(Circular) (Six-base) MAPSORT of: pFN21AB9857.seq Check: 5734 from: 1 to: 5893 pFN21AB9857 5893 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2318 3703 278 Size: 53 83 186 936 782 1385 2468 Fragments arranged by size: 2468 1385 936 782 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 3236 1937 Size: 349 950 4594 Fragments arranged by size: 4594 950 349 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2275 3956 5340 5608 1632 Size: 643 1681 1384 268 1917 Fragments arranged by size: 1917 1681 1384 643 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5893 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2321 2468 1184 Size: 1137 147 4609 Fragments arranged by size: 4609 1137 147 AlwNI CAG_nnn'CTG Cuts at: 2551 3636 5208 2551 Size: 1085 1572 3236 Fragments arranged by size: 3236 1572 1085 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 3202 3428 3580 5689 1493 Size: 1709 226 152 2109 1697 Fragments arranged by size: 2109 1709 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 2250 2708 2785 3218 1870 Size: 73 307 458 77 433 4545 Fragments arranged by size: 4545 458 433 307 77 73 BamHI G'GATC_C Cuts at: 3223 3590 3223 Size: 367 5526 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2789 3059 3214 4669 Size: 325 217 627 518 484 270 155 1455 Cuts at: 4669 618 Size: 1842 Fragments arranged by size: 1842 1455 627 518 484 325 270 217 155 BanII G_rGCy'C Cuts at: 1391 2085 2192 2257 3212 1391 Size: 694 107 65 955 4072 Fragments arranged by size: 4072 955 694 107 65 BbeI G_GCGC'C Cuts at: 2793 2793 Size: 5893 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3132 928 Size: 971 1233 3689 Fragments arranged by size: 3689 1233 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4084 4118 1903 Size: 34 50 34 2063 34 3678 Fragments arranged by size: 3678 2063 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3785 4995 3785 Size: 1210 4683 BclI T'GATC_A Cuts at: 1548 2010 1548 Size: 462 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5888 5888 Size: 5893 BlpI GC'TnA_GC Cuts at: 2519 3310 2519 Size: 791 5102 Bme1580I G_kGCm'C Cuts at: 1790 2269 2888 3954 5110 1790 Size: 479 619 1066 1156 2573 Fragments arranged by size: 2573 1156 1066 619 479 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3624 4582 442 Size: 416 53 83 399 24 2207 958 1753 Fragments arranged by size: 2207 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2241 4553 1239 Size: 806 196 2312 2579 Fragments arranged by size: 2579 2312 806 196 Bpu10I CC'TnA_GC Cuts at: 2634 2634 Size: 5893 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 2552 2599 2987 4018 4883 5181 2468 Size: 84 47 388 1031 865 298 3180 Fragments arranged by size: 3180 1031 865 388 298 84 47 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2528 4556 882 Size: 617 1029 2028 2219 Fragments arranged by size: 2219 2028 1029 617 BsaAI yAC'GTr Cuts at: 493 1204 3684 493 Size: 711 2480 2702 Fragments arranged by size: 2702 2480 711 BsaBI GATnn'nnATC Cuts at: 2141 5887 2141 Size: 3746 2147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2315 2390 2790 3700 Size: 53 83 186 936 782 75 400 910 Cuts at: 3700 4082 4717 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 910 782 635 400 382 186 83 75 53 BsaWI w'CCGG_w Cuts at: 3009 4326 4724 4998 5145 5556 3009 Size: 1317 398 274 147 411 3346 Fragments arranged by size: 3346 1317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2388 2418 1178 Size: 30 165 30 985 30 4653 Fragments arranged by size: 4653 985 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 1186 Size: 967 4926 BseYI C'CCAG_C Cuts at: 2370 2864 5096 5592 2370 Size: 494 2232 496 2671 Fragments arranged by size: 2671 2232 496 494 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2954 3954 4039 5110 2085 Size: 869 1000 85 1071 2868 Fragments arranged by size: 2868 1071 1000 869 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 BsmBI CGTCTCn'nnnn_ Cuts at: 2606 2606 Size: 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2192 2257 2269 2888 2954 3212 Size: 399 295 107 65 12 619 66 258 Cuts at: 3212 3954 4039 5110 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 619 399 295 258 107 85 66 65 12 BspEI T'CCGG_A Cuts at: 3009 5556 3009 Size: 2547 3346 BspHI T'CATG_A Cuts at: 2838 3782 5512 2838 Size: 944 1730 3219 Fragments arranged by size: 3219 1730 944 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3248 844 Size: 2404 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2298 4388 4562 65 Size: 1183 1050 2090 174 1396 Fragments arranged by size: 2090 1396 1183 1050 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4537 4724 1700 Size: 110 2727 187 2869 Fragments arranged by size: 2869 2727 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 2280 3953 4965 2280 Size: 1673 1012 3208 Fragments arranged by size: 3208 1673 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 2047 1184 Size: 863 5030 BstYI r'GATC_y Cuts at: 1972 2136 3223 3590 3975 3992 5444 5530 5542 Size: 164 1087 367 385 17 1452 86 12 Cuts at: 5542 5888 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1087 385 367 346 164 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3090 3090 Size: 5893 BtgI C'CryG_G Cuts at: 513 1065 2979 513 Size: 552 1914 3427 Fragments arranged by size: 3427 1914 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2842 487 Size: 683 56 1616 3538 Fragments arranged by size: 3538 1616 683 56 BtsI GCAGTG_nn' Cuts at: 3409 4203 4223 3409 Size: 794 20 5079 Fragments arranged by size: 5079 794 20 ClaI AT'CG_AT Cuts at: 1554 3030 1554 Size: 1476 4417 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4900 809 Size: 507 3584 1802 Fragments arranged by size: 3584 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2902 3340 4229 Size: 54 1425 50 275 18 1072 438 889 Cuts at: 4229 5858 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1072 889 438 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2643 2694 3823 1353 Size: 774 516 51 1129 3423 Fragments arranged by size: 3423 1129 774 516 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2522 4480 4854 5000 387 Size: 1064 138 933 1958 374 146 1280 Fragments arranged by size: 1958 1280 1064 933 374 146 138 EcoICRI GAG'CTC Cuts at: 2083 2083 Size: 5893 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2241 2275 3956 4553 5340 5608 Size: 393 413 196 34 1681 597 787 268 Cuts at: 5608 1239 Size: 1524 Fragments arranged by size: 1681 1524 787 597 413 393 268 196 34 EcoNI CCTnn'n_nnAGG Cuts at: 2042 2168 2483 2042 Size: 126 315 5452 Fragments arranged by size: 5452 315 126 EcoO109I rG'GnC_Cy Cuts at: 2035 2098 2246 2704 2035 Size: 63 148 458 5224 Fragments arranged by size: 5224 458 148 63 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 EcoRV GAT'ATC Cuts at: 2215 2215 Size: 5893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2612 2612 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 2793 5040 1678 Size: 1115 2247 2531 Fragments arranged by size: 2531 2247 1115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2112 2144 2151 2183 2527 Size: 32 135 32 540 32 7 32 344 Cuts at: 2527 2559 2584 2616 2820 2852 4534 4566 4608 Size: 32 25 32 204 32 1682 32 42 Cuts at: 4608 4640 1373 Size: 32 2626 Fragments arranged by size: 2626 1682 540 344 204 135 42 32 32 32 32 32 32 32 32 32 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 2782 3237 3483 669 Size: 988 281 844 455 246 3079 Fragments arranged by size: 3079 988 844 455 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KasI G'GCGC_C Cuts at: 2789 2789 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2484 5007 5191 1305 Size: 1179 2523 184 2007 Fragments arranged by size: 2523 2007 1179 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2303 2984 3851 4210 4369 518 Size: 648 984 153 681 867 359 159 2042 Fragments arranged by size: 2042 984 867 681 648 359 159 153 MspA1I CmG'CkG Cuts at: 1606 2366 2374 2523 3309 3986 5134 5379 1606 Size: 760 8 149 786 677 1148 245 2120 Fragments arranged by size: 2120 1148 786 760 677 245 149 8 NarI GG'CG_CC Cuts at: 2790 2790 Size: 5893 NcoI C'CATG_G Cuts at: 513 1065 2979 513 Size: 552 1914 3427 Fragments arranged by size: 3427 1914 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 2452 3194 3251 4796 1132 Size: 97 1223 742 57 1545 2229 Fragments arranged by size: 2229 1545 1223 742 97 57 PasI CC'CwG_GG Cuts at: 1348 2185 1348 Size: 837 5056 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5893 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2035 2098 2246 2035 Size: 63 148 5682 Fragments arranged by size: 5682 148 63 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5893 PsiI TTA'TAA Cuts at: 3463 3463 Size: 5893 PstI C_TGCA'G Cuts at: 830 2232 3245 830 Size: 1402 1013 3478 Fragments arranged by size: 3478 1402 1013 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 SacI G_AGCT'C Cuts at: 2085 2085 Size: 5893 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 2228 3241 4376 5057 5248 826 Size: 219 1183 1013 1135 681 191 1471 Fragments arranged by size: 1471 1183 1135 1013 681 219 191 SfoI GGC'GCC Cuts at: 2791 2791 Size: 5893 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3220 1872 Size: 1348 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 2531 2578 2966 3997 4898 Size: 197 926 540 48 47 388 1031 901 Cuts at: 4898 5160 820 Size: 262 1553 Fragments arranged by size: 1553 1031 926 901 540 388 262 197 48 47 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 2452 3251 1132 Size: 1320 799 3774 Fragments arranged by size: 3774 1320 799 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 2412 2576 2997 1517 Size: 895 164 421 4413 Fragments arranged by size: 4413 895 421 164 StyI C'CwwG_G Cuts at: 513 1065 2502 2979 3332 513 Size: 552 1437 477 353 3074 Fragments arranged by size: 3074 1437 552 477 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2868 3920 3937 4090 4275 4720 325 Size: 1255 1288 1052 17 153 185 445 1498 Fragments arranged by size: 1498 1288 1255 1052 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2019 4139 5851 Size: 274 80 33 51 1443 42 2120 1712 Cuts at: 5851 96 Size: 138 Fragments arranged by size: 2120 1712 1443 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 2459 2491 2843 2875 3109 3141 5853 Size: 32 39 32 352 32 234 32 2712 Cuts at: 5853 5885 2388 Size: 32 2396 Fragments arranged by size: 2712 2396 352 234 39 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 3229 3229 Size: 5893 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3218 1870 Size: 1348 4545 XmnI GAAnn'nnTTC Cuts at: 4022 4022 Size: 5893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2316 3701 276 Size: 53 83 186 936 782 1385 2468 Fragments arranged by size: 2468 1385 936 782 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI