Restriction map for
pFN21AB9866
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BamHI BcgI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 128 ACGTGT A^C[GA][CT]GT
AlwI 751 GATCC GGATC(4/5)
ApaI 866 GGGCCC GGGCC^C
AspHI 46 GAGCAC G[AT]GC[AT]^C
BalI 1298 TGGCCA TGG^CCA
BanI 1110 GGCGCC G^G[CT][GA]CC
BbeI 1110 GGCGCC GGCGC^C
BbrPI 283 CACGTG CAC^GTG
BbvCI 1096 GCTGAGG CCTCAGC(-5/-2)
BfaI 898 CTAG C^TAG
BglII 662 AGATCT A^GATCT
BsaAI 283 CACGTG [CT]AC^GT[GA]
BsaI 1235 GAGACC GGTCTC(1/5)
BsaWI 1287 ACCGGA [AT]^CCGG[AT]
BsgI 289 CTGCAC GTGCAG(16/14)
BsiHKAI 46 GAGCAC G[AT]GC[AT]^C
BsmAI 1235 GAGAC GTCTC(1/5)
BsrFI 493 GCCGGC [GA]^CCGG[CT]
BssHII 913 GCGCGC G^CGCGC
BssSI 1090 CACGAG CACGAG(-5/-1)
BstAPI 291 GCACATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 560 CCAACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 662 AGATCT [GA]^GATC[CT]
Cfr10I 493 GCCGGC [GA]^CCGG[CT]
DraIII 292 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 365 CCAAGG C^C[AT][AT]GG
HinfI 26 GACTC G^A[ACGT]TC
HphI 155 TCACC GGTGA(8/7)
KasI 1110 GGCGCC G^GCGCC
MaeI 898 CTAG C^TAG
MflI 662 AGATCT [GA]^GATC[CT]
MluNI 1298 TGGCCA TGG^CCA
MscI 1298 TGGCCA TGG^CCA
NaeI 493 GCCGGC GCC^GGC
NarI 1110 GGCGCC GG^CGCC
NgoMIV 493 GCCGGC G^CCGGC
NheI 897 GCTAGC G^CTAGC
NspI 653 ACATGC [GA]CATG^[CT]
PleI 26 GACTC GAGTC(4/5)
PmaCI 283 CACGTG CAC^GTG
PmlI 283 CACGTG CAC^GTG
PpuMI 1165 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 340 CAGCTG CAG^CTG
ScaI 500 AGTACT AGT^ACT
SfoI 1110 GGCGCC GGC^GCC
SmaI 862 CCCGGG CCC^GGG
StyI 365 CCAAGG C^C[AT][AT]GG
XhoII 662 AGATCT [GA]^GATC[CT]
XmaCI 862 CCCGGG C^CCGGG
XmaI 862 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 721 GACGCC G[GA]^CG[CT]C
1110 GGCGCC
AfaI 501 GTAC GT^AC
510 GTAC
AvaI 862 CCCGGG C^[CT]CG[GA]G
1171 CCCGAG
AvaII 440 GGACC G^G[AT]CC
1166 GGACC
BanII 354 GAGCCC G[GA]GC[CT]^C
866 GGGCCC
BsaHI 721 GACGCC G[GA]^CG[CT]C
1110 GGCGCC
BsoBI 862 CCCGGG C^[CT]CG[GA]G
1171 CCCGAG
BspMI 157 ACCTGC ACCTGC(4/8)
959 ACCTGC
BstF5I 222 CATCC GGATG(2/0)
1125 GGATG
EagI 778 CGGCCG C^GGCCG
995 CGGCCG
EarI 307 GAAGAG CTCTTC(1/4)
345 GAAGAG
EclXI 778 CGGCCG C^GGCCG
995 CGGCCG
Eco52I 778 CGGCCG C^GGCCG
995 CGGCCG
EcoNI 834 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
903 CCTGACCGAGG
FokI 222 CATCC GGATG(9/13)
1125 GGATG
Hin1I 721 GACGCC G[GA]^CG[CT]C
1110 GGCGCC
Ksp632I 307 GAAGAG CTCTTC(1/4)
345 GAAGAG
MaeIII 700 GTGAC ^GT[ACGT]AC
1192 GTGAC
PshAI 441 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1237 GACCCAGGTC
PstI 238 CTGCAG CTGCA^G
1046 CTGCAG
RsaI 501 GTAC GT^AC
510 GTAC
SfaNI 1209 GATGC GCATC(5/9)
98 GCATC
SfcI 238 CTGCAG C^T[GA][CT]AG
1046 CTGCAG
SfiI 1248 GGCCAGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1257 GGCCAGCAGGGCC
SmlI 688 CTCAAG C^T[CT][GA]AG
1018 CTCAAG
TaqI 476 TCGA T^CGA
518 TCGA
Tsp45I 700 GTGAC ^GT[GC]AC
1192 GTGAC
TthHB8I 476 TCGA T^CGA
518 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI AviII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaJI BseRI BsiEI BsiYI
BslI BsmFI Bsp1286I BsrBI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI KspI
MaeII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV SacII
Sau3AI Sau96I ScrFI TseI