(Circular) (Six-base) MAPSORT of: pFN21AB9871.seq Check: 4180 from: 1 to: 6175 pFN21AB9871 6175 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2823 3005 3141 3231 2823 Size: 182 136 90 5767 Fragments arranged by size: 5767 182 136 90 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2776 4238 5622 5890 1632 Size: 1144 1462 1384 268 1917 Fragments arranged by size: 1917 1462 1384 1144 268 AfeI AGC'GCT Cuts at: 2929 3191 3248 3275 2929 Size: 262 57 27 5829 Fragments arranged by size: 5829 262 57 27 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 2507 1166 Size: 1341 4834 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3083 1184 Size: 1899 4276 AlwNI CAG_nnn'CTG Cuts at: 3918 5490 3918 Size: 1572 4603 ApaI G_GGCC'C Cuts at: 3038 3038 Size: 6175 ApaLI G'TGCA_C Cuts at: 2498 4232 5388 2498 Size: 1734 1156 3285 Fragments arranged by size: 3285 1734 1156 ApoI r'AATT_y Cuts at: 1493 3484 3710 3862 5971 1493 Size: 1991 226 152 2109 1697 Fragments arranged by size: 2109 1991 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2584 3085 3352 3446 3452 3500 1870 Size: 73 641 501 267 94 6 48 4545 Fragments arranged by size: 4545 641 501 267 94 73 48 6 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2621 2801 2941 3219 3456 Size: 325 217 627 834 180 140 278 237 Cuts at: 3456 3496 4951 618 Size: 40 1455 1842 Fragments arranged by size: 1842 1455 834 627 325 278 237 217 180 140 40 BanII G_rGCy'C Cuts at: 1391 2615 2724 2918 2957 3038 3353 3453 3494 Size: 1224 109 194 39 81 315 100 41 Cuts at: 3494 1391 Size: 4072 Fragments arranged by size: 4072 1224 315 194 109 100 81 41 39 BbeI G_GCGC'C Cuts at: 2625 2805 2945 2625 Size: 180 140 5855 Fragments arranged by size: 5855 180 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3317 928 Size: 971 1418 3786 Fragments arranged by size: 3786 1418 971 BbvCI CC'TCA_GC Cuts at: 2968 3296 2968 Size: 328 5847 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4366 4400 1903 Size: 34 50 34 2345 34 3678 Fragments arranged by size: 3678 2345 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6170 6170 Size: 6175 BlpI GC'TnA_GC Cuts at: 2819 3592 2819 Size: 773 5402 Bme1580I G_kGCm'C Cuts at: 1790 2502 3038 3459 4236 5392 1790 Size: 712 536 421 777 1156 2573 Fragments arranged by size: 2573 1156 777 712 536 421 BmgBI CAC'GTC Cuts at: 1318 2641 2662 1318 Size: 1323 21 4831 Fragments arranged by size: 4831 1323 21 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3906 4864 442 Size: 416 53 83 399 24 2489 958 1753 Fragments arranged by size: 2489 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2629 2629 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2167 2272 2293 2338 2611 4835 1239 Size: 928 105 21 45 273 2224 2579 Fragments arranged by size: 2579 2224 928 273 105 45 21 Bpu10I CC'TnA_GC Cuts at: 2968 3296 2968 Size: 328 5847 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2143 3107 4300 5165 5463 2143 Size: 964 1193 865 298 2855 Fragments arranged by size: 2855 1193 964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3307 3966 493 Size: 711 2103 659 2702 Fragments arranged by size: 2702 2103 711 659 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2622 2802 2942 3982 Size: 53 83 186 936 1089 180 140 1040 Cuts at: 3982 4364 4999 275 Size: 382 635 1451 Fragments arranged by size: 1451 1089 1040 936 635 382 186 180 140 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5950 Fragments arranged by size: 5950 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2044 2101 2437 2797 2956 1186 Size: 858 57 336 360 159 4405 Fragments arranged by size: 4405 858 360 336 159 57 BseYI C'CCAG_C Cuts at: 2039 2930 2947 3031 5378 5874 2039 Size: 891 17 84 2347 496 2340 Fragments arranged by size: 2347 2340 891 496 84 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 2687 2720 3053 1163 Size: 573 948 3 33 333 4285 Fragments arranged by size: 4285 948 573 333 33 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2220 2619 2989 3625 4386 5414 Size: 275 186 219 399 370 636 761 1028 Cuts at: 5414 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 636 399 370 275 219 186 BsiHKAI G_wGCw'C Cuts at: 2502 3396 4236 4321 5392 2502 Size: 894 840 85 1071 3285 Fragments arranged by size: 3285 1071 894 840 85 BsiWI C'GTAC_G Cuts at: 2556 2556 Size: 6175 BsmI GAATG_Cn' Cuts at: 3686 3779 3686 Size: 93 6082 BsmBI CGTCTCn'nnnn_ Cuts at: 2052 2052 Size: 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2502 2615 2724 2918 2957 3038 3353 Size: 399 712 113 109 194 39 81 315 Cuts at: 3353 3396 3453 3459 3494 4236 4321 5392 1391 Size: 43 57 6 35 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 712 399 315 194 113 109 85 81 57 43 39 35 6 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 3005 3141 3231 3530 844 Size: 1979 182 136 90 299 3489 Fragments arranged by size: 3489 1979 299 182 136 90 BsrBI CCG'CTC Cuts at: 1394 1823 2746 3028 3154 4062 1394 Size: 429 923 282 126 908 3507 Fragments arranged by size: 3507 923 908 429 282 126 BsrDI GCAATG_nn' Cuts at: 65 1248 4670 4844 65 Size: 1183 3422 174 1396 Fragments arranged by size: 3422 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2479 2618 2648 2903 3236 4819 5006 Size: 110 669 139 30 255 333 1583 187 Cuts at: 5006 1700 Size: 2869 Fragments arranged by size: 2869 1583 669 333 255 187 139 110 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstYI r'GATC_y Cuts at: 1972 3505 3872 4257 4274 5726 5812 5824 6170 Size: 1533 367 385 17 1452 86 12 346 Cuts at: 6170 1972 Size: 1977 Fragments arranged by size: 1977 1533 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2220 2232 2241 2534 3402 513 Size: 552 1155 12 9 293 868 3286 Fragments arranged by size: 3286 1155 868 552 293 12 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 3691 4485 4505 3691 Size: 794 20 5361 Fragments arranged by size: 5361 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2616 2901 Size: 54 1425 50 275 18 387 399 285 Cuts at: 2901 2986 3622 4511 6140 8 Size: 85 636 889 1629 43 Fragments arranged by size: 1629 1425 889 636 399 387 285 275 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2217 2616 2986 3622 1537 Size: 275 405 399 370 636 4090 Fragments arranged by size: 4090 636 405 399 370 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2077 2125 4105 1353 Size: 724 48 1980 3423 Fragments arranged by size: 3423 1980 724 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2116 4762 5136 5282 387 Size: 1064 138 527 2646 374 146 1280 Fragments arranged by size: 2646 1280 1064 527 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2272 2293 2338 2611 2776 4238 Size: 393 535 105 21 45 273 165 1462 Cuts at: 4238 4835 5622 5890 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1462 787 597 535 393 273 268 165 105 45 21 EcoO109I rG'GnC_Cy Cuts at: 2178 2392 3035 3064 2178 Size: 214 643 29 5289 Fragments arranged by size: 5289 643 214 29 EcoRI G'AATT_C Cuts at: 3484 3862 3484 Size: 378 5797 FspI TGC'GCA Cuts at: 3284 4681 3284 Size: 1397 4778 HaeII r_GCGC'y Cuts at: 1678 2295 2319 2625 2805 2931 2945 3193 3250 Size: 617 24 306 180 126 14 248 57 Cuts at: 3250 3277 3402 5322 1678 Size: 27 125 1920 2531 Fragments arranged by size: 2531 1920 617 306 248 180 126 125 57 27 24 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4816 4848 4890 4922 1373 Size: 32 135 32 3244 32 42 32 2626 Fragments arranged by size: 3244 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3765 669 Size: 988 281 1581 246 3079 Fragments arranged by size: 3079 1581 988 281 246 HindIII A'AGCT_T Cuts at: 748 2161 748 Size: 1413 4762 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KasI G'GCGC_C Cuts at: 2621 2801 2941 2621 Size: 180 140 5855 Fragments arranged by size: 5855 180 140 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 3228 5289 5473 1305 Size: 975 948 2061 184 2007 Fragments arranged by size: 2061 2007 975 948 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2507 4133 4492 4651 518 Size: 648 1341 1626 359 159 2042 Fragments arranged by size: 2042 1626 1341 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2133 2222 2234 2243 2268 2415 2536 2631 Size: 527 89 12 9 25 147 121 95 Cuts at: 2631 2799 2899 2947 3075 3217 3360 3404 3591 Size: 168 100 48 128 142 143 44 187 Cuts at: 3591 4268 5416 5661 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 527 245 187 168 147 143 142 128 121 100 95 89 48 44 25 12 9 NaeI GCC'GGC Cuts at: 2620 2650 2905 2620 Size: 30 255 5890 Fragments arranged by size: 5890 255 30 NarI GG'CG_CC Cuts at: 2622 2802 2942 2622 Size: 180 140 5855 Fragments arranged by size: 5855 180 140 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5623 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NgoMIV G'CCGG_C Cuts at: 2618 2648 2903 2618 Size: 30 255 5890 Fragments arranged by size: 5890 255 30 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 2217 3622 2217 Size: 1405 4770 NspI r_CATG'y Cuts at: 1132 1229 3533 5078 1132 Size: 97 2304 1545 2229 Fragments arranged by size: 2304 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 2634 1108 Size: 1526 4649 PfoI T'CCnGG_A Cuts at: 2183 2183 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PmlI CAC'GTG Cuts at: 3307 3307 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2180 2212 4244 4276 5757 5789 2180 Size: 32 2032 32 1481 32 2566 Fragments arranged by size: 2566 2032 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PspOMI G'GGCC_C Cuts at: 3034 3034 Size: 6175 PstI C_TGCA'G Cuts at: 830 2523 3216 3441 3527 830 Size: 1693 693 225 86 3478 Fragments arranged by size: 3478 1693 693 225 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2133 2268 2631 3075 2133 Size: 135 363 444 5233 Fragments arranged by size: 5233 444 363 135 SacII CC_GC'GG Cuts at: 2223 2235 2244 2537 3405 2223 Size: 12 9 293 868 4993 Fragments arranged by size: 4993 868 293 12 9 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SapI GCTCTTCn'nnn_ Cuts at: 2077 2077 Size: 6175 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2519 3212 3437 3523 4658 5339 5530 Size: 219 1474 693 225 86 1135 681 191 Cuts at: 5530 826 Size: 1471 Fragments arranged by size: 1474 1471 1135 693 681 225 219 191 86 SfoI GGC'GCC Cuts at: 2623 2803 2943 2623 Size: 180 140 5855 Fragments arranged by size: 5855 180 140 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 3236 1700 Size: 1536 4639 SmaI CCC'GGG Cuts at: 1872 2586 3087 3454 3502 1872 Size: 714 501 367 48 4545 Fragments arranged by size: 4545 714 501 367 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 2158 3122 4279 5180 5442 820 Size: 197 926 215 964 1157 901 262 1553 Fragments arranged by size: 1553 1157 964 926 901 262 215 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SrfI GCCC'GGGC Cuts at: 3454 3454 Size: 6175 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 2775 1517 Size: 1258 4917 StyI C'CwwG_G Cuts at: 513 1065 2493 3614 513 Size: 552 1428 1121 3074 Fragments arranged by size: 3074 1428 1121 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2582 4202 4219 4372 4557 5002 325 Size: 1255 1002 1620 17 153 185 445 1498 Fragments arranged by size: 1620 1498 1255 1002 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4421 6133 96 Size: 274 80 33 51 1443 2444 1712 138 Fragments arranged by size: 2444 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6135 6167 6135 Size: 32 6143 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 2584 3085 3452 3500 1870 Size: 714 501 367 48 4545 Fragments arranged by size: 4545 714 501 367 48 XmnI GAAnn'nnTTC Cuts at: 3248 4304 3248 Size: 1056 5119 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SwaI XcmI