 1465 180 XmnI GAAnn'nnTTC Cuts at: 4319 4319 Size: 6190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI