(Circular) (Six-base) MAPSORT of: pFN21AB9869.seq Check: 687 from: 1 to: 6085 pFN21AB9869 6085 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3072 3895 278 Size: 53 83 186 936 1536 823 2468 Fragments arranged by size: 2468 1536 936 823 186 83 53 AccI GT'mk_AC Cuts at: 1937 2646 3428 1937 Size: 709 782 4594 Fragments arranged by size: 4594 782 709 Acc65I G'GTAC_C Cuts at: 3406 3406 Size: 6085 AclI AA'CG_TT Cuts at: 1563 4212 4585 1563 Size: 2649 373 3063 Fragments arranged by size: 3063 2649 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2061 2096 4148 5532 5800 1632 Size: 429 35 2052 1384 268 1917 Fragments arranged by size: 2052 1917 1384 429 268 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5888 AflIII A'CryG_T Cuts at: 1203 4984 5961 1203 Size: 3781 977 1327 Fragments arranged by size: 3781 1327 977 AgeI A'CCGG_T Cuts at: 4916 4916 Size: 6085 AhdI GACnn_n'nnGTC Cuts at: 4814 4814 Size: 6085 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6085 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2479 2846 1184 Size: 1295 367 4423 Fragments arranged by size: 4423 1295 367 AlwNI CAG_nnn'CTG Cuts at: 2086 2498 2966 3828 5400 2086 Size: 412 468 862 1572 2771 Fragments arranged by size: 2771 1572 862 468 412 ApaI G_GGCC'C Cuts at: 2810 2810 Size: 6085 ApaLI G'TGCA_C Cuts at: 2225 4142 5298 2225 Size: 1917 1156 3012 Fragments arranged by size: 3012 1917 1156 ApoI r'AATT_y Cuts at: 1493 2068 3394 3620 3772 5881 1493 Size: 575 1326 226 152 2109 1697 Fragments arranged by size: 2109 1697 1326 575 226 152 AseI AT'TA_AT Cuts at: 160 1032 4639 160 Size: 872 3607 1606 Fragments arranged by size: 3607 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2172 2213 3410 1870 Size: 73 70 159 41 1197 4545 Fragments arranged by size: 4545 1197 159 73 70 41 BamHI G'GATC_C Cuts at: 3415 3782 3415 Size: 367 5718 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3406 4861 618 Size: 325 217 627 1619 1455 1842 Fragments arranged by size: 1842 1619 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2283 2810 2999 3404 1391 Size: 892 527 189 405 4072 Fragments arranged by size: 4072 892 527 405 189 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5114 BbvCI CC'TCA_GC Cuts at: 2959 2959 Size: 6085 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4276 4310 1903 Size: 34 2339 34 3678 Fragments arranged by size: 3678 2339 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3977 5187 3977 Size: 1210 4875 BclI T'GATC_A Cuts at: 1548 1548 Size: 6085 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2128 2331 2423 Size: 107 122 71 631 605 456 203 92 Cuts at: 2423 4696 136 Size: 2273 1525 Fragments arranged by size: 2273 1525 631 605 456 203 122 107 92 71 BglII A'GATC_T Cuts at: 6080 6080 Size: 6085 BlpI GC'TnA_GC Cuts at: 2106 3502 2106 Size: 1396 4689 Bme1580I G_kGCm'C Cuts at: 1790 2229 2556 2810 3203 4146 5302 1790 Size: 439 327 254 393 943 1156 2573 Fragments arranged by size: 2573 1156 943 439 393 327 254 BmgBI CAC'GTC Cuts at: 1318 2350 1318 Size: 1032 5053 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3254 3816 4774 Size: 416 53 83 399 24 1837 562 958 Cuts at: 4774 442 Size: 1753 Fragments arranged by size: 1837 1753 958 562 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6085 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3263 4745 1239 Size: 2024 1482 2579 Fragments arranged by size: 2579 2024 1482 Bpu10I CC'TnA_GC Cuts at: 2959 2959 Size: 6085 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3282 4210 5075 5373 3282 Size: 928 865 298 3994 Fragments arranged by size: 3994 928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4748 882 Size: 617 3249 2219 Fragments arranged by size: 3249 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3876 493 Size: 711 2672 2702 Fragments arranged by size: 2702 2672 711 BsaBI GATnn'nnATC Cuts at: 6079 6079 Size: 6085 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2090 3069 3892 4274 Size: 53 83 186 936 557 979 823 382 Cuts at: 4274 4909 275 Size: 635 1451 Fragments arranged by size: 1451 979 936 823 635 557 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2137 4518 4916 5190 5337 5748 2137 Size: 2381 398 274 147 411 2474 Fragments arranged by size: 2474 2381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5860 Fragments arranged by size: 5860 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2072 