Restriction map for
pFN21AB9848
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AgeI Alw44I AlwI
AlwNI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrDI
BsrGI Bst1107I BstAPI BstBI BstYI
BstZ17I CelII ClaI CpoI DpnII
DraI DraIII DrdI DsaI EagI
EclXI Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MamI
MboI MfeI MflI MluI MroI
MseI MunI NcoI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI Sau3AI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 801 GACGTC GACGT^C
AflIII 296 ACATGT A^C[GA][CT]GT
AhdI 675 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 112 CCTGAGG CC^T[ACGT]AGG
Aor51HI 878 AGCGCT AGC^GCT
AspEI 675 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 546 CCTAGG C^CTAGG
BbeI 1012 GGCGCC GGCGC^C
BbsI 964 GTCTTC GAAGAC(2/6)
BfaI 547 CTAG C^TAG
BlnI 546 CCTAGG C^CTAGG
BpuAI 964 GTCTTC GAAGAC(2/6)
BsaI 470 GAGACC GGTCTC(1/5)
BsgI 947 GTGCAG GTGCAG(16/14)
BspLU11I 296 ACATGT A^CATGT
BssHII 410 GCGCGC G^CGCGC
BstEII 225 GGTAACC G^GT[ACGT]ACC
BstPI 225 GGTAACC G^GT[ACGT]ACC
Bsu36I 112 CCTGAGG CC^T[ACGT]AGG
Eam1105I 675 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 865 CTCTTC CTCTTC(1/4)
Eco47III 878 AGCGCT AGC^GCT
Eco81I 112 CCTGAGG CC^T[ACGT]AGG
EcoO65I 225 GGTAACC G^GT[ACGT]ACC
KasI 1012 GGCGCC G^GCGCC
Ksp632I 865 CTCTTC CTCTTC(1/4)
MaeI 547 CTAG C^TAG
NaeI 1057 GCCGGC GCC^GGC
NarI 1012 GGCGCC GG^CGCC
NgoMIV 1057 GCCGGC G^CCGGC
SapI 864 GCTCTTC GCTCTTC(1/4)
SfaNI 540 GCATC GCATC(5/9)
SfoI 1012 GGCGCC GGC^GCC
SmlI 596 CTCAAG C^T[CT][GA]AG
SphI 504 GCATGC GCATG^C
StuI 569 AGGCCT AGG^CCT
TaqI 71 TCGA T^CGA
Tsp45I 300 GTCAC ^GT[GC]AC
TspRI 1067 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 71 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 801 GACGTC G[GA]^CG[CT]C
1012 GGCGCC
AviII 507 TGCGCA TGC^GCA
971 TGCGCA
BalI 426 TGGCCA TGG^CCA
986 TGGCCA
BsaHI 801 GACGTC G[GA]^CG[CT]C
1012 GGCGCC
BseRI 216 CTCCTC GAGGAG(10/8)
1021 GAGGAG
BsiEI 649 CGGTCG CG[GA][CT]^CG
840 CGACCG
BsmAI 470 GAGAC GTCTC(1/5)
594 GTCTC
BsrFI 925 ACCGGC [GA]^CCGG[CT]
1057 GCCGGC
BsrI 714 ACTGG ACTGG(1/-1)
860 ACTGG
BstXI 380 CCAGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
692 CCAACTGTCTGG
Cfr10I 925 ACCGGC [GA]^CCGG[CT]
1057 GCCGGC
EaeI 426 TGGCCA [CT]^GGCC[GA]
986 TGGCCA
FspI 507 TGCGCA TGC^GCA
971 TGCGCA
HgaI 630 GCGTC GACGC(5/10)
962 GCGTC
Hin1I 801 GACGTC G[GA]^CG[CT]C
1012 GGCGCC
HphI 333 TCACC GGTGA(8/7)
421 TCACC
MaeII 464 ACGT A^CGT
802 ACGT
MaeIII 226 GTAAC ^GT[ACGT]AC
300 GTCAC
MluNI 426 TGGCCA TGG^CCA
986 TGGCCA
MscI 426 TGGCCA TGG^CCA
986 TGGCCA
MslI 304 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
499 CACCCGCATG
NspI 296 ACATGT [GA]CATG^[CT]
504 GCATGC
PleI 330 GACTC GAGTC(4/5)
592 GAGTC
PpuMI 236 AGGACCT [GA]G^G[AT]CC[CT]
362 GGGACCT
PvuII 381 CAGCTG CAG^CTG
524 CAGCTG
TfiI 313 GAATC G^A[AT]TC
607 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AluI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PstI Sau96I
ScrFI SfcI SmaI StyI TseI
XmaCI XmaI