Restriction map for
pFN21AB9857
(Linear) (Six-base) MAPSORT of: KIBB9857.seq Check: 1048 from: 1 to: 1188
KIBB9857.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AatII G_ACGT'C
Cuts at: 0 313 1188
Size: 313 875
AccI GT'mk_AC
Cuts at: 0 281 1188
Size: 281 907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 1188
Size: 270 918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 463 1188
Size: 316 147 725
Fragments arranged by size:
725 316 147
AlwNI CAG_nnn'CTG
Cuts at: 0 546 1188
Size: 546 642
AvaI C'yCGr_G
Cuts at: 0 245 703 780 1188
Size: 245 458 77 408
Fragments arranged by size:
458 408 245 77
BanI G'GyrC_C
Cuts at: 0 300 784 1054 1188
Size: 300 484 270 134
Fragments arranged by size:
484 300 270 134
BanII G_rGCy'C
Cuts at: 0 80 187 252 1188
Size: 80 107 65 936
Fragments arranged by size:
936 107 80 65
BbeI G_GCGC'C
Cuts at: 0 788 1188
Size: 788 400
BbsI GAAGACnn'nnnn_
Cuts at: 0 1127 1188
Size: 1127 61
BclI T'GATC_A
Cuts at: 0 5 1188
Size: 5 1183
BlpI GC'TnA_GC
Cuts at: 0 514 1188
Size: 514 674
Bme1580I G_kGCm'C
Cuts at: 0 264 883 1188
Size: 264 619 305
Fragments arranged by size:
619 305 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 236 1188
Size: 40 196 952
Fragments arranged by size:
952 196 40
Bpu10I CC'TnA_GC
Cuts at: 0 629 1188
Size: 629 559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 547 594 982 1188
Size: 463 84 47 388 206
Fragments arranged by size:
463 388 206 84 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 523 1188
Size: 523 665
BsaBI GATnn'nnATC
Cuts at: 0 136 1188
Size: 136 1052
BsaHI Gr'CG_yC
Cuts at: 0 310 385 785 1188
Size: 310 75 400 403
Fragments arranged by size:
403 400 310 75
BsaWI w'CCGG_w
Cuts at: 0 1004 1188
Size: 1004 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 383 413 1188
Size: 383 30 775
Fragments arranged by size:
775 383 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 1188
Size: 148 1040
BseYI C'CCAG_C
Cuts at: 0 365 859 1188
Size: 365 494 329
Fragments arranged by size:
494 365 329
BsiHKAI G_wGCw'C
Cuts at: 0 80 949 1188
Size: 80 869 239
Fragments arranged by size:
869 239 80
BsmBI CGTCTCn'nnnn_
Cuts at: 0 601 1188
Size: 601 587
Bsp1286I G_dGCh'C
Cuts at: 0 80 187 252 264 883 949 1188
Size: 80 107 65 12 619 66 239
Fragments arranged by size:
619 239 107 80 66 65 12
BspEI T'CCGG_A
Cuts at: 0 1004 1188
Size: 1004 184
BspHI T'CATG_A
Cuts at: 0 833 1188
Size: 833 355
BsrDI GCAATG_nn'
Cuts at: 0 293 1188
Size: 293 895
BssSI C'ACGA_G
Cuts at: 0 275 1188
Size: 275 913
BstEII G'GTnAC_C
Cuts at: 0 42 1188
Size: 42 1146
BstYI r'GATC_y
Cuts at: 0 131 1188
Size: 131 1057
Bsu36I CC'TnA_GG
Cuts at: 0 1085 1188
Size: 1085 103
BtgI C'CryG_G
Cuts at: 0 974 1188
Size: 974 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 837 1188
Size: 837 351
ClaI AT'CG_AT
Cuts at: 0 1025 1188
Size: 1025 163
EaeI y'GGCC_r
Cuts at: 0 897 1188
Size: 897 291
EarI CTCTTCn'nnn_
Cuts at: 0 122 638 689 1188
Size: 122 516 51 499
Fragments arranged by size:
516 499 122 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 517 1188
Size: 517 671
EcoICRI GAG'CTC
Cuts at: 0 78 1188
Size: 78 1110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 236 270 1188
Size: 40 196 34 918
Fragments arranged by size:
918 196 40 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 37 163 478 1188
Size: 37 126 315 710
Fragments arranged by size:
710 315 126 37
EcoO109I rG'GnC_Cy
Cuts at: 0 30 93 241 699 1188
Size: 30 63 148 458 489
Fragments arranged by size:
489 458 148 63 30
EcoRV GAT'ATC
Cuts at: 0 210 1188
Size: 210 978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 607 1188
Size: 607 581
HaeII r_GCGC'y
Cuts at: 0 788 1188
Size: 788 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 146 178 522 554 579 611
Size: 107 32 7 32 344 32 25 32
Cuts at: 611 815 847 1188
Size: 204 32 341
Fragments arranged by size:
344 341 204 107 32 32 32 32
32 25 7
HincII GTy'rAC
Cuts at: 0 777 1188
Size: 777 411
KasI G'GCGC_C
Cuts at: 0 784 1188
Size: 784 404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1188
Size: 479 709
MslI CAynn'nnrTG
Cuts at: 0 145 298 979 1188
Size: 145 153 681 209
Fragments arranged by size:
681 209 153 145
MspA1I CmG'CkG
Cuts at: 0 361 369 518 1188
Size: 361 8 149 670
Fragments arranged by size:
670 361 149 8
NarI GG'CG_CC
Cuts at: 0 785 1188
Size: 785 403
NcoI C'CATG_G
Cuts at: 0 974 1188
Size: 974 214
NspI r_CATG'y
Cuts at: 0 447 1188
Size: 447 741
PasI CC'CwG_GG
Cuts at: 0 180 1188
Size: 180 1008
PpuMI rG'GwC_Cy
Cuts at: 0 30 93 241 1188
Size: 30 63 148 947
Fragments arranged by size:
947 148 63 30
PstI C_TGCA'G
Cuts at: 0 227 1188
Size: 227 961
SacI G_AGCT'C
Cuts at: 0 80 1188
Size: 80 1108
SfcI C'TryA_G
Cuts at: 0 223 1188
Size: 223 965
SfoI GGC'GCC
Cuts at: 0 786 1188
Size: 786 402
SmlI C'TyrA_G
Cuts at: 0 478 526 573 961 1188
Size: 478 48 47 388 227
Fragments arranged by size:
478 388 227 48 47
SphI G_CATG'C
Cuts at: 0 447 1188
Size: 447 741
StuI AGG'CCT
Cuts at: 0 407 571 992 1188
Size: 407 164 421 196
Fragments arranged by size:
421 407 196 164
StyI C'CwwG_G
Cuts at: 0 497 974 1188
Size: 497 477 214
Fragments arranged by size:
497 477 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 863 1188
Size: 863 325
TatI w'GTAC_w
Cuts at: 0 14 1188
Size: 14 1174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 383 415 454 486 838 870 1104 1136
Size: 383 32 39 32 352 32 234 32
Cuts at: 1136 1188
Size: 52
Fragments arranged by size:
383 352 234 52 39 32 32 32
32
ZraI GAC'GTC
Cuts at: 0 311 1188
Size: 311 877
Enzymes that do cut:
AatII AccI AcuI AlfI AlwNI AvaI BanI BanII
BbeI BbsI BclI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BsrDI BssSI BstEII BstYI
Bsu36I BtgI BtgZI ClaI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
KasI MmeI MslI MspA1I NarI NcoI NspI PasI
PpuMI PstI SacI SfcI SfoI SmlI SphI StuI
StyI TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BsaAI BsgI BsiEI
BsiWI BsmI BspMI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstXI BstZ17I BtsI CspCI DraI DraIII DrdI
EagI EcoRI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI