Restriction map for
pFN21AB9848
(Linear) (Six-base) MAPSORT of: KIBB9848.seq Check: 2092 from: 1 to: 1128
KIBB9848.orf
With 182 enzymes: SgfI *
March 3, 2008 11:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 830 1128
Size: 830 298
AatII G_ACGT'C
Cuts at: 0 805 1128
Size: 805 323
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 832 1128
Size: 427 405 296
Fragments arranged by size:
427 405 296
AfeI AGC'GCT
Cuts at: 0 880 1128
Size: 880 248
AflIII A'CryG_T
Cuts at: 0 296 1128
Size: 296 832
AhdI GACnn_n'nnGTC
Cuts at: 0 680 1128
Size: 680 448
ApaI G_GGCC'C
Cuts at: 0 175 271 734 1128
Size: 175 96 463 394
Fragments arranged by size:
463 394 175 96
AvaI C'yCGr_G
Cuts at: 0 264 432 774 1041 1078 1128
Size: 264 168 342 267 37 50
Fragments arranged by size:
342 267 264 168 50 37
AvrII C'CTAG_G
Cuts at: 0 546 1128
Size: 546 582
BanI G'GyrC_C
Cuts at: 0 376 654 928 1012 1128
Size: 376 278 274 84 116
Fragments arranged by size:
376 278 274 116 84
BanII G_rGCy'C
Cuts at: 0 112 175 271 723 734 1076 1128
Size: 112 63 96 452 11 342 52
Fragments arranged by size:
452 342 112 96 63 52 11
BbeI G_GCGC'C
Cuts at: 0 1016 1128
Size: 1016 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 957 1128
Size: 957 171
Bme1580I G_kGCm'C
Cuts at: 0 175 271 379 489 659 734 1128
Size: 175 96 108 110 170 75 394
Fragments arranged by size:
394 175 170 110 108 96 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 724 1128
Size: 724 404
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 1128
Size: 369 759
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 721 898 1128
Size: 167 554 177 230
Fragments arranged by size:
554 230 177 167
Bpu10I CC'TnA_GC
Cuts at: 0 566 666 1128
Size: 566 100 462
Fragments arranged by size:
566 462 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1128
Size: 581 547
BsaI GGTCTCn'nnnn_
Cuts at: 0 464 1128
Size: 464 664
BsaHI Gr'CG_yC
Cuts at: 0 802 1013 1128
Size: 802 211 115
Fragments arranged by size:
802 211 115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 1128
Size: 115 30 983
Fragments arranged by size:
983 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 207 1036 1128
Size: 207 829 92
Fragments arranged by size:
829 207 92
BseYI C'CCAG_C
Cuts at: 0 168 1030 1128
Size: 168 862 98
Fragments arranged by size:
862 168 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1128
Size: 968 160
BsiEI CG_ry'CG
Cuts at: 0 652 843 1128
Size: 652 191 285
Fragments arranged by size:
652 285 191
Bsp1286I G_dGCh'C
Cuts at: 0 112 175 271 379 489 659 723 734
Size: 112 63 96 108 110 170 64 11
Cuts at: 734 1076 1128
Size: 342 52
Fragments arranged by size:
342 170 112 110 108 96 64 63
52 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 238 830 1128
Size: 83 155 592 298
Fragments arranged by size:
592 298 155 83
BsrFI r'CCGG_y
Cuts at: 0 925 1057 1128
Size: 925 132 71
Fragments arranged by size:
925 132 71
BssHII G'CGCG_C
Cuts at: 0 410 1128
Size: 410 718
BssSI C'ACGA_G
Cuts at: 0 105 354 1006 1128
Size: 105 249 652 122
Fragments arranged by size:
652 249 122 105
BstEII G'GTnAC_C
Cuts at: 0 225 1128
Size: 225 903
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 699 1128
Size: 387 312 429
Fragments arranged by size:
429 387 312
Bsu36I CC'TnA_GG
Cuts at: 0 113 1128
Size: 113 1015
BtsI GCAGTG_nn'
Cuts at: 0 1073 1128
Size: 1073 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 463 498 1128
Size: 463 35 630
Fragments arranged by size:
630 463 35
EaeI y'GGCC_r
Cuts at: 0 426 986 1128
Size: 426 560 142
Fragments arranged by size:
560 426 142
EarI CTCTTCn'nnn_
Cuts at: 0 871 1128
Size: 871 257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 721 1128
Size: 721 407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 427 721 832 898 1128
Size: 167 260 294 111 66 230
Fragments arranged by size:
294 260 230 167 111 66
EcoO109I rG'GnC_Cy
Cuts at: 0 212 237 268 363 1128
Size: 212 25 31 95 765
Fragments arranged by size:
765 212 95 31 25
FspI TGC'GCA
Cuts at: 0 509 973 1128
Size: 509 464 155
Fragments arranged by size:
509 464 155
FspAI rTGC'GCAy
Cuts at: 0 509 1128
Size: 509 619
HaeII r_GCGC'y
Cuts at: 0 49 442 882 909 1016 1128
Size: 49 393 440 27 107 112
Fragments arranged by size:
440 393 112 107 49 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 666 698 1128
Size: 115 32 519 32 430
Fragments arranged by size:
519 430 115 32 32
KasI G'GCGC_C
Cuts at: 0 1012 1128
Size: 1012 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 447 1128
Size: 447 681
MscI TGG'CCA
Cuts at: 0 428 988 1128
Size: 428 560 140
Fragments arranged by size:
560 428 140
MslI CAynn'nnrTG
Cuts at: 0 308 503 1128
Size: 308 195 625
Fragments arranged by size:
625 308 195
MspA1I CmG'CkG
Cuts at: 0 10 138 168 383 526 836 1128
Size: 10 128 30 215 143 310 292
Fragments arranged by size:
310 292 215 143 128 30 10
NaeI GCC'GGC
Cuts at: 0 1059 1128
Size: 1059 69
NarI GG'CG_CC
Cuts at: 0 1013 1128
Size: 1013 115
NgoMIV G'CCGG_C
Cuts at: 0 1057 1128
Size: 1057 71
NspI r_CATG'y
Cuts at: 0 300 508 1128
Size: 300 208 620
Fragments arranged by size:
620 300 208
PciI A'CATG_T
Cuts at: 0 296 1128
Size: 296 832
PpuMI rG'GwC_Cy
Cuts at: 0 237 363 1128
Size: 237 126 765
Fragments arranged by size:
765 237 126
PspOMI G'GGCC_C
Cuts at: 0 171 267 730 1128
Size: 171 96 463 398
Fragments arranged by size:
463 398 171 96
PstI C_TGCA'G
Cuts at: 0 80 143 158 290 905 958 1128
Size: 80 63 15 132 615 53 170
Fragments arranged by size:
615 170 132 80 63 53 15
PvuII CAG'CTG
Cuts at: 0 383 526 1128
Size: 383 143 602
Fragments arranged by size:
602 383 143
SapI GCTCTTCn'nnn_
Cuts at: 0 871 1128
Size: 871 257
SfcI C'TryA_G
Cuts at: 0 76 139 154 286 901 954 1128
Size: 76 63 15 132 615 53 174
Fragments arranged by size:
615 174 132 76 63 53 15
SfoI GGC'GCC
Cuts at: 0 1014 1128
Size: 1014 114
SmaI CCC'GGG
Cuts at: 0 266 434 776 1080 1128
Size: 266 168 342 304 48
Fragments arranged by size:
342 304 266 168 48
SmlI C'TyrA_G
Cuts at: 0 596 1128
Size: 596 532
SphI G_CATG'C
Cuts at: 0 508 1128
Size: 508 620
StuI AGG'CCT
Cuts at: 0 571 1128
Size: 571 557
StyI C'CwwG_G
Cuts at: 0 257 546 572 1016 1128
Size: 257 289 26 444 112
Fragments arranged by size:
444 289 257 112 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 350 1128
Size: 350 778
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 1128
Size: 340 32 756
Fragments arranged by size:
756 340 32
XmaI C'CCGG_G
Cuts at: 0 264 432 774 1078 1128
Size: 264 168 342 304 50
Fragments arranged by size:
342 304 264 168 50
ZraI GAC'GTC
Cuts at: 0 803 1128
Size: 803 325
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI ApaI AvaI
AvrII BanI BanII BbeI BbsI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI Bsp1286I BspMI BsrFI BssHII BssSI BstEII
BstXI Bsu36I BtsI CspCI EaeI EarI EciI Eco57MI
EcoO109I FspI FspAI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PpuMI
PspOMI PstI PvuII SapI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TstI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI ClaI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI HincII
HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI