(Circular) (Six-base) MAPSORT of: pFN21AB9853.seq Check: 3667 from: 1 to: 5860 pFN21AB9853 5860 bp With 182 enzymes: SgfI * February 27, 2009 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2791 3203 1937 Size: 854 412 4594 Fragments arranged by size: 4594 854 412 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2867 3923 5307 5575 1632 Size: 1235 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1235 1056 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 4759 5736 1203 Size: 3556 977 1327 Fragments arranged by size: 3556 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 2455 3603 5175 2455 Size: 1148 1572 3140 Fragments arranged by size: 3140 1572 1148 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 2187 2571 2668 2699 2733 2934 3016 3099 Size: 694 384 97 31 34 201 82 83 Cuts at: 3099 3169 3395 3547 5656 1493 Size: 70 226 152 2109 1697 Fragments arranged by size: 2109 1697 694 384 226 201 152 97 83 82 70 34 31 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 3185 1870 Size: 73 1242 4545 Fragments arranged by size: 4545 1242 73 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2267 3139 3181 4636 618 Size: 325 217 627 480 872 42 1455 1842 Fragments arranged by size: 1842 1455 872 627 480 325 217 42 BanII G_rGCy'C Cuts at: 1391 3179 1391 Size: 1788 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4889 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2261 2295 4051 4085 1903 Size: 34 324 34 1756 34 3678 Fragments arranged by size: 3678 1756 324 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 3921 5077 1790 Size: 2131 1156 2573 Fragments arranged by size: 2573 2131 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 3591 4549 Size: 416 53 83 399 24 609 1565 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1565 958 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2690 2723 4520 1239 Size: 1451 33 1797 2579 Fragments arranged by size: 2579 1797 1451 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 3045 5854 3045 Size: 2809 3051 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2960 3667 4049 4684 Size: 53 83 186 936 1427 707 382 635 Cuts at: 4684 275 Size: 1451 Fragments arranged by size: 1451 1427 936 707 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5860 BseYI C'CCAG_C Cuts at: 5063 5559 5063 Size: 496 5364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2505 1163 Size: 573 769 4518 Fragments arranged by size: 4518 769 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3921 4006 5077 3921 Size: 85 1071 4704 Fragments arranged by size: 4704 1071 85 BsmI GAATG_Cn' Cuts at: 2916 3371 3464 2916 Size: 455 93 5312 Fragments arranged by size: 5312 455 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2951 2951 Size: 5860 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3179 3921 4006 5077 1391 Size: 399 1389 742 85 1071 2174 Fragments arranged by size: 2174 1389 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 2839 3749 5479 2839 Size: 910 1730 3220 Fragments arranged by size: 3220 1730 910 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3215 844 Size: 2371 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstXI CCAn_nnnn'nTGG Cuts at: 2296 2296 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2027 3190 3557 3942 3959 5411 5497 5509 Size: 55 1163 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1163 385 367 346 86 55 17 12 BstZ17I GTA'TAC Cuts at: 2792 2792 Size: 5860 Bsu36I CC'TnA_GG Cuts at: 3022 3022 Size: 5860 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5308 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 3376 4170 4190 3376 Size: 794 20 5046 Fragments arranged by size: 5046 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2130 2165 529 Size: 35 1566 35 4224 Fragments arranged by size: 4224 1566 35 35 DraI TTT'AAA Cuts at: 2827 3164 2827 Size: 337 5523 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3307 4196 5825 Size: 54 1425 50 275 18 1477 889 1629 Cuts at: 5825 8 Size: 43 Fragments arranged by size: 1629 1477 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2898 3790 1353 Size: 1026 519 892 3423 Fragments arranged by size: 3423 1026 892 519 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2690 2723 2867 3923 4520 5307 5575 Size: 393 1058 33 144 1056 597 787 268 Cuts at: 5575 1239 Size: 1524 Fragments arranged by size: 1524 1058 1056 787 597 393 268 144 33 EcoRI G'AATT_C Cuts at: 2571 3016 3169 3547 2571 Size: 445 153 378 4884 Fragments arranged by size: 4884 445 378 153 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2783 2783 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 5007 1678 Size: 3329 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 4501 4533 4575 Size: 32 135 32 536 32 2361 32 42 Cuts at: 4575 4607 1373 Size: 32 2626 Fragments arranged by size: 2626 2361 536 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2176 2341 3204 3450 669 Size: 988 281 238 165 863 246 3079 Fragments arranged by size: 3079 988 863 281 246 238 165 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 2341 3450 2341 Size: 1109 4751 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 2316 3459 2316 Size: 1143 4717 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2354 4974 5158 1305 Size: 1049 2620 184 2007 Fragments arranged by size: 2620 2007 1049 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4038 Fragments arranged by size: 4038 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3818 4177 4336 518 Size: 648 2652 359 159 2042 Fragments arranged by size: 2652 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2336 2519 3276 3953 5101 5346 1606 Size: 730 183 757 677 1148 245 2120 Fragments arranged by size: 2120 1148 757 730 677 245 183 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5308 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2100 3218 4763 1132 Size: 97 871 1118 1545 2229 Fragments arranged by size: 2229 1545 1118 871 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4958 PfoI T'CCnGG_A Cuts at: 2706 2706 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PstI C_TGCA'G Cuts at: 830 2042 3212 830 Size: 1212 1170 3478 Fragments arranged by size: 3478 1212 1170 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2519 2519 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 2042 3212 2042 Size: 1170 4690 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2038 2283 2323 3028 3208 4343 5024 Size: 219 993 245 40 705 180 1135 681 Cuts at: 5024 5215 826 Size: 191 1471 Fragments arranged by size: 1471 1135 993 705 681 245 219 191 180 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 2100 3218 1132 Size: 968 1118 3774 Fragments arranged by size: 3774 1118 968 SspI AAT'ATT Cuts at: 5 52 2251 3784 5 Size: 47 2199 1533 2081 Fragments arranged by size: 2199 2081 1533 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 3299 513 Size: 552 2234 3074 Fragments arranged by size: 3074 2234 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2360 3887 3904 4057 4242 4687 325 Size: 1255 780 1527 17 153 185 445 1498 Fragments arranged by size: 1527 1498 1255 780 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2992 2997 4106 Size: 274 80 33 51 1443 1015 5 1109 Cuts at: 4106 5818 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1109 1015 274 138 80 51 33 5 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5820 5852 5820 Size: 32 5828 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 2590 3196 2590 Size: 606 5254 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI