(Circular) (Six-base) MAPSORT of: pFN21AB9860.seq Check: 1716 from: 1 to: 5923 pFN21AB9860 5923 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3138 3986 5370 5638 1632 Size: 1506 848 1384 268 1917 Fragments arranged by size: 1917 1506 1384 848 268 AfeI AGC'GCT Cuts at: 2429 2429 Size: 5923 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 4822 5799 1203 Size: 3619 977 1327 Fragments arranged by size: 3619 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 2245 1166 Size: 1079 4844 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3182 3214 3182 Size: 32 5891 AlwNI CAG_nnn'CTG Cuts at: 3666 5238 3666 Size: 1572 4351 ApaI G_GGCC'C Cuts at: 2190 2277 2190 Size: 87 5836 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2306 3071 3232 3458 3610 5719 1493 Size: 813 765 161 226 152 2109 1697 Fragments arranged by size: 2109 1697 813 765 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2131 2221 3248 1870 Size: 73 188 90 1027 4545 Fragments arranged by size: 4545 1027 188 90 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2127 2280 3244 4699 618 Size: 325 217 627 340 153 964 1455 1842 Fragments arranged by size: 1842 1455 964 627 340 325 217 153 BanII G_rGCy'C Cuts at: 1391 2105 2190 2277 2489 3242 1391 Size: 714 85 87 212 753 4072 Fragments arranged by size: 4072 753 714 212 87 85 BbeI G_GCGC'C Cuts at: 2131 2284 2131 Size: 153 5770 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 928 Size: 971 138 4814 Fragments arranged by size: 4814 971 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2312 2346 4114 4148 1903 Size: 34 375 34 1768 34 3678 Fragments arranged by size: 3678 1768 375 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2442 4534 136 Size: 107 122 71 631 605 770 2092 1525 Fragments arranged by size: 2092 1525 770 631 605 122 107 71 BglII A'GATC_T Cuts at: 2592 5918 2592 Size: 3326 2597 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2086 2190 2277 3984 5140 1790 Size: 296 104 87 1707 1156 2573 Fragments arranged by size: 2573 1707 1156 296 104 87 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5923 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2266 3654 4612 Size: 416 53 83 399 24 849 1388 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1388 958 849 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2124 2277 2398 3065 4583 1239 Size: 885 153 121 667 1518 2579 Fragments arranged by size: 2579 1518 885 667 153 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2119 4048 4913 5211 2119 Size: 1929 865 298 2831 Fragments arranged by size: 2831 1929 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2128 2281 3730 4112 Size: 53 83 186 936 595 153 1449 382 Cuts at: 4112 4747 275 Size: 635 1451 Fragments arranged by size: 1451 1449 936 635 595 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 2696 4356 4754 5028 5175 5586 2696 Size: 1660 398 274 147 411 3033 Fragments arranged by size: 3033 1660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 1178 Size: 30 165 30 675 30 4993 Fragments arranged by size: 4993 675 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2855 2858 2879 2882 2924 2948 2969 3005 Size: 1669 3 21 3 42 24 21 36 Cuts at: 3005 3014 3017 3029 3032 3050 3062 1186 Size: 9 3 12 3 18 12 4047 Fragments arranged by size: 4047 1669 42 36 24 21 21 18 12 12 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2079 2265 2400 2430 2683 5126 5622 2079 Size: 186 135 30 253 2443 496 2380 Fragments arranged by size: 2443 2380 496 253 186 135 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2786 3130 1163 Size: 573 1050 344 3956 Fragments arranged by size: 3956 1050 573 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2519 3373 4134 5162 1540 Size: 275 186 518 854 761 1028 2301 Fragments arranged by size: 2301 1028 854 761 518 275 186 BsiHKAI G_wGCw'C Cuts at: 2105 2140 2252 2507 3984 4069 5140 2105 Size: 35 112 255 1477 85 1071 2888 Fragments arranged by size: 2888 1477 1071 255 112 85 35 BsmI GAATG_Cn' Cuts at: 2783 3434 3527 2783 Size: 651 93 5179 Fragments arranged by size: 5179 651 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2105 2140 2190 2252 2277 2489 Size: 399 296 19 35 50 62 25 212 Cuts at: 2489 2507 3242 3984 4069 5140 1391 Size: 18 735 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 735 399 296 212 85 62 50 35 25 19 18 BspEI T'CCGG_A Cuts at: 2696 5586 2696 Size: 2890 3033 BspHI T'CATG_A Cuts at: 2583 3812 5542 2583 Size: 1229 1730 2964 Fragments arranged by size: 2964 1730 1229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2382 2670 3810 1394 Size: 429 559 288 1140 3507 Fragments arranged by size: 3507 1140 559 429 288 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2518 4567 4754 1700 Size: 110 708 2049 187 2869 Fragments arranged by size: 2869 2049 708 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2301 2301 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2592 2690 3253 3620 4005 4022 5474 5560 Size: 620 98 563 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 620 563 385 367 346 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2446 2480 2513 2661 513 Size: 552 1381 34 33 148 3775 Fragments arranged by size: 3775 1381 552 148 34 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3439 4233 4253 3439 Size: 794 20 5109 Fragments arranged by size: 5109 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3156 3227 3156 Size: 71 5852 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2516 3370 4259 Size: 54 1425 50 275 18 686 854 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 854 686 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2516 3370 1537 Size: 275 704 854 4090 Fragments arranged by size: 4090 854 704 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2832 2913 2934 2958 2967 3006 3021 3027 Size: 1479 81 21 24 9 39 15 6 Cuts at: 3027 3853 1353 Size: 826 3423 Fragments arranged by size: 3423 1479 826 81 39 24 21 15 9 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2103 2103 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2277 2398 3065 3138 3986 4583 Size: 393 492 153 121 667 73 848 597 Cuts at: 4583 5370 5638 1239 Size: 787 268 1524 Fragments arranged by size: 1524 848 787 667 597 492 393 268 153 121 73 EcoO109I rG'GnC_Cy Cuts at: 2187 2273 2274 2385 3040 2187 Size: 86 1 111 655 5070 Fragments arranged by size: 5070 655 111 86 1 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3165 3165 Size: 5923 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2131 2284 2431 2561 2645 5070 1678 Size: 453 153 147 130 84 2425 2531 Fragments arranged by size: 2531 2425 453 153 147 130 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373 Size: 32 135 32 2992 32 42 32 2626 Fragments arranged by size: 2992 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2127 2280 2127 Size: 153 5770 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2260 5037 5221 1305 Size: 955 2777 184 2007 Fragments arranged by size: 2777 2007 955 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2245 3881 4240 4399 518 Size: 648 1079 1636 359 159 2042 Fragments arranged by size: 2042 1636 1079 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2448 2482 2495 2515 2824 3339 4016 5164 Size: 842 34 13 20 309 515 677 1148 Cuts at: 5164 5409 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 842 677 515 309 245 34 20 13 NarI GG'CG_CC Cuts at: 2128 2281 2128 Size: 153 5770 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5371 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2554 3281 4826 1132 Size: 97 1325 727 1545 2229 Fragments arranged by size: 2229 1545 1325 727 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PfoI T'CCnGG_A Cuts at: 2293 2387 3042 2293 Size: 94 655 5174 Fragments arranged by size: 5174 655 94 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2385 3040 2385 Size: 655 5268 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3140 3493 3140 Size: 353 5570 PspOMI G'GGCC_C Cuts at: 2186 2273 2186 Size: 87 5836 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2579 2611 2579 Size: 32 5891 PstI C_TGCA'G Cuts at: 830 2745 3275 830 Size: 1915 530 3478 Fragments arranged by size: 3478 1915 530 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 SacI G_AGCT'C Cuts at: 2105 2105 Size: 5923 SacII CC_GC'GG Cuts at: 2449 2483 2516 2449 Size: 34 33 5856 Fragments arranged by size: 5856 34 33 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2741 3271 4406 5087 5278 826 Size: 219 1696 530 1135 681 191 1471 Fragments arranged by size: 1696 1471 1135 681 530 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2442 2442 Size: 5923 SfoI GGC'GCC Cuts at: 2129 2282 2129 Size: 153 5770 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2133 2223 3250 1872 Size: 261 90 1027 4545 Fragments arranged by size: 4545 1027 261 90 SmlI C'TyrA_G Cuts at: 820 1017 1943 2098 4027 4928 5190 820 Size: 197 926 155 1929 901 262 1553 Fragments arranged by size: 1929 1553 926 901 262 197 155 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 2554 3281 1132 Size: 1422 727 3774 Fragments arranged by size: 3774 1422 727 SrfI GCCC'GGGC Cuts at: 2223 2223 Size: 5923 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 2678 1517 Size: 1161 4762 StyI C'CwwG_G Cuts at: 513 1065 2192 3362 513 Size: 552 1127 1170 3074 Fragments arranged by size: 3074 1170 1127 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 2392 2588 3950 3967 4120 4305 Size: 1255 612 200 196 1362 17 153 185 Cuts at: 4305 4750 325 Size: 445 1498 Fragments arranged by size: 1498 1362 1255 612 445 200 196 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 5883 5915 2078 Size: 32 3773 32 2086 Fragments arranged by size: 3773 2086 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2131 2221 3248 1870 Size: 261 90 1027 4545 Fragments arranged by size: 4545 1027 261 90 XmnI GAAnn'nnTTC Cuts at: 3072 4052 3072 Size: 980 4943 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PvuII RsrII SanDI SapI SexAI SwaI XcmI