2947 3017 1186 Size: 847 39 875 70 4254 Fragments arranged by size: 4254 875 847 70 39 BseYI C'CCAG_C Cuts at: 2218 2698 5288 5784 2218 Size: 480 2590 496 2519 Fragments arranged by size: 2590 2519 496 480 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2916 1163 Size: 573 1180 4332 Fragments arranged by size: 4332 1180 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2316 2427 3535 4296 5324 1540 Size: 275 186 315 111 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 315 275 186 111 BsiHKAI G_wGCw'C Cuts at: 2229 2739 4146 4231 5302 2229 Size: 510 1407 85 1071 3012 Fragments arranged by size: 3012 1407 1071 510 85 BsmI GAATG_Cn' Cuts at: 2468 3050 3596 3689 2468 Size: 582 546 93 4864 Fragments arranged by size: 4864 582 546 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2283 2556 2739 2810 2999 3203 Size: 399 439 54 273 183 71 189 204 Cuts at: 3203 3404 4146 4231 5302 1391 Size: 201 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 273 204 201 189 183 85 71 54 BspEI T'CCGG_A Cuts at: 5748 5748 Size: 6085 BspHI T'CATG_A Cuts at: 3974 5704 3974 Size: 1730 4355 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3440 844 Size: 2596 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2165 2862 2979 3972 1394 Size: 429 342 697 117 993 3507 Fragments arranged by size: 3507 993 697 429 342 117 BsrDI GCAATG_nn' Cuts at: 65 1248 4580 4754 65 Size: 1183 3332 174 1396 Fragments arranged by size: 3332 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2196 2315 4729 4916 1700 Size: 110 386 119 2414 187 2869 Fragments arranged by size: 2869 2414 386 187 119 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6085 BssSI C'ACGA_G Cuts at: 2603 4145 5157 2603 Size: 1542 1012 3531 Fragments arranged by size: 3531 1542 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 794 Size: 1412 4673 BstBI TT'CG_AA Cuts at: 4889 4889 Size: 6085 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6085 BstYI r'GATC_y Cuts at: 1972 3273 3415 3782 4167 4184 5636 5722 5734 Size: 1301 142 367 385 17 1452 86 12 Cuts at: 5734 6080 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1301 385 367 346 142 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3078 3078 Size: 6085 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5346 Fragments arranged by size: 5346 683 56 BtsI GCAGTG_nn' Cuts at: 2463 2496 3601 4395 4415 2463 Size: 33 1105 794 20 4133 Fragments arranged by size: 4133 1105 794 33 20 ClaI AT'CG_AT Cuts at: 1554 3363 1554 Size: 1809 4276 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6050 DraI TTT'AAA Cuts at: 3389 3389 Size: 6085 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5092 809 Size: 507 3776 1802 Fragments arranged by size: 3776 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2313 2404 2424 Size: 54 1425 50 275 18 483 91 20 Cuts at: 2424 3532 4421 6050 8 Size: 1108 889 1629 43 Fragments arranged by size: 1629 1425 1108 889 483 275 91 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2313 2424 3532 1537 Size: 275 501 111 1108 4090 Fragments arranged by size: 4090 1108 501 275 111 EarI CTCTTCn'nnn_ Cuts at: 1353 3183 4015 1353 Size: 1830 832 3423 Fragments arranged by size: 3423 1830 832 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2504 4672 5046 5192 387 Size: 1064 138 915 2168 374 146 1280 Fragments arranged by size: 2168 1280 1064 915 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2096 3263 4148 4745 5532 5800 Size: 393 429 35 1167 885 597 787 268 Cuts at: 5800 1239 Size: 1524 Fragments arranged by size: 1524 1167 885 787 597 429 393 268 35 EcoO109I rG'GnC_Cy Cuts at: 2482 2755 2807 3337 2482 Size: 273 52 530 5230 Fragments arranged by size: 5230 530 273 52 EcoRI G'AATT_C Cuts at: 2068 3394 3772 2068 Size: 1326 378 4381 Fragments arranged by size: 4381 1326 378 FspI TGC'GCA Cuts at: 4591 4591 Size: 6085 HaeII r_GCGC'y Cuts at: 1678 5232 1678 Size: 3554 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4726 4758 4800 4832 1373 Size: 32 135 32 3154 32 42 32 2626 Fragments arranged by size: 3154 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3675 669 Size: 988 281 1491 246 3079 Fragments arranged by size: 3079 1491 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6085 HpaI GTT'AAC Cuts at: 3675 3675 Size: 6085 KpnI G_GTAC'C Cuts at: 3410 3410 Size: 6085 MfeI C'AATT_G Cuts at: 3684 3684 Size: 6085 MluI A'CGCG_T Cuts at: 