(Circular) (Six-base) MAPSORT of: pFN21AB9864.seq Check: 2148 from: 1 to: 5971 pFN21AB9864 5971 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4034 5418 5686 1632 Size: 2402 1384 268 1917 Fragments arranged by size: 2402 1917 1384 268 AfeI AGC'GCT Cuts at: 2198 2198 Size: 5971 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 3231 4870 5847 1203 Size: 2028 1639 977 1327 Fragments arranged by size: 2028 1639 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2409 2790 3714 5286 2409 Size: 381 924 1572 3094 Fragments arranged by size: 3094 1572 924 381 ApaLI G'TGCA_C Cuts at: 2888 4028 5184 2888 Size: 1140 1156 3675 Fragments arranged by size: 3675 1156 1140 ApoI r'AATT_y Cuts at: 1493 3280 3506 3658 5767 1493 Size: 1787 226 152 2109 1697 Fragments arranged by size: 2109 1787 1697 226 152 AscI GG'CGCG_CC Cuts at: 2458 2458 Size: 5971 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3018 3296 1870 Size: 73 1075 278 4545 Fragments arranged by size: 4545 1075 278 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2864 3059 3149 3292 4747 Size: 325 217 627 1077 195 90 143 1455 Cuts at: 4747 618 Size: 1842 Fragments arranged by size: 1842 1455 1077 627 325 217 195 143 90 BanII G_rGCy'C Cuts at: 1391 3290 1391 Size: 1899 4072 BbeI G_GCGC'C Cuts at: 2868 3153 2868 Size: 285 5686 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2547 2714 3035 3044 928 Size: 971 648 167 321 9 3855 Fragments arranged by size: 3855 971 648 321 167 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 2115 1548 Size: 567 5404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 2015 3388 2015 Size: 1373 4598 Bme1580I G_kGCm'C Cuts at: 1790 2138 2892 2956 3062 4032 5188 1790 Size: 348 754 64 106 970 1156 2573 Fragments arranged by size: 2573 1156 970 754 348 106 64 BmgBI CAC'GTC Cuts at: 1318 2095 2353 1318 Size: 777 258 4936 Fragments arranged by size: 4936 777 258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2324 2419 3702 Size: 416 53 83 399 24 907 95 1283 Cuts at: 3702 4660 442 Size: 958 1753 Fragments arranged by size: 1753 1283 958 907 416 399 95 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2812 2894 4631 1239 Size: 1573 82 1737 2579 Fragments arranged by size: 2579 1737 1573 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 4634 882 Size: 617 558 2577 2219 Fragments arranged by size: 2577 2219 617 558 BsaAI yAC'GTr Cuts at: 493 1204 2437 3762 493 Size: 711 1233 1325 2702 Fragments arranged by size: 2702 1325 1233 711 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2450 2865 3150 3778 Size: 53 83 186 936 917 415 285 628 Cuts at: 3778 4160 4795 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 917 635 628 415 382 285 186 83 53 BsaWI w'CCGG_w Cuts at: 2711 4404 4802 5076 5223 5634 2711 Size: 1693 398 274 147 411 3048 Fragments arranged by size: 3048 1693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5746 Fragments arranged by size: 5746 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5971 BseYI C'CCAG_C Cuts at: 2131 2158 2521 2797 2816 2927 3043 3166 5174 Size: 27 363 276 19 111 116 123 2008 Cuts at: 5174 5670 2131 Size: 496 2432 Fragments arranged by size: 2432 2008 496 363 276 123 116 111 27 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2347 1163 Size: 573 611 4787 Fragments arranged by size: 4787 611 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2301 2827 2892 2916 4032 4117 5188 2016 Size: 285 526 65 24 1116 85 1071 2799 Fragments arranged by size: 2799 1116 1071 526 285 85 65 24 BsmI GAATG_Cn' Cuts at: 3482 3575 3482 Size: 93 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2138 2301 2827 2892 2916 2956 Size: 399 226 122 163 526 65 24 40 Cuts at: 2956 3062 3290 4032 4117 5188 1391 Size: 106 228 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 228 226 163 122 106 85 65 40 24 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2336 3326 844 Size: 1492 990 3489 Fragments arranged by size: 3489 1492 990 BsrBI CCG'CTC Cuts at: 1394 1823 2547 2717 3858 1394 Size: 429 724 170 1141 3507 Fragments arranged by size: 3507 1141 724 429 170 BsrDI GCAATG_nn' Cuts at: 65 1248 2170 4466 4640 65 Size: 1183 922 2296 174 1396 Fragments arranged by size: 2296 1396 1183 922 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2050 2066 4615 4802 1700 Size: 110 240 16 2549 187 2869 Fragments arranged by size: 2869 2549 240 187 110 16 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 2458 2669 1925 Size: 533 211 5227 Fragments arranged by size: 5227 533 211 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 2169 794 Size: 1375 4596 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 2242 2323 1184 Size: 1058 81 4832 Fragments arranged by size: 4832 1058 81 BstXI CCAn_nnnn'nTGG Cuts at: 2191 2398 3145 2191 Size: 207 747 5017 Fragments arranged by size: 5017 747 207 BstYI r'GATC_y Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966 Size: 1329 367 385 17 1452 86 12 346 Cuts at: 5966 1972 Size: 1977 Fragments arranged by size: 1977 1452 1329 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2277 2728 513 Size: 552 939 273 451 3756 Fragments arranged by size: 3756 939 552 451 273 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2170 2326 2377 487 Size: 683 56 944 156 51 4081 Fragments arranged by size: 4081 944 683 156 56 51 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DraIII CAC_nnn'GTG Cuts at: 2896 2896 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2099 4978 809 Size: 507 783 2879 1802 Fragments arranged by size: 2879 1802 783 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 2280 3418 Size: 54 1425 50 275 18 384 66 1138 Cuts at: 3418 4307 5936 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1138 889 384 275 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2753 3901 1353 Size: 1400 1148 3423 Fragments arranged by size: 3423 1400 1148 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2812 2894 4034 4631 5418 5686 1239 Size: 393 1180 82 1140 597 787 268 1524 Fragments arranged by size: 1524 1180 1140 787 597 393 268 82 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 2200 2868 3153 5118 1678 Size: 522 668 285 1965 2531 Fragments arranged by size: 2531 1965 668 522 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2107 2139 4612 4644 4686 Size: 32 135 32 535 32 2473 32 42 Cuts at: 4686 4718 1373 Size: 32 2626 Fragments arranged by size: 2626 2473 535 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KasI G'GCGC_C Cuts at: 2864 3149 2864 Size: 285 5686 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3160 3570 3160 Size: 410 5561 MluI A'CGCG_T Cuts at: 3231 5847 3231 Size: 2616 3355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 3201 5085 5269 1305 Size: 810 1086 1884 184 2007 Fragments arranged by size: 2007 1884 1086 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 10 Size: 54 1425 343 384 3765 Fragments arranged by size: 3765 1425 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3053 3929 4288 4447 518 Size: 648 843 1044 876 359 159 2042 Fragments arranged by size: 2042 1044 876 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2465 2626 3126 3387 4064 5212 5457 1606 Size: 859 161 500 261 677 1148 245 2120 Fragments arranged by size: 2120 1148 859 677 500 261 245 161 NarI GG'CG_CC Cuts at: 2865 3150 2865 Size: 285 5686 NcoI C'CATG_G Cuts at: 513 1065 2004 2277 2728 513 Size: 552 939 273 451 3756 Fragments arranged by size: 3756 939 552 451 273 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2565 3136 3329 4874 1132 Size: 97 1336 571 193 1545 2229 Fragments arranged by size: 2229 1545 1336 571 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 2208 3323 830 Size: 1378 1115 3478 Fragments arranged by size: 3478 1378 1115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 3126 3126 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 2204 3319 4454 5135 5326 826 Size: 219 1159 1115 1135 681 191 1471 Fragments arranged by size: 1471 1159 1135 1115 681 219 191 SfoI GGC'GCC Cuts at: 2866 3151 2866 Size: 285 5686 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4075 4976 5238 820 Size: 197 926 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3762 493 Size: 3269 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 2565 3329 1132 Size: 1433 764 3774 Fragments arranged by size: 3774 1433 764 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 2277 2728 3410 513 Size: 552 939 186 87 451 682 3074 Fragments arranged by size: 3074 939 682 552 451 186 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3998 4015 4168 4353 4798 325 Size: 1255 2418 17 153 185 445 1498 Fragments arranged by size: 2418 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI