(Circular) (Six-base) MAPSORT of: pFN21AB9866.seq Check: 4033 from: 1 to: 6010 pFN21AB9866 6010 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2171 2973 2171 Size: 802 5208 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2369 2549 4073 5457 5725 1632 Size: 737 180 1524 1384 268 1917 Fragments arranged by size: 1917 1524 1384 737 268 180 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 2133 4909 5886 1203 Size: 930 2776 977 1327 Fragments arranged by size: 2776 1327 977 930 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2126 2847 1184 Size: 942 721 4347 Fragments arranged by size: 4347 942 721 AlwNI CAG_nnn'CTG Cuts at: 3753 5325 3753 Size: 1572 4438 ApaI G_GGCC'C Cuts at: 2875 2875 Size: 6010 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 3319 3545 3697 5806 1493 Size: 1826 226 152 2109 1697 Fragments arranged by size: 2109 1826 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2867 3176 3335 1870 Size: 73 924 309 159 4545 Fragments arranged by size: 4545 924 309 159 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3115 3331 4786 618 Size: 325 217 627 1328 216 1455 1842 Fragments arranged by size: 1842 1455 1328 627 325 217 216 BanII G_rGCy'C Cuts at: 1391 2363 2875 3329 1391 Size: 972 512 454 4072 Fragments arranged by size: 4072 972 512 454 BbeI G_GCGC'C Cuts at: 3119 3119 Size: 6010 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2035 2446 2609 928 Size: 971 136 411 163 4329 Fragments arranged by size: 4329 971 411 163 136 BbvCI CC'TCA_GC Cuts at: 3102 3102 Size: 6010 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4201 4235 1903 Size: 34 2264 34 3678 Fragments arranged by size: 3678 2264 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2718 2735 2996 Size: 107 122 71 631 605 1046 17 261 Cuts at: 2996 3069 3260 3269 4621 136 Size: 73 191 9 1352 1525 Fragments arranged by size: 1525 1352 1046 631 605 261 191 122 107 73 71 17 9 BglII A'GATC_T Cuts at: 2667 6005 2667 Size: 3338 2672 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2875 4071 5227 1790 Size: 1085 1196 1156 2573 Fragments arranged by size: 2573 1196 1156 1085 BmgBI CAC'GTC Cuts at: 1318 2134 1318 Size: 816 5194 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2995 3741 4699 Size: 416 53 83 399 24 1578 746 958 Cuts at: 4699 442 Size: 1753 Fragments arranged by size: 1753 1578 958 746 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2906 1056 Size: 1850 4160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 2572 2684 3170 4670 1239 Size: 796 537 112 486 1500 2579 Fragments arranged by size: 2579 1500 796 537 486 112 Bpu10I CC'TnA_GC Cuts at: 3102 3102 Size: 6010 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2678 3008 4135 5000 5298 2678 Size: 330 1127 865 298 3390 Fragments arranged by size: 3390 1127 865 330 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3234 4673 882 Size: 617 1735 1439 2219 Fragments arranged by size: 2219 1735 1439 617 BsaAI yAC'GTr Cuts at: 493 1204 2290 3801 493 Size: 711 1086 1511 2702 Fragments arranged by size: 2702 1511 1086 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2727 3116 3817 4199 Size: 53 83 186 936 1194 389 701 382 Cuts at: 4199 4834 275 Size: 635 1451 Fragments arranged by size: 1451 1194 936 701 635 389 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3292 4443 4841 5115 5262 5673 3292 Size: 1151 398 274 147 411 3629 Fragments arranged by size: 3629 1151 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 1178 Size: 30 165 30 717 30 5038 Fragments arranged by size: 5038 717 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2351 2354 3194 3197 3200 1186 Size: 1162 3 3 840 3 3 3996 Fragments arranged by size: 3996 1162 840 3 3 3 3 BseYI C'CCAG_C Cuts at: 2113 2172 2363 2743 2774 3059 5213 5709 2113 Size: 59 191 380 31 285 2154 496 2414 Fragments arranged by size: 2414 2154 496 380 285 191 59 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 1163 Size: 573 543 4894 Fragments arranged by size: 4894 573 543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2410 2551 2786 3003 3460 4221 Size: 275 186 409 141 235 217 457 761 Cuts at: 4221 5249 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 457 409 275 235 217 186 141 BsiHKAI G_wGCw'C Cuts at: 2055 4071 4156 5227 2055 Size: 2016 85 1071 2838 Fragments arranged by size: 2838 2016 1071 85 BsmI GAATG_Cn' Cuts at: 3521 3614 3521 Size: 93 5917 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2363 2875 3329 4071 4156 5227 Size: 399 265 308 512 454 742 85 1071 Cuts at: 5227 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 512 454 399 308 265 85 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2171 2973 3365 844 Size: 1327 802 392 3489 Fragments arranged by size: 3489 1327 802 392 BsrBI CCG'CTC Cuts at: 1394 1823 2536 3007 3138 3897 1394 Size: 429 713 471 131 759 3507 Fragments arranged by size: 3507 759 713 471 429 131 BsrDI GCAATG_nn' Cuts at: 65 1248 4505 4679 65 Size: 1183 3257 174 1396 Fragments arranged by size: 3257 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2498 4654 4841 1700 Size: 110 688 2156 187 2869 Fragments arranged by size: 2869 2156 688 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 2918 1925 Size: 993 5017 BssSI C'ACGA_G Cuts at: 3095 4070 5082 3095 Size: 975 1012 4023 Fragments arranged by size: 4023 1012 975 BstAPI GCAn_nnn'nTGC Cuts at: 794 2302 794 Size: 1508 4502 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstXI CCAn_nnnn'nTGG Cuts at: 2572 2572 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2667 3340 3707 4092 4109 5561 5647 5659 Size: 695 673 367 385 17 1452 86 12 Cuts at: 5659 6005 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 695 673 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2058 2121 2410 2595 2856 2886 Size: 552 939 54 63 289 185 261 30 Cuts at: 2886 3204 513 Size: 318 3319 Fragments arranged by size: 3319 939 552 318 289 261 185 63 54 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2157 2479 487 Size: 683 56 931 322 4018 Fragments arranged by size: 4018 931 683 322 56 BtsI GCAGTG_nn' Cuts at: 3526 4320 4340 3526 Size: 794 20 5196 Fragments arranged by size: 5196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DraIII CAC_nnn'GTG Cuts at: 2302 2302 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5017 809 Size: 507 3701 1802 Fragments arranged by size: 3701 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2427 2500 2783 Size: 54 1425 50 275 18 597 73 283 Cuts at: 2783 2953 3000 3303 3457 4346 5975 8 Size: 170 47 303 154 889 1629 43 Fragments arranged by size: 1629 1425 889 597 303 283 275 170 154 73 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2783 3000 3457 1537 Size: 275 971 217 457 4090 Fragments arranged by size: 4090 971 457 275 217 EarI CTCTTCn'nnn_ Cuts at: 1353 2307 2345 3940 1353 Size: 954 38 1595 3423 Fragments arranged by size: 3423 1595 954 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2141 2926 4597 4971 5117 387 Size: 1064 138 552 785 1671 374 146 1280 Fragments arranged by size: 1671 1280 1064 785 552 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2369 2549 2572 2684 3170 4073 Size: 393 403 334 180 23 112 486 903 Cuts at: 4073 4670 5457 5725 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 903 787 597 486 403 393 334 268 180 112 23 EcoNI CCTnn'n_nnAGG Cuts at: 2843 2912 2843 Size: 69 5941 EcoO109I rG'GnC_Cy Cuts at: 2208 2737 2871 2872 3171 2208 Size: 529 134 1 299 5047 Fragments arranged by size: 5047 529 299 134 1 EcoRI G'AATT_C Cuts at: 3319 3697 3319 Size: 378 5632 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2626 2626 Size: 6010 FspI TGC'GCA Cuts at: 2306 2423 2823 4516 2306 Size: 117 400 1693 3800 Fragments arranged by size: 3800 1693 400 117 FspAI rTGC'GCAy Cuts at: 2306 2423 2823 2306 Size: 117 400 5493 Fragments arranged by size: 5493 400 117 HaeII r_GCGC'y Cuts at: 1678 2243 2438 3119 3162 5157 1678 Size: 565 195 681 43 1995 2531 Fragments arranged by size: 2531 1995 681 565 195 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2378 2410 2740 Size: 32 135 32 563 32 211 32 330 Cuts at: 2740 2772 4651 4683 4725 4757 1373 Size: 32 1879 32 42 32 2626 Fragments arranged by size: 2626 1879 563 330 211 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3600 669 Size: 988 281 1416 246 3079 Fragments arranged by size: 3079 1416 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KasI G'GCGC_C Cuts at: 3115 3115 Size: 6010 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5124 5308 1305 Size: 3819 184 2007 Fragments arranged by size: 3819 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3305 10 Size: 54 1425 343 1473 2715 Fragments arranged by size: 2715 1473 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2108 2255 2621 3968 4327 4486 518 Size: 648 942 147 366 1347 359 159 2042 Fragments arranged by size: 2042 1347 942 648 366 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2123 2347 2412 2544 2553 2743 2831 2888 Size: 517 224 65 132 9 190 88 57 Cuts at: 2888 3206 3279 3426 4103 5251 5496 1606 Size: 318 73 147 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 517 318 245 224 190 147 132 88 73 65 57 9 NaeI GCC'GGC Cuts at: 2500 2500 Size: 6010 NarI GG'CG_CC Cuts at: 3116 3116 Size: 6010 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4519 Fragments arranged by size: 4519 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NgoMIV G'CCGG_C Cuts at: 2498 2498 Size: 6010 NheI G'CTAG_C Cuts at: 1052 2902 1052 Size: 1850 4160 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2662 3368 4913 1132 Size: 97 1433 706 1545 2229 Fragments arranged by size: 2229 1545 1433 706 97 PasI CC'CwG_GG Cuts at: 1348 2839 1348 Size: 1491 4519 PciI A'CATG_T Cuts at: 4909 4909 Size: 6010 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6010 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PmlI CAC'GTG Cuts at: 2290 2290 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3171 3171 Size: 6010 PshAI GACnn'nnGTC Cuts at: 1613 2450 3246 1613 Size: 837 796 4377 Fragments arranged by size: 4377 837 796 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PspOMI G'GGCC_C Cuts at: 2871 2871 Size: 6010 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2183 2215 2183 Size: 32 5978 PstI C_TGCA'G Cuts at: 830 2247 3055 3362 830 Size: 1417 808 307 3478 Fragments arranged by size: 3478 1417 808 307 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2347 2347 Size: 6010 SacII CC_GC'GG Cuts at: 2124 2413 2889 3207 2124 Size: 289 476 318 4927 Fragments arranged by size: 4927 476 318 289 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SanDI GG'GwC_CC Cuts at: 3171 3171 Size: 6010 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 2507 4258 5970 2507 Size: 1751 1712 2547 Fragments arranged by size: 2547 1751 1712 SfcI C'TryA_G Cuts at: 826 1045 2243 3051 3358 4493 5174 5365 826 Size: 219 1198 808 307 1135 681 191 1471 Fragments arranged by size: 1471 1198 1135 808 681 307 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3260 3269 3260 Size: 9 6001 SfoI GGC'GCC Cuts at: 3117 3117 Size: 6010 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 2869 3337 1872 Size: 997 468 4545 Fragments arranged by size: 4545 997 468 SmlI C'TyrA_G Cuts at: 820 1017 1943 2693 3023 4114 5015 5277 820 Size: 197 926 750 330 1091 901 262 1553 Fragments arranged by size: 1553 1091 926 901 750 330 262 197 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2004 2370 3449 513 Size: 552 939 366 1079 3074 Fragments arranged by size: 3074 1079 939 552 366 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 2927 4037 4054 4207 4392 4837 Size: 1255 606 741 1110 17 153 185 445 Cuts at: 4837 325 Size: 1498 Fragments arranged by size: 1498 1255 1110 741 606 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2505 4256 5968 Size: 274 80 33 51 1443 528 1751 1712 Cuts at: 5968 96 Size: 138 Fragments arranged by size: 1751 1712 1443 528 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2120 2152 5970 6002 2120 Size: 32 3818 32 2128 Fragments arranged by size: 3818 2128 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 2867 3335 1870 Size: 997 468 4545 Fragments arranged by size: 4545 997 468 XmnI GAAnn'nnTTC Cuts at: 4139 4139 Size: 6010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI NruI NsiI PacI PfoI PspXI RsrII SacI SapI SexAI SrfI SwaI XcmI