5961 5961 Size: 6085 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5199 5383 1305 Size: 3894 184 2007 Fragments arranged by size: 3894 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2406 10 Size: 54 1425 343 574 3689 Fragments arranged by size: 3689 1425 574 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3230 4043 4402 4561 518 Size: 648 2064 813 359 159 2042 Fragments arranged by size: 2064 2042 813 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2149 2236 2312 2702 3501 4178 5326 5571 Size: 543 87 76 390 799 677 1148 245 Cuts at: 5571 1606 Size: 2120 Fragments arranged by size: 2120 1148 799 677 543 390 245 87 76 NaeI GCC'GGC Cuts at: 2198 2317 2198 Size: 119 5966 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4594 Fragments arranged by size: 4594 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6085 NgoMIV G'CCGG_C Cuts at: 2196 2315 2196 Size: 119 5966 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6085 NotI GC'GGCC_GC Cuts at: 3532 3532 Size: 6085 NspI r_CATG'y Cuts at: 1132 1229 2583 3443 4988 1132 Size: 97 1354 860 1545 2229 Fragments arranged by size: 2229 1545 1354 860 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6085 PciI A'CATG_T Cuts at: 4984 4984 Size: 6085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6085 PmeI GTTT'AAAC Cuts at: 3389 3389 Size: 6085 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4154 4186 5667 5699 4154 Size: 32 1481 32 4540 Fragments arranged by size: 4540 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2755 2755 Size: 6085 PshAI GACnn'nnGTC Cuts at: 1613 3096 1613 Size: 1483 4602 PsiI TTA'TAA Cuts at: 3655 3655 Size: 6085 PspOMI G'GGCC_C Cuts at: 2806 2806 Size: 6085 PstI C_TGCA'G Cuts at: 830 2772 3437 830 Size: 1942 665 3478 Fragments arranged by size: 3478 1942 665 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6085 PvuII CAG'CTG Cuts at: 2236 2236 Size: 6085 SalI G'TCGA_C Cuts at: 1936 3427 1936 Size: 1491 4594 SbfI CC_TGCA'GG Cuts at: 2772 3437 2772 Size: 665 5420 ScaI AGT'ACT Cuts at: 2303 4333 6045 2303 Size: 2030 1712 2343 Fragments arranged by size: 2343 2030 1712 SexAI A'CCwGG_T Cuts at: 2508 2565 2508 Size: 57 6028 SfcI C'TryA_G Cuts at: 826 1045 2191 2768 3433 4568 5249 5440 826 Size: 219 1146 577 665 1135 681 191 1471 Fragments arranged by size: 1471 1146 1135 681 665 577 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2331 2423 2331 Size: 92 5993 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6085 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6085 SmaI CCC'GGG Cuts at: 1872 2015 3412 1872 Size: 143 1397 4545 Fragments arranged by size: 4545 1397 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 3297 4189 5090 5352 820 Size: 197 926 1354 892 901 262 1553 Fragments arranged by size: 1553 1354 926 901 892 262 197 SnaBI TAC'GTA Cuts at: 493 3876 493 Size: 3383 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6085 SphI G_CATG'C Cuts at: 1132 3443 1132 Size: 2311 3774 SspI AAT'ATT Cuts at: 5 52 4009 5 Size: 47 3957 2081 Fragments arranged by size: 3957 2081 47 StuI AGG'CCT Cuts at: 1517 2417 2774 3076 1517 Size: 900 357 302 4526 Fragments arranged by size: 4526 900 357 302 StyI C'CwwG_G Cuts at: 513 1065 2004 2329 2412 2775 3524 513 Size: 552 939 325 83 363 749 3074 Fragments arranged by size: 3074 939 749 552 363 325 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4112 4129 4282 4467 4912 325 Size: 1255 2532 17 153 185 445 1498 Fragments arranged by size: 2532 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 4331 6043 Size: 274 80 33 51 1443 324 2030 1712 Cuts at: 6043 96 Size: 138 Fragments arranged by size: 2030 1712 1443 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6045 6077 6045 Size: 32 6053 Tth111I GACn'n_nGTC Cuts at: 1316 2510 1316 Size: 1194 4891 XbaI T'CTAG_A Cuts at: 3421 3421 Size: 6085 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2384 2563 2657 2384 Size: 179 94 5812 Fragments arranged by size: 5812 179 94 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6085 XmaI C'CCGG_G Cuts at: 1870 2013 3410 1870 Size: 143 1397 4545 Fragments arranged by size: 4545 1397 143 XmnI GAAnn'nnTTC Cuts at: 4214 4214 Size: 6085 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3070 3893 276 Size: 53 83 186 936 1536 823 2468 Fragments arranged by size: 2468 1536 936 823 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI