Restriction map for
pFN21AB9857
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BsaAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoT22I FseI FspI
HindIII HpaI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 309 GACGTC GACGT^C
AccI 280 GTAGAC GT^[AC][GT]AC
AccIII 1004 TCCGGA T^CCGGA
AfaI 15 GTAC GT^AC
AlwNI 541 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1084 CCTGAGG CC^T[ACGT]AGG
BbeI 784 GGCGCC GGCGC^C
BbsI 1134 GTCTTC GAAGAC(2/6)
BclI 5 TGATCA T^GATCA
BlpI 513 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 513 GCTCAGC GC^T[ACGT]AGC
BpuAI 1134 GTCTTC GAAGAC(2/6)
BsaBI 132 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 529 GAGACC GGTCTC(1/5)
BsaWI 1004 TCCGGA [AT]^CCGG[AT]
BseAI 1004 TCCGGA T^CCGGA
BseRI 157 CTCCTC GAGGAG(10/8)
BsmBI 595 CGTCTC CGTCTC(1/5)
BspDI 1024 ATCGAT AT^CGAT
BspEI 1004 TCCGGA T^CCGGA
BspHI 833 TCATGA T^CATGA
BsrDI 294 CATTGC GCAATG(2/0)
BssSI 275 CTCGTG CACGAG(-5/-1)
BstEII 42 GGTCACC G^GT[ACGT]ACC
BstPI 42 GGTCACC G^GT[ACGT]ACC
BstYI 131 AGATCC [GA]^GATC[CT]
Bsu36I 1084 CCTGAGG CC^T[ACGT]AGG
CelII 513 GCTCAGC GC^T[ACGT]AGC
ClaI 1024 ATCGAT AT^CGAT
DsaI 974 CCATGG C^C[GA][CT]GG
EaeI 897 TGGCCG [CT]^GGCC[GA]
Eco81I 1084 CCTGAGG CC^T[ACGT]AGG
EcoO65I 42 GGTCACC G^GT[ACGT]ACC
EcoRV 208 GATATC GAT^ATC
FbaI 5 TGATCA T^GATCA
HaeII 784 GGCGCC [GA]GCGC^[CT]
HgaI 385 GCGTC GACGC(5/10)
HincII 775 GTTGAC GT[CT]^[GA]AC
HindII 775 GTTGAC GT[CT]^[GA]AC
KasI 784 GGCGCC G^GCGCC
MaeII 310 ACGT A^CGT
MamI 132 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 131 AGATCC [GA]^GATC[CT]
MroI 1004 TCCGGA T^CCGGA
MseI 737 TTAA T^TAA
NarI 784 GGCGCC GG^CGCC
NcoI 974 CCATGG C^CATGG
NspI 443 GCATGC [GA]CATG^[CT]
PstI 223 CTGCAG CTGCA^G
RcaI 833 TCATGA T^CATGA
RsaI 15 GTAC GT^AC
SacI 76 GAGCTC GAGCT^C
SfcI 223 CTGCAG C^T[GA][CT]AG
SfoI 784 GGCGCC GGC^GCC
SphI 443 GCATGC GCATG^C
Tru9I 737 TTAA T^TAA
XhoII 131 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspHI 76 GAGCTC G[AT]GC[AT]^C
945 GTGCTC
BpmI 55 CTCCAG CTGGAG(16/14)
251 CTCCAG
BsiHKAI 76 GAGCTC G[AT]GC[AT]^C
945 GTGCTC
BsrI 615 ACTGG ACTGG(1/-1)
1060 ACTGG
CfoI 351 GCGC GCG^C
785 GCGC
EcoT14I 497 CCTTGG C^C[AT][AT]GG
974 CCATGG
FauI 358 CCCGC CCCGC(4/6)
427 CCCGC
HapII 654 CCGG C^CGG
1005 CCGG
HhaI 351 GCGC GCG^C
785 GCGC
HinP1I 351 GCGC G^CGC
785 GCGC
HpaII 654 CCGG C^CGG
1005 CCGG
MspI 654 CCGG C^CGG
1005 CCGG
SfaNI 726 GATGC GCATC(5/9)
756 GCATC
StyI 497 CCTTGG C^C[AT][AT]GG
974 CCATGG
TfiI 918 GATTC G^A[AT]TC
1168 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaI
AvaII BanI BanII BbvI BfaI
BmyI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeIII Hin1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SmlI StuI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I